公开(公告)号：US08362216B2

公开(公告)日：2013-01-29

申请号：US13252034

申请日：2011-10-03

申请人： Philip A. Beachy ,  Jeffrey A. Porter ,  Randall T. Moon

发明人： Philip A. Beachy ,  Jeffrey A. Porter ,  Randall T. Moon

IPC分类号： C07K16/00 ,  C07K16/18 ,  C12P19/00 ,  A61K39/00

CPC分类号： G01N33/5008 ,  A01K67/0339 ,  A01K2217/05 ,  A01K2227/706 ,  A01K2267/03 ,  A61K38/00 ,  C07K14/43581 ,  C07K14/46 ,  C07K14/47 ,  C07K2319/00 ,  C07K2319/21 ,  C07K2319/23 ,  C07K2319/50 ,  C12N15/62 ,  G01N33/5011 ,  G01N33/5058 ,  G01N33/68 ,  G01N33/92 ,  G01N2333/43573 ,  G01N2333/4603 ,  G01N2333/4606 ,  G01N2333/465 ,  G01N2333/47 ,  G01N2500/10

摘要： The present invention provides two novel polypeptides, referred to as the “N” and “C” fragments of hedgehog, or N-terminal and C-terminal fragments, respectively, which are derived after specific cleavage at a G′CF site recognized by the autoproteolytic domain in the native protein. Also included are sterol-modified hedgehog polypeptides and functional fragments thereof. Methods of identifying compositions which affect hedgehog activity based on inhibition of cholesterol modification of hedgehog protein are described.

摘要翻译： 本发明提供了两种新的多肽，分别被称为刺猬蛋白的N和C片段，或N末端和C末端片段，其是在特异性切割后在自身蛋白水解结构域识别的G'CF位点衍生的 天然蛋白质 还包括固醇修饰的刺猬蛋白多肽及其功能片段。 描述了基于对刺猬蛋白胆固醇修饰的抑制影响刺猬蛋白活性的组合物的方法。

公开(公告)号：US20090186029A1

公开(公告)日：2009-07-23

申请号：US11804733

申请日：2007-05-18

申请人： Philip A. Beachy ,  Jeffrey A. Porter

发明人： Philip A. Beachy ,  Jeffrey A. Porter

IPC分类号： A61K39/395 ,  C07K16/00 ,  A61P43/00

CPC分类号： G01N33/5005 ,  A01K67/0339 ,  A01K2217/05 ,  A01K2227/706 ,  A01K2267/03 ,  A61K38/00 ,  C07K14/43581 ,  C07K14/46 ,  C07K14/47 ,  C07K2319/00 ,  C07K2319/21 ,  C07K2319/23 ,  C07K2319/50 ,  C12N15/62 ,  G01N33/5008 ,  G01N33/5011 ,  G01N33/502 ,  G01N33/5058 ,  G01N33/68 ,  G01N33/92 ,  G01N2333/43573 ,  G01N2333/4603 ,  G01N2333/4606 ,  G01N2333/465 ,  G01N2333/47 ,  G01N2500/10

摘要： The present invention provides two novel polypeptides, referred to as the “N” and “C” fragments of hedgehog, or N-terminal and C-terminal fragments, respectively, which are derived after specific cleavage at a G′CF site recognized by the autoproteolytic domain in the native protein. Also included are sterol-modified hedgehog polypeptides and functional fragments thereof. Methods of identifying compositions which affect hedgehog activity based on inhibition of cholesterol modification of hedgehog protein are described.

摘要翻译： 本发明提供了两种新的多肽，分别被称为刺猬蛋白的“N”和“C”片段，或N末端和C末端片段，其在由G'CF识别的G'CF位点上的特异性切割后衍生 天然蛋白质中的自体蛋白水解结构域。 还包括固醇修饰的刺猬蛋白多肽及其功能片段。 描述了基于对刺猬蛋白胆固醇修饰的抑制影响刺猬蛋白活性的组合物的方法。

