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    Multiply-substituted protease variants with altered net charge for use in detergents
    13.
    发明授权
    Multiply-substituted protease variants with altered net charge for use in detergents 有权

    公开(公告)号：US06673590B1

    公开(公告)日：2004-01-06

    申请号：US09177353

    申请日：1998-10-23

    申请人： Ayrookaran .J. Poulose ,  Volker Schellenberger ,  James T. Kellis, Jr. ,  Christian Paech ,  Joanne Nadherny ,  Donald P. Naki ,  Katherine D. Collier ,  Robert M. Caldwell

    发明人： Ayrookaran .J. Poulose ,  Volker Schellenberger ,  James T. Kellis, Jr. ,  Christian Paech ,  Joanne Nadherny ,  Donald P. Naki ,  Katherine D. Collier ,  Robert M. Caldwell

    IPC分类号： C12N954

    CPC分类号： C12N9/54 ,  A23G4/123 ,  A23K20/189 ,  A61K8/66 ,  A61K2800/86 ,  A61Q11/00 ,  A61Q19/00 ,  C11D3/386 ,  C11D3/38609 ,  C11D3/38618 ,  C11D3/3907 ,  C11D3/3945 ,  C12Y304/00 ,  C12Y304/21062

    摘要： Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Protease variants are provided that contain substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and thus the protease variant is more effective in a low detergent concentration system than a precursor protease. Also provided are protease variants containing substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more positive or less negative compared to a precursor protease and thus the protease variant is more effective in a high detergent concentration system than a precursor protease. Protease variants are provided that contain substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and thus the protease variant is more effective in a medium detergent concentration system than a precursor protease. Also provided are protease variants containing substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more positive or less negative compared to a precursor protease and thus the protease variant is more effective in a medium detergent concentration system than a precursor protease. Further provided is a method of producing a protease variant that is more effective in a low detergent concentration system, medium detergent concentration system and high detergent concentration system than a precursor protease.

    Enzymatic modification of the surface of a polyester fiber or article
    17.
    发明授权
    Enzymatic modification of the surface of a polyester fiber or article 有权
    聚酯纤维或制品的表面的酶改性

    公开(公告)号：US06254645B1

    公开(公告)日：2001-07-03

    申请号：US09435083

    申请日：1999-11-05

    申请人： James T. Kellis, Jr. ,  Ayrookaran J. Poulose ,  Mee-Young Yoon

    发明人： James T. Kellis, Jr. ,  Ayrookaran J. Poulose ,  Mee-Young Yoon

    IPC分类号： C09B6700

    CPC分类号： D06P3/522 ,  D06M16/003 ,  D06M2101/32 ,  D06M2200/00 ,  D06P1/0004 ,  D06P5/22 ,  Y10S8/04

    摘要： A method is provided for improving the uptake of a cationic compound onto a polyester article starting material, comprising the steps of: (a) obtaining a polyesterase enzyme; (b) contacting said polyesterase enzyme with said polyester article starting material under conditions and for a time suitable for said polyesterase to produce surface modification of said polyester article starting material and produce a surface modified polyester; (c) contacting said modified polyester article, subsequently or simultaneously with said step (b) with a cationic compound whereby adherence of said cationic compound to said modified polyester is increased compared to said polyester starting material. Also disclosed is a method for increasing the hydrophilicity of a polyester to improve fabric characteristics such as stain resistance, wettability and/or dyeability.

    摘要翻译： 提供了一种改善阳离子化合物在聚酯制品原料上的吸收的方法，包括以下步骤：（a）获得聚酯酶; （b）使所述聚酯酶与所述聚酯制品起始材料在适于所述聚酯酶的条件下进行接触以产生所述聚酯制品原料的表面改性并制备表面改性聚酯; （c）随后或与所述步骤（b）同时地将所述改性聚酯制品与阳离子化合物接触，由此所述阳离子化合物与所述改性聚酯的粘合比所述聚酯起始材料增加。 还公开了提高聚酯的亲水性以提高织物特性如耐污染性，润湿性和/或染色性的方法。