公开(公告)号：US5589466A

公开(公告)日：1996-12-31

申请号：US380131

申请日：1995-01-26

申请人： Philip L. Felgner ,  Jon A. Wolff ,  Gary H. Rhodes ,  Robert W. Malone ,  Dennis A. Carson

发明人： Philip L. Felgner ,  Jon A. Wolff ,  Gary H. Rhodes ,  Robert W. Malone ,  Dennis A. Carson

IPC分类号： A61K9/127 ,  A61K48/00 ,  C07K14/16 ,  C07K14/61 ,  C12N9/02 ,  C12N9/10 ,  C12N9/12 ,  C12N9/78 ,  C12N15/87 ,  C12N15/00

CPC分类号： C07K14/005 ,  A61K48/00 ,  A61K9/1272 ,  C07K14/61 ,  C12N15/87 ,  C12N9/0069 ,  C12N9/1033 ,  C12N9/1247 ,  C12N9/78 ,  C12Y113/12007 ,  C12N2740/16122

摘要： A method for delivering an isolated polynucleotide such as DNA or RNA, to the interior of a cell in a mammal comprising the injection of an isolated polynucleotide into a muscle of the mammal where the polynucleotide is taken up by the cells of the muscle and exerts a therapeutic effect on the mammal. The method can be used to deliver a therapeutic polypeptide to the cells of the mammal, to provide an immune response upon in vivo translation of the polynucleotide, to deliver antisense polynucleotides, to deliver receptors to the cells of the mammal or to provide transitory gene therapy.

摘要翻译： 用于将分离的多核苷酸（例如DNA或RNA）递送到哺乳动物细胞内部的方法，包括将分离的多核苷酸注射到哺乳动物的肌肉中，其中所述多核苷酸被肌肉的细胞吸收，并施加 对哺乳动物的治疗作用。 该方法可用于向哺乳动物的细胞递送治疗性多肽，以在多核苷酸的体内翻译中提供免疫应答，递送反义多核苷酸，将受体递送至哺乳动物的细胞或提供短暂的基因治疗 。

公开(公告)号：US5578465A

公开(公告)日：1996-11-26

申请号：US460412

申请日：1995-06-02

申请人： Brian W. Grinnell

发明人： Brian W. Grinnell

IPC分类号： C12N1/38 ,  C12N9/10 ,  C12N9/64 ,  C12N9/72 ,  C12N15/85 ,  C12P21/00 ,  C12P21/02

CPC分类号： C12N9/6464 ,  C12N1/38 ,  C12N15/85 ,  C12N9/1033 ,  C12N9/6459 ,  C12Y304/21069 ,  C12N2799/022 ,  C12N2830/00 ,  C12N2830/002 ,  C12N2830/30 ,  C12N2830/42 ,  C12N2830/55 ,  C12N2830/60 ,  C12N2830/85 ,  C12N2840/105 ,  C12N2840/20

摘要： The present invention is a method of using the BK enhancer in tandem with a eukaryotic promoter to promote transcription of DNA that encodes a useful substance. The method of the present invention requires the presence of the E1A gene product for maximum expression of the useful substance. The present invention also comprises a number of useful expression vectors that comprise the BK enhancer in tandem with the adenovirus 2 late promoter positioned to drive expression of a variety of proteins, such as protein C, chloramphenicol acetyltransferase, and tissue plasminogen activator. The present invention further comprises a method for increasing the activity of the BK enhancer involving placement of the BK enhancer immediately upstream of the eukaryotic promoter used in tandem with the BK enhancer to drive expression of a useful substance. Furthermore, the present invention also comprises a method for coamplification of genes in primate cells. Additionally, the invention further comprises the recombinant human protein C molecule produced in 293 cells which comprises novel glycosylation patterns.

