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23 条记录 (耗时 0.026 秒)

    METHODS FOR FRACTIONATING AND PROCESSING MICROPARTICLES FROM BIOLOGICAL SAMPLES AND USING THEM FOR BIOMARKER DISCOVERY
    11.
    发明申请
    METHODS FOR FRACTIONATING AND PROCESSING MICROPARTICLES FROM BIOLOGICAL SAMPLES AND USING THEM FOR BIOMARKER DISCOVERY 审中-公开
    从生物样品中分离和处理微生物并将其用于生物识别发现的方法

    公开(公告)号：US20130052647A1

    公开(公告)日：2013-02-28

    申请号：US13578464

    申请日：2011-02-10

    申请人： Marianna Goldrick ,  Lance Ford

    发明人： Marianna Goldrick ,  Lance Ford

    IPC分类号： C12Q1/68 ,  C07K1/14 ,  C12S3/18 ,  C07H1/08 ,  C12Q1/02 ,  C12N5/00

    CPC分类号： C07K1/34 ,  C12Q1/6806 ,  C12Q2563/161

    摘要： Described herein are methods, devices, and compositions for fractionation and processing of microparticles from biological samples, and to methods for obtaining and using the microparticles for biomarker discovery. Biological samples include cell-free fluids, for example blood plasma, blood serum, cerebrospinal fluid, urine, and saliva, as well as conditioned media. Conditioned media is the liquid growth media used to propagate cells in vitro. Purification of microparticles from cell-free fluids is challenging, typically accomplished by prolonged ultracentrifugation. Described herein is an alternative method for efficiently harvesting and processing microparticles from cell-free fluids and from conditioned media. Embodiments described herein relate to use of the microparticles and their contents recovered from conditioned media derived from propagation of human and animal cells, as a source of biomarkers for diagnosis and prognosis of diseases and pathological conditions.

    摘要翻译： 本文描述了用于从生物样品中分离和加工微粒的方法，装置和组合物，以及获得和使用微粒用于生物标志物发现的方法。 生物样品包括无细胞流体，例如血浆，血清，脑脊髓液，尿液和唾液以及条件培养基。 条件培养基是用于在体外繁殖细胞的液体生长培养基。 从无细胞流体中纯化微粒是有挑战性的，通常通过长时间超速离心来实现。 本文描述的是用于从无细胞流体和条件培养基有效收获和加工微粒的替代方法。 本文所述的实施方案涉及从源自人和动物细胞繁殖的条件培养基中回收的微粒及其内容物作为疾病和病理状态的诊断和预后的生物标志物的来源。

    Methods and compositions for preparing tissue samples for RNA extraction
    15.
    发明授权
    Methods and compositions for preparing tissue samples for RNA extraction 有权
    用于制备用于RNA提取的组织样品的方法和组合物

    公开(公告)号：US07250270B2

    公开(公告)日：2007-07-31

    申请号：US10462091

    申请日：2003-06-13

    申请人： Marianna Goldrick ,  Juanita C. Gonzales

    发明人： Marianna Goldrick ,  Juanita C. Gonzales

    IPC分类号： G01N1/30 ,  G01N33/48

    CPC分类号： G01N1/30

    摘要： The present invention concerns the methods and compositions for preparing a tissue section or biological sample, particularly to preserve RNA in the section or sample, by not exposing or contacting the sample or section to a solution that is composed of mostly water. Tissue sections can be fixed, stained, and dehydrated for subsequent manipulation, including laser capture microdissection (LCM) for further analysis using methods and/or compositions of the invention.

    摘要翻译： 本发明涉及用于制备组织切片或生物样品的方法和组合物，特别是通过不将样品或部分暴露或接触到由大多数水组成的溶液而将部分或样品中的RNA保留。 组织切片可以固定，染色和脱水以用于随后的操作，包括使用本发明的方法和/或组合物进一步分析的激光捕获显微切割（LCM）。

    Process and reagents for extraction of RNA from fractionated blood leukocytes
    16.
    发明申请
    Process and reagents for extraction of RNA from fractionated blood leukocytes 审中-公开
    从分离的血液白细胞中提取RNA的方法和试剂

    公开(公告)号：US20050208501A1

    公开(公告)日：2005-09-22

    申请号：US10801982

    申请日：2004-03-16

    申请人： Marianna Goldrick

    发明人： Marianna Goldrick

    IPC分类号： C12Q1/68 ,  G01N33/543

    CPC分类号： C12Q1/6806 ,  C12Q2531/113 ,  C12Q2527/137

    摘要： The current invention relates generally to methods for rapid fractionation of WBCs from whole blood using a proprietary leukocyte depletion filter, which concentrates and partially purifies the WBCs, and for stabilizing the RNA patterns in the fractionated cells. The invention further relates to methods of extracting the RNA from the fractionated cells and using such nucleic acids in any of a variety of molecular biology procedures including, for example, expression profiling.

    摘要翻译： 本发明一般涉及使用专有白细胞消耗过滤器从全血中快速分级WBC的方法，所述专利白细胞消耗过滤器浓缩并部分纯化WBC，并稳定分级细胞中的RNA图案。 本发明还涉及从分级细胞中提取RNA并使用这些核酸在多种分子生物学方法中包括例如表达谱的方法。

    LATERAL FLOW DETECTION OF TARGET SEQUENCES
    18.
    发明申请
    LATERAL FLOW DETECTION OF TARGET SEQUENCES 审中-公开
    目标序列的侧流检测

    公开(公告)号：US20140170649A1

    公开(公告)日：2014-06-19

    申请号：US14039908

    申请日：2013-09-27

    申请人： Joseph F. Krebs ,  Paul Morrison ,  Marianna Goldrick

    发明人： Joseph F. Krebs ,  Paul Morrison ,  Marianna Goldrick

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/686 ,  C12Q2561/101 ,  C12Q2563/131 ,  C12Q2565/625

    摘要： Provided is a sensitive method to specifically detect nucleic acid sequences using a combination of DNA replication based signal amplification (e.g., PCR) and lateral flow analyte detection. The disclosed method uses a dual-labeled DNA probe complementary to a region of the target DNA sequence. Cleavage of the probe, caused by the presence of the amplified DNA target, is detected using lateral flow methods.

    摘要翻译： 提供了使用基于DNA复制的信号扩增（例如PCR）和侧流分析物检测的组合来特异性检测核酸序列的敏感方法。 所公开的方法使用与靶DNA序列的区域互补的双标记DNA探针。 使用横向流动方法检测由扩增的DNA靶的存在引起的探针的切割。