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    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME
    12.
    发明申请
    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME 审中-公开

    公开(公告)号：US20180305673A1

    公开(公告)日：2018-10-25

    申请号：US15959839

    申请日：2018-04-23

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Peter Vander Horn ,  Theo Nikiforov ,  Guobin Luo ,  Mindy Landes ,  Daniel Mazur ,  Eileen Tozer ,  Tommie Lloyd Lincecum

    IPC: C12N9/12 ,  C12Q1/686 ,  C12Q1/6844

    CPC classification number: C12N9/1252 ,  C12Q1/6846 ,  C12Q1/686 ,  C12Y207/07007

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.

    Polymerase Compositions, Methods of Making and Using Same
    14.
    发明申请
    Polymerase Compositions, Methods of Making and Using Same 有权
    聚合酶组合物，制备和使用方法

    公开(公告)号：US20150368626A1

    公开(公告)日：2015-12-24

    申请号：US14754203

    申请日：2015-06-29

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Peter Vander Horn ,  Theo Nikiforov ,  Guobin Luo ,  Mindy Landes ,  Daniel Mazur ,  Eileen Tozer ,  Tommie Lincecum

    IPC: C12N9/12 ,  C12Q1/68

    CPC classification number: C12N9/1252 ,  C12Q1/6846 ,  C12Q1/686 ,  C12Y207/07007

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.

    Abstract translation: 本公开提供了可用于核酸聚合的组合物，方法，试剂盒，系统和装置。 特别地，提供了允许核酸扩增的修饰的聚合酶及其生物活性片段。 一方面，本公开涉及可用于核酸测序，基因分型，拷贝数变异分析，配对末端测序和其他形式遗传分析的修饰聚合酶。 在一些方面，本公开涉及可用于产生用于各种下游过程的核酸文库或核酸模板的修饰聚合酶。 在一些方面，本公开涉及可以跨聚合酶类或家族转移的同源氨基酸突变的鉴定，以提供具有改变的催化性质的新型聚合酶。 在一些方面，本公开提供与参考聚合酶相比具有增强的催化性质的修饰聚合酶。

    Polymerase compositions, methods of making and using same
    15.
    发明授权
    Polymerase compositions, methods of making and using same 有权

    公开(公告)号：US11821011B2

    公开(公告)日：2023-11-21

    申请号：US17445047

    申请日：2021-08-13

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Peter Vander Horn ,  Daniel Mazur ,  Theo Nikiforov ,  Mindy Landes ,  Eileen Tozer

    IPC: C12N9/12 ,  C12Q1/68 ,  C12Q1/686 ,  C12Q1/6869

    CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Q1/6869

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.

    POLYMERASE COMPOSITIONS AND METHODS OF MAKING AND USING SAME
    17.
    发明申请
    POLYMERASE COMPOSITIONS AND METHODS OF MAKING AND USING SAME 有权

    公开(公告)号：US20220307071A1

    公开(公告)日：2022-09-29

    申请号：US17725369

    申请日：2022-04-20

    Applicant: Life Technologies Corporation

    Inventor： Daniel Mazur ,  Eileen Tozer ,  Sihong Chen ,  Peter Vander Horn ,  Tommie Lincecum

    IPC: C12Q1/686 ,  C12Q1/6869 ,  C12N9/12

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof, such as modified Taq polymerases, are provided that allow for improved nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having improved thermostability, accuracy, processivity and/or read length as compared to a referenceTaq polymerase. In some aspects, the disclosure relates to modified polymerases or biologically active fragments thereof, useful for amplification methods, and in practically illustrative embodiments, emulsion PCR.

    Polymerase compositions and methods of making and using same
    18.
    发明授权
    Polymerase compositions and methods of making and using same 有权

    公开(公告)号：US11319584B2

    公开(公告)日：2022-05-03

    申请号：US16947742

    申请日：2020-08-14

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Daniel Mazur ,  Eileen Tozer ,  Sihong Chen ,  Peter Vander Horn ,  Tommie Lincecum

    IPC: C12N9/12 ,  C12Q1/686 ,  C12Q1/6869

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof, such as modified Taq polymerases, are provided that allow for improved nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having improved thermostability, accuracy, processivity and/or read length as compared to a referenceTaq polymerase. In some aspects, the disclosure relates to modified polymerases or biologically active fragments thereof, useful for amplification methods, and in practically illustrative embodiments, emulsion PCR.

    Library preparation methods and compositions and uses therefor
    20.
    发明授权
    Library preparation methods and compositions and uses therefor 有权

    公开(公告)号：US11001836B2

    公开(公告)日：2021-05-11

    申请号：US16024175

    申请日：2018-06-29

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Mark Andersen ,  Daniel Mazur ,  Sihong Chen ,  Guobin Luo ,  Xinzhan Peng

    IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/686 ,  C40B20/04 ,  C40B50/06 ,  C40B40/06

    Abstract: Provided are methods for preparing a library of target nucleic acid sequences, as well as compositions and uses therefor. Methods comprise contacting a nucleic acid sample with a plurality of adaptors capable of amplification of one or more target nucleic acid sequences under conditions wherein the target nucleic acid(s) undergo a first amplification; digesting the resulting first amplification products; repairing the digested target amplicons; and amplifying the repaired products in a second amplification, thereby producing a library of target nucleic acid sequence. Each of the plurality of adaptor compositions comprise a handle and a targeted nucleic acid sequence and optionally one or more tag sequences. Provided methods may be carried out in a single, addition only workflow reaction, allowing for rapid production of highly multiplexed targeted libraries, optionally including unique tag sequences. Resulting library compositions are useful for a variety of applications, including sequencing applications.

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