公开(公告)号：US08367073B2

公开(公告)日：2013-02-05

申请号：US13069592

申请日：2011-03-23

申请人： Andrew R. Mendelsohn ,  Keith N. Haffer ,  James Larrick

发明人： Andrew R. Mendelsohn ,  Keith N. Haffer ,  James Larrick

IPC分类号： A61K39/00

CPC分类号： C07K14/655 ,  A23K50/60 ,  A61K38/31 ,  A61K39/0005 ,  A61K2039/552 ,  A61K2039/55555 ,  A61K2039/55566 ,  C07K2319/00 ,  C12N9/1033 ,  C12Y203/01028

摘要： Chimeric somatostatin-based polypeptides, polynucleotides used to encode the polypeptides, the methods for isolating and producing the polypeptides and the uses thereof are provided. In addition, low cost adjuvants for enhanced immunogenic response are provided. Vaccinations that include both chimeric somatostatin-based polypeptides and novel adjuvants are included, useful in facilitating farm animal productivity.

摘要翻译： 提供基于生物抑素的嵌段多肽，用于编码多肽的多核苷酸，分离和产生多肽的方法及其用途。 此外，提供了用于增强免疫原性应答的低成本佐剂。 包括嵌合生长抑素基多肽和新型佐剂两者的疫苗接种，可用于促进农场动物生产力。

公开(公告)号：US08143476B2

公开(公告)日：2012-03-27

申请号：US12470509

申请日：2009-05-22

申请人： Knut Meyer ,  Kevin L. Stecca

发明人： Knut Meyer ,  Kevin L. Stecca

IPC分类号： A01H1/00 ,  A01H5/00 ,  A01H5/10 ,  C12N15/05

CPC分类号： C12N15/8247 ,  A23L11/07 ,  A23V2002/00 ,  C12N9/1029 ,  C12N9/1033 ,  C12N9/1037

摘要： Transgenic soybean seed having increased total fatty acid content of at least 20% and altered fatty acid profiles when compared to the total fatty acid content of non-transgenic, null segregant soybean seed are described. DGAT genes from Yarrowia lipolytica combined with plastidic phosphoglucomutase down regulation are used to achieve the increase in seed storage lipids.

摘要翻译： 描述了与非转基因，无分离大豆种子的总脂肪酸含量相比，具有至少20％的总脂肪酸含量增加和改变的脂肪酸谱的转基因大豆种子。 使用解脂耶氏酵母的DGAT基因与质体磷酸葡萄糖转运酶下调相结合，实现种子储存脂质的增加。

公开(公告)号：US08039264B2

公开(公告)日：2011-10-18

申请号：US12510995

申请日：2009-07-28

申请人： Roumen Bogoev ,  Joseph Amshey ,  George Hanson

发明人： Roumen Bogoev ,  Joseph Amshey ,  George Hanson

IPC分类号： G01N33/68

CPC分类号： C12Y302/01031 ,  C07K1/13 ,  C12N9/1033 ,  C12N9/2442 ,  C12Y302/01014 ,  G01N33/533 ,  Y10T436/105831 ,  Y10T436/13 ,  Y10T436/18 ,  Y10T436/25

摘要： Methods, compositions, and kits for labeling tetracysteine-tagged proteins with biarsenical fluorophores with increased specificity, including compositions, methods and kits particularly adapted for labeling of tetracysteine-tagged proteins to be resolved within an electrophoresis gel.

摘要翻译： 用于用具有增加的特异性的双缩氟荧光团标记四半胱氨酸标记的蛋白质的方法，组合物和试剂盒，包括组合物，方法和试剂盒，特别适用于在电泳凝胶内标记待分解的四半胱氨酸标记的蛋白质。

公开(公告)号：US07166298B2

公开(公告)日：2007-01-23

申请号：US10906246

申请日：2005-02-10

申请人： Joel A. Jessee ,  William G. Hearl

发明人： Joel A. Jessee ,  William G. Hearl

IPC分类号： A61K9/127

CPC分类号： C12N15/88 ,  A61K39/00 ,  A61K2039/53 ,  C07K16/00 ,  C07K16/40 ,  C12N9/1033

摘要： A method for immunization using genetic material is disclosed. Compositions for genetic immunization comprising cationic lipids and polynucleotides are also disclosed. Methods for using genetic immunization to produce polyclonal and monoclonal antibodies are also disclosed. A method for epitope mapping is also disclosed.

