公开(公告)号：US20190300851A1

公开(公告)日：2019-10-03

申请号：US16302615

申请日：2017-05-16

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Christine CHEUNG ,  Balakrishnan Chakrapani NARMADA ,  Yeek Teck GOH

IPC: C12N5/071 ,  G01N33/50

Abstract: The present disclosure provides a method of deriving endothelial cells, comprising (a) culturing lateral plate mesoderm cells under oxygen-deprived condition to obtain endothelial lineage cells; and (b) culturing endothelial cells from (a) on an extracellular matrix to maintain and expand the endothelial lineage cells. Also disclosed herein is a cell co-culture system comprising an endothelial cell culture and a hepatocyte cell culture, as well as a microfluidic-based system comprising said cell co-culture system. Also disclosed herein is a method of disease modelling or drug testing using said cell co-culture system or said microfluidic-based system.

公开(公告)号：US20190292261A1

公开(公告)日：2019-09-26

申请号：US16360445

申请日：2019-03-21

Applicant: Agency for Science, Technology and Research

Inventor： Cheng-I Wang ,  Hsueh Ling Janice Oh ,  Siok Ping Yeo ,  Jianrong Lionel Low ,  Hwee Ching Tan

IPC: C07K16/28 ,  C07K16/00

Abstract: Anti-PD-1 antibodies are disclosed. Also disclosed are pharmaceutical compositions comprising such antibodies, and uses and methods using the same.

公开(公告)号：US20190285542A1

公开(公告)日：2019-09-19

申请号：US16319155

申请日：2017-07-18

Applicant: Agency for Science, Technology and Research

Inventor： Chi Lok Wong ,  Malini Olivo

IPC: G01N21/552 ,  G01N21/21

Abstract: Various embodiments may provide an optical sensing device based on surface plasmon resonance (SPR). The optical sensing device may include an optical arrangement configured to provide a first polarization light beam and a second polarization light beam, and a first optical member including a sensing surface, the first optical member configured to receive the first and second polarization light beams and reflect the first and second polarization light beams at the sensing surface. The optical sensing device may further include a second optical member arranged to receive the reflected first and second polarization light beams from the first optical member and configured to separate the reflected first and second polarization light beams in a first direction and a second direction, respectively. The optical device may additionally include a detector arrangement configured to detect the reflected first and second polarization light beams from the second optical member.

公开(公告)号：US10414794B2

公开(公告)日：2019-09-17

申请号：US15120111

申请日：2015-02-17

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Peter Stanley Gagnon

IPC: C07K1/18 ,  C07K1/34 ,  C07K16/06 ,  C07K1/36 ,  C07K1/22 ,  C07K1/30 ,  C07K16/00 ,  C07K16/32

Abstract: Provided herein is a novel method of purifying an IgG antibody from a preparation by use of an electropositive membrane having a defined porosity.

公开(公告)号：US10398315B2

公开(公告)日：2019-09-03

申请号：US15038029

申请日：2014-12-08

Applicant: Agency for Science, Technology and Research

Inventor： Malini Olivo ,  Jun Hui Ho ,  Dinish Unnimadhava Kurup Soudamini Amma ,  Chi Lok Wong ,  Ghayathri Balasundaram

IPC: A61B5/00 ,  A61B8/08 ,  A61K49/00 ,  A61K49/22 ,  G01N33/483

Abstract: A method of imaging living tissue is provided. The method includes introducing a photoacoustic contrast agent comprising or consisting of a photosensitizer into living tissue; and obtaining an image of the living tissue by photoacoustic imaging. Use of a photoacoustic contrast agent comprising or consisting of a photosensitizer in photoacoustic imaging is also provided.

公开(公告)号：US20190248879A1

公开(公告)日：2019-08-15

申请号：US16342763

申请日：2017-10-17

Applicant: Agency for Science, Technology and Research ,  Singapore Health Services Pte. Ltd. ,  National University of Singapore

Inventor： Tr Kanaga Sabapathy ,  David P. Lane ,  Le-Ann Hwang ,  Xin Yu Koh ,  Liew Oi Wah

IPC: C07K16/18 ,  A61P35/00

CPC classification number: C07K16/18 ,  A61K2039/505 ,  A61K2039/575 ,  A61P35/00 ,  C07K2317/24 ,  C07K2317/34

Abstract: The invention relates to methods for producing an antibody which is specific for a mutant p53 polypeptide over wildtype p53 polypeptide, comprising using said mutant p53 polypeptide as an immunogen wherein said polypeptide comprises: (i) an antigen sequence, comprising an amino acid sequence of the mutant p53 polypeptide including the mutation and at least one amino acid immediately adjacent to the mutation, and (ii) a scaffold sequence for providing the antigen sequence in a solvent-accessible configuration, in particular wherein the scaffold sequence is thioredoxin. In the specific embodiments, antibodies against mutant p53 comprising R175H, R248Q or R273H are generated. Also disclosed are the uses of said antibodies for diagnosis, prognosis and stratification of patient groups and further encompasses the use of antibodies for imaging and treatment of cancer.

