公开(公告)号：US11591372B2

公开(公告)日：2023-02-28

申请号：US16877319

申请日：2020-05-18

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： Peter Collins ,  Bo Liang ,  Shirin Munir ,  Anne Schaap Nutt ,  Ursula Buchholz ,  Natalie Mackow ,  Peter Kwong ,  Barney Graham ,  Jason McLellan

IPC分类号： C07K14/005 ,  A61K39/12 ,  A61P31/14 ,  A61K39/155 ,  A61K39/00

摘要： Recombinant paramyxoviruses including a viral genome encoding a heterologous gene are provided. In several embodiments, the recombinant paramyxovirus is a recombinant parainfluenza virus, such as a recombinant PIV3 including a viral genome encoding a heterologous respiratory syncytial virus F ectodomain linked to the transmembrane domain and the cytoplasmic tail of the F protein from the PIV3. Nucleic acid molecules including the genome of a recombinant paramyxoviruses are also provided. The recombinant viruses may advantageously be used in vaccine formulations, such as for vaccines against parainfluenza virus and respiratory syncytial virus.

公开(公告)号：US10654898B2

公开(公告)日：2020-05-19

申请号：US15545218

申请日：2016-01-20

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： Peter Collins ,  Bo Liang ,  Shirin Munir ,  Anne Schaap Nutt ,  Ursula Buchholz ,  Natalie Mackow ,  Peter Kwong ,  Barney Graham ,  Jason McLellan

IPC分类号： C07K14/005 ,  A61P31/14 ,  A61K39/155 ,  A61K39/12 ,  A61K39/00

摘要： Recombinant paramyxoviruses including a viral genome encoding a heterologous gene are provided. In several embodiments, the recombinant paramyxovirus is a recombinant parainfluenza virus, such as a recombinant PIV3 including a viral genome encoding a heterologous respiratory syncytial virus F ectodomain linked to the transmembrane domain and the cytoplasmic tail of the F protein from the PIV3. Nucleic acid molecules including the genome of a recombinant paramyxoviruses are also provided. The recombinant viruses may advantageously be used in vaccine formulations, such as for vaccines against parainfluenza virus and respiratory syncytial virus.

公开(公告)号：US09738689B2

公开(公告)日：2017-08-22

申请号：US14207372

申请日：2014-03-12

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： Peter Kwong ,  Barney Graham ,  Jason McLellan ,  Man Chen ,  Michael Gordon Joyce ,  Baoshan Zhang ,  Tongqing Zhou

IPC分类号： C07K14/005

CPC分类号： C07K14/005 ,  C07K2319/03 ,  C07K2319/21 ,  C07K2319/40 ,  C07K2319/50 ,  C07K2319/70 ,  C12N2760/18522 ,  C12N2760/18534

摘要： Disclosed are immunogens including a recombinant RSV F protein stabilized in a prefusion conformation. Also disclosed are nucleic acids encoding the immunogens and methods of producing the immunogens. Methods for generating an immune response in a subject are also disclosed. In some embodiments, the method is a method for treating or preventing a RSV infection in a subject by administering a therapeutically effective amount of the immunogen to the subject.

公开(公告)号：US12077572B2

公开(公告)日：2024-09-03

申请号：US17952308

申请日：2022-09-25

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services ,  Korea (Center for Disease Control and Prevention)

发明人： Hansaem Lee ,  Janghoon Choi ,  Sungsoon Kim ,  Lingshu Wang ,  Barney Graham ,  John R Mascola

