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11.发明授权公开(公告)号：US06818760B1
公开(公告)日：2004-11-16
申请号：US09680889
申请日：2000-10-06
申请人： Douglas A. Spicer , Karin A. Hughes , Robert J. Kaiser , James E. Mahoney , Amy L. Springer , Mark L. Stolowitz , Carl H. D. Weissman
发明人： Douglas A. Spicer , Karin A. Hughes , Robert J. Kaiser , James E. Mahoney , Amy L. Springer , Mark L. Stolowitz , Carl H. D. Weissman
IPC分类号： C07H2102
CPC分类号： C12Q1/6869 , C12Q1/6844 , C12Q2563/107 , C12Q2563/149 , C12Q2565/137
摘要： This invention provides methods for removing unincorporated dye-labeled molecules from a mixture that includes dye-labeled polynucleotides or other polymers and the unincorporated dye-labeled molecules. The methods involve adsorbing the unincorporated dye-labeled molecules into a plurality of particles that are made up of one or more porous hydrophobic materials that are encapsulated in a hydrophilic matrix.
摘要翻译： 本发明提供了从包含染料标记的多核苷酸或其它聚合物的混合物和未掺入的染料标记分子的混合物中除去未结合的染料标记的分子的方法。 所述方法包括将未结合的染料标记的分子吸附到多个由包封在亲水基质中的一种或多种多孔疏水性材料构成的颗粒中。
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公开(公告)号：US6031117A
公开(公告)日：2000-02-29
申请号：US272978
申请日：1999-03-19
IPC分类号： C07F9/6596 , C07H21/00 , C07F5/04
CPC分类号： C07H21/00 , C07F9/6596
摘要： The present invention provides arylboronic acid reagents useful for incorporation into modified oligonucleotides and polynucleotides. The modified oligonucleotides and polynucleotides thus produced are useful in bioconjugation reactions for the immobilization and purification of macromolecules.
摘要翻译： 本发明提供可用于掺入修饰的寡核苷酸和多核苷酸的芳基硼酸试剂。 由此产生的修饰的寡核苷酸和多核苷酸可用于大分子的固定和纯化的生物缀合反应。
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公开(公告)号：US5876938A
公开(公告)日：1999-03-02
申请号：US837340
申请日：1997-04-11
IPC分类号： C12N15/09 , C07H19/06 , C07H19/10 , C07H19/14 , C07H19/16 , C07H19/20 , C07H21/00 , C07H23/00 , C12P19/34 , C12Q1/68 , C07H19/00 , C07H21/02
摘要： Modified nucleotides and polynucleotides which are useful in hybridization assays for the detection of target genes are provided. The modified polynucleotides contain at least one boronic acid moiety which is attached to a nucleotide base in a position which does not interfere with the hydrogen bonding capabilities of that base during duplex formation. The modified polynucleotides are typically formed from naturally occurring nucleotides and one or more modified nucleotides.
摘要翻译： 提供了可用于检测靶基因的杂交测定中的修饰的核苷酸和多核苷酸。 修饰的多核苷酸含有至少一个硼酸部分，其在不影响双链体形成期间该碱基的氢键能力的位置处连接到核苷酸碱基。 修饰的多核苷酸通常由天然存在的核苷酸和一个或多个修饰的核苷酸形成。
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公开(公告)号：US5831046A
公开(公告)日：1998-11-03
申请号：US692429
申请日：1996-08-05
IPC分类号： C12N15/09 , C07H19/06 , C07H19/10 , C07H19/14 , C07H19/16 , C07H19/20 , C07H21/00 , C07H23/00 , C12P19/34 , C12Q1/68 , C07C51/10 , C07D213/00 , C07H19/00
摘要： Modified nucleotides and polynucleotides which are useful in hybridization assays for the detection of target genes are provided. The modified polynucleotides contain at least one boronic acid moiety which is attached to a nucleotide base in a position which does not interfere with the hydrogen bonding capabilities of that base during duplex formation. The modified polynucleotides are typically formed from naturally occurring nucleotides and one or more modified nucleotides.
