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公开(公告)号：US09939454B2
公开(公告)日：2018-04-10
申请号：US14937736
申请日：2015-11-10
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jeffrey Dzubay , Kyle Gee , Vladimir Martin , Aleksey Rukavishnikov , Daniel Beacham
IPC: G01N33/84 , G01N33/58 , C07K1/13 , C09B7/00 , C09B11/12 , C09B11/28 , C09B57/00 , C09B69/00 , G01N31/22 , C09B11/24 , G01N33/50 , G01N33/80 , C07D311/82 , C07D311/90 , C07D405/12 , C07D413/04 , C07D491/22 , C07D209/14 , C09K11/06 , C07F5/02
CPC classification number: G01N33/84 , C07D209/14 , C07D311/82 , C07D311/90 , C07D405/12 , C07D413/04 , C07D491/22 , C07F5/02 , C07K1/13 , C09B7/00 , C09B11/12 , C09B11/24 , C09B11/28 , C09B57/00 , C09B69/00 , C09K11/06 , G01N31/22 , G01N33/5005 , G01N33/5058 , G01N33/5091 , G01N33/582 , G01N33/80 , G01N2333/245 , Y10T436/143333
Abstract: A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I: wherein the variables are described throughout the application.
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公开(公告)号：US09476892B2
公开(公告)日：2016-10-25
申请号：US14521346
申请日：2014-10-22
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Daniel Beacham , Kyle Gee
CPC classification number: G01N33/84 , C12Q1/02 , G01N33/6872 , G01N2333/705
Abstract: The present invention relates to methods for detecting the activity of an ion channel in a cell. The methods comprise providing a loading buffer solution to a cell that has an ion channel. The loading buffer comprises at least one thallium indicator (e.g., an environmentally sensitive, luminescent dye) and a physiological concentration of chloride ions. The methods further comprise providing a stimulus buffer to the cell, wherein the stimulus buffer comprises thallium (e.g., thallium ions). Providing the stimulus buffer causes thallium influx into the cell through the ion channel. After providing the stimulus buffer, the luminescence (e.g., fluorescence) of the dye in the cell is detected. The luminescence of the dye can change in the presence or absence of thallium. The methods may be used to measure influx or efflux of thallium through an ion channel.
Abstract translation: 本发明涉及用于检测细胞中离子通道的活性的方法。 所述方法包括向具有离子通道的电池提供加载缓冲溶液。 加载缓冲液包含至少一种铊指示剂（例如环境敏感的发光染料）和氯离子的生理浓度。 所述方法还包括向细胞提供刺激缓冲液，其中刺激缓冲液包含铊（例如铊离子）。 提供刺激缓冲液使铊通过离子通道流入细胞。 在提供刺激缓冲液之后，检测细胞中染料的发光（例如荧光）。 在存在或不存在铊的情况下，染料的发光可以改变。 该方法可用于测量铊通过离子通道的流入或流出。
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Patent Agency Ranking
公开(公告)号：US12140559B2
公开(公告)日：2024-11-12
申请号：US18145688
申请日：2022-12-22
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Wolfgang Hinz , John Donohue , Daniel Beacham
IPC: G01N27/414 , C09D189/00 , G01N27/28 , G01N27/30 , G01N27/407 , G01N33/483
Abstract: Various cell analysis systems of the present teachings can measure the electrical and metabolic activity of single, living cells with subcellular addressability and simultaneous data acquisition for between about 10 cells to about 500,000 cells in a single analysis. Various sensor array devices of the present teachings can have sensor arrays with between 20 million to 660 million ChemFET sensors built into a massively paralleled array and can provide for simultaneous measurement of cells with data acquisition rates in the kilohertz (kHz) range. As various ChemFET sensor arrays of the present teachings can detect chemical analytes as well detect changes in cell membrane potential, various cell analysis systems of the present teachings also provide for the controlled chemical and electrical interrogation of cells.
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公开(公告)号：US11567036B2
公开(公告)日：2023-01-31
申请号：US16568133
申请日：2019-09-11
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Wolfgang Hinz , John Donohue , Daniel Beacham
IPC: G01N27/414 , C09D189/00 , G01N27/28 , G01N27/30 , G01N27/407 , G01N33/483
Abstract: Various cell analysis systems of the present teachings can measure the electrical and metabolic activity of single, living cells with subcellular addressability and simultaneous data acquisition for between about 10 cells to about 500,000 cells in a single analysis. Various sensor array devices of the present teachings can have sensor arrays with between 20 million to 660 million ChemFET sensors built into a massively paralleled array and can provide for simultaneous measurement of cells with data acquisition rates in the kilohertz (kHz) range. As various ChemFET sensor arrays of the present teachings can detect chemical analytes as well detect changes in cell membrane potential, various cell analysis systems of the present teachings also provide for the controlled chemical and electrical interrogation of cells.
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公开(公告)号：US20210041465A1
公开(公告)日：2021-02-11
申请号：US17027391
申请日：2020-09-21
Applicant: Life Technologies Corporation
Inventor： Jeffrey DZUBAY , Kyle Gee , Vladimir Martin , Aleksey Rukavishnikov , Daniel Beacham
IPC: G01N33/84 , C07D311/82 , C07D311/90 , C07D405/12 , C07D413/04 , C07D491/22 , C07D209/14 , C09K11/06 , C07F5/02 , C07K1/13 , C09B7/00 , C09B11/12 , C09B11/28 , C09B57/00 , C09B69/00 , G01N31/22 , G01N33/50 , C09B11/24 , G01N33/58 , G01N33/80
Abstract: A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I: wherein the variables are described throughout the application.
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公开(公告)号：US20180362766A1
公开(公告)日：2018-12-20
申请号：US15955332
申请日：2018-04-17
Applicant: Life Technologies Corporation
Inventor： Kyle Gee , Upinder Singh , Aleksey Rukavishnikov , Daniel Beacham , Shih-Jung Huang , Michael Janes , Wenjun Zhou , Judith Berlier
Abstract: Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.
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公开(公告)号：US20180238912A1
公开(公告)日：2018-08-23
申请号：US15899021
申请日：2018-02-19
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jeffrey DZUBAY , Kyle Gee , Vladimir Martin , Aleksey Rukavishnikov , Daniel Beacham
IPC: G01N33/84 , C09B11/24 , C07K1/13 , C09B7/00 , C09B11/12 , C09B11/28 , C09B57/00 , G01N31/22 , G01N33/50 , G01N33/58 , C09K11/06 , C07D311/82 , C07D311/90 , C07D405/12 , C07D413/04 , C07D491/22 , C07D209/14 , C07F5/02 , C09B69/00 , G01N33/80
CPC classification number: G01N33/84 , C07D209/14 , C07D311/82 , C07D311/90 , C07D405/12 , C07D413/04 , C07D491/22 , C07F5/02 , C07K1/13 , C09B7/00 , C09B11/12 , C09B11/24 , C09B11/28 , C09B57/00 , C09B69/00 , C09K11/06 , G01N31/22 , G01N33/5005 , G01N33/5058 , G01N33/5091 , G01N33/582 , G01N33/80 , G01N2333/245 , Y10T436/143333
Abstract: A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I: wherein the variables are described throughout the application.
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