公开(公告)号：US20240027478A1

公开(公告)日：2024-01-25

申请号：US18256199

申请日：2021-12-06

申请人： Genzyme Corporation

发明人： Stacey Poloskey ,  Shauna Andersson ,  Pratik R. Bhagunde ,  Yue Cui ,  Sajida Iqbal ,  Baisong Mei

IPC分类号： G01N33/86 ,  A61K31/713 ,  A61P7/04

CPC分类号： G01N33/86 ,  A61K31/713 ,  A61P7/04 ,  G01N2333/8128 ,  G01N2800/224 ,  G01N2800/52

摘要： The present disclosure provides a method that aims to maintain a favorable benefit-risk balance for patients with hemophilia A or B with or without inhibitors treated with fitusiran.

公开(公告)号：US20160282344A1

公开(公告)日：2016-09-29

申请号：US15036805

申请日：2014-11-15

申请人： ASTUTE MEDICAL, INC. ,  UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION

发明人： Joseph Anderberg ,  Jeff Gray ,  Paul McPherson ,  Kevin Nakamura ,  James Patrick Kampf ,  Thomas Kwan ,  John A. Kellum ,  Derek C. Angus

IPC分类号： G01N33/569

CPC分类号： G01N33/56911 ,  G01N33/6893 ,  G01N2333/47 ,  G01N2333/4724 ,  G01N2333/745 ,  G01N2333/78 ,  G01N2333/8128 ,  G01N2333/96463 ,  G01N2800/26 ,  G01N2800/52

摘要： The present invention relates to methods and compositions for monitoring, diagnosis, prognosis, and determination of treatment regimens in sepsis patients and in patients at risk for sepsis. In particular, the invention relates to using assays that detect one or more biomarkers as diagnostic and prognostic biomarker assays in such patients.

公开(公告)号：US09388452B2

公开(公告)日：2016-07-12

申请号：US13638919

申请日：2011-04-08

申请人： Peter Matthiessen

发明人： Peter Matthiessen

IPC分类号： G01N33/48 ,  G01N33/50 ,  C12Q1/56 ,  G06F19/16 ,  G06F17/10 ,  G06F17/11 ,  G06F19/12 ,  C07K14/755 ,  C07K14/81 ,  G01N33/68

CPC分类号： C12Q1/56 ,  C07K14/755 ,  C07K14/8128 ,  G01N33/6803 ,  G01N2333/755 ,  G01N2333/8128 ,  G06F17/10 ,  G06F17/11 ,  G06F19/12 ,  G06F19/16

摘要： The present invention relates to a method for determining the highest temperature that is suitable for performing accelerated protein stability studies, as well as to a method for modeling real-time protein stability from accelerated stability data generated at said temperature.

摘要翻译： 本发明涉及一种用于确定适合进行加速蛋白质稳定性研究的最高温度的方法，以及用于通过在所述温度下产生的加速稳定性数据建模实时蛋白稳定性的方法。

公开(公告)号：US09329183B2

公开(公告)日：2016-05-03

申请号：US14710097

申请日：2015-05-12

申请人： Omrix Biopharmaceuticals Ltd.

发明人： Israel Nur ,  Nadav Orr ,  Roni Mintz

IPC分类号： G01N33/573 ,  G01N33/86 ,  C07K16/36

CPC分类号： G01N33/573 ,  C07K16/36 ,  G01N33/86 ,  G01N2333/8128 ,  G01N2333/815 ,  G01N2333/974

摘要： The invention relates to an in vitro immunoassay for quantifying thrombin in a sample comprising anti-thrombin III (AT-III) and thrombin. The method comprises the following steps: contacting the sample with a small molecule that recognizes the substrate binding site of thrombin; contacting the thrombin with a thrombin specific antibody raised against a thrombin blocked in the active site; and measuring the level of bound antibody.

公开(公告)号：US06506895B2

公开(公告)日：2003-01-14

申请号：US09028806

申请日：1998-02-24

申请人： Patrick E. Guire ,  Melvin J. Swanson ,  Gary W. Opperman

发明人： Patrick E. Guire ,  Melvin J. Swanson ,  Gary W. Opperman

IPC分类号： C07H2100

CPC分类号： C07H21/00 ,  A61L27/14 ,  A61L27/34 ,  A61L29/04 ,  A61L29/085 ,  A61L31/04 ,  A61L31/10 ,  A61L33/0011 ,  A61L33/0047 ,  C12N11/08 ,  C12Q1/56 ,  C12Q1/6834 ,  G01N33/543 ,  G01N33/68 ,  G01N33/6845 ,  G01N33/6854 ,  G01N2333/78 ,  G01N2333/8128 ,  G01N2333/815 ,  G01N2333/974

摘要： A photoactivatable nucleic acid derivative composition in which one or more photoreactive group(s) are bound to a natural or synthetic nucleic acid. The photoreactive groups can be bound to the nucleic acid before, during or after its formation, and can thereafter be activated in order to attach the nucleic acid to another molecule, e.g., to the surface of a solid support. Also described is a method of preparing such a composition, and a method of using such a composition to attach the nucleic acid to a another molecule, such as that provided by the surface of a substrate used to prepare a nucleic acid chip by photolithographic techniques.

