公开(公告)号：US08460916B2

公开(公告)日：2013-06-11

申请号：US13540064

申请日：2012-07-02

申请人： Luis G. Cascao-Pereira ,  Claudine Chang ,  Clement Choy ,  James T. Kellis, Jr. ,  Brian E. Jones ,  Melodie Estabrook ,  Marc Koklman ,  Chris Leeflang ,  Casper Vroeman ,  Walter Weyler

发明人： Luis G. Cascao-Pereira ,  Claudine Chang ,  Clement Choy ,  James T. Kellis, Jr. ,  Brian E. Jones ,  Melodie Estabrook ,  Marc Koklman ,  Chris Leeflang ,  Casper Vroeman ,  Walter Weyler

IPC分类号： C12P21/06 ,  C12N9/00 ,  C12N9/28 ,  C12N1/20 ,  C12N15/00 ,  C07K17/00 ,  C07H21/04

CPC分类号： C12N9/2417 ,  A21D8/042 ,  A23K20/189 ,  A23K50/75 ,  A23L29/06

摘要： Described are variants (mutants) of a parent alpha-amylase having alpha-amylase activity and exhibiting altered properties relative to the parent alpha-amylase, and methods of use, thereof.

公开(公告)号：US08236545B2

公开(公告)日：2012-08-07

申请号：US12365646

申请日：2009-02-04

申请人： Luis G. Cascao-Pereira ,  Claudine Chang ,  Clement Choy ,  James T. Kellis, Jr. ,  Brian E Jones ,  Melodie Estabrook ,  Marc Kolkman ,  Chris Leeflang ,  Casper Vroemen ,  Walter Weyler

发明人： Luis G. Cascao-Pereira ,  Claudine Chang ,  Clement Choy ,  James T. Kellis, Jr. ,  Brian E Jones ,  Melodie Estabrook ,  Marc Kolkman ,  Chris Leeflang ,  Casper Vroemen ,  Walter Weyler

IPC分类号： C12P21/06 ,  C12N9/00 ,  C12N9/28 ,  C11D3/386 ,  C07K1/00 ,  C07H21/04

CPC分类号： C12N9/2417 ,  A21D8/042 ,  A23K20/189 ,  A23K50/75 ,  A23L29/06

摘要： Described are variants (mutants) of a parent alpha-amylase having alpha-amylase activity and exhibiting altered properties relative to the parent alpha-amylase, and methods of use, thereof.

摘要翻译： 描述了具有α-淀粉酶活性并且相对于母体α-淀粉酶具有改变的性质的亲本α-淀粉酶的变体（突变体）及其使用方法。

公开(公告)号：US09856466B2

公开(公告)日：2018-01-02

申请号：US14115801

申请日：2012-05-04

申请人： Neelam S. Amin ,  Katherine Augustyn ,  Joshua R. Basler ,  Luis G. Cascao-Pereira ,  Katherine D. Collier ,  Edward M. Concar ,  David A. Estell ,  James T. Kellis, Jr. ,  Euan John Magennis ,  Alexander Pisarchik ,  Ayrookaran J. Poulose ,  Philip Frank Souter ,  Glenn Steven Ward ,  Jian Yao

发明人： Neelam S. Amin ,  Katherine Augustyn ,  Joshua R. Basler ,  Luis G. Cascao-Pereira ,  Katherine D. Collier ,  Edward M. Concar ,  David A. Estell ,  James T. Kellis, Jr. ,  Euan John Magennis ,  Alexander Pisarchik ,  Ayrookaran J. Poulose ,  Philip Frank Souter ,  Glenn Steven Ward ,  Jian Yao

IPC分类号： C12N9/54 ,  C11D3/386

CPC分类号： C12N9/54 ,  C11D3/386

摘要： The present invention provides serine protease variants, more specifically subtilisin variants produced there from. Specifically, the present invention provides serine protease variants, more specifically subtilisin variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants, more specifically subtilisin variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants, more specifically subtilisin variants.

公开(公告)号：US08183024B2

公开(公告)日：2012-05-22

申请号：US12616106

申请日：2009-11-10

申请人： Luis G. Cascao-Pereira ,  David A. Estell ,  Frits Goedegebuur ,  James T. Kellis, Jr. ,  Ayrookaran J. Poulise

发明人： Luis G. Cascao-Pereira ,  David A. Estell ,  Frits Goedegebuur ,  James T. Kellis, Jr. ,  Ayrookaran J. Poulise

IPC分类号： C12N9/48

CPC分类号： C11D3/386 ,  C11D3/38681 ,  C11D17/0086

摘要： The present invention provides a subtilisin variant that is particularly well suited to cleaning applications. In particular, the present invention provides a Bacillus sp. subtilisin variant and cleaning compositions comprising this variant.

