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322 条记录 (耗时 0.046 秒)

    Method for synthesizing DNA
    13.
    发明授权
    Method for synthesizing DNA 有权
    DNA合成方法

    公开(公告)号：US07521178B1

    公开(公告)日：2009-04-21

    申请号：US09673884

    申请日：1999-04-21

    申请人： Kiyozo Asada ,  Takashi Uemori ,  Yoshimi Sato ,  Tomoko Fujita ,  Kazue Miyake ,  Osamu Takeda ,  Hiroyuki Mukai ,  Ikunoshin Kato

    发明人： Kiyozo Asada ,  Takashi Uemori ,  Yoshimi Sato ,  Tomoko Fujita ,  Kazue Miyake ,  Osamu Takeda ,  Hiroyuki Mukai ,  Ikunoshin Kato

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02

    CPC分类号： C12P19/34 ,  C12N9/1252 ,  C12N9/96 ,  C12Q1/6806 ,  Y10S435/975 ,  C12Q2521/107

    摘要： A DNA synthesis reaction-enhancer comprising at least one kind selected from the group consisting of acidic substances and cationic complexes; a DNA synthesis method in which during a DNA synthesis reaction a reaction is carried out in the presence of the above enhancer by using DNA polymerase; a DNA synthesis reaction composition comprising the above enhancer; a DNA synthesis reaction composition comprising two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity; a DNA synthesis method in which during a DNA synthesis reaction two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity are used; a kit for use in in vitro DNA synthesis, comprising two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity; and a kit for use in in vitro DNA synthesis, wherein the kit comprises the DNA synthesis reaction-enhancer and DNA polymerase. According to the present invention, DNA synthesis can be carried out at an efficiency more excellent as compared to conventional DNA synthesis reaction.

    摘要翻译： 一种DNA合成反应增强剂，其包含选自酸性物质和阳离子配合物中的至少一种; DNA合成方法，其中在DNA合成反应中，通过使用DNA聚合酶在上述增强剂的存在下进行反应; 包含上述增强剂的DNA合成反应组合物; 包含两种或更多种各自具有3'→5'核酸外切酶活性的DNA聚合酶的DNA合成反应组合物; 一种DNA合成方法，其中在DNA合成反应中使用两种或更多种具有3'→5'核酸外切酶活性的DNA聚合酶; 用于体外DNA合成的试剂盒，其包含两种或更多种各自具有3'→5'核酸外切酶活性的DNA聚合酶; 和用于体外DNA合成的试剂盒，其中试剂盒包含DNA合成反应增强剂和DNA聚合酶。 根据本发明，与常规DNA合成反应相比，可以以更优异的效果进行DNA合成。

    Method for synthesizing DNA
    14.
    发明授权
    Method for synthesizing DNA 失效
    DNA合成方法

    公开(公告)号：US06673578B1

    公开(公告)日：2004-01-06

    申请号：US09786684

    申请日：2001-05-22

    申请人： Takashi Uemori ,  Yoshimi Sato ,  Mariko Okawa ,  Tomoko Fujita ,  Kazue Miyake ,  Osamu Takeda ,  Hiroaki Sagawa ,  Michio Hagiya ,  Hiroyuki Mukai ,  Kiyozo Asada ,  Ikunoshin Kato

    发明人： Takashi Uemori ,  Yoshimi Sato ,  Mariko Okawa ,  Tomoko Fujita ,  Kazue Miyake ,  Osamu Takeda ,  Hiroaki Sagawa ,  Michio Hagiya ,  Hiroyuki Mukai ,  Kiyozo Asada ,  Ikunoshin Kato