公开(公告)号：US20030096319A1

公开(公告)日：2003-05-22

申请号：US10301245

申请日：2002-11-21

发明人： Patricia V. Phelps

IPC分类号： G01N033/53 ,  G01N033/567 ,  A01K031/19

CPC分类号： G01N33/5088 ,  A01K43/00 ,  G01N33/50 ,  G01N33/743 ,  G01N2333/465 ,  Y10S530/827 ,  Y10S530/852 ,  Y10S530/853 ,  Y10T436/10 ,  Y10T436/143333

摘要： A method of determining the gender of a bird in ovo comprises detecting the presence or absence of an elevated level of a sex-related hormone in the extra-embryonic fluid of the bird egg, and then determining the gender of the bird within the egg from the presence of an elevated level of a sex-related hormone therein. Preferably, the sex-related hormone is an estrogen. Further preferred are methods in which the extra-embryonic fluid is allantoic fluid. The method is preferably carried out on chicken eggs prior to or during transfer of the eggs from incubator to hatcher.

摘要翻译： 一种确定卵巢中鸟类性别的方法包括检测鸡蛋超胚胎液中存在或不存在高水平的性相关激素，然后确定鸡蛋内的鸟的性别 其中存在高水平的性别相关激素。 优选地，性别相关的激素是雌激素。 进一步优选的是胚胎液是尿囊液的方法。 该方法优选在鸡蛋从孵化器转移到孵化器之前或期间在鸡蛋上进行。

公开(公告)号：US5763206A

公开(公告)日：1998-06-09

申请号：US600417

申请日：1996-02-13

申请人： Roy H. Hammerstedt ,  Guy F. Barbato ,  Palmer G. Cramer

发明人： Roy H. Hammerstedt ,  Guy F. Barbato ,  Palmer G. Cramer

IPC分类号： A61D19/00 ,  C12M1/20 ,  C12M3/00 ,  C12M3/04 ,  G01N33/50 ,  G01N33/53 ,  G01N33/68 ,  C12Q1/02 ,  A61K35/54

CPC分类号： G01N33/689 ,  G01N33/5005 ,  G01N33/5091 ,  G01N2333/465 ,  G01N2800/367 ,  Y10S435/806

摘要： A method for testing the potential fertility of spermatozoa in a sample by contacting an aliquot of the sperm sample onto a protein extracted from native vitelline membranes removed from chicken or turkey eggs to enable quantification of the number of sperm which bind to the protein. Typically, the vitelline membrane protein is coated onto a solid substrate and the protein extract is first prepared by dissecting vitelline membranes (lamina perivitelline plus lamina extravitellina) from either a group of chicken eggs or a group of turkey eggs, rinsing the membranes free of albumin and yolk, and subdividing the membranes into small particles. The resulting protein is heat solubilized, purified with centrifugation and concentrated to yield the protein extract in solution. The extracted protein then is used as a protein substrate for spermatozoa binding, with sequential contacting of the protein extract with the spermatozoa and measuring the amount of sperm-egg binding that takes place. Intact egg membranes may alternatively be used to bind sperm. Correlation between sperm-egg binding and fertility of the spermatozoa in the sample is direct and linear.

摘要翻译： 通过将精子样本的等分试样接触到从鸡或火鸡卵中取出的天然卵黄素膜提取的蛋白质上来测试样品中精子的潜在生育力的方法，以便能够定量结合蛋白质的精子的数量。 通常，将卵黄蛋白膜蛋白质包被在固体基质上，蛋白质提取物首先通过从一组鸡蛋或一组火鸡卵中分离卵黄膜（薄层perivitelline plus lamina extravitellina）来制备，冲洗不含白蛋白的膜 和蛋黄，并将膜细分成小颗粒。 将所得蛋白质加热溶解，离心纯化并浓缩，得到溶液中的蛋白质提取物。 然后将提取的蛋白质用作精子结合的蛋白质底物，蛋白质提取物与精子连续接触并测量发生的精子 - 卵结合量。 还可以使用完整的卵膜来结合精子。 样本中精子结合与精子生育力的相关性是直接和线性的。

公开(公告)号：US20230160911A1

公开(公告)日：2023-05-25

申请号：US17048911

申请日：2019-04-02

申请人： Universiteit Gent

发明人： Filip Van Immerseel ,  Richard Ducatelle ,  Venessa Eeckhaut ,  Evy Goossens ,  Fien De Meyer

IPC分类号： G01N33/68

CPC分类号： G01N33/6893 ,  G01N2333/465 ,  G01N2800/06

摘要： Growth performance, health and welfare of domesticated birds such as broilers heavily depends on a well-functioning intestinal tract. Consequently, there is a high need to find biomarkers that are specific for gut damage and that are easily applicable in the field. The present disclosure describes a set of 20 specific proteins that can be quantified in fresh fecal droppings and/or intestinal content of the birds and that each specifically correlate with a damaged gut.

