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    ALPHA-AMYLASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME
    15.
    发明申请
    ALPHA-AMYLASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME 审中-公开

    公开(公告)号：US20180208916A1

    公开(公告)日：2018-07-26

    申请号：US15746712

    申请日：2016-07-20

    申请人： NOVOZYMES A/S

    发明人： Carsten Andersen ,  Jameel Shihadeh

    IPC分类号： C12N9/28 ,  C12P19/14 ,  C12N9/54 ,  C12P19/02 ,  C12N9/34

    CPC分类号： C12N9/2417 ,  C12N9/242 ,  C12N9/2428 ,  C12N9/54 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01001

    摘要： The present invention relates to an alpha-amylase variant, comprising a substitution at one or more positions corresponding to positions 59, 129, 177, 179, 208, 212, 220, 224, 254, and 284 of the polypeptide of SEQ ID NO: 1, wherein the variant has at least at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the mature polypeptide of SEQ ID NO: 1, and wherein the variant comprises at least one of the following combination of substitutions: V59A+E129V+K177L+R179E+H208Y+K220P+N224L+Q254S; V59A+E129V+K177L+R179E+Q254S+M284V; or V59A+E129V+K177L+R179E+V212T+Q254S+M284V (using SEQ ID NO: 1 for numbering); and wherein the variants have alpha-amylase activity.

    POLYPEPTIDES HAVING ALPHA AMYLASE ACTIVITY
    17.
    发明申请
    POLYPEPTIDES HAVING ALPHA AMYLASE ACTIVITY 审中-公开
    具有ALPHA AMYLASE活性的多肽

    公开(公告)号：US20170015950A1

    公开(公告)日：2017-01-19

    申请号：US15301195

    申请日：2015-04-01

    申请人： NOVOZYMES A/S

    发明人： Carsten Andersen ,  Iben Damager ,  Astrid Munch

    IPC分类号： C11D3/386 ,  C12N9/26

    CPC分类号： C11D3/386 ,  C12N9/2414 ,  C12N9/2417 ,  C12Y302/01001

    摘要： The present invention relates to polypeptides having alpha-amylase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

    摘要翻译： 本发明涉及具有α-淀粉酶活性的多肽和编码该多肽的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。

    Phytase Variants
    18.
    发明申请
    Phytase Variants 审中-公开
    植酸酶变体

    公开(公告)号：US20160362666A1

    公开(公告)日：2016-12-15

    申请号：US15249856

    申请日：2016-08-29

    申请人： Novozymes A/S

    发明人： Leonardo De Maria ,  Carsten Andersen ,  Lars Kobberoee Skov ,  Mikael Blom Soerensen

    IPC分类号： C12N9/16 ,  A23K20/189

    CPC分类号： C12N9/16 ,  A23K20/189 ,  C12Y301/03

    摘要： The present invention relates to a phytase which has at least 74% identity to a phytase derived from Citrobacter braakii and comprises at least one alteration as compared to this phytase. These phytase variants have amended, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensibility, and/or an amended glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.

    摘要翻译： 本发明涉及一种植酸酶，其与来自柠檬酸杆菌的植酸酶具有至少74％的同一性，并且与该植酸酶相比包含至少一个改变。 这些植酸酶变体具有修饰，优选改进的性质，例如热稳定性，温度曲线，pH曲线，比活性，动物饲料中的性能，降低的蛋白酶敏感性和/或修饰的糖基化模式。 本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。