METHODS FOR NEXT GENERATION GENOME WALKING AND RELATED COMPOSITIONS AND KITS

    公开(公告)号:US20210207207A1

    公开(公告)日:2021-07-08

    申请号:US17208538

    申请日:2021-03-22

    发明人: Brian Ward

    IPC分类号: C12Q1/6855 C12Q1/68 C40B20/04

    摘要: Methods are provided herein for identifying rare and/or unknown DNA sequences by next-generation sequencing approaches. Isolated double-stranded (ds), single-stranded (ss), or ds/ss DNA is fragmented and the fragments are polished, phosphorylated, and tailed, as necessary. Fragmentation can be enzymatic or mechanical. A universal adapter sequence is ligated to each fragment, wherein the adapter can have a top strand without a 5′ phosphate, a 3′ with an —H in place of the —OH, and/or a 3′ extra base complementary to any base added to the polished fragments. The ligatamers may then serve as templates for amplification using a forward primer complementary to the adapter sequence and a reverse primer targeted to the fragment sequence. Compositions produced by these methods and kits adapted for performing these methods are also described herein.

    EPIGENETIC MODIFICATION OF MAMMALIAN GENOMES USING TARGETED ENDONUCLEASES
    17.
    发明申请
    EPIGENETIC MODIFICATION OF MAMMALIAN GENOMES USING TARGETED ENDONUCLEASES 审中-公开
    使用靶向内含子的马马里兰基因的修饰

    公开(公告)号:US20170051354A1

    公开(公告)日:2017-02-23

    申请号:US15306720

    申请日:2015-04-24

    IPC分类号: C12Q1/68 C12N15/90

    摘要: The present disclosure provides genetically engineered cell lines comprising chromosomally integrated synthetic sequences having predetermined epigenetic modifications, wherein a predetermined epigenetic modification is correlated with a known diagnosis, prognosis or level of sensitivity to a disease treatment. Also provided are kits comprising said epigenetically modified synthetic nucleic acids or cells comprising said epigenetically modified synthetic nucleic acids that can be used as reference standards for predicting responsiveness to therapeutic treatments, diagnosing diseases, or predicting disease prognosis.

    摘要翻译: 本公开提供了包含具有预定表观遗传修饰的染色体整合的合成序列的遗传工程改造的细胞系,其中预定的表观遗传修饰与已知的疾病治疗的敏感性的诊断,预后或水平相关。 还提供了包含所述表观遗传修饰的合成核酸或包含所述表观遗传修饰的合成核酸的细胞的试剂盒,其可以用作预测对治疗性治疗的响应性,诊断疾病或预测疾病预后的参考标准。

    Molybdenum allyl complexes and use thereof in thin film deposition
    19.
    发明授权
    Molybdenum allyl complexes and use thereof in thin film deposition 有权
    钼烯丙基络合物及其在薄膜沉积中的应用

    公开(公告)号:US09175023B2

    公开(公告)日:2015-11-03

    申请号:US14374289

    申请日:2013-01-18

    摘要: Molybdenum complexes and use thereof in thin film deposition, such as CVD and ALD are provided herein. The molybdenum complexes correspond in structure to Formula (I) and Formula (II), wherein R1, R3, R5, R7, R8 and R10 are independently and at each occurrence alkyl; R2, R6 and R9 are independently alkyl; R4 and R11 are independently and at each occurrence selected from the group consisting of alkyl, alkenyl, and alkynyl; x, z, a, c, d and f are independently zero, 1, or 2; y, b and e are independently zero or 1; and n and m are independently zero to 5.

    摘要翻译: 钼复合物及其在薄膜沉积中的应用,如CVD和ALD。 钼络合物在结构上对应于式(I)和式(II),其中R 1,R 3,R 5,R 7,R 8和R 10独立地且在各处出现时为烷基; R2,R6和R9独立地是烷基; R 4和R 11独立地且每次出现时选自烷基,烯基和炔基; x,z,a,c,d和f分别为0,1或2; y,b和e独立为零或1; n和m分别为0〜5。

    TARGETED HISTONE ACETYLATION
    20.
    发明申请
    TARGETED HISTONE ACETYLATION 审中-公开
    有针对性的HISTONE乙酰化

    公开(公告)号:US20150079657A1

    公开(公告)日:2015-03-19

    申请号:US14381381

    申请日:2013-02-28

    IPC分类号: C12N9/10 C07K14/47

    摘要: The present disclosure provides compositions and methods for acetylating histones at targeted chromosomal locations in a cell. In particular, the disclosure provides a fusion protein comprising a DNA binding domain and at least one histone acetyltransferase (HAT) domain, such that the DNA binding domain targets the fusion protein to a targeted chromosomal location and the HAT domain acetylates histones at the targeted location.

    摘要翻译: 本公开提供了在细胞中靶向染色体位置处乙酰化组蛋白的组合物和方法。 具体地,本公开提供了包含DNA结合结构域和至少一种组蛋白乙酰转移酶(HAT)结构域的融合蛋白,使得DNA结合结构域将融合蛋白靶向靶向染色体位置,并且HAT结构域在靶位置乙酰化组蛋白 。