IPC分类号： C07K16/10 ,  G01N33/569

CPC分类号： C07K16/10 ,  G01N33/56983 ,  C07K2317/565 ,  C07K2317/76 ,  G01N2333/165

摘要： The present invention relates to monoclonal antibodies for a spike protein of the Middle East respiratory syndrome coronavirus (MERS-CoV), and a use thereof. Particularly, monoclonal antibodies 77-A5, 77-A6, 90-A3, 90-A9, 90-B2, 90-B7, 90-C4, 90-E5, 90-E6, 90-F1 and 90-F2 according to the present invention have excellent attachment force with respect to a full-length spike protein of MERS-CoV and the S1 domain of the protein, and, of the monoclonal antibodies, the monoclonal antibodies 90-F1, 90-E5, 90-E6, 90-F2, 77-A5 and 77-A6 have excellent attachment force with respect to an RBD antigen of MERS-CoV. Also, the antibodies 77-A5, 77-A6, 90-E5, 90-E6, 90-F1 and 90-F2 exhibit neutralizing capacity with respect to a MERS pseudovirus and MERS-CoV, and the antibodies 90-B2 and 90-B7 exhibit neutralizing capacity only with respect to MERS-CoV. Further, the monoclonal antibodies have a particular monomeric form, and have excellent stability and thus may be useful for treating or diagnosing MERS.

公开(公告)号：US10961283B2

公开(公告)日：2021-03-30

申请号：US16312166

申请日：2017-06-27

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： Peter Kwong ,  Ivelin Georgiev ,  Michael Gordon Joyce ,  Masaru Kanekiyo ,  Aliaksandr Druz ,  Ulrich Baxa ,  Joseph Van Galen ,  Cheng Cheng ,  John Mascola ,  Yaroslav Tsybovsky ,  Yongping Yang ,  Barney Graham ,  Syed Mohammad Moin ,  Jeffrey Boyington

IPC分类号： C07K14/11 ,  A61K39/21 ,  C12N7/00 ,  C07K14/08 ,  C07K14/16 ,  C07K14/165 ,  C07K14/435 ,  A61K39/00

摘要： Disclosed are recombinant insect ferritin nanoparticles that can be used to display two different trimeric antigens at an equal ratio. Also disclosed are nucleic acids encoding the recombinant insect ferritin nanoparticles and methods of producing the recombinant insect ferritin nanoparticles. Methods for eliciting an immune response in a subject are also provided.

公开(公告)号：US10759846B2

公开(公告)日：2020-09-01

申请号：US16403211

申请日：2019-05-03

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： Barney Graham ,  Wing-Pui Kong ,  Kayvon Modjarrad ,  Lingshu Wang ,  Wei Shi ,  Michael Gordon Joyce ,  Masaru Kanekiyo ,  John Mascola

IPC分类号： C07K16/10 ,  A61K39/12 ,  C07K14/165 ,  A61K39/215 ,  C07K14/005 ,  G01N33/569 ,  A61K39/00

摘要： Methods of inducing an immune response in a subject to the Middle East respiratory syndrome coronavirus (MERS-CoV) are provided. In several embodiments, the immune response is a protective immune response that inhibits or prevents MERS-CoV infection in the subject. Recombinant MERS-CoV polypeptides and nucleic acid molecules encoding same are also provided. Additionally, neutralizing antibodies that specifically bind to MERS-CoV S protein and antigen binding fragments thereof are disclosed. The antibodies and antigen binding fragments are useful, for example, in methods of detecting MERS-CoV S protein in a sample or in a subject, as well as methods of preventing and treating a MERS-CoV infection in a subject.

公开(公告)号：US10301377B2

公开(公告)日：2019-05-28

申请号：US15553466

申请日：2016-02-24

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： Barney Graham ,  Wing-Pui Kong ,  Kayvon Modjarrad ,  Lingshu Wang ,  Wei Shi ,  Michael Gordon Joyce ,  Masaru Kanekiyo ,  John Mascola

IPC分类号： A61K39/12 ,  C07K16/10 ,  C07K14/165 ,  A61K39/215 ,  C07K14/005 ,  G01N33/569 ,  A61K39/00

摘要： Methods of inducing an immune response in a subject to the Middle East respiratory syndrome coronavirus (MERS-CoV) are provided. In several embodiments, the immune response is a protective immune response that inhibits or prevents MERS-CoV infection in the subject. Recombinant MERS-CoV polypeptides and nucleic acid molecules encoding same are also provided. Additionally, neutralizing antibodies that specifically bind to MERS-CoV S protein and antigen binding fragments thereof are disclosed. The antibodies and antigen binding fragments are useful, for example, in methods of detecting MERS-CoV S protein in a sample or in a subject, as well as methods of preventing and treating a MERS-CoV infection in a subject.