摘要翻译： 公开了使用遗传材料进行免疫的方法。 还公开了包含阳离子脂质和多核苷酸的遗传免疫的组合物。 还公开了使用遗传免疫来产生多克隆和单克隆抗体的方法。 还公开了表位作图的方法。

公开(公告)号：US07045136B1

公开(公告)日：2006-05-16

申请号：US08470359

申请日：1995-06-06

申请人： Bernard Moss ,  Michael Mackett ,  Geoffrey L. Smith

发明人： Bernard Moss ,  Michael Mackett ,  Geoffrey L. Smith

IPC分类号： A61K39/275 ,  A61K39/285

CPC分类号： C12N15/86 ,  A61K39/00 ,  A61K39/12 ,  A61K39/275 ,  A61K39/285 ,  A61K2039/5256 ,  C07K14/005 ,  C12N7/00 ,  C12N9/1033 ,  C12N2710/16622 ,  C12N2710/24122 ,  C12N2710/24134 ,  C12N2710/24143 ,  C12N2730/10122 ,  C12N2760/20234 ,  Y02A50/403 ,  Y02A50/464 ,  Y02A50/466

摘要： Recombinant poxviruses, such as vaccinia, are provided that comprises a segment comprised of (A) a first DNA sequence encoding a polypeptide that is foreign to poxvirus and (B) a poxvirus transcriptional regulatory sequence, wherein (i) said transcriptional regulatory sequence is adjacent to and exerts transcriptional control over said first DNA sequence and (ii) said segment is positioned within a nonessential genomic region of said recombinant poxvirus. Vaccines, carriers, cells, and media comprising recombinant poxviruses, and methods of immunization with recombinant poxviruses also are provided.

摘要翻译： 提供重组痘病毒，例如牛痘，其包含由（A）编码痘病毒异源的多肽的第一DNA序列和（B）痘病毒转录调节序列组成的片段，其中（i）所述转录调控序列相邻 对所述第一DNA序列进行转录控制，并且（ii）所述片段位于所述重组痘病毒的非必需基因组区域内。 还提供了疫苗，载体，细胞和包含重组痘病毒的培养基，以及用重组痘病毒免疫的方法。

公开(公告)号：US06710035B2

公开(公告)日：2004-03-23

申请号：US09452872

申请日：1999-12-02

申请人： Philip L. Felgner ,  Jon Asher Wolff ,  Gary H. Rhodes ,  Robert Wallace Malone ,  Dennis A. Carson

发明人： Philip L. Felgner ,  Jon Asher Wolff ,  Gary H. Rhodes ,  Robert Wallace Malone ,  Dennis A. Carson

IPC分类号： A61K3170

CPC分类号： C07K14/005 ,  A61K9/1272 ,  A61K48/00 ,  C07K14/61 ,  C12N9/0069 ,  C12N9/1033 ,  C12N9/1247 ,  C12N9/78 ,  C12N15/87 ,  C12N2740/16122 ,  C12Y113/12007

摘要： A method for delivering an isolated polynucleotide to the interior of a cell in a vertebrate, comprising the interstitial introduction of an isolated polynucleotide into a tissue of the vertebrate where the polynucleotide is taken up by the cells of the tissue and exerts a therapeutic effect on the vertebrate. The method can be used to deliver a therapeutic polypeptide to the cells of the vertebrate, to provide an immune response upon in vivo translation of the polynucleotide, to deliver antisense polynucleotides, to deliver receptors to the cells of the vertebrate, or to provide transitory gene therapy.