公开(公告)号：US09081025B2

公开(公告)日：2015-07-14

申请号：US13119078

申请日：2009-09-07

申请人： Giacomo Rossi ,  Stefano Pesaro

发明人： Giacomo Rossi ,  Stefano Pesaro

IPC分类号： G01N33/53 ,  G01N31/00 ,  G01N33/92

CPC分类号： G01N33/92 ,  G01N2333/465 ,  G01N2405/10 ,  G01N2800/06

摘要： A ganglioside or a mixture of gangliosides, isolated from the peripheral and central nervous system of a bird, in particular a parrot, their use for the preparation of a medicament, methods for the diagnosis of Proventricular Dilatation Disease and diagnostic kits thereof, are disclosed.

摘要翻译： 公开了从鸟的外周和中枢神经系统特别是鹦鹉分离的神经节苷脂或神经节苷脂的混合物，其用于制备药物的用途，诊断室性扩张症的方法及其诊断试剂盒。

公开(公告)号：US20100143890A1

公开(公告)日：2010-06-10

申请号：US12633131

申请日：2009-12-08

申请人： Hyobong HONG ,  Soohyung Lee ,  Taewan Kim ,  Myungae Chung ,  Sungwon Shon

发明人： Hyobong HONG ,  Soohyung Lee ,  Taewan Kim ,  Myungae Chung ,  Sungwon Shon

IPC分类号： C12Q1/70 ,  C12M1/34 ,  C07K14/00 ,  C07D235/02

CPC分类号： G01N33/56983 ,  C07D207/16 ,  C07D495/04 ,  G01N33/531 ,  G01N2333/11 ,  G01N2333/465

摘要： Disclosed herein are peptide, particularly dipeptide compounds and the application thereof to the detection or inhibition of AI virus. The peptide compounds are more stable and easier to synthesize and store than are antibodies. In addition, having strong binding forces for the H5 protein of AI virus, the peptide compounds are useful as capturers or inhibitors of AI virus.

摘要翻译： 本文公开了肽，特别是二肽化合物及其在检测或抑制AI病毒中的应用。 肽化合物比抗体更稳定，更易于合成和储存。 此外，对于AI病毒的H5蛋白具有强的结合力，肽化合物可用作AI病毒的捕获剂或抑制剂。

公开(公告)号：US06911528B1

公开(公告)日：2005-06-28

申请号：US09562914

申请日：2000-05-02

申请人： Philip A. Beachy ,  Jeffrey A. Porter

发明人： Philip A. Beachy ,  Jeffrey A. Porter

IPC分类号： A61K38/00 ,  C07K14/435 ,  C07K14/46 ,  C07K14/47 ,  C12N15/62 ,  G01N33/68 ,  G01N33/92 ,  C07K14/475

CPC分类号： G01N33/5005 ,  A01K67/0339 ,  A01K2217/05 ,  A01K2227/706 ,  A01K2267/03 ,  A61K38/00 ,  C07K14/43581 ,  C07K14/46 ,  C07K14/47 ,  C07K2319/00 ,  C07K2319/21 ,  C07K2319/23 ,  C07K2319/50 ,  C12N15/62 ,  G01N33/5008 ,  G01N33/5011 ,  G01N33/502 ,  G01N33/5058 ,  G01N33/68 ,  G01N33/92 ,  G01N2333/43573 ,  G01N2333/4603 ,  G01N2333/4606 ,  G01N2333/465 ,  G01N2333/47 ,  G01N2500/10

摘要： The present invention provides two novel polypeptides, referred to as the “N” and “C” fragments of hedgehog, or N-terminal and C-terminal fragments, respectively, which are derived after specific cleavage at a G↓CF site recognized by the autoproteolytic domain in the native protein. Also included are sterol-modified hedgehog polypeptides and functional fragments thereof. Methods of identifying compositions which affect hedgehog activity based on inhibition of cholesterol modification of hedgehog protein are described.

