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公开(公告)号：US07056742B2
公开(公告)日：2006-06-06
申请号：US10462162
申请日：2003-06-16
申请人： Knut Meyer , Paul V. Viitanen , Dennis Flint
发明人： Knut Meyer , Paul V. Viitanen , Dennis Flint
CPC分类号： C12N15/8257
摘要： This invention relates to methods and materials to produce hydroquinone glucoside in genetically modified green plants and microorganisms.
摘要翻译： 本发明涉及在遗传修饰的绿色植物和微生物中产生氢醌葡糖苷的方法和材料。
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公开(公告)号：US07361811B2
公开(公告)日：2008-04-22
申请号：US10718311
申请日：2003-11-20
申请人： Knut Meyer , Paul V. Viitanen , Drew E. Van Dyk
发明人： Knut Meyer , Paul V. Viitanen , Drew E. Van Dyk
CPC分类号： C12N9/88 , C07K2319/00 , C12N15/8243
摘要： The invention relates to high-level production of pHBA in green plants using a unique expression cassette. The latter comprises a chorismate pyruvate lyase (CPL) coding sequence operably linked to a suitable promoter capable of driving protein expression in higher plants. Additionally, the CPL cassette comprises a sequence encoding a chloroplast transit peptide, its natural cleavage site, and a small portion of the transit peptide donor protein fused to the N-terminus of CPL. The chloroplast targeting sequence targets the foreign protein to the chloroplast compartment and aids in its uptake into the organelle. The cleavage site is unique to the transit peptide, and cleavage of the chimeric protein encoded by the cassette at this site releases a novel polypeptide that has full enzyme activity, comprising the mature CPL enzyme and a small portion of the transit peptide donor.
摘要翻译： 本发明涉及使用独特的表达盒在绿色植物中高水平生产pHBA。 后者包括可操作地连接到能够驱动高等植物中的蛋白质表达的合适的启动子的草酸丙酸裂解酶（CPL）编码序列。 此外，CPL盒包含编码叶绿体转运肽，其天然切割位点的序列和与CPL的N末端融合的转运肽供体蛋白的一小部分。 叶绿体靶向序列将外来蛋白质靶向叶绿体隔室，并有助于其吸收到细胞器中。 切割位点对于转运肽是独特的，并且在该位点处由盒编码的嵌合蛋白质的切割释放出具有完整酶活性的新型多肽，其包含成熟的CPL酶和少量的转运肽供体。
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3.发明授权Method for increasing the ratio of p-hydroxybenzoic acid ester glucoside to total p-hydroxybenzoic acid glucose conjugates in p-hydroxybenzoic acid-producing microorganisms and green plant cells 有权增加对羟基苯甲酸酯葡糖苷与对羟基苯甲酸葡萄糖结合物在对羟基苯甲酸生产微生物和绿色植物细胞中的比例的方法公开(公告)号：US07435556B2
公开(公告)日：2008-10-14
申请号：US11403388
申请日：2006-04-13
申请人： Paul V. Viitanen , Drew Van Dyk , Knut Meyer
发明人： Paul V. Viitanen , Drew Van Dyk , Knut Meyer
IPC分类号： C12Q1/48 , C12P19/44 , C12N9/10 , C12N15/00 , C12N5/10 , C12N1/21 , C12N5/04 , C12P21/00 , C07K14/00 , C07H21/00
CPC分类号： C12N9/1051 , C07K2319/00 , C12N15/8243
摘要： This invention pertains to a method for increasing the ratio of the pHBA ester glucoside to total pHBA glucose conjugates in pHBA-producing microorganisms and green plant cells using nucleic acid fragments encoding plant glucosyltransferases that exhibit catalytic activity with p-hydroxybenzoic acid (pHBA) as a substrate and only attach glucose to the aromatic carboxyl group of pHBA, to form the pHBA glucose ester.