摘要翻译： 本发明提供了特别适合于清洁应用的枯草杆菌蛋白酶变体。 特别地，本发明提供芽孢杆菌属 枯草溶菌素变体和包含该变体的清洁组合物。

公开(公告)号：US08753861B2

公开(公告)日：2014-06-17

申请号：US13128854

申请日：2009-11-10

申请人： Luis G. Cascao-Pereira ,  David A. Estell ,  James T. Kellis, Jr.

发明人： Luis G. Cascao-Pereira ,  David A. Estell ,  James T. Kellis, Jr.

IPC分类号： C12N9/54 ,  C12N1/21

CPC分类号： C12N9/54 ,  C11D3/386 ,  C11D3/38609 ,  C11D3/38618 ,  C11D3/38663 ,  C11D3/38681 ,  C12N15/52 ,  C12Y304/21062

摘要： The present invention provides engineered protease variants. In particular, the protease variants comprise combinable mutations at selected surface positions that affect the charge and/or hydrophobicity of the enzyme to enhance at least one desired property of the resulting variant enzyme in a chosen application. Compositions comprising the protease variants, and methods for using the same are also provided.

摘要翻译： 本发明提供工程蛋白酶变体。 特别地，蛋白酶变体在选定的表面位置包含影响酶的电荷和/或疏水性的可组合突变，以在所选择的应用中增强所得变体酶的至少一种所需性质。 还提供了包含蛋白酶变体的组合物及其使用方法。

公开(公告)号：US08916370B2

公开(公告)日：2014-12-23

申请号：US13436612

申请日：2012-03-30

申请人： Richard R. Bott ,  Marguerite A. Cervin ,  James T. Kellis, Jr. ,  Joseph C. McAuliffe ,  Andrei Miasnikov ,  Caroline M. Peres ,  Christopher Lee Rife ,  Derek H. Wells ,  Walter Weyler ,  Gregory M. Whited

发明人： Richard R. Bott ,  Marguerite A. Cervin ,  James T. Kellis, Jr. ,  Joseph C. McAuliffe ,  Andrei Miasnikov ,  Caroline M. Peres ,  Christopher Lee Rife ,  Derek H. Wells ,  Walter Weyler ,  Gregory M. Whited

IPC分类号： C12N9/88 ,  C12P21/06 ,  C12N15/00 ,  C12P5/02 ,  C12N1/20 ,  C12N1/00 ,  C07H21/04 ,  C07K1/00 ,  C12P5/00

CPC分类号： C12N9/88 ,  C12P5/007 ,  C12Y402/03027

摘要： The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.

摘要翻译： 本发明提供包含至少一种具有改善的催化活性和/或溶解度的异戊二烯合酶的方法和组合物。 特别地，本发明提供用于在微生物宿主细胞中增加异戊二烯生产的变体植物异戊二烯合酶。 本发明的生物合成生产的异戊二烯可用于制造橡胶和弹性体。

公开(公告)号：US08173410B2

公开(公告)日：2012-05-08

申请号：US12429143

申请日：2009-04-23

申请人： Richard R. Bott ,  Marguerite A. Cervin ,  James T. Kellis, Jr. ,  Joseph C. McAuliffe ,  Andrei Miasnikov ,  Caroline M. Peres ,  Christopher Lee Rife ,  Derek H. Wells ,  Walter Weyler ,  Gregory M. Whited

发明人： Richard R. Bott ,  Marguerite A. Cervin ,  James T. Kellis, Jr. ,  Joseph C. McAuliffe ,  Andrei Miasnikov ,  Caroline M. Peres ,  Christopher Lee Rife ,  Derek H. Wells ,  Walter Weyler ,  Gregory M. Whited

IPC分类号： C12N9/88 ,  C12N15/09 ,  C12N15/00 ,  C12N1/20 ,  C12P5/02 ,  C07H21/04 ,  C07K1/00

CPC分类号： C12N9/88 ,  C12P5/007 ,  C12Y402/03027

摘要： The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.

摘要翻译： 本发明提供包含至少一种具有改善的催化活性和/或溶解度的异戊二烯合酶的方法和组合物。 特别地，本发明提供用于在微生物宿主细胞中增加异戊二烯生产的变体植物异戊二烯合酶。 本发明的生物合成生产的异戊二烯可用于制造橡胶和弹性体。

公开(公告)号：US08206966B2

公开(公告)日：2012-06-26

申请号：US12263886

申请日：2008-11-03

申请人： Luis G. Cascāo-Pereira ,  James T. Kellis, Jr. ,  Bradley A. Paulson ,  Scott D. Power ,  Sandra W. Ramer ,  Vivek Sharma ,  Andrew Shaw ,  Jayarama K. Shetty ,  Donald E. Ward

发明人： Luis G. Cascāo-Pereira ,  James T. Kellis, Jr. ,  Bradley A. Paulson ,  Scott D. Power ,  Sandra W. Ramer ,  Vivek Sharma ,  Andrew Shaw ,  Jayarama K. Shetty ,  Donald E. Ward

