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公开(公告)号：US20100291536A1
公开(公告)日：2010-11-18
申请号：US12780345
申请日：2010-05-14
CPC分类号： A61B10/0096 , A61B10/0051 , A61B90/98 , B01L3/505 , B01L2200/0689 , B01L2300/021 , B01L2300/0864 , G01N2001/005
摘要： The present invention provides a method and device for collecting treating and analysis of biological or chemical material by introducing a source material into a specimen container, transferring the source material to a processing device and thermally, chemically and/or mechanically treating the source material to alter at least one constitutive characteristic of the source material and to release or create a target material from the source material.
摘要翻译： 本发明提供了一种通过将源材料引入到样本容器中来收集生物或化学材料的处理和分析的方法和装置，将源材料转移到处理装置，并且热，化学和/或机械地处理源材料以改变 源材料的至少一个构成特征并且从源材料释放或产生靶材料。
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12.发明授权Device and methods for subdividing and filtering gel material and extracting molecules therefrom 失效用于细分和过滤凝胶材料并从中提取分子的装置和方法公开(公告)号：US06758974B2
公开(公告)日：2004-07-06
申请号：US09906792
申请日：2001-07-18
IPC分类号： B01D1104
CPC分类号： C07K1/34 , Y10T436/25375 , Y10T436/255 , Y10T436/2575
摘要： An apparatus and method for mincing a gel includes a gel mincing tube and a mesh material. The mesh material extends across the end of the tube. To subdivide a gel using the mincing apparatus, a gel is placed upon the mesh material in the mincing tube, the mincing tube, mesh material and the gel are spun in a centrifuge, forcing the gel through the mesh material so that the gel is subdivided into generally uniform smaller fragments. The mesh material may be secured to a tube in the form of a nesting tube. The nesting tube nests within the opening of a recovery vessel. The mesh material may be placed in series with a conditionally porous membrane in the nesting tube. Centrifuging the nesting tube and the recovery vessel subdivides gel material into fragments by forcing the gel through the mesh material. The gel subsequently falls upon the membrane, and may be treated on the membrane to extract or otherwise treat analytes in the gel material.
摘要翻译： 用于切割凝胶的设备和方法包括凝胶切割管和网状材料。 网状材料延伸穿过管的端部。 为了使用切碎装置对凝胶进行细分，将凝胶放置在打孔管中的网状材料上，在离心机中将切细管，网状材料和凝胶纺丝，迫使凝胶通过网状材料，使凝胶细分 变成大致均匀的较小碎片。 网状材料可以以嵌套管的形式固定到管。 嵌套管嵌套在回收容器的开口内。 网状材料可以与嵌套管中的条状多孔膜串联放置。 将嵌套管和回收容器离心通过使凝胶通过网状材料将凝胶材料细分成碎片。 凝胶随后落在膜上，并且可以在膜上处理以提取或以其它方式处理凝胶材料中的分析物。
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公开(公告)号：US09034635B2
公开(公告)日：2015-05-19
申请号：US12918594
申请日：2009-02-19
CPC分类号： B01L7/52 , B01L3/505 , B01L2300/043 , B01L2300/0627 , B01L2300/0838 , B01L2300/18 , B01L2300/1822 , B01L2300/1844
摘要： A thermocycler apparatus and method for rapidly performing the PCR process employs at least two thermoelectric modules which are in substantial spatial opposition with an interior space present between opposing modules. One or multiple sample vessels are placed in between the modules such that the vessels are subjected to temperature cycling by the modules. The sample vessels have a minimal internal dimension that is substantially perpendicular to the modules that facilitates rapid temperature cycling. In embodiments of the invention the sample vessels may be deformable between: a) a shape having a wide mouth to facilitate filling and removing of sample fluids from the vessel, and b) a shape which is thinner for conforming to the sample cavity or interior space between the thermoelectric modules of the thermocycler for more rapid heat transfer.
摘要翻译： 用于快速执行PCR过程的热循环仪装置和方法采用至少两个热电模块，其与存在于相对模块之间的内部空间实质上空间相对。 将一个或多个样品容器放置在模块之间，使得容器经过模块的温度循环。 样品容器具有基本垂直于促进快速温度循环的模块的最小内部尺寸。 在本发明的实施例中，样品容器可以在以下之间变形：a）具有宽口的形状，以便于从容器中填充和除去样品流体，以及b）更薄的形状，以符合样品腔或内部空间 在热循环器的热电模块之间用于更快速的热传递。
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公开(公告)号：US08889086B2
公开(公告)日：2014-11-18
申请号：US13452419
申请日：2012-04-20
CPC分类号： B01L3/50825 , B01L7/52 , B01L2300/022 , B01L2300/043 , B01L2300/123 , B01L2300/168
摘要： An improved tube including a closure portion, a strap integrally connected to the closure portion and being configured for defining a living hinge, a body portion having a longitudinal axis and an outer wall generally circumscribing the longitudinal axis, and being integrally and hingedly connected with the closure portion by way of the strap. The body portion including a sample portion being generally elongated along the longitudinal axis and being configured for elastic deformation along a portion of its length, including in a direction that is generally transverse to the longitudinal axis so that at least a portion of the wall structure compressively and resiliently deforms and engages a wall defining an opening in a sample block of a polymerase chain reaction amplification device, and the first outer wall dimension of the sample portion reduces to a smaller second outer wall dimension.
