公开(公告)号：US08877459B2

公开(公告)日：2014-11-04

申请号：US13786498

申请日：2013-03-06

申请人： Bruker Daltonik GmbH

发明人： Ulrich Weller

IPC分类号： C12Q1/04 ,  C12N1/00 ,  G01N33/569 ,  G01N33/68

CPC分类号： C12Q1/04 ,  G01N33/569 ,  G01N33/6851

摘要： Identification of infectious pathogens, particularly viruses, bacteria and other microorganisms is effected with a method whereby pathogens of acute infections can be identified, without first culturing them in external nutrient media, by mass spectrometric measurement of their protein profiles obtained from pathogens directly precipitated from body fluid into pellets by centrifuging. With this method, pathogens which cause acute infections can be identified in less than one hour.

摘要翻译： 感染性病原体，特别是病毒，细菌和其他微生物的鉴定是通过以下方法进行的：通过质谱法测定从身体直接沉淀的病原体获得的蛋白质图谱，可以鉴定出急性感染病原体，而无需先将其培养在外部营养培养基中 通过离心将液体转化成颗粒。 通过这种方法，可以在不到一小时的时间内鉴定引起急性感染的病原体。

公开(公告)号：US08835176B2

公开(公告)日：2014-09-16

申请号：US13910279

申请日：2013-06-05

申请人： Bruker Daltonik GmbH

发明人： Jens Boβmeyer ,  Jochen Franzen

IPC分类号： G01N30/72 ,  C12N1/06 ,  H01J49/04 ,  G01N27/62 ,  C12Q1/04 ,  G01N33/543 ,  G01N33/68

CPC分类号： G01N27/62 ,  C12Q1/04 ,  G01N33/5438 ,  G01N33/6848 ,  H01J49/0418

摘要： The invention relates to the cell disruption of microbes and the preparation of the microbe proteins for mass spectrometric analysis. The cells of microbes from microcolonies are disrupted by physical or chemical means directly on the nutrient medium. The released proteins are then transferred to sample supports by direct contact with their contact surfaces; electrophoresis can be used for assistance. Once the proteins are firmly adsorbed on the contact surfaces, they can be washed with water in order to remove substances which interfere with the ionization process. For analysis by matrix-assisted laser desorption (MALDI), the proteins are prepared on the contact surfaces of the sample supports with matrix substances to form MALDI samples; the sample supports are then introduced into a MALDI mass spectrometer for the acquisition of mass spectra. The microbes are identified by similarity comparisons between the mass spectra of the microbe proteins and similarly obtained reference spectra.

摘要翻译： 本发明涉及微生物的细胞破碎和用于质谱分析的微生物蛋白质的制备。 来自微小菌落的微生物的细胞通过物理或化学方式直接在营养培养基上破坏。 然后将释放的蛋白质通过与其接触表面直接接触而转移到样品支架中; 电泳可用于协助。 一旦蛋白质牢固地吸附在接触表面上，它们可以用水洗涤，以去除干扰电离过程的物质。 为了通过基质辅助激光解吸（MALDI）分析，蛋白质在样品载体与基质物质的接触表面上制备形成MALDI样品; 然后将样品载体引入MALDI质谱仪中以获取质谱。 微生物通过微生物蛋白的质谱和类似获得的参考光谱之间的相似性比较来鉴定。

公开(公告)号：US08766176B2

公开(公告)日：2014-07-01

申请号：US13094102

申请日：2011-04-26

申请人： Melvin Andrew Park ,  Desmond Allen Kaplan ,  Mark Ridgeway

发明人： Melvin Andrew Park ,  Desmond Allen Kaplan ,  Mark Ridgeway

IPC分类号： H01J49/26

CPC分类号： G01N27/626

摘要： In an ion mobility spectrometer in which a gas flows through a gas-tight tube with a radially quadrupolar RF field therein and blows ions against a DC electric field barrier, a mobility scan with a mobility scale that is linear in time is obtained by holding the height of the DC electric field barrier constant while changing the pressure and temperature conditions of the flowing gas. Alternatively, the mobility scan is performed by holding the pressure and temperature conditions of the flowing gas constant and reducing the height of the DC electric field barrier non-linearly with respect to time.