IPC分类号： C12N9/26 ,  C12N9/28 ,  C12N9/14 ,  C12N9/16 ,  C12N1/20 ,  C12N15/00 ,  C12P19/14 ,  C12P21/06 ,  C12P19/34 ,  C07H21/04 ,  C12C11/00 ,  A21D2/16 ,  C07K1/00

CPC分类号： C11D3/38681 ,  C11D3/386 ,  C12N9/2417 ,  D06M16/003

摘要： Disclosed are compositions comprising variants of alpha-amylase that have alpha-amylase activity and which exhibit altered properties relative to a parent AmyS-like alpha-amylase from which they are derived. The compositions comprise an additional enzyme such as a phytase. Also disclosed are methods of using the compositions, and kits related thereto.

摘要翻译： 公开了包含α-淀粉酶变体的组合物，其具有α-淀粉酶活性，并且相对于其来源的亲本AmyS样α-淀粉酶表现出改变的性质。 组合物包含另外的酶如植酸酶。 还公开了使用组合物的方法和与其相关的试剂盒。

公开(公告)号：US08785171B2

公开(公告)日：2014-07-22

申请号：US12963681

申请日：2010-12-09

申请人： Philip Frank Souter ,  Glenn Steven Ward ,  Viktor Yuryevich Alekseyev ,  Joshua Roy Basler ,  Luis Gustavo Cascão-Pereira ,  David A. Estell ,  Ayrookaran J. Poulose ,  James T. Kellis, Jr. ,  Alexander Pisarchik ,  Daniel Esteban Torres-Pazmino

发明人： Philip Frank Souter ,  Glenn Steven Ward ,  Viktor Yuryevich Alekseyev ,  Joshua Roy Basler ,  Luis Gustavo Cascão-Pereira ,  David A. Estell ,  Ayrookaran J. Poulose ,  James T. Kellis, Jr. ,  Alexander Pisarchik ,  Daniel Esteban Torres-Pazmino

IPC分类号： C12N9/48

CPC分类号： C11D3/38618 ,  C11D3/38609 ,  C11D3/38681 ,  C12N9/50

摘要： This invention relates to fabric and home care products comprising one or more cold water proteases and processes for making and using such products. Such compositions provide improved cleaning and freshness. Such cold water proteases may be derived from parent enzymes, including BPN′ subtilisin and subtilisin derived from Bacillus lentus, by substitution, insertion and/or deletion of one or more of the parent enzymes' amino acids.

摘要翻译： 本发明涉及包含一种或多种冷水蛋白酶的织物和家庭护理产品以及用于制造和使用这些产品的方法。 这样的组合物提供改善的清洁和新鲜度。 这样的冷水蛋白酶可以通过取代，插入和/或缺失一种或多种亲本氨基酸从母体酶衍生，包括BPN'枯草杆菌蛋白酶和源自迟缓芽孢杆菌的枯草杆菌蛋白酶。

公开(公告)号：US06254645B1

公开(公告)日：2001-07-03

申请号：US09435083

申请日：1999-11-05

申请人： James T. Kellis, Jr. ,  Ayrookaran J. Poulose ,  Mee-Young Yoon

发明人： James T. Kellis, Jr. ,  Ayrookaran J. Poulose ,  Mee-Young Yoon

IPC分类号： C09B6700

CPC分类号： D06P3/522 ,  D06M16/003 ,  D06M2101/32 ,  D06M2200/00 ,  D06P1/0004 ,  D06P5/22 ,  Y10S8/04

摘要： A method is provided for improving the uptake of a cationic compound onto a polyester article starting material, comprising the steps of: (a) obtaining a polyesterase enzyme; (b) contacting said polyesterase enzyme with said polyester article starting material under conditions and for a time suitable for said polyesterase to produce surface modification of said polyester article starting material and produce a surface modified polyester; (c) contacting said modified polyester article, subsequently or simultaneously with said step (b) with a cationic compound whereby adherence of said cationic compound to said modified polyester is increased compared to said polyester starting material. Also disclosed is a method for increasing the hydrophilicity of a polyester to improve fabric characteristics such as stain resistance, wettability and/or dyeability.

摘要翻译： 提供了一种改善阳离子化合物在聚酯制品原料上的吸收的方法，包括以下步骤：（a）获得聚酯酶; （b）使所述聚酯酶与所述聚酯制品起始材料在适于所述聚酯酶的条件下进行接触以产生所述聚酯制品原料的表面改性并制备表面改性聚酯; （c）随后或与所述步骤（b）同时地将所述改性聚酯制品与阳离子化合物接触，由此所述阳离子化合物与所述改性聚酯的粘合比所述聚酯起始材料增加。 还公开了提高聚酯的亲水性以提高织物特性如耐污染性，润湿性和/或染色性的方法。