摘要翻译： 本发明提供两种新的多肽，分别被称为刺猬蛋白的“N”和“C”片段，或分别在由G↓CF位点识别的G↓CF位点上特异性切割后衍生的N-末端和C-末端片段 天然蛋白质中的自体蛋白水解结构域。 还包括固醇修饰的刺猬蛋白多肽及其功能片段。 描述了基于对刺猬蛋白胆固醇修饰的抑制影响刺猬蛋白活性的组合物的方法。

公开(公告)号：US06506570B1

公开(公告)日：2003-01-14

申请号：US09269366

申请日：1999-03-23

申请人： Patricia V. Phelps

发明人： Patricia V. Phelps

IPC分类号： C07K100

CPC分类号： G01N33/5088 ,  A01K43/00 ,  G01N33/50 ,  G01N33/743 ,  G01N2333/465 ,  Y10S530/827 ,  Y10S530/852 ,  Y10S530/853 ,  Y10T436/10 ,  Y10T436/143333

摘要： A method of determining the gender of a bird in ovo comprises detecting the presence or absence of an elevated level of a sex-related hormone in the extra-embryonic fluid of the bird egg, and then determining the gender of the bird within the egg from the presence of an elevated level of a sex-related hormone therein. Preferably, the sex-related hormone is an estrogen. Further preferred are methods in which the extra-embryonic fluid is allantoic fluid. The method is preferably carried out on chicken eggs prior to or during transfer of the eggs from incubator to hatcher.

摘要翻译： 一种确定卵巢中鸟类性别的方法包括检测鸡蛋超胚胎液中存在或不存在高水平的性相关激素，然后确定鸡蛋内的鸟的性别 其中存在高水平的性别相关激素。 优选地，性别相关的激素是雌激素。 进一步优选的是胚胎液是尿囊液的方法。 该方法优选在鸡蛋从孵化器转移到孵化器之前或期间在鸡蛋上进行。

公开(公告)号：US6022951A

公开(公告)日：2000-02-08

申请号：US628540

申请日：1996-04-10

申请人： Takeshi Sano ,  Charles R. Cantor ,  Sandor Vajda ,  Gabriel O. Reznik ,  Cassandra L. Smith ,  Mark W. Pandori

发明人： Takeshi Sano ,  Charles R. Cantor ,  Sandor Vajda ,  Gabriel O. Reznik ,  Cassandra L. Smith ,  Mark W. Pandori

IPC分类号： A61K38/00 ,  C07K14/36 ,  C12N1/21 ,  C12N15/31 ,  G01N33/538

CPC分类号： G01N33/538 ,  C07K14/36 ,  A61K38/00 ,  G01N2333/465 ,  Y10S530/808 ,  Y10S530/81

摘要： The present invention relates to streptavidin proteins and peptides having a altered physical properties such as an increased stability or increased or decreased affinity for binding biotin. The invention also relates to methods for the detection, identification, separation and isolation of targets using streptavidin proteins or peptides. Streptavidin with increased or reduced affinity allows for the use of the streptavidin-biotin coupling systems for detection and isolation systems wherein it is necessary to remove of one or the other of the binding partners. Such systems are useful for the purification of functional proteins and viable cells. The invention also relates to nucleic acids which encode these streptavidin proteins and peptides and to recombinant cells such as bacteria, yeast and mammalian cells which contain these nucleic acids.

摘要翻译： 本发明涉及链霉抗生物素蛋白和具有改变的物理性质例如增加的稳定性或增加或降低的结合生物素的亲和力的肽。 本发明还涉及使用链霉亲和素蛋白或肽检测，鉴定，分离和分离靶的方法。 具有增加或降低亲和力的链霉亲和素允许使用链霉抗生物素蛋白 - 生物素偶联系统进行检测和分离系统，其中必须除去一种或另一种结合配偶体。 这样的系统可用于纯化功能性蛋白质和活细胞。 本发明还涉及编码这些链霉抗生物素蛋白和肽以及含有这些核酸的重组细胞如细菌，酵母和哺乳动物细胞的核酸。