摘要翻译： 在离子迁移谱仪中，气体通过其中具有径向四极RF场的气密管流动，并将离子吹向DC电场势垒，通过保持 直流电场势垒的高度在改变流动气体的压力和温度条件的同时。 或者，通过保持流动气体的压力和温度条件恒定并且相对于时间非线性地降低DC电场屏障的高度来执行移动扫描。

公开(公告)号：US08735812B2

公开(公告)日：2014-05-27

申请号：US13334191

申请日：2011-12-22

申请人： Claus Köster

发明人： Claus Köster

IPC分类号： H01J49/26 ,  H01R43/00

CPC分类号： H01J49/425 ,  Y10T29/49117

摘要： An electrostatic Kingdon ion trap in which ions can oscillate harmonically in the longitudinal direction, decoupled from their motions in the transverse direction is formed from at least three inner electrodes located inside a hollow outer housing electrode. The inner surface of the housing electrode and the outer surfaces of the inner electrodes are formed so that when a potential is applied between the housing and the inner electrodes, the potential distribution inside the housing contains not only a term for a harmonic potential well in the axial direction, but also a term for the potential distribution in the radial direction, that contains, independent of the axial coordinate, the equations for a family of Cassini curves of at least the third order.

摘要翻译： 其中离子可以在纵向方向上和谐地振荡的静电Kingdon离子阱，其从横向方向上的运动分离而形成，该至少三个内部电极位于空心的外部壳体电极内部。 壳体电极的内表面和内部电极的外表面形成为当壳体和内部电极之间施加电位时，壳体内部的电位分布不仅包含谐波势阱的项， 轴向方向，也是径向方向上的电位分布的项，其独立于轴向坐标，包含至少三阶卡西尼曲线族的方程式。

公开(公告)号：US08704173B2

公开(公告)日：2014-04-22

申请号：US13499817

申请日：2010-09-17

申请人： Evgenij Nikolaev ,  Ivan Boldin ,  Jochen Franzen

发明人： Evgenij Nikolaev ,  Ivan Boldin ,  Jochen Franzen

IPC分类号： H01J49/20 ,  H01J49/26 ,  H01J49/28

CPC分类号： H01J49/38

摘要： Devices and methods for the acquisition of mass spectra with very high mass resolution in ion cyclotron resonance mass spectrometers include cylindrical ICR measuring cells with special electrode geometries to generate harmonic trapping potentials for orbiting ions. The sheath of the cylindrical cell is divided by longitudinal gaps into a multitude of sheath electrodes, which either have to carry layers with resistance profiles able to generate parabolic voltage profiles along the sheath electrodes, or which form sheath electrodes of varying width by parabolic gaps. Orbiting ions of a given mass m/z oscillate harmonically in an axial direction with the same frequency, independent of the radius of their orbit and their oscillation amplitude. Ideally, the cylinders are closed by endcaps with rotationally hyperbolic form, divided into partial electrodes. The ions are excited by dipolar excitation fields. The orbiting ion clouds are kept together for much longer periods than was possible hitherto.

摘要翻译： 用于在离子回旋共振质谱仪中以非常高的质量分辨率采集质谱的装置和方法包括具有特殊电极几何形状的圆柱形ICR测量单元，以产生轨道离子的谐波陷阱电位。 圆柱形电池的护套由纵向间隙分成多个护套电极，这些护套电极必须承载具有能够沿着护套电极产生抛物线电压分布的电阻分布的层，或者通过抛物线间隙形成具有不同宽度的护套电极。 给定质量m / z的轨道离子在轴向方向上以相同的频率谐振振荡，与轨道的半径和振荡幅度无关。 理想情况下，气缸由具有旋转双曲线形式的端盖封闭，分成部分电极。 离子由偶极激发场激发。 轨道离子云保持在一段比以前更长的时间。

公开(公告)号：US20140106396A1

公开(公告)日：2014-04-17

申请号：US14050446

申请日：2013-10-10

申请人： BRUKER DALTONIK GMBH

发明人： Vadim Markovich GOVORUN ,  Jochen FRANZEN

IPC分类号： C12Q1/18

CPC分类号： G01N33/6851 ,  C12Q1/18

摘要： Microorganisms, particularly bacteria, are identified and characterized on the basis of a mass spectrometric measurement of their protein profiles with ionization by matrix-assisted laser desorption. In order to measure the microbial resistance to antibiotics, the protein profiles of microorganisms are measured after cultivation for a short time duration in nutrient media containing the antibiotics.

摘要翻译： 基于通过基质辅助激光解吸电离的蛋白质谱的质谱测定，鉴定和表征微生物，特别是细菌。 为了测量微生物对抗生素的抗性，微生物的蛋白质分布在含有抗生素的营养培养基中培养短时间后测量。

公开(公告)号：US08648298B2

公开(公告)日：2014-02-11

申请号：US12633421

申请日：2009-12-08

申请人： Jochen Franzen

发明人： Jochen Franzen

IPC分类号： H01J49/00

CPC分类号： H01J49/38

摘要： In an ion cyclotron resonance mass spectrometer ions are excited into cyclotron orbits by an alternating current excitation signal having a nonlinear function of the excitation frequency vs. time in a “chirp.” Such an excitation signal produces transients which have no pronounced beats, even if mixtures of many ion species, all having the same mass differences, are present. The dynamic measuring range for the image currents can thus be better utilized. In particular, sum spectra of specified quality can be generated from a significantly smaller number of individual transients, and thus in a significantly shorter measuring time.

