利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

70 条记录 (耗时 0.042 秒)

    Lysine-specific Porphyromonas gingivalis proteinase
    11.
    发明授权
    Lysine-specific Porphyromonas gingivalis proteinase 失效
    赖氨酸特异性牙龈卟啉单胞菌牙龈蛋白酶

    公开(公告)号：US5475097A

    公开(公告)日：1995-12-12

    申请号：US141324

    申请日：1993-10-21

    申请人： James Travis ,  J. Stanislaw Potempa ,  Philip J. Barr ,  Nadine Pavloff ,  Robert N. Pike

    发明人： James Travis ,  J. Stanislaw Potempa ,  Philip J. Barr ,  Nadine Pavloff ,  Robert N. Pike

    IPC分类号： C12N9/52 ,  C12N15/51

    CPC分类号： C12N9/52

    摘要： Provide herein is a substantially pure Lys-gingipain complex preparation, Lys-gingipain being characterized as having an apparent molecular mass of 105 kDa as estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, where sample is prepared without boiling, said Lys-gingipain having amidolytic and proteolytic activity for cleavage after lysine residues and having no amidolytic and/or proteolytic activity for cleavage after arginine residues, wherein the amidolytic and/or proteolytic activity is inhibited by TLCK, cysteine protease group-specific inhibitors including iodoacetamide and iodoacetic acid, wherein the amidolytic and/or proteolytic activity of said Lys-gingipain is not sensitive to inhibition by leupeptin, antipain, trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane, serine protease group-specific inhibitors including diisopropylfluorophosphate and phenylmethyl sulfonylfluoride, and antibodies specific for the Lys-gingipain protein complex and its catalytic component, methods for preparation. As specifically exemplified, a Lys-gingipain protein complex is purified from Porphyromonas gingivalis H66, and the 60 kDa catalytic component of the Lys-gingipain protein complex has an amino acid sequence as given in SEQ ID NO: 14 from amino acid 1 through amino acid 509. Also provided are nucleic acid sequences encoding this catalytic protein. The nucleotide coding sequence of the 60 kDa catalytic component of the Lys-gingipain protein complex is given in SEQ ID NO:13, from nucleotide 1336 through nucleotide 2863. The Lys-gingipain complex also comprises a hemagglutinin component identified by an N-terminal amino acid sequence as given in SEQ ID NO:14, amino acids 510-714.

    摘要翻译： 本文提供了一种基本上纯的赖氨酸蛋白糊精复合物制剂，其特征在于具有105kDa的表观分子量，通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳测定，其中样品在不沸腾的情况下制备，所述Lys-盖蛋白具有酰胺分解和 在赖氨酸残基之后切割的蛋白水解活性，并且在精氨酸残基之后没有用于切割的酰胺解和/或蛋白水解活性，其中所述酰胺分解和/或蛋白水解活性被TLCK抑制，包括碘乙酰胺和碘乙酸的半胱氨酸蛋白酶组特异性抑制剂，其中酰胺分解 所述Lys-gingipain的蛋白水解和/或蛋白水解活性对亮抑酶肽，抗胰蛋白酶，反式环氧琥珀酰基-L-亮氨酰氨基 - （4-胍基）丁烷，丝氨酸蛋白酶组特异性抑制剂，包括二异丙基氟磷酸酯和苯基甲基磺酰氟的抑制和抗体特异性 用于Lys-gingipain蛋白复合物及其催化 组分，制备方法。 如具体示例的那样，从牙龈卟啉单胞菌H66中纯化Lys-gingipain蛋白复合物，Lys-gingipain蛋白复合物的60kDa催化组分具有SEQ ID NO：14中从氨基酸1到氨基酸所给出的氨基酸序列 还提供了编码该催化蛋白的核酸序列。 Lys-gingipain蛋白质复合物的60kDa催化组分的核苷酸编码序列在SEQ ID NO：13中由核苷酸1336至核苷酸2863给出。Lys-gingipain复合物还包含由N-末端氨基酸鉴定的血凝素成分 酸序列，如SEQ ID NO：14所示，氨基酸510-714。

    Antenna Near-Field Probe Station Scanner
    13.
    发明申请
    Antenna Near-Field Probe Station Scanner 审中-公开
    天线近场探测台扫描仪

    公开(公告)号：US20110032253A1

    公开(公告)日：2011-02-10

    申请号：US12857004

    申请日：2010-08-16

    申请人： Afroz J. Zaman ,  Richard Q. Lee ,  William G. Darby ,  Philip J. Barr ,  Kevin M. Lambert ,  Felix A. Miranda

    发明人： Afroz J. Zaman ,  Richard Q. Lee ,  William G. Darby ,  Philip J. Barr ,  Kevin M. Lambert ,  Felix A. Miranda

    IPC分类号： G06T17/00

    CPC分类号： G01R29/105 ,  H01Q3/08

    摘要： A miniaturized antenna system is characterized non-destructively through the use of a scanner that measures its near-field radiated power performance. When taking measurements, the scanner can be moved linearly along the x, y and z axis, as well as rotationally relative to the antenna. The data obtained from the characterization are processed to determine the far-field properties of the system and to optimize the system. Each antenna is excited using a probe station system while a scanning probe scans the space above the antenna to measure the near field signals. Upon completion of the scan, the near-field patterns are transformed into far-field patterns. Along with taking data, this system also allows for extensive graphing and analysis of both the near-field and far-field data. The details of the probe station as well as the procedures for setting up a test, conducting a test, and analyzing the resulting data are also described.