摘要翻译： 一种改进的管，其包括封闭部分，整体地连接到所述封闭部分并且构造成用于限定活动铰链的带，具有纵向轴线的主体部分和通常围绕所述纵向轴线的外壁，并且与所述主体部分整体地和铰接地连接 通过带子的封闭部分。 主体部分包括沿着纵向轴线大致细长的样本部分，并且构造成沿其长度的一部分弹性变形，包括沿大致横向于纵向轴线的方向弹性变形，使得壁结构的至少一部分被压缩 并且弹性变形并且接合限定聚合酶链式反应扩增装置的样品块中的开口的壁，并且样品部分的第一外壁尺寸减小到更小的第二外壁尺寸。
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公开(公告)号：US09737891B2
公开(公告)日：2017-08-22
申请号：US13484963
申请日：2012-05-31
CPC分类号： B01L7/52 , B01L2200/14 , B01L2300/0627 , B01L2300/0858 , B01L2300/1805 , B01L2300/1822 , C12Q1/6844 , G01N2035/00366
摘要： A thermocycling device and method of operating a thermocycler instrument, the instrument including a sample holder, at least one thermal cycling element, and at least one first and second temperature sensors, for causing the sample holder containing the at least one sample to undergo polymerase chain reaction amplification by repeated cycling between at least a denaturation heating stage and an annealing cooling stage. The first temperature corresponding with the temperature of the sample holder is monitored using the at least one first temperature sensor, and a second temperature corresponding with the temperature external of the sample holder is monitored using the at least one second temperature sensor. Based upon the first temperature and the second temperature, the power that is delivered to the at least one thermal cycling element of the instrument is dynamically controlled.
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16.发明申请Rapid Thermocycler System for Rapid Amplification of Nucleic Acids and Related Methods 有权用于快速扩增核酸的快速热循环仪系统及相关方法公开(公告)号：US20120308990A1
公开(公告)日：2012-12-06
申请号：US13484963
申请日：2012-05-31
IPC分类号： C12Q3/00
CPC分类号： B01L7/52 , B01L2200/14 , B01L2300/0627 , B01L2300/0858 , B01L2300/1805 , B01L2300/1822 , C12Q1/6844 , G01N2035/00366
摘要： A thermocycling device and method of operating a thermocycler instrument, the instrument including a sample holder, at least one thermal cycling element, and at least one first and second temperature sensors, for causing the sample holder containing the at least one sample to undergo polymerase chain reaction amplification by repeated cycling between at least a denaturation heating stage and an annealing cooling stage. The first temperature corresponding with the temperature of the sample holder is monitored using the at least one first temperature sensor, and a second temperature corresponding with the temperature external of the sample holder is monitored using the at least one second temperature sensor. Based upon the first temperature and the second temperature, the power that is delivered to the at least one thermal cycling element of the instrument is dynamically controlled.
摘要翻译： 一种热循环装置和操作热循环仪器的方法，所述仪器包括样品保持器，至少一个热循环元件和至少一个第一和第二温度传感器，用于使含有至少一个样品的样品架进行聚合酶链 在至少变性加热阶段和退火冷却阶段之间重复循环的反应扩增。 使用至少一个第一温度传感器监测与样品保持器的温度相对应的第一温度，并且使用至少一个第二温度传感器监测与样品保持器外部的温度相对应的第二温度。 基于第一温度和第二温度，动态地控制输送到仪器的至少一个热循环元件的功率。
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公开(公告)号：US20110039305A1
公开(公告)日：2011-02-17
申请号：US12918594
申请日：2009-02-19
CPC分类号： B01L7/52 , B01L3/505 , B01L2300/043 , B01L2300/0627 , B01L2300/0838 , B01L2300/18 , B01L2300/1822 , B01L2300/1844
摘要： A thermocycler apparatus and method for rapidly performing the PCR process employs at least two thermoelectric modules which are in substantial spatial opposition with an interior space present between opposing modules. One or multiple sample vessels are placed in between the modules such that the vessels are subjected to temperature cycling by the modules. The sample vessels have a minimal internal dimension that is substantially perpendicular to the modules that facilitates rapid temperature cycling. In embodiments of the invention the sample vessels may be deformable between: a) a shape having a wide mouth to facilitate filling and removing of sample fluids from the vessel, and b) a shape which is thinner for conforming to the sample cavity or interior space between the thermoelectric modules of the thermocycler for more rapid heat transfer.
