公开(公告)号：US09612239B2

公开(公告)日：2017-04-04

申请号：US14001566

申请日：2012-02-27

申请人： Joannes Baptist Adrianus Dionisius Van Zon ,  Toon Hendrik Evers ,  Ron Martinus Laurentius Van Lieshout ,  Wilhelmina Maria Hardeman ,  Derk Jan Wilfred Klunder

发明人： Joannes Baptist Adrianus Dionisius Van Zon ,  Toon Hendrik Evers ,  Ron Martinus Laurentius Van Lieshout ,  Wilhelmina Maria Hardeman ,  Derk Jan Wilfred Klunder

IPC分类号： G01N21/17 ,  G01N33/543 ,  G01N21/552 ,  G01N21/64 ,  G01N21/77 ,  G01N27/74

CPC分类号： G01N33/54326 ,  G01N21/552 ,  G01N21/648 ,  G01N21/7703 ,  G01N27/745

摘要： The invention relates to a substance determining apparatus (19) for determining a substance within a fluid. A location event determination unit (34) determines location events indicating that a particle, which may have attached the substance, is located in a sensing region and a property of the location events from the sensing signal generated by sensing the sensing region. A location events selection unit (70) selects a part of the location events having a property in a predefined property range, and a substance determination unit (40) determines the substance within the fluid (3) based on the selected part of the location events. This allows tuning the determination of the substance within the fluid to a desired property range, which can relate to a desired kind of bound particles, thereby reducing the influence of unwanted effects on the determination of the substance and, thus, improving the determination accuracy.

公开(公告)号：US20130088221A1

公开(公告)日：2013-04-11

申请号：US13705000

申请日：2010-11-30

申请人： Joannes Baptist Adrianus Dionisius Van Zon ,  Mkhail Mikhaylovich Ovsyanko ,  Toon Hendrik Evers ,  Mara Johanna Jacoba Sijbers

发明人： Joannes Baptist Adrianus Dionisius Van Zon ,  Mkhail Mikhaylovich Ovsyanko ,  Toon Hendrik Evers ,  Mara Johanna Jacoba Sijbers

IPC分类号： G01N27/72

CPC分类号： G01N27/72 ,  B01L3/502761 ,  B82Y25/00 ,  G01N15/06 ,  G01N15/0656 ,  G01N2015/0693 ,  G01R33/1276 ,  H01F1/0036

摘要： The invention relates to a method and associated apparatuses (100) for the detection of magnetic particles (MP) in a sample chamber (111). The method comprises the determination of a “particle-parameter” that is related to the amount of magnetic particles (MP) in a first detection region (P, C), the determination of a “cluster-parameter” that is related to the degree of clustering of magnetic particles (MP) in a second detection region (P, C), and the evaluation of the particle-parameter based on the cluster-parameter. Various apparatuses are disclosed that can be applied in said method. In one apparatus (100), a magnetic field (B) is generated in the sample chamber (111) in such a way that it has different inclinations in a first and second field region (P, C) and/or that it is oblique to the binding surface (112) in at least one field region. Magnetic particles (MP) are then detected in said first and second field region and/or in said at least one field region before and after a permanent switch-off of the inclined magnetic field. The resulting detection signals are related to each other to determine a cluster-parameter. In other embodiments, a cluster-parameter may be determined from light transmission measurements during the application of a magnetic field that is switched on and off.

摘要翻译： 本发明涉及一种用于检测样品室（111）中的磁性颗粒（MP）的方法和相关装置（100）。 该方法包括确定与第一检测区域（P，C）中的磁性粒子（MP）的量相关的粒子参数，确定与磁性聚类程度相关的聚类参数 颗粒（MP）在第二检测区域（P，C）中，以及基于聚类参数的粒子参数的评估。 公开了可以应用于所述方法的各种装置。 在一个装置（100）中，在样品室（111）中产生磁场（B），使得其在第一和第二场区域（P，C）中具有不同的倾斜度和/或倾斜 到达至少一个场区域中的结合表面（112）。 然后在永久关闭倾斜磁场之前和之后，在所述第一和第二场区和/或所述至少一个场区中检测磁性颗粒（MP）。 所得到的检测信号彼此相关，以确定簇参数。 在其他实施例中，可以在施加接通和关断的磁场期间的光透射测量来确定簇参数。

