公开(公告)号：US20140302575A1

公开(公告)日：2014-10-09

申请号：US14071318

申请日：2013-11-04

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P7/24 ,  C12P7/04 ,  C12P7/18 ,  C12P7/20

CPC classification number: C12P7/24 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12N15/52 ,  C12P7/04 ,  C12P7/18 ,  C12P7/20 ,  C12Y101/01244 ,  C12Y105/0102

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol. Also provided herein are methods for using such an organism to produce 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol.

Abstract translation: 本文提供了具有甲醇代谢途径的非天然存在的微生物生物，其可以在甲醇存在下增强还原当量的可用性。 这种还原当量可用于提高微生物生物体如1,2-丙二醇，正丙醇，1,3-丙二醇或甘油产生的有机化合物的产物产率。 本文还提供了使用这种生物体来生产1,2-丙二醇，正丙醇，1,3-丙二醇或甘油的方法。

公开(公告)号：US20140302573A1

公开(公告)日：2014-10-09

申请号：US14308292

申请日：2014-06-18

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Robin E. Osterhout ,  Jun Sun

IPC: C12P7/44

CPC classification number: C12P7/44 ,  C12N9/88 ,  C12Y402/01002

Abstract: The invention provides a non-naturally occurring microbial organism having a muconate pathway having at least one exogenous nucleic acid encoding a muconate pathway enzyme expressed in a sufficient amount to produce muconate. The muconate pathway including an enzyme selected from the group consisting of a beta-ketothiolase, a beta-ketoadipyl-CoA hydrolase, a beta-ketoadipyl-CoA transferase, a beta-ketoadipyl-CoA ligase, a 2-fumarylacetate reductase, a 2-fumarylacetate dehydrogenase, a trans-3-hydroxy-4-hexendioate dehydratase, a 2-fumarylacetate aminotransferase, a 2-fumarylacetate aminating oxidoreductase, a trans-3-amino-4-hexenoate deaminase, a beta-ketoadipate enol-lactone hydrolase, a muconolactone isomerase, a muconate cycloisomerase, a beta-ketoadipyl-CoA dehydrogenase, a 3-hydroxyadipyl-CoA dehydratase, a 2,3-dehydroadipyl-CoA transferase, a 2,3-dehydroadipyl-CoA hydrolase, a 2,3-dehydroadipyl-CoA ligase, a muconate reductase, a 2-maleylacetate reductase, a 2-maleylacetate dehydrogenase, a cis-3-hydroxy-4-hexendioate dehydratase, a 2-maleylacetate aminoatransferase, a 2-maleylacetate aminating oxidoreductase, a cis-3-amino-4-hexendioate deaminase, and a muconate cis/trans isomerase. Other muconate pathway enzymes also are provided. Additionally provided are methods of producing muconate.

Abstract translation: 本发明提供了一种非天然存在的微生物，其具有一种具有至少一种外源核酸的粘蛋白途径的微生物生物，所述外源核酸编码以足够量表达的粘蛋白途径酶以产生粘蛋白。 所述粘蛋白途径包括选自β-酮硫解酶，β-酮己二酰辅酶A水解酶，β-酮己二酰辅酶A转移酶，β-酮己二酰辅酶A连接酶，2-富马酰乙酸酯还原酶，2- 富马酰乙酸脱氢酶，反式-3-羟基-4-己二酸脱水酶，2-富马酸乙酯氨基转移酶，2-富马酸乙酯胺化氧化还原酶，反式-3-氨基-4-己烯酸脱氨酶，β-酮己二酸烯醇内酯水解酶， 粘蛋白内酯异构酶，粘蛋白环异构酶，β-酮己二酰辅酶A脱氢酶，3-羟基己二酰辅酶A脱水酶，2,3-脱氢阿迪普基-CoA转移酶，2,3-脱氢阿迪普基-CoA水解酶，2,3-脱氢阿迪普酯 - CoA连接酶，粘蛋白还原酶，2-马来酰乙酸酯还原酶，2-马来酰乙酸酯脱氢酶，顺式-3-羟基-4-己二酸脱水酶，2-马来酰乙酸氨基转移酶，2-马来酰乙酸胺胺化氧化还原酶，顺式-3-氨基 -4-十六酸脱氨酶和粘蛋白顺式/反式异构酶。 还提供了其它粘蛋白途径酶。 另外提供了生产粘蛋白的方法。

公开(公告)号：US20140147900A1

公开(公告)日：2014-05-29

申请号：US13920927

申请日：2013-06-18

Applicant: Genomatica, Inc.

Inventor： John D. Trawick ,  Mark J. Burk ,  Anthony P. Burgard

IPC: C12P7/18

CPC classification number: C12P7/18 ,  C12N9/0006 ,  C12N9/10 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/52 ,  C12P7/04 ,  C12P7/42

Abstract: A non-naturally occurring microbial organism having an isopropanol, 4-hydroxybutryate, or 1,4-butanediol pathway includes at least one exogenous nucleic acid encoding an isopropanol, 4-hydroxybutryate, or 1,4-butanediol pathway enzyme expressed in a sufficient amount to produce isopropanol, 4-hydroxybutryate, or 1,4-butanediol. The aforementioned organisms are cultured to produce isopropanol, 4-hydroxybutryate, or 1,4-butanediol.

