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11.发明申请ZYMOMONAS WITH IMPROVED ETHANOL PRODUCTION IN MEDIUM CONTAINING CONCENTRATED SUGARS AND ACETATE 有权含有浓缩糖和乙酸的中等乙醇生产的ZYMOMONASAS公开(公告)号：US20090221078A1
公开(公告)日：2009-09-03
申请号：US12261166
申请日：2008-10-30
申请人： Perry G. Caimi , Yat-Chen Chou , Mary Ann Franden , Kyle Knoke , Luan Tao , Paul V. Viitanen , Min Zhang , Yuying Zhang
发明人： Perry G. Caimi , Yat-Chen Chou , Mary Ann Franden , Kyle Knoke , Luan Tao , Paul V. Viitanen , Min Zhang , Yuying Zhang
CPC分类号： C12N15/52 , C12N9/1022 , C12N9/1205 , C12N9/92 , C12N15/74 , C12P7/065 , Y02E50/16 , Y02E50/17
摘要： Through screening of a Zymomonas mutant library the himA gene was found to be involved in the inhibitory effect of acetate on Zymomonas performance. Xylose-utilizing Zymomonas further engineered to reduce activity of the himA gene were found to have increased ethanol production in comparison to a parental strain, when cultured in medium comprising xylose and acetate.
摘要翻译： 通过筛选发酵单胞菌突变文库，发现himA基因参与乙酸盐对发酵单胞菌性能的抑制作用。 与在亲本菌株相比，当在含有木糖和乙酸酯的培养基中培养时，发现进一步工程化以减少himA基因活性的木糖酶发酵菌具有增加的乙醇产量。
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12.发明申请ZYMOMONAS WITH IMPROVED ETHANOL PRODUCTION IN MEDIUM CONTAINING CONCENTRATED SUGARS AND ACETATE 有权含有浓缩糖和乙酸的中等乙醇生产的ZYMOMONASAS公开(公告)号：US20090203099A1
公开(公告)日：2009-08-13
申请号：US12261160
申请日：2008-10-30
申请人： Perry G. Caimi , Yat-Chen Chou , Mary Ann Franden , Kyle Knoke , Luan Tao , Paul V. Viitanen , Min Zhang , Yuying Zhang
发明人： Perry G. Caimi , Yat-Chen Chou , Mary Ann Franden , Kyle Knoke , Luan Tao , Paul V. Viitanen , Min Zhang , Yuying Zhang
IPC分类号： C12P7/06
摘要： Through screening of a Zymomonas mutant library the himA gene was found to be involved in the inhibitory effect of acetate on Zymomonas performance. Xylose-utilizing Zymomonas strains further engineered to reduce activity of the himA gene were found to have increased ethanol production in comparison to a parental strain, when cultured in mixed-sugars medium comprising xylose, and, in particular, in the presence of acetate.
摘要翻译： 通过筛选发酵单胞菌突变文库，发现himA基因参与乙酸盐对发酵单胞菌性能的抑制作用。 当与含有木糖的混合糖介质，特别是在乙酸盐存在下培养时，发现与亲本菌株相比，进一步工程化以减少himA基因活性的木糖酶发酵单胞菌菌株具有增加的乙醇产量。
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公开(公告)号：US07361811B2
公开(公告)日：2008-04-22
申请号：US10718311
申请日：2003-11-20
申请人： Knut Meyer , Paul V. Viitanen , Drew E. Van Dyk
发明人： Knut Meyer , Paul V. Viitanen , Drew E. Van Dyk
CPC分类号： C12N9/88 , C07K2319/00 , C12N15/8243
摘要： The invention relates to high-level production of pHBA in green plants using a unique expression cassette. The latter comprises a chorismate pyruvate lyase (CPL) coding sequence operably linked to a suitable promoter capable of driving protein expression in higher plants. Additionally, the CPL cassette comprises a sequence encoding a chloroplast transit peptide, its natural cleavage site, and a small portion of the transit peptide donor protein fused to the N-terminus of CPL. The chloroplast targeting sequence targets the foreign protein to the chloroplast compartment and aids in its uptake into the organelle. The cleavage site is unique to the transit peptide, and cleavage of the chimeric protein encoded by the cassette at this site releases a novel polypeptide that has full enzyme activity, comprising the mature CPL enzyme and a small portion of the transit peptide donor.