摘要翻译： 本发明涉及使用编码植物葡萄糖基转移酶的核酸片段来增加pHBA酯葡萄糖苷与总pHBA葡萄糖结合物在pHBA生成微生物和绿色植物细胞中的比例的方法，所述核酸片段与对羟基苯甲酸（pHBA）呈现催化活性， 底物并且仅将葡萄糖附着在pHBA的芳族羧基上，形成pHBA葡萄糖酯。
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公开(公告)号：US07135326B2
公开(公告)日：2006-11-14
申请号：US10359369
申请日：2003-02-06
申请人： Paul V. Viitanen , Knut Meyer , Drew E. Van Dyk
发明人： Paul V. Viitanen , Knut Meyer , Drew E. Van Dyk
CPC分类号： C12N9/1051 , C07K2319/00 , C12N15/8243
摘要： This invention pertains to nucleic acid fragments encoding plant glucosyltransferases, heretofore undescribed, that exhibit catalytic activity with p-hydroxybenzoic acid (pHBA) as a substrate and only attach glucose to the aromatic carboxyl group of pHBA, to form the pHBA glucose ester. These enzymes have potential applications both in vitro and in vivo, and their primary amino acid sequences can be used to identify other proteins that have similar kinetic properties.
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公开(公告)号：US06683231B2
公开(公告)日：2004-01-27
申请号：US09855341
申请日：2001-05-15
申请人： Knut Meyer , Paul V. Viitanen , Drew E. Van Dyk
发明人： Knut Meyer , Paul V. Viitanen , Drew E. Van Dyk
IPC分类号： A01H100
CPC分类号： C12N9/88 , C07K2319/00 , C12N15/8243
摘要： The invention relates to high-level production of pHBA in green plants using a unique expression cassette. The latter comprises a chorismate pyruvate lyase (CPL) coding sequence operably linked to a suitable promoter capable of driving protein expression in higher plants. Additionally, the CPL cassette comprises a sequence encoding a chloroplast transit peptide, its natural cleavage site, and a small portion of the transit peptide donor protein fused to the N-terminus of CPL. The chloroplast targeting sequence targets the foreign protein to the chloroplast compartment and aids in its uptake into the organelle. The cleavage site is unique to the transit peptide, and cleavage of the chimeric protein encoded by the cassette at this site releases a novel polypeptide that has full enzyme activity, comprising the mature CPL enzyme and a small portion of the transit peptide donor.
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6.发明授权Process for inducing cytochrome P-450 enzymes in streptomyces bacteria and determining the mutagenicity of chemicals 失效在链霉菌中诱导细胞色素P-450酶的方法，并测定化学物质的致突变性
公开(公告)号：US5169755A
公开(公告)日：1992-12-08
申请号：US207630
申请日：1988-06-16
IPC分类号： C12N15/09 , C12N1/21 , C12N9/02 , C12N15/76 , C12Q1/02 , C12Q1/10 , C12Q1/26 , C12R1/465 , C12R1/545
CPC分类号： C12N9/0077 , C12N15/76 , C12Q1/025 , C12Q1/10 , C12Q1/26 , G01N2333/255 , G01N2333/36 , G01N2333/795 , G01N2333/80 , G01N2333/902 , G01N2333/90245 , Y10S435/886 , Y10S435/897
摘要： A process for inducting cytochrome P-450 enzyme production in bacteria of the genus Streptomyces using inducers such as soybean flour, genistein or genistin is described. Uses for the cytochrome P-450 enzymes produced are also discussed as is a process for using genetically engineered Streptomyces to determine the mutagenicity of chemicals.
摘要翻译： 描述了使用诱导剂如大豆粉，染料木素或染料木素诱导链霉菌属细菌中细胞色素P-450酶产生的方法。 还讨论了生产的细胞色素P-450酶的用途，是使用遗传工程化链霉菌来确定化学品的致突变性的方法。
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公开(公告)号：US08247208B2
公开(公告)日：2012-08-21
申请号：US12641642
申请日：2009-12-18
申请人： Perry G. Caimi , Mark Emptage , Xu Li , Paul V. Viitanen , Yat-Chen Chou , Mary Ann Franden , Min Zhang
发明人： Perry G. Caimi , Mark Emptage , Xu Li , Paul V. Viitanen , Yat-Chen Chou , Mary Ann Franden , Min Zhang
摘要： Strains of xylose utilizing Zymomonas with improved xylose utilization and ethanol production during fermentation in stress conditions were obtained using an adaptation method. The adaptation involved continuously growing xylose utilizing Zymomonas in media containing high sugars, acetic acid, ammonia, and ethanol.