摘要翻译： 提供了可用于检测靶基因的杂交测定中的修饰的核苷酸和多核苷酸。 修饰的多核苷酸含有至少一个硼酸部分，其在不影响双链体形成期间该碱基的氢键能力的位置处连接到核苷酸碱基。 修饰的多核苷酸通常由天然存在的核苷酸和一个或多个修饰的核苷酸形成。
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15.发明授权公开(公告)号：US06414136B1
公开(公告)日：2002-07-02
申请号：US09564117
申请日：2000-05-03
申请人： Douglas A. Spicer , Karin A. Hughes , Robert J. Kaiser , James E. Mahoney , Amy L. Springer , Mark L. Stolowitz , Carl H. D. Weissman
发明人： Douglas A. Spicer , Karin A. Hughes , Robert J. Kaiser , James E. Mahoney , Amy L. Springer , Mark L. Stolowitz , Carl H. D. Weissman
IPC分类号： C07H2102
CPC分类号： C12Q1/6869 , C12Q1/6844 , C12Q2563/107 , C12Q2563/149 , C12Q2565/137
摘要： This invention provides methods for removing unincorporated fluorescent dye-labeled molecules from a mixture that includes fluorescently-labeled polynucleotides and the unincorporated fluorescent dye-labeled molecules. The methods involve adsorbing the unincorporated fluorescent dye-labeled molecules into a plurality of particles that are made up of one or more porous hydrophobic materials that are encapsulated in a hydrophilic matrix.
摘要翻译： 本发明提供从包含荧光标记的多核苷酸和未掺入的荧光染料标记的分子的混合物中除去未结合的荧光染料标记的分子的方法。 所述方法包括将未掺入的荧光染料标记的分子吸附到由封装在亲水基质中的一种或多种多孔疏水性材料构成的多个颗粒中。
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公开(公告)号：US6013783A
公开(公告)日：2000-01-11
申请号：US272834
申请日：1999-03-19
IPC分类号： C07F9/6596 , C07H21/00 , C12N15/113 , C07H21/04 , C12P19/34
CPC分类号： C12N15/113 , C07F9/6596 , C07H21/00 , C12N2310/318 , C12N2310/33
摘要： The present invention relates to the field of nucleic acid immobilization, purification and detection and, more particularly, to boronic acid modified oligonucleotides and polynucleotides useful in bioconjugation reactions. The modified oligonucleotides and polynucleotides are useful in reactions for the immobilization and purification of macromolecules.
摘要翻译： 本发明涉及核酸固定，纯化和检测领域，更具体地涉及可用于生物缀合反应的硼酸修饰的寡核苷酸和多核苷酸。 修饰的寡核苷酸和多核苷酸可用于大分子固定和纯化的反应。
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公开(公告)号：US5877297A
公开(公告)日：1999-03-02
申请号：US956196
申请日：1997-10-22
IPC分类号： C07C229/38 , C07C233/49 , C07C233/51 , C07C237/12 , C07C237/22 , C07C255/13 , C07C259/10 , C07C271/22 , C07C281/02 , C07D207/44 , C07D207/452 , C07D207/46 , C07D213/71 , C07F5/02 , C07F5/04 , C07K5/02 , G01N33/543
CPC分类号： C07C233/51 , C07C229/38 , C07C233/49 , C07C237/12 , C07C237/22 , C07C255/13 , C07C259/10 , C07C271/22 , C07C281/02 , C07D207/452 , C07D207/46 , C07D213/71 , C07F5/025 , C07K5/0207 , G01N33/54353
摘要： Boron compound completing reagents, intermediate reagents of those reagents and methods of synthesizing these reagents. These reagents, including those shown as General Formula CII may be used, after further reactions described herein, to complex with boronic compounds, such as phenylboronic acid or derivatives thereof. ##STR1## In one embodiment, group Z comprises a spacer selected from a saturated or unsaturated chain up to about 0 to 6 carbon equivalents in length, an unbranched saturated or unsaturated chain of from about 6 to 18 carbon equivalents in length with at least one of intermediate amide and disulfide moieties, and a polyethylene glycol chain of from 3 to 12 carbon equivalents in length; group R.sub.2 is one of an alkyl and a methylene with an electronegative moiety; and BAS is a biologically active species.
