公开(公告)号：US20230235274A1

公开(公告)日：2023-07-27

申请号：US18012433

申请日：2021-06-25

Applicant: QVELLA CORPORATION

Inventor： Samad TALEBPOUR ,  Anna KHIMCHENKO ,  Maryam ASADISHEKARI ,  Stephen Wesley LEONARD

IPC: C12N1/20 ,  G02B21/36 ,  G06V20/69 ,  G06T5/50 ,  G06T5/00 ,  G06V10/774 ,  G06V10/82 ,  G06V10/147

CPC classification number: C12N1/20 ,  G02B21/367 ,  G06V20/693 ,  G06V20/698 ,  G06T5/50 ,  G06T5/002 ,  G06V10/774 ,  G06V10/82 ,  G06V10/147 ,  G06T2207/10056 ,  G06T2207/30024 ,  G06T2207/20212 ,  G06T2207/20081 ,  G06T2207/20084

Abstract: Systems and methods are provided for classifying microbial cells according to morphological features of microcolonies. A dark-field objective is employed to acquire a dark-field image of a microcolony during a microcolony growth phase that is characterized by phenotypic expression of microcolony morphological features which evolve with time and are differentiated among classes of microbial cell types. The dark-field image is processed to classify the microcolony according to two or more microbial cell types, such as Gram status and/or speciation. The dark-field objective may have a numerical aperture selected to facilitate the imaging of microcolony morphological features, residing, for example, between 0.15 and 0.35. A set of dark-field images of a microcolony may be collected during the microcolony growth phase and processed to classify the microcolony. Classification may be performed according to a temporal ordering of the dark-field images, for example, using a recurrent neural network.

公开(公告)号：US20200206739A1

公开(公告)日：2020-07-02

申请号：US16818095

申请日：2020-03-13

Applicant: QVELLA CORPORATION

Inventor： Robert MAASKANT ,  Sanjesh YASOTHARAN ,  Samad TALEBPOUR ,  Stephen W. LEONARD ,  Cyrus ETEMAD-MOGHADAM ,  Alexander ZAHN

IPC: B01L3/00 ,  F16K99/00 ,  C12N15/10 ,  B01D21/26 ,  B04B13/00 ,  B04B5/04 ,  B01L7/00 ,  F16K7/00 ,  B01D17/02 ,  B01D19/00

Abstract: Systems ,methods and devices are provided for the automated centrifugal processing of samples. In some embodiments, an integrated fluidic processing cartridge is provided, in which a centrifugation chamber is fluidically interfaced, through a lateral surface thereof, with a microfluidic device, and wherein the integrated fluidic processing cartridge is configured to be inserted into a centrifuge for centrifugation. A cartridge interfacing assembly may be employed to interface with the integrated fluidic processing cartridge for performing various fluidic processing steps, such as controlling the flow of fluids into and out of the centrifugation chamber, and controlling the flow of fluids into the microfluidic device, and optionally for the further fluidic processing of fluids extracted to the micro-fluidic device. The integrated fluidic processing cartridge may include a supernatant chamber the extraction of a supernatant thereto, and a dilutent chamber for diluting a suspension collected in the centrifugation chamber.

公开(公告)号：US20170211128A1

公开(公告)日：2017-07-27

申请号：US15401291

申请日：2017-01-09

Applicant: QVELLA CORPORATION

Inventor： Samad TALEBPOUR ,  Aye Aye KHINE ,  Robert MAASKANT ,  Tino ALAVIE

IPC: C12Q1/68 ,  C12N13/00 ,  C12N1/06 ,  B01L3/00 ,  B01L7/00

CPC classification number: C12Q1/6806 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/502753 ,  B01L7/52 ,  B01L2200/0631 ,  B01L2200/143 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0809 ,  B01L2300/0858 ,  B01L2300/087 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/14 ,  B01L2300/1833 ,  B01L2400/0694 ,  C12M35/02 ,  C12M47/06 ,  C12N1/066 ,  C12N1/08 ,  C12N13/00 ,  C12Q1/686 ,  C12Q3/00

Abstract: Devices and methods are provided for electrically lysing cells and releasing macromolecules from the cells. A microfluidic device is provided that includes a planar channel having a thickness on a submillimeter scale, and including electrodes on its upper and lower inner surfaces. After filling the channel with a liquid, such that the channel contains cells within the liquid, a series of voltage pulses of alternating polarity are applied between the channel electrodes, where the amplitude of the voltage pulses and a pulsewidth of the voltage pulses are effective for causing irreversible electroporation of the cells. The channel is configured to possess thermal properties such that the application of the voltage produces a rapid temperature rise as a result of Joule heating for releasing the macromolecules from the electroplated cells. The channel may also include an internal filter for capturing and concentrating the cells prior to electrical processing.