摘要翻译： 一种用于将分离的多核苷酸递送至脊椎动物细胞内部的方法，包括将分离的多核苷酸间插入脊椎动物的组织中，其中所述多核苷酸被组织的细胞吸收并对其施用治疗效果 脊椎动物。 该方法可用于将治疗性多肽递送至脊椎动物的细胞，以在多核苷酸的体内翻译中提供免疫应答，递送反义多核苷酸，将受体递送至脊椎动物的细胞，或提供短暂基因 治疗。

公开(公告)号：US20020081660A1

公开(公告)日：2002-06-27

申请号：US09948815

申请日：2001-09-07

发明人： James R. Swartz ,  Dong-Myung Kim

IPC分类号： C12P021/02 ,  C12N001/21

CPC分类号： C12N9/1033 ,  C07K14/5255 ,  C12P21/00

摘要： Compositions and methods are provided for the enhanced in vitro synthesis of polypeptides. In order to improve the performance of in vitro protein synthesis reactions, metabolic inhibitors, or manipulation of a source organism, is used to diminish or avoid the action of enzymes responsible for undesirable amino acids production or depletion. A homeostatic system may be used for production of ATP, where the required high energy phosphate bonds are generated in situ, e.g. through coupling with an oxidation reaction. The homeostatic energy source will typically lack high energy phosphate bonds itself, and will therefore utilize free phosphate in the reaction mix during generation of ATP. The homeostatic energy source is provided in combination with an enzyme that catalyzes the creation of high energy phosphate bonds and with an enzyme that can use that high energy phosphate bond to regenerate ATP.

摘要翻译： 提供组合物和方法用于增强多肽的体外合成。 为了改善体外蛋白质合成反应的性能，使用代谢抑制剂或来源生物体的操作来减少或避免负责不良氨基酸产生或消耗的酶的作用。 可以使用稳态系统来生产ATP，其中所需的高能磷酸键是原位生成的。 通过与氧化反应的偶联。 稳态能源本身通常缺乏高能量磷酸键，因此在产生ATP期间将在反应混合物中利用游离磷酸盐。 稳态能量源与催化高能磷酸键的酶的结合，以及能够使用高能磷酸键重新生成ATP的酶组合提供。

公开(公告)号：US20020023276A1

公开(公告)日：2002-02-21

申请号：US08229978

申请日：1994-04-19

发明人： JEFFREY M. ROSEN

IPC分类号： A01K067/027 ,  C12P021/02 ,  C12N015/63

CPC分类号： A01K67/0275 ,  A01K67/02 ,  A01K2217/05 ,  A01K2217/20 ,  A01K2227/10 ,  A01K2227/105 ,  A01K2267/01 ,  A23C9/20 ,  A23C2230/05 ,  C07K14/4732 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/61 ,  C12N9/1033 ,  C12N15/63 ,  C12N15/85 ,  C12N15/8509 ,  C12N15/89 ,  C12N2830/008 ,  C12N2830/30 ,  C12N2830/85

摘要： Described is a method of targeting specific genes to the mammary gland which results in the efficient synthesis and secretion of biologically important molecules. Further, there is described as a composition of matter, a transgenic mammal having the ability to reproduce itself and being suitable for the secretion of biologically active agents into its milk. Additionally there is disclosed as a composition of matter, recombinant DNA gene complexes designed to integrate into a mammalian genome and to synthesize and secrete biological active agents into the milk. Furthermore methods of producing and using altered milk are disclosed.

摘要翻译： 描述了一种靶向乳腺特异性基因的方法，其导致生物重要分子的有效合成和分泌。 此外，描述为物质组合物，转基因哺乳动物具有自身繁殖并适于将生物活性剂分泌到其乳中的能力。 另外公开了一种物质组合物，设计用于整合到哺乳动物基因组中并将生物活性剂合成并分泌到乳中的重组DNA基因复合物。 另外公开了生产和使用改性牛奶的方法。