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公开(公告)号:US20090156529A1
公开(公告)日:2009-06-18
申请号:US12140112
申请日:2008-06-16
申请人: Gino VAN HEEKE , Emma HICKMAN , Henry Luke DANAHAY , Pamela TAN , Anke GEICK , Hans-Peter VORNLOCHER
发明人: Gino VAN HEEKE , Emma HICKMAN , Henry Luke DANAHAY , Pamela TAN , Anke GEICK , Hans-Peter VORNLOCHER
IPC分类号: A61K31/7105 , C07H21/02 , A61P9/00
CPC分类号: C12N15/1138 , A61K31/713 , A61K45/06 , C12N2310/14 , C12N2310/315 , C12N2310/321 , C12N2310/322 , C12N2310/351 , C12N2310/3513 , C12N2310/3515 , C12N2310/3521
摘要: The invention relates to compositions and methods for modulating the expression of alpha-ENaC, and more particularly to the downregulation of alpha-ENaC expression by chemically modified oligonucleotides.
摘要翻译: 本发明涉及用于调节α-ENaC表达的组合物和方法,更具体地涉及通过化学修饰的寡核苷酸下调α-ENaC表达。
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公开(公告)号:US6127150A
公开(公告)日:2000-10-03
申请号:US927128
申请日:1997-09-05
申请人: Thomas R. Coolidge , Fred Wagner , Gino van Heeke , Sheldon M. Schuster , Jay Stout , Dwane E. Wylie
发明人: Thomas R. Coolidge , Fred Wagner , Gino van Heeke , Sheldon M. Schuster , Jay Stout , Dwane E. Wylie
IPC分类号: C07K14/575 , C07K1/22 , C07K7/14 , C07K14/00 , C07K14/245 , C07K14/47 , C07K14/585 , C07K14/595 , C07K19/00 , C12N1/21 , C12N9/14 , C12N9/88 , C12N15/09 , C12N15/62 , C12P21/00 , C12R1/19 , C12P21/06 , A61K38/24 , C07K1/00 , C12P21/04
CPC分类号: C12N9/14 , C07K14/245 , C07K14/47 , C07K14/585 , C07K14/595 , C07K7/14 , C12N15/62 , C12N9/88 , C07K2319/00 , C07K2319/50 , C07K2319/75
摘要: The present invention is directed to a protein purification construct having three tandem, coupled segments composed of a binding protein, an interconnecting linker and a variable fused polypeptide which incorporates the one or more copies of a product peptide. The binding protein is a mammalian or human carbonic anhydrase, or a modified version of the carbonic anhydrase. The protein purification construct may be employed in methods for expression of the product peptide in microbial and higher organism and for ligand immobilized affinity purification of the product peptide.
摘要翻译: 本发明涉及具有由结合蛋白,互连接头和可变融合多肽组成的三个串联耦合区段的蛋白质纯化构建体,其结合产物肽的一个或多个拷贝。 结合蛋白是哺乳动物或人类碳酸酐酶,或碳酸酐酶的修饰形式。 蛋白质纯化构建体可以用于在微生物和更高级生物体中表达产物肽的方法和用于产物肽的固定化亲和纯化的配体。
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公开(公告)号:US5464759A
公开(公告)日:1995-11-07
申请号:US18100
申请日:1993-02-17
申请人: Thomas R. Coolidge , William Lewis , Sheldon M. Schuster , Jay Stout , Gino van Heeke , Dwane Wylie , Fred W. Wagner
发明人: Thomas R. Coolidge , William Lewis , Sheldon M. Schuster , Jay Stout , Gino van Heeke , Dwane Wylie , Fred W. Wagner
IPC分类号: A61K39/395 , A61K48/00 , C07H21/04 , C07K1/00 , C07K1/02 , C07K1/04 , C07K1/06 , C07K1/113 , C07K1/22 , C07K14/42 , C07K14/575 , C07K14/655 , C07K16/00 , C12N15/20 , C12P19/34 , C12Q1/68
CPC分类号: C07K1/006 , C07K1/04 , C07K1/22 , Y10S435/803 , Y10S435/81 , Y10S436/824
摘要: The invention is directed to a method of purifying sequentially synthesized peptides and oligonucleotides by affinity techniques. Selected products are capped with and N-terminus capping agent for peptides or a 5'-terminus capping agents for oligonucleotides, and then bound with affinity agents that are selective for the corresponding capping agents.
摘要翻译: 本发明涉及通过亲和技术纯化顺序合成的肽和寡核苷酸的方法。 所选择的产物与肽封端的N-末端封端剂或寡核苷酸的5'-末端封端剂,然后与对相应的封端剂有选择性的亲和试剂结合。
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