公开(公告)号：US20110244596A1

公开(公告)日：2011-10-06

申请号：US13124426

申请日：2009-10-06

申请人： Toon Hendrik Evers ,  Mikhail Mikhaylovich Ovsyanko

发明人： Toon Hendrik Evers ,  Mikhail Mikhaylovich Ovsyanko

IPC分类号： G01N33/543 ,  G01N27/72

摘要： A method for controlling the movement of magnetic or magnetizable objects (10) in a biosensor cartridge. The method comprises the step of providing a biosensor cartridge with a laterally extending sensor surface (A) and at least a magnetic field generating means (20, 30, 30′) for generating a magnetic field with a field gradient substantially perpendicular to the sensor surface (A). The magnetic field generating means (20, 30, 30′) are alternatingly actuated such that the generated magnetic field directs alternatingly the magnetic or magnetizable objects (10) substantially perpendicular to the sensor surface (A) away and toward the sensor surface, wherein pulse lengths of the alternating actuation are adjusted such that a lateral movement of magnetizable objects along the laterally extending sensor surface is substantially avoided.

摘要翻译： 一种用于控制生物传感器盒中的磁性或可磁化物体（10）的运动的方法。 该方法包括以下步骤：向生物传感器盒提供横向延伸的传感器表面（A）和至少一个用于产生具有基本上垂直于传感器表面的场梯度的磁场的磁场产生装置（20,30,30'） （一个）。 磁场产生装置（20,30,30'）被交替地致动，使得所产生的磁场交替地引导基本上垂直于传感器表面（A）的磁性或可磁化物体（10）远离传感器表面，其中脉冲 调整交替致动的长度，使得可沿着横向延伸的传感器表面的可磁化物体的横向移动被基本上避免。

公开(公告)号：US10794903B2

公开(公告)日：2020-10-06

申请号：US13124426

申请日：2009-10-06

申请人： Toon Hendrik Evers ,  Mikhail Mikhaylovich Ovsyanko

发明人： Toon Hendrik Evers ,  Mikhail Mikhaylovich Ovsyanko

IPC分类号： G01N33/543 ,  B03C5/02 ,  B03C1/28 ,  G01N27/74 ,  B01L3/00 ,  G01R33/12

摘要： A method for controlling the movement of magnetic or magnetizable objects (10) in a biosensor cartridge. The method comprises the step of providing a biosensor cartridge with a laterally extending sensor surface (A) and at least a magnetic field generating means (20, 30, 30′) for generating a magnetic field with a field gradient substantially perpendicular to the sensor surface (A). The magnetic field generating means (20, 30, 30′) are alternatingly actuated such that the generated magnetic field directs alternatingly the magnetic or magnetizable objects (10) substantially perpendicular to the sensor surface (A) away and toward the sensor surface, wherein pulse lengths of the alternating actuation are adjusted such that a lateral movement of magnetizable objects along the laterally extending sensor surface is substantially avoided.

公开(公告)号：US20110207229A1

公开(公告)日：2011-08-25

申请号：US13124407

申请日：2009-10-06

申请人： Toon Hendrik Evers ,  Wendy Uyen Dittmer

发明人： Toon Hendrik Evers ,  Wendy Uyen Dittmer

IPC分类号： G01N33/00

CPC分类号： G01N33/54326 ,  B82Y25/00 ,  G01N27/745 ,  G01N35/0098 ,  G01R33/093 ,  G01R33/1269

摘要： The invention relates to a method and a device (100) for determining the amount of a target component (2) in a sample, wherein magnetic particles (2) can bind to a contact surface (4) with kinetics that depend on the sample-amount of the target component. The method comprises at least two washing steps during which magnetic particles (2) are magnetically moved away from the contact surface (4) and corresponding measurements of the remaining amount of magnetic particles (2) at the contact surface (4). The amount of target component in the sample is estimated from at least one of such measurement results. The measurement allows to determine also high concentrations of target component for which the sensor surface (4) is saturated in a steady-state.