摘要翻译： 用于快速执行PCR过程的热循环仪装置和方法采用至少两个热电模块，其与存在于相对模块之间的内部空间实质上空间相对。 将一个或多个样品容器放置在模块之间，使得容器经过模块的温度循环。 样品容器具有基本垂直于促进快速温度循环的模块的最小内部尺寸。 在本发明的实施例中，样品容器可以在以下之间变形：a）具有宽口的形状，以便于从容器中填充和除去样品流体，以及b）更薄的形状，以符合样品腔或内部空间 在热循环器的热电模块之间用于更快速的热传递。
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公开(公告)号：US20120269703A1
公开(公告)日：2012-10-25
申请号：US13452419
申请日：2012-04-20
IPC分类号： B01L3/14
CPC分类号： B01L3/50825 , B01L7/52 , B01L2300/022 , B01L2300/043 , B01L2300/123 , B01L2300/168
摘要： An improved tube including a closure portion, a strap integrally connected to the closure portion and being configured for defining a living hinge, a body portion having a longitudinal axis and an outer wall generally circumscribing the longitudinal axis, and being integrally and hingedly connected with the closure portion by way of the strap. The body portion including a sample portion being generally elongated along the longitudinal axis and being configured for elastic deformation along a portion of its length, including in a direction that is generally transverse to the longitudinal axis so that at least a portion of the wall structure compressively and resiliently deforms and engages a wall defining an opening in a sample block of a polymerase chain reaction amplification device, and the first outer wall dimension of the sample portion reduces to a smaller second outer wall dimension.
摘要翻译： 一种改进的管，其包括封闭部分，整体地连接到所述封闭部分并且构造成用于限定活动铰链的带，具有纵向轴线的主体部分和通常围绕所述纵向轴线的外壁，并且与所述主体部分整体地和铰接地连接 通过带子的封闭部分。 主体部分包括沿着纵向轴线大致细长的样本部分，并且构造成沿其长度的一部分弹性变形，包括沿大致横向于纵向轴线的方向弹性变形，使得壁结构的至少一部分被压缩 并且弹性变形并且接合限定聚合酶链式反应扩增装置的样品块中的开口的壁，并且样品部分的第一外壁尺寸减小到更小的第二外壁尺寸。
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19.发明授权Device and methods for subdividing and filtering gel material and extracting molecules therefrom 失效用于细分和过滤凝胶材料并从中提取分子的装置和方法公开(公告)号：US06916423B2
公开(公告)日：2005-07-12
申请号：US09906792
申请日：2001-07-18
IPC分类号： G01N33/48 , C07K1/34 , C12M1/00 , C12M1/33 , C12N15/09 , C12Q1/68 , G01N1/10 , G01N1/28 , B01D11/04 , G01N27/26
CPC分类号： C07K1/34 , Y10T436/25375 , Y10T436/255 , Y10T436/2575
摘要： An apparatus and method for mincing a gel includes a gel mincing tube and a mesh material. The mesh material extends across the end of the tube. To subdivide a gel using the mincing apparatus, a gel is placed upon the mesh material in the mincing tube, the mincing tube, mesh material and the gel are spun in a centrifuge, forcing the gel through the mesh material so that the gel is subdivided into generally uniform smaller fragments. The mesh material may be secured to a tube in the form of a nesting tube. The nesting tube nests within the opening of a recovery vessel. The mesh material may be placed in series with a conditionally porous membrane in the nesting tube. Centrifuging the nesting tube and the recovery vessel subdivides gel material into fragments by forcing the gel through the mesh material. The gel subsequently falls upon the membrane, and may be treated on the membrane to extract or otherwise treat analytes in the gel material.
摘要翻译： 用于切割凝胶的设备和方法包括凝胶切割管和网状材料。 网状材料延伸穿过管的端部。 为了使用切碎装置对凝胶进行细分，将凝胶放置在打孔管中的网状材料上，在离心机中将切细管，网状材料和凝胶纺丝，迫使凝胶通过网状材料，使凝胶细分 变成大致均匀的较小碎片。 网状材料可以以嵌套管的形式固定到管。 嵌套管嵌套在回收容器的开口内。 网状材料可以与嵌套管中的条状多孔膜串联放置。 将嵌套管和回收容器离心通过使凝胶通过网状材料将凝胶材料细分成碎片。 凝胶随后落在膜上，并且可以在膜上处理以提取或以其它方式处理凝胶材料中的分析物。
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