摘要翻译： 硼化合物完成试剂，那些试剂的中间试剂和合成这些试剂的方法。 在本文所述的进一步反应后，可以使用这些试剂，包括通式CII所示的那些试剂与硼酸化合物如苯基硼酸或其衍生物复合。 通式CII在一个实施方案中，基团Z包括选自饱和或不饱和链长度至多约0至6个碳当量长度的间隔基，长度为约6至18个碳当量长度的无支链饱和或不饱和链， 中间体酰胺和二硫化物部分中的至少一个和长度为3至12个碳当量的聚乙二醇链; 基团R2是具有电负性部分的烷基和亚甲基之一; 而BAS是一种生物活性物种。
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公开(公告)号：US5777148A
公开(公告)日：1998-07-07
申请号：US691930
申请日：1996-08-05
IPC分类号： C07C229/38 , C07C233/49 , C07C233/51 , C07C237/12 , C07C237/22 , C07C255/13 , C07C259/10 , C07C271/22 , C07C281/02 , C07D207/44 , C07D207/452 , C07D207/46 , C07D213/71 , C07F5/02 , C07F5/04 , C07K5/02 , G01N33/543 , C07C233/31
CPC分类号： C07C233/51 , C07C229/38 , C07C233/49 , C07C237/12 , C07C237/22 , C07C255/13 , C07C259/10 , C07C271/22 , C07C281/02 , C07D207/452 , C07D207/46 , C07D213/71 , C07F5/025 , C07K5/0207 , G01N33/54353
摘要： Boron compound complexing reagents, intermediate reagents of those reagents and methods of synthesizing these reagents are disclosed. These reagents, including those shown as General Formula I and General Formula II may be used, after further reactions described herein, to complex with boronic compounds, such as phenylboronic acid or derivatives thereof. ##STR1##
摘要翻译： 公开硼化合物络合剂，这些试剂的中间试剂和合成这些试剂的方法。 在本文所述的进一步反应后，可以使用这些试剂，包括通式I和通式II所示的试剂，与硼酸化合物如苯基硼酸或其衍生物复合。 通用公式CI
通用CII -
19.发明申请Duplex Oligonucleotide Complexes and Methods for Gene Silencing by RNA Interference 有权双链寡核苷酸复合物和RNA干扰基因沉默的方法公开(公告)号：US20120322855A1
公开(公告)日：2012-12-20
申请号：US13552709
申请日：2012-07-19
IPC分类号： A61K31/713 , C12N5/07
CPC分类号： C12N15/111 , C07H19/00 , C12N15/11 , C12N15/113 , C12N2310/14 , C12N2310/315 , C12N2310/321 , C12N2310/322 , C12N2310/346 , C12N2310/3515 , C12N2320/32 , C12N2330/30 , C12N2310/3521
摘要： Provided herein are duplex oligonucleotide complexes which can be administered to a cell, tissue or organism to silence a target gene without the aid of a transfection reagent(s). The duplex oligonucleotide complexes of the disclosure include a conjugate moiety that facilitates delivery to a cell, tissue or organism.
摘要翻译： 本文提供了可以在不借助转染试剂的情况下向细胞，组织或生物体施用以沉默靶基因的双链寡核苷酸复合物。 本公开的双链寡核苷酸复合物包括有助于递送至细胞，组织或生物体的缀合物部分。
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公开(公告)号：US08026349B2
公开(公告)日：2011-09-27
申请号：US11660589
申请日：2005-08-18
CPC分类号： C07H21/04
摘要： Methods and compositions for making nucleoside phosphoramidites and nucleic acids, including mono-, di-, and polynucleotides, comprising a linker covalently attached to a levulinyl moiety are provided. A levulinyl-protected linking moiety affords an orthogonal approach to modifying a polynucleotide during or after solid phase synthesis with a molecule of interest, for example, a conjugate or a dye.
摘要翻译： 提供了制备核苷亚磷酰胺和核酸（包括单 - ，二 - 和多核苷酸）的方法和组合物，其包含共价连接到乙酰乙酰部分的连接体。 乙酰乙酰基受保护的连接部分提供了在与感兴趣的分子（例如缀合物或染料）固相合成期间或之后修饰多核苷酸的正交方法。
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