摘要翻译： 本发明涉及一种用于确定样品中目标组分（2）的量的方法和装置（100），其中磁性颗粒（2）可以结合取决于样品的动力学的接触表面（4） 目标成分的数量。 该方法包括至少两个洗涤步骤，在此过程中，磁性颗粒（2）被磁性地远离接触表面（4）移动，并且在接触表面（4）处磁性颗粒（2）剩余量的相应测量值。 从这些测量结果中的至少一个估计样品中的目标组分的量。 该测量允许确定传感器表面（4）在稳定状态下饱和的目标部件的高浓度。

公开(公告)号：US20120171781A1

公开(公告)日：2012-07-05

申请号：US13496023

申请日：2010-09-07

申请人： Wendy Uyen Dittmer ,  Toon Hendrik Evers ,  Marco Hendrikus Hefti ,  David Walterus Cornelis Dekkers ,  Michael Franciscus Wilhelmus Cornelis Martens

发明人： Wendy Uyen Dittmer ,  Toon Hendrik Evers ,  Marco Hendrikus Hefti ,  David Walterus Cornelis Dekkers ,  Michael Franciscus Wilhelmus Cornelis Martens

IPC分类号： G01N33/566 ,  G01N21/75 ,  G01N33/577

CPC分类号： G01N33/54346 ,  G01N33/54333 ,  G01N2333/58

摘要： The present invention is related to an immunoassay for the detection of an analyte in a sample, said assay comprising a plurality of moieties capable of binding to said analyte, wherein capture moieties which are not specific for the same epitope are bound to a solid substrate, and at least one epitope-specific detection moiety is bound to a detectable marker, and wherein the detectable marker to which the epitope-specific detection moiety is bound is a large particle marker having a particle size of ≧50 nm and ≦5000 nm.

摘要翻译： 本发明涉及用于检测样品中分析物的免疫测定法，所述测定法包括能够结合所述分析物的多个部分，其中对相同表位不特异的捕获部分结合到固体底物上， 并且至少一个表位特异性检测部分结合到可检测标记上，并且其中表位特异性检测部分结合的可检测标记是具有≥50nm和＆lt; NE; 5000nm的粒度的大颗粒标记物。

公开(公告)号：US09720003B2

公开(公告)日：2017-08-01

申请号：US13141439

申请日：2009-12-15

申请人： Wendy Uyen Dittmer ,  Toon Hendrik Evers ,  Peggy De Kievit ,  Ricky Kamps ,  Joost Lambert Max Vissers ,  Michael Franciscus Wilhelmus Cornelis Martens ,  David Walterus Cornelis Dekkers

发明人： Wendy Uyen Dittmer ,  Toon Hendrik Evers ,  Peggy De Kievit ,  Ricky Kamps ,  Joost Lambert Max Vissers ,  Michael Franciscus Wilhelmus Cornelis Martens ,  David Walterus Cornelis Dekkers

IPC分类号： G01N33/68 ,  G01N33/543

CPC分类号： G01N33/6887 ,  G01N33/54373 ,  G01N2800/324

摘要： The present invention relates to a method for measuring Troponin I in a sample comprising the steps of providing a sample, contacting the sample with a monoclonal anti-Troponin I antibody coupled to a magnetic label, contacting the sample with a polyclonal anti-Troponin I antibody coupled to a sensor surface and detecting the magnetic label on the sensor surface. The invention further relates to a device and a cartridge for measuring Troponin I in a sample.

公开(公告)号：US09023651B2

公开(公告)日：2015-05-05

申请号：US13124407

申请日：2009-10-06

申请人： Toon Hendrik Evers ,  Wendy Uyen Dittmer

发明人： Toon Hendrik Evers ,  Wendy Uyen Dittmer

IPC分类号： G01N33/553 ,  G01N33/543 ,  B82Y25/00 ,  G01N27/74 ,  G01R33/09 ,  G01R33/12 ,  G01N35/00

CPC分类号： G01N33/54326 ,  B82Y25/00 ,  G01N27/745 ,  G01N35/0098 ,  G01R33/093 ,  G01R33/1269

摘要： The invention relates to a method and a device (100) for determining the amount of a target component (2) in a sample, wherein magnetic particles (2) can bind to a contact surface (4) with kinetics that depend on the sample-amount of the target component. The method comprises at least two washing steps during which magnetic particles (2) are magnetically moved away from the contact surface (4) and corresponding measurements of the remaining amount of magnetic particles (2) at the contact surface (4). The amount of target component in the sample is estimated from at least one of such measurement results. The measurement allows to determine also high concentrations of target component for which the sensor surface (4) is saturated in a steady-state.

