-
11.发明申请Compositions, Methods and Kits for Real-Time Nucleic Acid Analysis in Live Cells 审中-公开用于实时细胞中实时核酸分析的组合物，方法和试剂盒公开(公告)号：US20160168558A1
公开(公告)日：2016-06-16
申请号：US14985826
申请日：2015-12-31
发明人： James Eberwine , Ulo Langel , Emelia Eiriksdottir , Tiina Peritz [nee Pajunen] , Jai-Yoon Sul , Philip G. Haydon , Junhyong Kim
CPC分类号： C12N9/96 , C07K2319/72 , C07K2319/73 , C07K2319/85 , C12N9/1247 , C12N9/127 , C12Q1/68 , C12Y207/07006
摘要： The present invention includes compositions, methods and kits for the real-time detection of transcription and translation in live cells, tissues and organisms. The present invention further provides method for the rapid sequencing of nucleic acids without using conventional sequencing techniques or reactions.
摘要翻译： 本发明包括用于在活细胞，组织和生物体中实时检测转录和翻译的组合物，方法和试剂盒。 本发明还提供了不使用常规测序技术或反应快速测序核酸的方法。
-
公开(公告)号：US20140206059A1
公开(公告)日：2014-07-24
申请号：US14230848
申请日：2014-03-31
发明人： James Eberwine , Jai-Yoon Sul , Chia-Wen Wu , Fanyi Zeng , Junhyong Kim
IPC分类号： C12N5/0793 , C12N5/079 , C12N5/077
CPC分类号： C12N5/062 , C12N5/0622 , C12N5/0657 , C12N2501/998 , C12N2506/00 , C12N2506/08 , C12N2506/1307
摘要： The present invention includes methods for effecting phenotype conversion in a cell by transfecting the cell with phenotype-converting nucleic acid. Expression of the nucleic acids results in a phenotype conversion in the transfected cell. Preferably the phenotype-converting nucleic acid is a transcriptome, and more preferably an mRNA transcriptome.
摘要翻译： 本发明包括通过用表型转化核酸转染细胞来实现细胞中表型转化的方法。 核酸的表达导致转染细胞中的表型转化。 表型转换核酸优选是转录组，更优选mRNA转录组。
-
公开(公告)号：US20240166678A1
公开(公告)日：2024-05-23
申请号：US18522844
申请日：2023-11-29
发明人： James Eberwine , Ivan J. Dmochowski , Sean B. Yeldell , Julianne C. Griepenburg , Teresa L. Rapp , Jennifer M. Singh , Jai-Yoon Sul , Jaehee Lee
IPC分类号： C07F15/00 , B01J35/39 , C07H23/00 , C12N13/00 , C12N15/113 , C12Q1/6818 , C12Q1/6823 , C12Q1/6825
CPC分类号： C07F15/0053 , B01J35/39 , C07H23/00 , C12N13/00 , C12N15/113 , C12Q1/6818 , C12Q1/6823 , C12Q1/6825 , B01J2531/821 , C12N2310/11 , C12N2310/32 , C12N2310/3233 , C12N2310/351 , C12N2310/3517 , C12N2310/531
摘要： Compositions and methods of capturing one or more nucleic acid molecules of a cell or subcellular compartment are described. In certain aspects, the compositions comprise a caged molecule comprising one or more photolinkers and an antisense oligonucleotide, which when uncaged hybridizes to a target nucleic acid molecule.
-
公开(公告)号：US11739321B2
公开(公告)日：2023-08-29
申请号：US16736085
申请日：2020-01-07
发明人： James Eberwine , Jae-Hee Lee , Jifen Li , Stephen Fisher , Youtao Lu , Junhyong Kim , Jai-Yoon Sul , Jinchun Wang , Mimi Healy
IPC分类号： C12Q1/6816 , C12N15/11 , C07H19/10 , C07H21/02 , C07H21/04
CPC分类号： C12N15/11 , C07H19/10 , C12Q1/6816 , C07H21/02 , C07H21/04 , C12Y207/07006 , C12Y207/07007 , C12Y207/07049
摘要： Provided herein are compositions and methods to assess the genomic landscape of fixed cells using light activated oligonucleotides that can be directed to the nucleus, mitochondria, or cytoplasm of fixed cells and that, upon activation, can be extended for in situ copying of nuclear single-stranded DNA (i.e., open chromatin), open mitochondrial DNA, and/or cytoplasmic RNA into barcoded complementary DNA. These methods also provide for gene specific 3D chromatin structural niche analysis.
-
公开(公告)号：US20200206364A1
公开(公告)日：2020-07-02
申请号：US16814120
申请日：2020-03-10
发明人： James Eberwine , Philip G. Haydon , Jai-Yoon Sul , Hajime Takano , Chia-Wen Kitty Wu , Fanyi Zeng
摘要： The present invention includes methods for transferring a multigenic phenotype to a cell by transfecting, preferably by phototransfection, and locally transfecting a cell or a cellular process with a laser while the cell is bathed in a fluid medium comprising two or more nucleic acids, thereby introducing the nucleic acid into the interior of the cell. Expression of the nucleic acids results in a multigenic phenotype in the transfected cell.
-
公开(公告)号：US09845480B2
公开(公告)日：2017-12-19
申请号：US14880737
申请日：2015-10-12
发明人： James Eberwine , Jai-Yoon Sul , Tae Kyung Kim , Vickas Patel
CPC分类号： C12N15/85 , A61K31/713 , A61K45/06 , A61K48/00 , C12N5/0622 , C12N5/0657 , C12N2501/998 , C12N2506/00 , C12N2506/08 , C12N2506/1307
摘要： The present invention includes methods for effecting phenotype conversion in a cell by transfecting the cell with phenotype-converting nucleic acid. Expression of the nucleic acids results in a phenotype conversion in the transfected cell. Preferably the phenotype-converting nucleic acid is a transcriptome, and more preferably an mRNA transcriptome.
-
-
-
-
-
搜索结果
16 条记录 (耗时 0.034 秒)