    摘要翻译： 小型化天线系统通过使用测量其近场辐射功率性能的扫描仪非破坏性地表征。 当进行测量时，扫描仪可以沿x，y和z轴线性移动，以及相对于天线旋转。 处理从表征获得的数据以确定系统的远场属性并优化系统。 每个天线使用探测台系统激励，而扫描探针扫描天线上方的空间来测量近场信号。 扫描完成后，近场图案被转换为远场图案。 随着采集数据，该系统还可以对近场和远场数据进行广泛的绘图和分析。 还描述了探测台的细节以及建立测试，进行测试和分析结果数据的步骤。

    Expression and processing of amino terminus acetylated FGF's in yeast
    16.
    发明授权
    Expression and processing of amino terminus acetylated FGF's in yeast 失效
    酵母中氨基末端乙酰化FGF的表达和处理

    公开(公告)号：US5656458A

    公开(公告)日：1997-08-12

    申请号：US268656

    申请日：1994-06-29

    申请人： Philip J. Barr

    发明人： Philip J. Barr

    IPC分类号： C07K14/50 ,  C12N15/09 ,  C12N15/81

    CPC分类号： C07K14/503 ,  C07K14/501

    摘要： Methods and compositions are provided for producing, in yeast, basic and acidic fibroblast growth factors (FGF's) that are at least partially acetylated at their amino-termini. DNA constructs containing genes coding for the FGF polypeptides under transcriptional control of a regulatable promoter are expressed in transformed host yeast cells to achieve high yields of the processed FGF's.

    摘要翻译： 提供了用于在酵母中生产在其氨基末端至少部分乙酰化的碱性和酸性成纤维细胞生长因子（FGF's）的方法和组合物。 在转化的宿主酵母细胞中表达含有编码可调节启动子转录控制下的FGF多肽的基因的DNA构建体，以获得高产量的加工FGF。

    NOVEL BAK BINDING PROTEIN, DNA ENCODING THE PROTEIN, AND METHODS OF USE THEREOF
    20.
    发明申请
    NOVEL BAK BINDING PROTEIN, DNA ENCODING THE PROTEIN, AND METHODS OF USE THEREOF 审中-公开
    新蛋白结合蛋白，编码蛋白的DNA及其使用方法

    公开(公告)号：US20120142099A1

    公开(公告)日：2012-06-07

    申请号：US13009228

    申请日：2011-01-19

    申请人： Michael C. Kiefer ,  Paul A. Fitzpatrick ,  Helen L. Gibson ,  Philip J. Barr

    发明人： Michael C. Kiefer ,  Paul A. Fitzpatrick ,  Helen L. Gibson ,  Philip J. Barr

    IPC分类号： C12N5/02

    CPC分类号： C07K14/47 ,  A61K38/00 ,  A61K48/00 ,  C07K2319/00 ,  G01N2333/47 ,  G01N2500/02 ,  G01N2510/00

    摘要： The present invention provides polynucleotide sequences (bbp) encoding a Bak Binding Protein (BBP) and fragments thereof that bind to Bak. The invention also provides a BBP which binds to Bak. The invention also provides recombinant host cells containing polynucleotides encoding BBP. The invention further provides antibodies that specifically bind to BBP. The invention further provides methods for detecting agents such as drugs that alter the binding of a BBP with a Bak protein. The invention further provides methods for detecting the presence of bbp or BBP in a biological sample, and further provides methods for modulating the levels of BBP in a cell. This invention additionally encompasses novel peptides, designated the “BBP Binding Domains” and the respective nucleotides, designated “bbpbd-1” and “bbpbd-2” which are involved in the interaction between Bak and BBP.

    摘要翻译： 本发明提供编码Bak结合蛋白（BBP）的多核苷酸序列（bbp）及其与Bak结合的片段。 本发明还提供了结合Bak的BBP。 本发明还提供含有编码BBP的多核苷酸的重组宿主细胞。 本发明还提供了特异性结合BBP的抗体。 本发明还提供了用于检测诸如改变BBP与Bak蛋白的结合的药物的药物的方法。 本发明还提供了用于检测生物样品中bbp或BBP的存在的方法，并且还提供了调节细胞中BBP水平的方法。 本发明另外包括称为“BBP结合域”的新肽，以及涉及Bak和BBP之间的相互作用的“bbpbd-1”和“bbpbd-2”的相应核苷酸。