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19 条记录 (耗时 0.022 秒)

    Methods for producing Humicola lipases in aspergillus
    13.
    发明授权
    Methods for producing Humicola lipases in aspergillus 失效
    在曲霉菌中生产腐质霉脂肪酶的方法

    公开(公告)号：US5965384A

    公开(公告)日：1999-10-12

    申请号：US463172

    申请日：1995-06-05

    申请人： Esper Boel ,  Tove Christensen ,  Helle Fabricius Woldike ,  Ida Birgitte Huge-Jensen

    发明人： Esper Boel ,  Tove Christensen ,  Helle Fabricius Woldike ,  Ida Birgitte Huge-Jensen

    IPC分类号： A01H5/02 ,  C12N1/15 ,  C12N9/20 ,  C12N9/28 ,  C12N9/30 ,  C12N9/64 ,  C12N15/52 ,  C12N15/67 ,  C12N15/80 ,  C12P21/02

    CPC分类号： C12N9/20 ,  A01H5/02 ,  C12N15/67 ,  C12N15/80 ,  C07K2319/02

    摘要： A process for expression of a protein product in Aspergillus oryzae is disclosed. The process comprises transforming Aspergillus oryzae with a vector system comprising DNA sequences encoding functions facilitating gene expression, a suitable marker for selection of transformants, and a DNA sequence encoding the desired protein product. The process enables industrial production of many different polypeptides and proteins in A. oryzae. Examples of such products are chymosin or prochymosin and other rennets, proteases, lipases and amylases. Also disclosed is an effective promoter for expression of a protein in Aspergillus. A preferred promoter is the TAKA-amylase promoter or functional parts thereof. There is also provided a process for the production of a recombinant Humicola lipase. The recombinant Humicola lipase from A. oryzae differs from the native lipase in having a greater glycosylation and in exhibiting an improved thermostability.

    摘要翻译： 公开了在米曲霉中表达蛋白质产物的方法。 该方法包括用包含编码促进基因表达功能的DNA序列的载体系统，用于选择转化体的合适标记物和编码所需蛋白质产物的DNA序列来转化米曲霉。 该方法能够在米曲霉中工业生产许多不同的多肽和蛋白质。 这些产品的实例是凝乳酶或凝乳酶原和其他雷诺丝，蛋白酶，脂肪酶和淀粉酶。 还公开了用于在曲霉中表达蛋白质的有效启动子。 优选的启动子是TAKA-淀粉酶启动子或其功能部分。 还提供了用于生产重组Humicola脂肪酶的方法。 来自米曲霉的重组Humicola脂肪酶与天然脂肪酶的不同之处在于具有更大的糖基化并具有改善的热稳定性。

    Isolated transcription factor for an alpha-amylase promoter in filamentous fungi
    17.
    发明授权
    Isolated transcription factor for an alpha-amylase promoter in filamentous fungi 有权
    丝状真菌中α-淀粉酶启动子的分离转录因子

    公开(公告)号：US06555657B2

    公开(公告)日：2003-04-29

    申请号：US09920581

    申请日：2001-08-01

    申请人： Tove Christensen

    发明人： Tove Christensen

    IPC分类号： C07K1437

    CPC分类号： C12N9/20 ,  C07K14/38 ,  C12N15/80

    摘要： The present invention relates to a transcription factor found in filamentous fungi, especially in Aspergillii, DNA sequences coding for said factor, its transformation into and expression in fungal host organisms, and the use of said factor in such hosts for increasing the expression of a polypeptide of interest being produced by said host.

    摘要翻译： 本发明涉及在丝状真菌，特别是曲霉属中发现的转录因子，编码所述因子的DNA序列，其在真菌宿主生物体中的转化和表达，以及所述因子在这些宿主中用于增加多肽表达的转录因子 感兴趣的是由所述主机生产的。

    Nucleic acid encoding a recombinant humicola sp. lipase
    18.
    发明授权
    Nucleic acid encoding a recombinant humicola sp. lipase 失效
    编码重组腐质霉的核酸 脂肪酶

    公开(公告)号：US5874558A

    公开(公告)日：1999-02-23

    申请号：US650086

    申请日：1996-05-17

    申请人： Esper Boel ,  Tove Christensen ,  Helle Fabricius Woldike

    发明人： Esper Boel ,  Tove Christensen ,  Helle Fabricius Woldike

    IPC分类号： A01H5/02 ,  C12N9/20 ,  C12N9/28 ,  C12N9/30 ,  C12N9/64 ,  C12N15/52 ,  C12N15/67 ,  C12N15/80 ,  C07H21/04

    CPC分类号： C12N9/2417 ,  A01H5/02 ,  C12N15/52 ,  C12N15/67 ,  C12N15/80 ,  C12N9/20 ,  C12N9/242 ,  C12N9/6481 ,  C12N9/6483 ,  C12Y304/23004 ,  C07K2319/02

    摘要： A process for expression of a protein product in Aspergillus oryzae is disclosed. The process comprises transforming Aspergillus oryzae with a vector system comprising DNA-sequences encoding functions facilitating gene expression, a suitable marker for selection of transformants, and a DNA-sequence encoding the desired protein product. The process enables industrial production of many different polypeptides and proteins in A. oryzae. Examples of such products are chymosin or prochymosin and other rennets, proteases, lipases and amylases. Also disclosed is an effective promoter for expression of a protein in Aspergillus. A preferred promoter is the TAKA-amylase promoter or functional parts thereof. There is also provided a process for the production of a recombinant Humicola lipase. The recombinant Humicola lipase from A. oryzae differs from the native lipase in having a greater glycosylation and in exhibiting an improved thermostability.

    摘要翻译： 公开了在米曲霉中表达蛋白质产物的方法。 该方法包括用包含编码促进基因表达的功能的DNA序列的载体系统，选择转化体的合适标记物和编码所需蛋白质产物的DNA序列来转化米曲霉。 该方法能够在米曲霉中工业生产许多不同的多肽和蛋白质。 这些产品的实例是凝乳酶或凝乳酶原和其他雷诺丝，蛋白酶，脂肪酶和淀粉酶。 还公开了用于在曲霉中表达蛋白质的有效启动子。 优选的启动子是TAKA-淀粉酶启动子或其功能部分。 还提供了用于生产重组Humicola脂肪酶的方法。 来自米曲霉的重组Humicola脂肪酶与天然脂肪酶的不同之处在于具有更大的糖基化并具有改善的热稳定性。