摘要翻译： 使用适应方法获得使用具有改善的木糖利用的发酵单菌的木糖菌株和在胁迫条件下发酵期间的乙醇生产。 适应性涉及使用含有高糖，乙酸，氨和乙醇的培养基中的酵母单胞菌连续生长木糖。
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公开(公告)号：US20110318801A1
公开(公告)日：2011-12-29
申请号：US13161749
申请日：2011-06-16
申请人： ROBEL Y. KAHSAY , Min Qi , Luan Tao , Paul V. Viitanen , Jianjun Yang
发明人： ROBEL Y. KAHSAY , Min Qi , Luan Tao , Paul V. Viitanen , Jianjun Yang
摘要： Zymomonas expressing xylose isomerase from A. missouriensis was found to have improved xylose utilization, growth, and ethanol production when grown in media containing xylose. Xylose isomerases related to that of A. missouriensis were identified structurally through molecular phylogenetic and Profile Hidden Markov Model analyses, providing xylose isomerases that may be used to improve xylose utilization.
摘要翻译： 发现来自A. missouriensis的表达木糖异构酶的发酵单胞菌在含有木糖的培养基中生长时具有改善的木糖利用率，生长和乙醇生产。 通过分子系统发育和轮廓隐马尔可夫模型分析，结构地鉴定了与密螺旋体相关的木糖异构酶，提供可用于改善木糖利用的木糖异构酶。
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公开(公告)号：US08623623B2
公开(公告)日：2014-01-07
申请号：US13161749
申请日：2011-06-16
申请人： Robel Y. Kahsay , Min Qi , Luan Tao , Paul V. Viitanen , Jianjun Yang
发明人： Robel Y. Kahsay , Min Qi , Luan Tao , Paul V. Viitanen , Jianjun Yang
IPC分类号： C12N9/00
摘要： Zymomonas expressing xylose isomerase from A. missouriensis was found to have improved xylose utilization, growth, and ethanol production when grown in media containing xylose. Xylose isomerases related to that of A. missouriensis were identified structurally through molecular phylogenetic and Profile Hidden Markov Model analyses, providing xylose isomerases that may be used to improve xylose utilization.
摘要翻译： 发现来自A. missouriensis的表达木糖异构酶的发酵单胞菌在含有木糖的培养基中生长时具有改善的木糖利用率，生长和乙醇生产。 通过分子系统发育和轮廓隐马尔可夫模型分析，结构地鉴定了与密螺旋体相关的木糖异构酶，提供可用于改善木糖利用的木糖异构酶。
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公开(公告)号：US07998722B2
公开(公告)日：2011-08-16
申请号：US12410501
申请日：2009-03-25
申请人： Paul V. Viitanen , Luan Tao , Yuying Zhang , Perry G. Caimi , Carol M. McCutchen , Laura McCole , Min Zhang , Yat-Chen Chou , Mary Ann Franden
发明人： Paul V. Viitanen , Luan Tao , Yuying Zhang , Perry G. Caimi , Carol M. McCutchen , Laura McCole , Min Zhang , Yat-Chen Chou , Mary Ann Franden
CPC分类号： C12N9/92 , C12N9/0008 , C12N9/1022 , C12N9/1205 , C12P7/065 , Y02E50/17
摘要： Strains of Zymomonas were engineered by introducing a chimeric xylose isomerase gene that contains a mutant promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene. The promoter directs increased expression of xylose isomerase, and when the strain is in addition engineered for expression of xylulokinase, transaldolase and transketolase, improved utilization of xylose is obtained.
摘要翻译： 通过引入含有运动发酵单胞菌甘油醛-3-磷酸脱氢酶基因的突变启动子的嵌合木糖异构酶基因工程化发酵单胞菌菌株。 启动子指导木糖异构酶的增加表达，并且当该菌株另外被设计用于表达木酮糖激酶，转醛醇酶和转酮酶时，获得木糖的改善的利用。
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