摘要翻译： 一种可光活化的核酸衍生物组合物，其中一个或多个光反应基团与天然或合成核酸结合。 光反应性基团可以在其形成之前，期间或之后与核酸结合，然后可以活化以将核酸附着到另一个分子，例如固体支持物的表面。 还描述了制备这种组合物的方法，以及使用这种组合物将核酸附着到另一分子上的方法，例如由用于通过光刻技术制备核酸芯片的底物的表面提供的核酸。

公开(公告)号：US5891647A

公开(公告)日：1999-04-06

申请号：US727427

申请日：1996-10-11

申请人： Jean-Claude Lormeau ,  Renee Georgette Milleblandine ,  Francis Egiste Joseph Paolucci ,  Bernard Pau

发明人： Jean-Claude Lormeau ,  Renee Georgette Milleblandine ,  Francis Egiste Joseph Paolucci ,  Bernard Pau

IPC分类号： C07K16/38 ,  G01N33/86 ,  G01N33/53 ,  C07K16/00 ,  C12P21/06 ,  G01N33/543

CPC分类号： C07K16/38 ,  G01N33/86 ,  G01N2333/8128

摘要： A method that assays activated antithrombin III (AT III) is useful for monitoring the effect in vivo of an agent that activates AT III. It involves the steps of contacting AT III with a solution sample containing the agent, wherein the agent is a sulfated glycosaminoglycan or a sulfated oligosaccharide, followed by contacting the sample with i) a first monoclonal antibody that preferentially binds AT III activated by the agent, as opposed to binding native AT III, and ii) a second monoclonal antibody that preferentially binds denatured AT III as opposed to either AT III activated by the agent or native AT III.

摘要翻译： PCT No.PCT / FR95 / 00456 Sec。 371日期：1996年10月11日 102（e）日期1996年10月11日PCT提交1995年4月10日PCT公布。 出版物WO95 / 28644 日期1995年10月26日测定激活的抗凝血酶III（AT III）的方法可用于监测激活AT III的药物的体内效应。 它包括使AT III与含有试剂的溶液样品接触的步骤，其中所述试剂是硫酸化糖胺聚糖或硫酸化寡糖，然后使样品与i）优先结合被试剂活化的AT III的第一单克隆抗体， 与结合天然AT III相反，和ii）优先结合变性AT III的第二单克隆抗体，而不是由所述试剂或天然AT III活化的AT III。

公开(公告)号：US5753445A

公开(公告)日：1998-05-19

申请号：US233390

申请日：1994-04-26

申请人： Howard M. Fillit ,  Peter C. Harpel

发明人： Howard M. Fillit ,  Peter C. Harpel

IPC分类号： G01N33/86 ,  G01N33/53

CPC分类号： G01N33/86 ,  G01N2333/8128 ,  G01N2400/40 ,  Y10S436/821

摘要： This invention pertains to a method of detecting, in a sample obtained from an individual, anti-heparin antibodies which inhibit the formation of the heparin accelerated antithrombin III-thrombin complex. In the present method, the presence of such anti-heparin antibodies are detected directly (by detecting the presence of anti-heparin antibodies themselves) or indirectly (by detecting the presence or formation of the heparin accelerated antithrombin III-thrombin complex). In one embodiment of the present method, antibodies which react with or interfere with the heparin pentasaccharide which binds antithrombin III in such a manner that binding to antithrombin III is inhibited are detected. In a specific embodiment of the present method, the anti-heparin antibody detected is one which reacts with or interferes with the disaccharide UA-2S/GlcNs-6 present in residues IV and V of the heparin pentasaccharide that binds antithrombin III.