    IPC分类号： C12P1934

    CPC分类号： C12N15/1003 ,  C12Q1/686 ,  C12Q2527/125

    摘要： A DNA synthesis method with a shortened time period required for DNA synthesis by polymerase chain reaction (PCR), characterized in that a DNA polymerase is used in an amount effective for providing more than 10 ng of amplified DNA fragments of about 2 kb per 50 &mgr;l of a reaction mixture, when PCR is carried out under the following conditions (A) and (B): (A) reaction mixture: 50 &mgr;l volume of a reaction mixture comprising DNA polymerase, 1 ng of genomic DNA from Escherichia coli, and 10 pmol each of primers Eco-1 and Eco-2 (nucleotide sequences of the primers Eco-1 and Eco-2 being shown in SEQ ID NOs: 10 and 11 of Sequence Listing, respectively); and having a composition suitable for the DNA polymerase; and (B) reaction conditions: 35 cycles of PCR, wherein one cycle consists of 99° C., 1 second-66° C., 7 seconds; a kit for DNA synthesis usable for the DNA synthesis method; and an article of manufacture of a PCR agent. According to the present invention, the procedures in the genetic engineering studies and industries involved with PCR can be speeded up.

    摘要翻译： 一种DNA合成方法，其通过聚合酶链式反应（PCR）进行DNA合成所需的时间缩短，其特征在于使用DNA聚合酶，其量有效提供超过10ng每50ul约2kb的扩增DNA片段 的反应混合物，当在以下条件（A）和（B）下进行PCR时：（A）反应混合物：将50体积的包含DNA聚合酶的反应混合物，1ng来自大肠杆菌的基因组DNA和10ng 各引物Eco-1和Eco-2（引物Eco-1和Eco-2的核苷酸序列分别示于序列表的SEQ ID NO：10和11中） 并具有适合于DNA聚合酶的组合物; 和（B）反应条件：35个循环的PCR，其中一个循环由99℃，1秒-66℃，7秒; 用于DNA合成方法的DNA合成试剂盒; 以及PCR试剂的制造。 根据本发明，可以加快基因工程研究和涉及PCR的行业的程序。

    DNA polymerase-related factors
    18.
    发明授权
    DNA polymerase-related factors 有权
    DNA聚合酶相关因素

    公开(公告)号：US06333158B1

    公开(公告)日：2001-12-25

    申请号：US09712266

    申请日：2000-11-15

    申请人： Takashi Uemori ,  Yoshimi Sato ,  Tomoko Fujita ,  Kazue Miyake ,  Hiroyuki Mukai ,  Kiyozo Asada ,  Ikunoshin Kato

    发明人： Takashi Uemori ,  Yoshimi Sato ,  Tomoko Fujita ,  Kazue Miyake ,  Hiroyuki Mukai ,  Kiyozo Asada ,  Ikunoshin Kato

    IPC分类号： C12Q168

    CPC分类号： C12N9/1252

    摘要： The present invention relates to a thermostable DNA polymerase-associated factor capable of enhancing DNA synthesizing-activity of a DNA polymerase; a thermostable DNA polymerase-associated factor possessing an activity of binding to a DNA polymerase and a method for producing the same; a gene encoding the DNA polymerase-associated factor; a method of DNA synthesis by using a DNA polymerase in the presence of the DNA polymerase-associated factor; and a kit comprising the DNA polymerase-associated factor. According to the present invention, there can be provided in vitro DNA synthesis and a DNA amplification system which are more excellent than conventional techniques by utilizing the DNA polymerase-associated factor of the present invention.

    摘要翻译： 本发明涉及能够增强DNA聚合酶的DNA合成活性的热稳定的DNA聚合酶相关因子; 具有与DNA聚合酶结合的活性的热稳定DNA聚合酶相关因子及其制备方法; 编码DNA聚合酶相关因子的基因; 在DNA聚合酶相关因子存在下使用DNA聚合酶进行DNA合成的方法; 以及包含DNA聚合酶相关因子的试剂盒。 根据本发明，通过利用本发明的DNA聚合酶相关因子，可以提供比常规技术更优异的体外DNA合成和DNA扩增系统。