公开(公告)号：US11878998B2

公开(公告)日：2024-01-23

申请号：US17524380

申请日：2021-11-11

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： Peter Kwong ,  Barney Graham ,  John Mascola ,  Li Ou ,  Aliaksandr Druz ,  Man Chen ,  Wing-Pui Kong ,  Ivelin Stefanov Georgiev ,  Emily Rundlet ,  Michael Gordon Joyce ,  Yaroslav Tsybovsky ,  Paul Thomas ,  Marie Pancera ,  Mallika Sastry ,  Cinque Soto ,  Joseph Van Galen ,  Guillaume Stewart-Jones ,  Yongping Yang ,  Baoshan Zhang ,  Ulrich Baxa

IPC分类号： C07K14/005 ,  A61K39/12 ,  A61P37/04 ,  A61P31/14 ,  A61K39/00

CPC分类号： C07K14/005 ,  A61K39/12 ,  A61P31/14 ,  A61P37/04 ,  A61K39/00 ,  A61K2039/5252 ,  A61K2039/5254 ,  A61K2039/53 ,  A61K2039/543 ,  A61K2039/55505 ,  A61K2039/55561 ,  A61K2039/55566 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/50 ,  C07K2319/70 ,  C07K2319/735 ,  C12N2710/10343 ,  C12N2760/18522 ,  C12N2760/18523 ,  C12N2760/18534

摘要： Embodiments of a recombinant Respiratory Syncytial Virus (RSV) F ectodomain trimer stabilized in a prefusion conformation are provided. Also disclosed are nucleic acids encoding the RSV F ectodomain trimer and methods of producing the RSV F ectodomain trimer. Methods for inducing an immune response in a subject are also disclosed. In some embodiments, the method can be a method for treating or preventing a RSV infection in a subject by administering a therapeutically effective amount of the recombinant RSV F ectodomain trimer to the subject.

公开(公告)号：US20210275664A1

公开(公告)日：2021-09-09

申请号：US17194834

申请日：2021-03-08

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Servic ,  The Scripps Research Institute ,  Trustees of Dartmouth College

发明人： Barney Graham ,  Jason McLellan ,  Andrew Ward ,  Robert Kirchdoerfer ,  Christopher Cottrell ,  Michael Gordon Joyce ,  Masaru Kanekiyo ,  Nianshuang Wang ,  Jesper Pallesen ,  Hadi Yassine ,  Hannah Turner ,  Kizzmekia Corbett

IPC分类号： A61K39/215 ,  A61P31/14 ,  C07K14/005 ,  C12N7/00

摘要： Coronavirus S ectodomain trimers stabilized in a prefusion conformation, nucleic acid molecules and vectors encoding these proteins, and methods of their use and production are disclosed. In several embodiments, the coronavirus S ectodomain trimers and/or nucleic acid molecules can be used to generate an immune response to coronavirus in a subject. In additional embodiments, the therapeutically effective amount of the coronavirus S ectodomain trimers and/or nucleic acid molecules can be administered to a subject in a method of treating or preventing coronavirus infection.

公开(公告)号：US20200061185A1

公开(公告)日：2020-02-27

申请号：US16344774

申请日：2017-10-25

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Servic ,  The Scripps Research Institute ,  Trustees of Dartmouth College

发明人： Barney Graham ,  Jason McLellan ,  Andrew Ward ,  Robert Kirchdoerfer ,  Christopher Cottrell ,  Michael Gordon Joyce ,  Masaru Kanekiyo ,  Nianshuang Wang ,  Jesper Pallesen ,  Hadi Yassine ,  Hannah Turner ,  Kizzmekia Corbett

IPC分类号： A61K39/215 ,  C07K14/005 ,  A61P31/14 ,  C12N7/00

摘要： Coronavirus S ectodomain trimers stabilized in a prefusion conformation, nucleic acid molecules and vectors encoding these proteins, and methods of their use and production are disclosed. In several embodiments, the coronavirus S ectodomain trimers and/or nucleic acid molecules can be used to generate an immune response to coronavirus in a subject. In additional embodiments, the therapeutically effective amount of the coronavirus S ectodomain trimers and/or nucleic acid molecules can be administered to a subject in a method of treating or preventing coronavirus infection.