Abstract translation: 具有异丙醇，4-羟基丁酸酯或1,4-丁二醇途径的非天然存在的微生物包括至少一种编码以足够量表达的异丙醇，4-羟基丁酸酯或1,4-丁二醇途径酶的外源核酸 产生异丙醇，4-羟基丁酸酯或1,4-丁二醇。 培养上述生物体以产生异丙醇，4-羟基丁酸酯或1,4-丁二醇。

公开(公告)号：US20140011249A1

公开(公告)日：2014-01-09

申请号：US13889056

申请日：2013-05-07

Applicant: Genomatica, Inc.

Inventor： Anthony P. BURGARD ,  Robin E. Osterhout ,  Jun Sun ,  Priti Pharkya

IPC: C12P7/42 ,  C12P7/04 ,  C12P7/18

CPC classification number: C12P7/42 ,  C12N9/0008 ,  C12N9/0095 ,  C12P7/04 ,  C12P7/16 ,  C12P7/18 ,  C12P7/52 ,  Y02E50/10

Abstract: A non-naturally occurring microbial organism includes a microbial organism having a reductive TCA or Wood-Ljungdahl pathway in which at least one exogenous nucleic acid encoding these pathway enzymes is expressed in a sufficient amount to enhance carbon flux through acetyl-CoA. A method for enhancing carbon flux through acetyl-CoA includes culturing theses non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce a product having acetyl-CoA as a building block. Another non-naturally occurring microbial organism includes at least one exogenous nucleic acid encoding an enzyme expressed in a sufficient amount to enhance the availability of reducing equivalents in the presence of carbon monoxide or hydrogen, thereby increasing the yield of redox-limited products via carbohydrate-based carbon feedstock. A method for enhancing the availability of reducing equivalents in the presence of carbon monoxide or hydrogen includes culturing this organism for a sufficient period of time to produce a product.

Abstract translation: 非天然存在的微生物体包括具有还原性TCA或Wood-Ljungdahl途径的微生物生物体，其中至少一种编码这些途径酶的外源核酸以足够的量表达以增强通过乙酰辅酶A的碳通量。 通过乙酰辅酶A增强碳通量的方法包括在条件和足够的时间段内培养这些非天然存在的微生物，以产生具有乙酰辅酶A作为结构单元的产物。 另一种非天然存在的微生物生物体包括至少一种编码以足够量表达的酶的外源核酸，以增强在一氧化碳或氢气存在下还原当量的可用性，从而通过碳水化合物 - 碳原料。 在一氧化碳或氢气存在下增强还原当量的可用性的方法包括在足够的时间内培养该生物体以产生产物。

公开(公告)号：US20130303723A1

公开(公告)日：2013-11-14

申请号：US13730612

申请日：2012-12-28

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Anthony P. Burgard ,  Robin E. Osterhout ,  Priti Pharkya

IPC: C12P17/12 ,  C12P13/00

CPC classification number: C12P17/10 ,  C12N1/38 ,  C12N15/52 ,  C12P7/40 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12P13/02 ,  C12P17/12

Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.

Abstract translation: 本发明提供了具有6-氨基己酸，己内酰胺，六亚甲基二胺或乙酰丙酸途径的非天然存在的微生物生物。 微生物生物体含有至少一种编码各自的6-氨基己酸，己内酰胺，六亚甲基二胺或乙酰丙酸途径中的酶的外源核酸。 本发明另外提供了制备6-氨基己酸，己内酰胺，己二胺或乙酰丙酸的方法。 该方法可以包括培养6-氨基己酸，己内酰胺或六亚甲基二胺生产微生物，其中微生物生物体表达至少一种编码6-氨基己酸，己内酰胺，六亚甲基二胺或乙酰丙酸途径酶的外源核酸，其量足以产生 各自的产物在条件下和足够的时间内产生6-氨基己酸，己内酰胺，六亚甲基二胺或乙酰丙酸。

公开(公告)号：US20250101392A1

公开(公告)日：2025-03-27

申请号：US18662446

申请日：2024-05-13

Applicant: Genomatica, Inc.

Inventor： Andrew M. SHUMAKER ,  Bernardo M.T. DA COSTA ,  Kevin HOLDEN ,  Louis G. HOM ,  Tarah S. BARON ,  Noah HELMAN

IPC: C12N9/18 ,  C12N9/10 ,  C12P7/6436

Abstract: The disclosure relates to enzyme variants with improved ester synthase properties for the production of fatty acid esters. Further contemplated are recombinant host cells that express such variants, cell cultures comprising the recombinant host cells and fatty acid ester compositions produced by such recombinant host cells.

