公开(公告)号：US09914964B2

公开(公告)日：2018-03-13

申请号：US14523410

申请日：2014-10-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Parul Angrish ,  Zhiwei Yang

IPC: C12Q1/68 ,  C07D207/16 ,  C07C41/22 ,  C07C211/63 ,  C07C303/28 ,  C07D211/60 ,  C11D1/88 ,  C12N9/12 ,  C12N9/96

CPC classification number: C12Q1/686 ,  C07C41/22 ,  C07C211/63 ,  C07C303/28 ,  C07D207/16 ,  C07D211/60 ,  C11D1/88 ,  C12N9/1252 ,  C12N9/96

Abstract: This disclosure relates to novel compounds for use in various compositions, kits and methods, including, for example, use in polymerase storage buffers and in nucleic acid synthesis or amplification reactions such as a polymerase chain reaction (PCR). Methods for preparing the novel compounds are also described.

公开(公告)号：US09901642B2

公开(公告)日：2018-02-27

申请号：US14340395

申请日：2014-07-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Zhiwei Yang ,  Parul Angrish ,  Xavier de Mollerat du Jeu ,  Kristin Wiederholt ,  Tao Wu

IPC: C07C227/06 ,  C07D211/28 ,  C07D211/58 ,  C12N15/88 ,  A61K48/00 ,  C07C229/08 ,  C12N15/113 ,  A61K47/54 ,  A61K47/48

CPC classification number: A61K47/54 ,  A61K47/543 ,  A61K48/0008 ,  C07C227/06 ,  C07C229/08 ,  C07D211/28 ,  C07D211/58 ,  C12N15/1137 ,  C12N15/88 ,  C12N2310/11 ,  C12N2310/141 ,  C07C229/26

Abstract: There are provided for herein novel amine-containing transfection compounds and methods for making and using same. The compounds are generally obtained by reacting a primary amine with an unsaturated compound. Transfection complexes made using the amine-containing transfection compounds in combination with additional compounds to encapsulate biologically active agents such as nucleic acids are also provided for herein. Methods of using the transfection complexes for the in vivo or in vitro delivery of biologically active agents are also described. The transfection complexes of the present invention are highly potent, thereby allowing effective modulation of a biological activity at relatively low doses compared to analogous transfection compounds known in the art.

公开(公告)号：US09493414B2

公开(公告)日：2016-11-15

申请号：US14250154

申请日：2014-04-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Parul Angrish ,  Zhiwei Yang ,  Jonathan Wang

IPC: C12Q1/68 ,  C07D211/60 ,  C07C229/12 ,  C07C229/24 ,  C07D207/16

CPC classification number: C12Q1/6848 ,  C07C229/12 ,  C07C229/24 ,  C07D207/16 ,  C07D211/60 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6876 ,  C12Q2600/158 ,  C12Q2527/125

Abstract: This disclosure relates to novel detergents for use in various procedures including, for example, nucleic acid amplification reactions such as polymerase chain reaction (PCR). Methods for preparing the modified detergents are also described.