摘要翻译： 本发明涉及使用独特的表达盒在绿色植物中高水平生产pHBA。 后者包括可操作地连接到能够驱动高等植物中的蛋白质表达的合适的启动子的草酸丙酸裂解酶（CPL）编码序列。 此外，CPL盒包含编码叶绿体转运肽，其天然切割位点的序列和与CPL的N末端融合的转运肽供体蛋白的一小部分。 叶绿体靶向序列将外来蛋白质靶向叶绿体隔室，并有助于其吸收到细胞器中。 切割位点对于转运肽是独特的，并且在该位点处由盒编码的嵌合蛋白质的切割释放出具有完整酶活性的新型多肽，其包含成熟的CPL酶和少量的转运肽供体。
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14.发明授权Process for inducing cytochrome P-450 enzymes in streptomyces bacteria and determining the mutagenicity of chemicals 失效在链霉菌中诱导细胞色素P-450酶的方法，并测定化学物质的致突变性
公开(公告)号：US5169755A
公开(公告)日：1992-12-08
申请号：US207630
申请日：1988-06-16
IPC分类号： C12N15/09 , C12N1/21 , C12N9/02 , C12N15/76 , C12Q1/02 , C12Q1/10 , C12Q1/26 , C12R1/465 , C12R1/545
CPC分类号： C12N9/0077 , C12N15/76 , C12Q1/025 , C12Q1/10 , C12Q1/26 , G01N2333/255 , G01N2333/36 , G01N2333/795 , G01N2333/80 , G01N2333/902 , G01N2333/90245 , Y10S435/886 , Y10S435/897
摘要： A process for inducting cytochrome P-450 enzyme production in bacteria of the genus Streptomyces using inducers such as soybean flour, genistein or genistin is described. Uses for the cytochrome P-450 enzymes produced are also discussed as is a process for using genetically engineered Streptomyces to determine the mutagenicity of chemicals.
摘要翻译： 描述了使用诱导剂如大豆粉，染料木素或染料木素诱导链霉菌属细菌中细胞色素P-450酶产生的方法。 还讨论了生产的细胞色素P-450酶的用途，是使用遗传工程化链霉菌来确定化学品的致突变性的方法。
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公开(公告)号：US08247208B2
公开(公告)日：2012-08-21
申请号：US12641642
申请日：2009-12-18
申请人： Perry G. Caimi , Mark Emptage , Xu Li , Paul V. Viitanen , Yat-Chen Chou , Mary Ann Franden , Min Zhang
发明人： Perry G. Caimi , Mark Emptage , Xu Li , Paul V. Viitanen , Yat-Chen Chou , Mary Ann Franden , Min Zhang
摘要： Strains of xylose utilizing Zymomonas with improved xylose utilization and ethanol production during fermentation in stress conditions were obtained using an adaptation method. The adaptation involved continuously growing xylose utilizing Zymomonas in media containing high sugars, acetic acid, ammonia, and ethanol.
摘要翻译： 使用适应方法获得使用具有改善的木糖利用的发酵单菌的木糖菌株和在胁迫条件下发酵期间的乙醇生产。 适应性涉及使用含有高糖，乙酸，氨和乙醇的培养基中的酵母单胞菌连续生长木糖。
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16.发明授权Xylitol synthesis mutant of xylose-utilizing zymomonas for ethanol production 有权用于乙醇生产的木糖利用酵母单体的木糖醇合成突变体公开(公告)号：US07741119B2
公开(公告)日：2010-06-22
申请号：US11862566
申请日：2007-09-27
申请人： Paul V. Viitanen , Yat-Chen Chou , Carol M. McCutchen , Min Zhang
发明人： Paul V. Viitanen , Yat-Chen Chou , Carol M. McCutchen , Min Zhang
摘要： A strain of xylose-utilizing Zymomonas was engineered with a genetic modification to the glucose-fructose oxidoreductase gene resulting in reduced expression of GFOR enzyme activity. The engineered strain exhibits reduced production of xylitol, a detrimental by-product of xylose metabolism. It also consumes more xylose and produces more ethanol during mixed sugar fermentation under process-relevant conditions.