摘要翻译： 在离子回旋共振质谱仪中，通过在“啁啾声”中具有激发频率对时间的非线性函数的交流激励信号将离子激发到回旋加速器轨道。 这种激发信号产生没有显着节拍的瞬变，即使存在所有具有相同质量差异的许多离子物质的混合物。 因此可以更好地利用图像电流的动态测量范围。 特别地，可以从显着较小数量的单个瞬变产生特定质量的和光谱，并且因此在显着较短的测量时间内产生。

公开(公告)号：US08624180B2

公开(公告)日：2014-01-07

申请号：US13094146

申请日：2011-04-26

申请人： Melvin Andrew Park ,  Jochen Franzen

发明人： Melvin Andrew Park ,  Jochen Franzen

IPC分类号： H01J49/00

CPC分类号： G01N27/626

摘要： In an ion mobility spectrometer in which a gas pushes ions along a spectrometer axis against and over an electrical field barrier, the electric field barrier is generated with a plateau of slightly increasing height along the axis of the spectrometer. Alternately, the electric filed barrier may have a plateau with constant height, but the gas flow decreases in velocity along the axis of the spectrometer in the vicinity of the plateau.

摘要翻译： 在离子迁移谱仪中，其中气体沿着光谱仪轴线推动离子抵抗和超过电场屏障，电场屏障是沿着光谱仪轴线的高度稍微增加的高原产生的。 或者，电场屏障可以具有恒定高度的平台，但气流在平台附近沿着光谱仪的轴线的速度减小。

公开(公告)号：US08605968B2

公开(公告)日：2013-12-10

申请号：US13028676

申请日：2011-02-16

申请人： Fedor Alexandrov

发明人： Fedor Alexandrov

IPC分类号： G06K9/00

CPC分类号： H01J49/0036 ,  G06K9/0014 ,  G06K9/6209 ,  G06T5/002 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/20076 ,  G06T2207/20192 ,  G06T2207/30024

摘要： Spatially resolved tissue states (status image) are determined from spectrally resolved mass spectra of a tissue section by (a) acquiring a plurality of spatially resolved mass spectra of the tissue section, (b) generating at least two mass images from the spatially resolved mass spectra, (c) smoothing the mass images using an edge-preserving smoothing algorithm and (d) calculating a status image from the smoothed mass images by means of a classification algorithm derived from mathematical statistics.

摘要翻译： 空间分辨的组织状态（状态图像）通过（a）获取组织部分的多个空间分辨质谱来确定组织切片的光谱解析质谱图，（b）从空间分辨质量产生至少两个质量图像 光谱，（c）使用边缘保持平滑算法平滑质量图像，以及（d）通过从数学统计导出的分类算法从平滑质量图像计算状态图像。

公开(公告)号：US08604426B2

公开(公告)日：2013-12-10

申请号：US13772701

申请日：2013-02-21

申请人： Bruker Daltonik GmbH

发明人： Ralf Hartmer

IPC分类号： H01J49/10 ,  G01N33/68 ,  B01D59/44

CPC分类号： G01N33/6848 ,  H01J49/0072

摘要： Radical anions for use in the fragmentation of positively charged biopolymer ions by means of electron transfer are produced from substances previously unknown for use as ETD production substances. The inventive substances produce radical anions that lead to electron transfer dissociations with a high yield of fragment ions. The substances have high volatility that allows them to be kept in unheated containers outside the vacuum system and transported into the vacuum system to an in vacuum electron attachment ion source via unheated lines and low molecular weights that allow the measurement of even very light fragment ions. In one embodiment, a suitable substance is 1-3-5-7-cyclooctatetraene.

摘要翻译： 用于通过电子转移使带正电荷的生物聚合物离子分解的自由基阴离子由以前未知的用作ETD生产物质的物质产生。 本发明的物质产生导致电子转移离解的自由基阴离子，具有高产率的碎片离子。 这些物质具有高挥发性，使其能够保持在真空系统外部的未加热的容器中，并通过未加热的管线和低分子量输送到真空电子附着离子源，允许测量甚至非常轻的碎片离子。 在一个实施方案中，合适的物质是1-3-5-7-环辛四烯。