摘要翻译： 本发明涉及一种用于确定样品中目标组分（2）的量的方法和装置（100），其中磁性颗粒（2）可以结合取决于样品的动力学的接触表面（4） 目标成分的数量。 该方法包括至少两个洗涤步骤，在此过程中，磁性颗粒（2）被磁性地远离接触表面（4）移动，并且在接触表面（4）处磁性颗粒（2）剩余量的相应测量值。 从这些测量结果中的至少一个估计样品中的目标组分的量。 该测量允许确定传感器表面（4）在稳定状态下饱和的目标部件的高浓度。

公开(公告)号：US11493507B2

公开(公告)日：2022-11-08

申请号：US13496023

申请日：2010-09-07

申请人： Wendy Uyen Dittmer ,  Toon Hendrik Evers ,  Marco Hendrikus Hefti ,  David Walterus Cornelis Dekkers ,  Michael Franciscus Wilhelmus Cornelis Martens

发明人： Wendy Uyen Dittmer ,  Toon Hendrik Evers ,  Marco Hendrikus Hefti ,  David Walterus Cornelis Dekkers ,  Michael Franciscus Wilhelmus Cornelis Martens

IPC分类号： G01N33/543

摘要： An immunoassay for the detection of an analyte in a sample includes a plurality of moieties capable of binding to the analyte. Capture moieties, which are not specific for the same epitope, are bound to a solid substrate, and at least one epitope-specific detection moiety is bound to a detectable marker. The detectable marker is a large particle marker having a particle size of ≥50 nm and ≤5000 nm.

公开(公告)号：US20120258553A1

公开(公告)日：2012-10-11

申请号：US13518428

申请日：2010-12-16

申请人： Wendy Uyen Dittmer ,  Toon Hendrik Evers ,  David Walterus Cornelis Dekkers ,  Marco Hendrikus Hefti ,  Joost Lambert Max Vissers ,  Michael Franciscus Wilhelmus Cornelis Martens

发明人： Wendy Uyen Dittmer ,  Toon Hendrik Evers ,  David Walterus Cornelis Dekkers ,  Marco Hendrikus Hefti ,  Joost Lambert Max Vissers ,  Michael Franciscus Wilhelmus Cornelis Martens

IPC分类号： G01N27/72

CPC分类号： G01N33/54333 ,  G01N21/552 ,  G01N27/745 ,  G01N33/54393 ,  G01N2446/00

摘要： An apparatus for measuring a target molecule in a sample is disclosed. The apparatus comprises a moiety comprising a magnetic label (1) greater than 100 nm, a binding surface (12) for specifically binding the moiety, the amount of said moiety binding to said surface being indicative of the amount of the target molecule in said sample, detection means (31 31′) for detecting the amount of said moiety bound to said surface, and a salt (51) for reducing aggregation of the magnetic labels of respective moieties in said sample. The apparatus preferably also comprises a magnetic field generator (41) for attracting the magnetic labels to the binding surface. A method for measuring a target molecule in a sample and a disposable cartridge for use with the apparatus are also disclosed.

摘要翻译： 公开了一种用于测量样品中靶分子的装置。 该装置包括包含大于100nm的磁性标签（1）的部分，用于特异性结合该部分的结合表面（12），所述部分结合所述表面的量指示所述样品中靶分子的量 ，用于检测与所述表面结合的所述部分的量的检测装置（31 31'）和用于减少所述样品中各部分的磁性标记物的聚集的盐（51）。 该装置优选地还包括用于将磁性标签吸引到装订表面的磁场发生器（41）。 还公开了一种用于测量样品中的靶分子的方法和用于该设备的一次性盒。