摘要翻译： 本发明涉及从个体获得的样品中检测抑制肝素加速抗凝血酶III-凝血酶复合物形成的抗肝素抗体的方法。 在本方法中，直接检测这种抗肝素抗体的存在（通过检测本身存在抗肝素抗体）或间接检测（通过检测肝素加速的抗凝血酶III-凝血酶复合物的存在或形成）。 在本方法的一个实施方案中，检测了以与抗凝血酶III结合的方式结合抗凝血酶III的肝素五糖起反应或干扰的抗体。 在本方法的具体实施方案中，检测的抗肝素抗体是与存在于结合抗凝血酶III的肝素五糖的残基IV和V中的二糖UA-2S / GlcNs-6反应或干扰的抗肝素抗体。

公开(公告)号：US5320945A

公开(公告)日：1994-06-14

申请号：US122133

申请日：1993-09-15

申请人： Andreas Dessauer ,  Reinhard Herz ,  Helmut Lill

发明人： Andreas Dessauer ,  Reinhard Herz ,  Helmut Lill

IPC分类号： C12Q1/56

CPC分类号： C12Q1/56 ,  C12Q2337/12 ,  G01N2333/8128 ,  G01N2333/974

摘要： In order to determine antithrombin III in body fluids by reacting the sample with thrombin and a chromogenic substrate which forms a colour by the action of thrombin and measurement of the colour formed, the reaction is carried out in the presence of denaturing agents or of the tetrapeptide Gly-Pro-Arg-Pro and an oligopeptide is used as the substrate which has a sensitivity to thrombin which is a factor of 5 to 100-fold lower compared With Tos-Gly-Pro-Arg-pNA

摘要翻译： 为了通过使样品与凝血酶和形成色素的显色底物通过凝血酶的作用和形成的颜色的测量反应来确定体液中的抗凝血酶III，反应在变性剂或四肽的存在下进行 与Tos-Gly-Pro-Arg-pNA相比，Gly-Pro-Arg-Pro和寡肽用作对凝血酶敏感性为5至100倍的因子的底物

公开(公告)号：US4694070A

公开(公告)日：1987-09-15

申请号：US737761

申请日：1985-05-28

申请人： Gary A. Mitchell ,  Gerald E. Jaffe ,  Marilyn M. Solorzano

发明人： Gary A. Mitchell ,  Gerald E. Jaffe ,  Marilyn M. Solorzano

IPC分类号： C07K1/02 ,  C07K5/08 ,  C07K5/083 ,  C07K5/097 ,  C12Q1/37 ,  C12Q1/56 ,  C07K7/06 ,  C12Q1/38

CPC分类号： C07K5/0821 ,  C07K5/0806 ,  C12Q1/37 ,  C12Q1/56 ,  C12Q2337/40 ,  G01N2333/8128 ,  G01N2333/974 ,  G01N2400/40 ,  Y10S530/802 ,  Y10S930/28

摘要： Water soluble xanthylium derivative substrates of rhodamine 110 and rhodol permit spectrophotometric and fluorescent measurements of trypsin-like enzymes without the addition of organic solvent additives and/or special water solubilizing agents. These novel substrates exhibit increased sensitivity for determining low levels of activity of trypsin-like enzymes such as proteolytic enzymes, cofactors, activators, antiactivators, and inhibitors. These substrates can be substituted for fibrinogen or monitor the pathways of blood coagulation.

摘要翻译： 罗丹明110和rhodol的水溶性xanthylium衍生物底物允许分光光度和荧光测量胰蛋白酶样酶，而不添加有机溶剂添加剂和/或特殊的水增溶剂。 这些新型底物对于确定胰蛋白酶样酶如蛋白水解酶，辅因子，激活剂，抗激动剂和抑制剂的低水平活性具有增加的灵敏度。 这些底物可以代替纤维蛋白原或监测凝血途径。

公开(公告)号：US4543335A

公开(公告)日：1985-09-24

申请号：US450984

申请日：1982-12-20

申请人： Ronald G. Sommer ,  Alfred C. Greenquist

发明人： Ronald G. Sommer ,  Alfred C. Greenquist

IPC分类号： G01N33/66 ,  C12Q1/56 ,  G01N33/48 ,  G01N33/86

CPC分类号： C12Q1/56 ,  G01N2333/8128 ,  G01N2333/974 ,  G01N2400/40

摘要： A device for the quantitative determination of heparin in mammalian blood plasma, a method for the preparation of such device and a method for determining the plasma's heparin concentration using the device. The device, which is prepared using a freeze-drying technique, comprises a first upper layer of a carrier matrix material containing a buffer, AT-III and thrombin and a second, lower layer of a carrier matrix containing a thrombin sensitive fluorogenic or chromogenic substrate being capable of interacting with thrombin in such a manner that a time-related chemical change detectable by fluorometric or spectrophotometric means takes place when thrombin and the substrate are contacted in a suitable liquid environment.

摘要翻译： 用于哺乳动物血浆中肝素定量测定的装置，该装置的制备方法以及使用该装置测定血浆肝素浓度的方法。 使用冷冻干燥技术制备的装置包括含有缓冲液AT-III和凝血酶的载体基质材料的第一上层和含有凝血酶敏感性荧光或显色底物的载体基质的第二下层 能够以这样的方式与凝血酶相互作用，使得当凝血酶和底物在合适的液体环境中接触时，可以通过荧光或分光光度法检测出与时间有关的化学变化。