摘要翻译： 用葡萄糖 - 果糖氧化还原酶基因的遗传修饰工程改造了利用木糖的酵母菌菌株，导致GFOR酶活性的降低。 工程化的菌株表现出木糖代谢产生不良副产物木糖醇的减少。 在过程相关条件下，在混合糖发酵过程中，它还消耗更多的木糖并产生更多的乙醇。
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公开(公告)号：US07056742B2
公开(公告)日：2006-06-06
申请号：US10462162
申请日：2003-06-16
申请人： Knut Meyer , Paul V. Viitanen , Dennis Flint
发明人： Knut Meyer , Paul V. Viitanen , Dennis Flint
CPC分类号： C12N15/8257
摘要： This invention relates to methods and materials to produce hydroquinone glucoside in genetically modified green plants and microorganisms.
摘要翻译： 本发明涉及在遗传修饰的绿色植物和微生物中产生氢醌葡糖苷的方法和材料。
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18.发明申请公开(公告)号：US20110014670A1
公开(公告)日：2011-01-20
申请号：US12641642
申请日：2009-12-18
申请人： PERRY G. CAIMI , Mark Emptage , Xu Li , Paul V. Viitanen , Yat-Chen Chou , Mary Ann Franden , Min Zhang
发明人： PERRY G. CAIMI , Mark Emptage , Xu Li , Paul V. Viitanen , Yat-Chen Chou , Mary Ann Franden , Min Zhang
摘要： Strains of xylose utilizing Zymomonas with improved xylose utilization and ethanol production during fermentation in stress conditions were obtained using an adaptation method. The adaptation involved continuously growing xylose utilizing Zymomonas in media containing high sugars, acetic acid, ammonia, and ethanol.
摘要翻译： 使用适应方法获得使用具有改善的木糖利用的发酵单菌的木糖菌株和在胁迫条件下发酵期间的乙醇生产。 适应性涉及使用含有高糖，乙酸，氨和乙醇的培养基中的酵母单胞菌连续生长木糖。
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19.发明申请XYLITOL SYNTHESIS MUTANT OF XYLOSE-UTILIZING ZYMOMONAS FOR ETHANOL PRODUCTION 有权用于乙醇生产的XYLOSE-ZYMOMONAS的XYLITOL合成突变体公开(公告)号：US20080286870A1
公开(公告)日：2008-11-20
申请号：US11862566
申请日：2007-09-27
申请人： Paul V. Viitanen , Yat-Chen Chou , Carol M. McCutchen , Min Zhang
发明人： Paul V. Viitanen , Yat-Chen Chou , Carol M. McCutchen , Min Zhang
摘要： A strain of xylose-utilizing Zymomonas was engineered with a genetic modification to the glucose-fructose oxidoreductase gene resulting in reduced expression of GFOR enzyme activity. The engineered strain exhibits reduced production of xylitol, a detrimental by-product of xylose metabolism. It also consumes more xylose and produces more ethanol during mixed sugar fermentation under process-relevant conditions.
摘要翻译： 用葡萄糖 - 果糖氧化还原酶基因的遗传修饰工程改造了利用木糖的酵母菌菌株，导致GFOR酶活性的降低。 工程化的菌株表现出木糖代谢产生不良副产物木糖醇的减少。 在过程相关条件下，在混合糖发酵过程中，它还消耗更多的木糖并产生更多的乙醇。
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20.发明授权Ethanol production using xylitol synthesis mutant of xylose-utilizing zymomonas 有权使用木糖利用酵母单体的木糖醇合成突变体的乙醇生产公开(公告)号：US07741084B2
公开(公告)日：2010-06-22
申请号：US11862736
申请日：2007-09-27
申请人： Paul V. Viitanen , Carol M. McCutchen , Mark Emptage , Perry G. Caimi , Min Zhang , Yat-Chen Chou
发明人： Paul V. Viitanen , Carol M. McCutchen , Mark Emptage , Perry G. Caimi , Min Zhang , Yat-Chen Chou
摘要： Production of ethanol using a strain of xylose-utilizing Zymomonas with a genetic modification of the glucose-fructose oxidoreductase gene was found to be improved due to greatly reduced production of xylitol, a detrimental by-product of xylose metabolism synthesized during fermentation.
摘要翻译： 发现使用具有葡萄糖 - 果糖氧化还原酶基因的遗传修饰的具有木糖利用的酵母菌菌株的乙醇的生产由于发酵期间合成的木糖代谢的有害副产物木糖醇的产生大大降低而得到改善。
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