公开(公告)号：US10351827B2

公开(公告)日：2019-07-16

申请号：US15035739

申请日：2014-11-11

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Daniele Zink ,  Jackie Y. Ying

IPC: C12N5/00 ,  C12N5/02 ,  C12N5/071 ,  A61K35/545 ,  A61K35/22 ,  A61L27/36 ,  A61L27/38 ,  A61L27/54 ,  A61L27/58

Abstract: There is provided a method of differentiating an induced pluripotent stem cell (iPSC) into a renal proximal tubular cell (PTC)-like cell. The method comprises culturing an undifferentiated iPSC in a renal epithelial cell culture medium in the presence of one or more extracellular matrix (ECM) molecules, bone morphogenic protein 2 (BMP2) and bone morphogenic protein 7 (BMP7), for a period of from about 8 to about 10 days, under conditions sufficient to induce differentiation of the iPSC into a PTC-like cell. A cell population of differentiated PTC-like cells is also provided, as well as uses and methods of use of the cell population.

公开(公告)号：US20190214736A1

公开(公告)日：2019-07-11

申请号：US16329949

申请日：2017-09-05

Applicant: Agency for Science, Technology and Research

Inventor： Arseniy Kuznetsov ,  Yuan Hsing Fu

IPC: H01Q15/14 ,  G02B1/00 ,  H01Q21/06 ,  H01Q9/04

Abstract: Various embodiments may relate to an antenna. The antenna may include a ridge reflector arranged along a plane. The ridge reflector may be configured to enhance an emission of at least one electromagnetic wave source providing an electromagnetic wave signal to the antenna and further configured to direct the electromagnetic wave signal in a direction at least substantially perpendicular to the plane. The ridge reflector may define a space along the plane for allowing the electromagnetic wave signal to be directed in the direction at least substantially perpendicular to the plane. The ridge reflector may include at least one of a dielectric material and a semiconductor material.

公开(公告)号：US10344301B2

公开(公告)日：2019-07-09

申请号：US15502783

申请日：2015-08-11

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Farid Ghadessy ,  Jia Wei Siau ,  Peter Droge ,  Harshyaa Makhija ,  Shree Harsh Vijaya Chandra ,  Sabrina Peter

IPC: C12N9/12 ,  C12N9/22 ,  C12N1/15 ,  C12N1/21 ,  C12P19/34 ,  C12N15/90

Abstract: The present invention refers to lambda integrases comprising at least one amino acid mutation at positions 43, 319 and 336 of the lambda integrase as set forth in SEQ ID NO: 1. The invention further refers to nucleic acid molecules comprising the nucleotide sequence encoding the mutant lambda integrase and to host cells containing these nucleic acid molecules. The invention also refers to methods of recombining a nucleic acid of interest into a target nucleic acid in the presence of the mutant lambda integrase and sequence specific recombination kits.

公开(公告)号：US20190207424A1

公开(公告)日：2019-07-04

申请号：US16095678

申请日：2017-04-20

Applicant: Agency For Science, Technology and Research

Inventor： Lei YAO ,  Jia Hao Cheong ,  Yuan GAO

IPC: H02J50/12 ,  H02M3/04 ,  H02M1/00 ,  H02J50/80 ,  A61B5/00

CPC classification number: H02J50/12 ,  A61B5/0031 ,  H02J50/80 ,  H02M1/00 ,  H02M3/04

Abstract: A wirelessly powered implantable medical device, a system for synchronized time-division wireless power transfer, and a method for closed-loop carrier waveform adaption for wireless power control are provided. The system for synchronized time-division wireless power transfer includes a wireless transmitter for generating and transmitting time-division wireless power transfer signals and a wirelessly powered device. The wirelessly powered device includes a wireless receiver for receiving the time-division wireless power transfer signals and a time division switching module. The time division switching module is coupled to the wireless receiver and generates multiple supply voltages synchronized to the time-division wireless power transfer signals for powering different circuitry of the wirelessly powered device.