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    Methods and Systems for a Digital PCR Experiment Designer
    11.
    发明申请
    Methods and Systems for a Digital PCR Experiment Designer 审中-公开
    数字PCR实验设计器的方法和系统

    公开(公告)号：US20150278437A1

    公开(公告)日：2015-10-01

    申请号：US14428312

    申请日：2013-09-13

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Nivedita Sumi Majumdar ,  Thomas Wessel ,  David C. Woo ,  Marcin Sikora ,  Gordon A. Janaway ,  Shweta Raizada ,  Joanna Lankester ,  Jeffrey A. Marks ,  Daniel Wessel

    IPC: G06F19/20 ,  C12Q1/68

    CPC classification number: G16B25/00 ,  C12Q1/686 ,  G16B40/00

    Abstract: A computer-implemented method for designing a digital PCR (dPCR) experiment is provided. The method includes receiving, from a user, a selection of optimization type. The optimization type may be maximizing the dynamic range, minimizing the number of substrates including reaction sites needed for the experiment, determining a dilution factor, or determining the lower limit of detection, for example. The method further includes receiving, from the user, a precision measure for an experiment, and a minimum concentration of a target in a reaction site for the experiment. The method also includes determining a set of dPCR experiment design factors for the experiment based on the optimization type. The set of dPCR experiment design factors is then displayed to the user.

    Abstract translation: 提供了一种用于设计数字PCR（dPCR）实验的计算机实现方法。 该方法包括从用户接收优化类型的选择。 优化类型可以使动态范围最大化，例如最小化包括实验所需的反应位点，确定稀释因子或确定检测下限的底物数量。 该方法还包括从用户接收实验的精确度量，以及用于实验的反应部位中靶的最小浓度。 该方法还包括基于优化类型确定实验的一组dPCR实验设计因子。 然后将该组dPCR实验设计因子显示给用户。

    METHODS AND SYSTEMS FOR BACKGROUND SUBTRACTION IN AN IMAGE
    12.
    发明申请
    METHODS AND SYSTEMS FOR BACKGROUND SUBTRACTION IN AN IMAGE 有权
    背景技术在图像中的方法和系统

    公开(公告)号：US20140233816A1

    公开(公告)日：2014-08-21

    申请号：US14347960

    申请日：2012-09-28

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Francis T. Cheng ,  Thomas Wessel

    IPC: G06T7/00

    CPC classification number: G06T7/0012 ,  G06T5/50 ,  G06T7/70 ,  G06T2207/20068 ,  G06T2207/30024 ,  G06T2207/30072

    Abstract: A method for improving image quality is provided. The method includes receiving image data of a substrate, wherein the image data is generated by imaging the substrate, and an image is generated from the image data. The method further includes generating a background representation from a background noise portion of the image, wherein the background portion includes signal information undesired for further processing and generating a background subtracted image by subtracting the background rep resentation from the image. In this way, a separate background image is not needed to subtract the background from the image including the regions-of-interest to improve image quality.

    Abstract translation: 提供了一种提高图像质量的方法。 该方法包括接收基板的图像数据，其中通过对基板进行成像而生成图像数据，并且从图像数据生成图像。 所述方法还包括从所述图像的背景噪声部分生成背景表示，其中所述背景部分包括不期望用于进一步处理的信号信息，并且通过从所述图像中减去所述背景重现来生成背景减去图像。 以这种方式，不需要单独的背景图像从包括感兴趣区域的图像中减去背景以提高图像质量。

    Methods and systems for instrument validation
    15.
    发明授权
    Methods and systems for instrument validation 有权

    公开(公告)号：US10648912B2

    公开(公告)日：2020-05-12

    申请号：US15016485

    申请日：2016-02-05

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Thomas Wessel ,  Yong Chu ,  Jacob Freudenthal ,  David Woo

    IPC: G01N21/64 ,  G01N21/27 ,  G16B40/00

    Abstract: In one exemplary embodiment, a method for validating an instrument is provided. The method includes receiving amplification data from a validation plate to generate a plurality of amplification curves. The validation plate includes a sample of a first quantity and a second quantity, and each amplification curve includes an exponential region. The method further includes determining a set of fluorescence thresholds based on the exponential regions of the plurality of amplification curves and determining, for each fluorescence threshold of the set, a first set of cycle threshold (Ct) values of amplification curves generated from the samples of the first quantity and a second set of Ct values of amplification curves generated from the samples of the second quantity. The method includes calculating if the first and second quantities are sufficiently distinguishable based on Ct values at each of the plurality of fluorescence thresholds.

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