公开(公告)号：US12234463B2

公开(公告)日：2025-02-25

申请号：US17255352

申请日：2019-06-26

Applicant: Genomatica, Inc.

Inventor： Harish Nagarajan ,  Tae Hoon Yang ,  Ali Khodayari

IPC: C12N9/16 ,  C12N9/02 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12P5/00 ,  C12P5/02 ,  C12P7/16 ,  C12P7/18 ,  C12P7/24 ,  C12P7/42 ,  C12P7/46 ,  C12P7/6409 ,  C12P13/00

Abstract: Described herein are engineered cells including ones having synthetic methylotrophy which include an NADH-dependent enzyme capable of converting G3P to 3PG (e.g., B. methanolicus gapN) and/or fructose-1,6-bisphosphatase, along with hexulose-6-phosphate synthase, 6-phospho-3-hexuloisomerase, a phosphoketolase, or a combination thereof. Engineered cells of the disclosure beneficially maintain adequate pool sizes of phosphorylated C3 and/or C4 compounds, and/or provide increased levels of NADPH. As such, the modifications allow for the generation of C6 compounds from C1 (e.g., a methanol feedstod) and C5 compounds, the regeneration of C5 compounds from C6 compounds by carbon rearrangement, and an improved balance between regeneration of C5 compounds and lower glycolysis. In turn, this allows the engineered microorganism to generate sufficient quantities of metabolic precursors (e.g., acetyl-CoA) which can be used in a bioproduct pathway, and the engineered cells can include further modifications to those pathway enzymes allowing for production of a desired bioproduct.

公开(公告)号：US12043859B2

公开(公告)日：2024-07-23

申请号：US17426973

申请日：2020-01-30

Applicant: Genomatica, Inc.

Inventor： Lauri H. Suominen ,  Jefferson Lievense ,  Aiguo Liu ,  Jorge Jesus Martinez-Gonzalez ,  Myong Kon Ko ,  Wei Nan

IPC: C12P7/22 ,  B01D11/02 ,  C12M1/26

CPC classification number: C12P7/22 ,  B01D11/0288 ,  B01D11/0292 ,  C12M33/10

Abstract: Methods of recovering cannabinoids from cell cultures include methods comprising steps of separating the cell culture at a temperature above the melting point of the cannabinoid to separate a light phase comprising liquid state cannabinoid from a heavy phase; and methods comprising treating the cell culture at a temperature below the melting point of the cannabinoid to separate a light phase from a heavy phase comprising solid state cannabinoid. Other methods include contacting the culture with a water-miscible solvent to form a water-miscible phase and an aqueous phase, separating the two phases and recovering the cannabinoid. Other methods include contacting the culture with a water-immiscible solvent to form a water-immiscible phase and an aqueous phase, separating the two phases, and recovering the cannabinoid. Other methods include washing the inner surface of a fermentation vessel with alkaline solution to recover cannabinoid attached to the vessel surface. Various methods make use of aqueous solvent systems comprising no organic solvent, aqueous solvent systems comprising added water-miscible organic solvent, and dual-phase aqueous/water-immiscible solvent systems.

公开(公告)号：US20240225971A1

公开(公告)日：2024-07-11

申请号：US18569956

申请日：2022-06-10

Applicant: Genomatica, Inc.

Inventor： Kyle HUSTON

IPC: A61K8/34 ,  A61Q13/00

CPC classification number: A61K8/345 ,  A61Q13/00

Abstract: The disclosure provides embodiments directed to a composition comprising a 1,3-butylene glycol (1,3-BG; such as but not limited to, bioBG) and one or more fragrance ingredients, which form a fragrance component of the composition. 1,3-BG demonstrates exemplary qualities and characteristics of being natural, sustainable, and substantially non-odorous, for use in fragrance compositions. Methods of using or applying such 1,3-BG fragrance compositions provide a cost-efficient, sustainable composition, since 1,3-BG does not mask or alter the fragrance notes of the fragrance ingredients allowing for the use of smaller quantities of fragrance ingredients. The 1,3-BG fragrance compositions of the disclosure also improve fragrance performance and reduces greenhouse gases, while avoiding any irritation, sensitization, or allergic reaction in humans.

公开(公告)号：US20240190798A1

公开(公告)日：2024-06-13

申请号：US18469170

申请日：2023-09-18

Applicant: Genomatica, Inc.

Inventor： Julia Khandurina ,  Rachel Diane Pacheco ,  Lauri Hannunpoika Suominen ,  Rainer Wagester

IPC: C07C29/80 ,  B01D3/14 ,  B01D15/36 ,  C07C29/94 ,  C07C31/20

CPC classification number: C07C29/80 ,  B01D3/143 ,  B01D15/361 ,  C07C29/94 ,  B01D2202/20 ,  C07C31/207

Abstract: Provided herein are bioderived 1,3-butanediol compositions and systems and processes for producing such bioderived 1,3-butanediol compositions.