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公开(公告)号：US20100136660A1
公开(公告)日：2010-06-03
申请号：US12648269
申请日：2009-12-28
Applicant: John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
Inventor： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
IPC: C12N7/01
CPC classification number: C40B40/02 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/55 , C07K2317/622 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , G01N33/53 , G01N33/6857
Abstract: A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.
Abstract translation: 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以分泌重组遗传显示包（rgdp）的表面以功能形式显示 ），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与补充sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合伴侣多肽结合。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而获得在sbp成员中比通过常规方法容易地获得的更大的遗传多样性。
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公开(公告)号：US07662557B2
公开(公告)日：2010-02-16
申请号：US10803653
申请日：2004-03-18
Applicant: John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
Inventor： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
CPC classification number: C40B40/02 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/55 , C07K2317/622 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , G01N33/53 , G01N33/6857
Abstract: A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA.
Abstract translation: 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以分泌重组遗传显示包（rgdp）的表面以功能形式显示 ），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与补充sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。
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公开(公告)号：US07635666B1
公开(公告)日：2009-12-22
申请号：US09416902
申请日：1999-10-13
Applicant: John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Hendricus Reneus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
Inventor： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Hendricus Reneus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
CPC classification number: C07K16/005 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/26 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/34 , C07K2317/55 , C07K2317/62 , C07K2317/622 , C07K2317/92 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , C40B40/02 , G01N33/53 , G01N33/6857 , Y10S530/867
Abstract: A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.
Abstract translation: 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以分泌重组遗传显示包（rgdp）的表面以功能形式显示 ），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与补充sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合伴侣多肽结合。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而获得在sbp成员中比通过常规方法容易地获得的更大的遗传多样性。
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Patent Agency Ranking
公开(公告)号：US20070148774A1
公开(公告)日：2007-06-28
申请号：US11555519
申请日：2006-11-01
Applicant: John McCafferty , Anthony Pope , Kevin Johnson , Hendricus Mattheus Hoogenboom , Andrew Griffiths , Ronald Jackson , Kasper Holliger , James Marks , Timothy Clackson , David Chiswell , Gregory Winter , Timothy Bonnert
Inventor： John McCafferty , Anthony Pope , Kevin Johnson , Hendricus Mattheus Hoogenboom , Andrew Griffiths , Ronald Jackson , Kasper Holliger , James Marks , Timothy Clackson , David Chiswell , Gregory Winter , Timothy Bonnert
CPC classification number: C07K16/005 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/26 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/34 , C07K2317/55 , C07K2317/62 , C07K2317/622 , C07K2317/92 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , C40B40/02 , G01N33/53 , G01N33/6857 , Y10S530/867
Abstract: A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.
Abstract translation: 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以分泌重组遗传显示包（rgdp）的表面以功能形式显示 ），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与补充sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合伴侣多肽结合。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而获得在sbp成员中比通过常规方法容易地获得的更大的遗传多样性。
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公开(公告)号：US09150659B2
公开(公告)日：2015-10-06
申请号：US13981697
申请日：2012-01-30
Applicant: Gillian Murphy , Christopher Tape , John McCafferty
Inventor： Gillian Murphy , Christopher Tape , John McCafferty
CPC classification number: C07K16/40 , A61K2039/505 , C07K16/00 , C07K16/2896 , C07K2317/30 , C07K2317/34 , C07K2317/56 , C07K2317/76 , C07K2317/92
Abstract: Anti-TACE (ADAM17) antibodies are disclosed that for the first time in the art are capable of binding to TACE, of acting as antagonists of one or more its biological activities, in particular by binding to TACE in a cross domain binding mode in which residues in both the catalytic domain and in the cysteine rich/disintegrin domains (Dis-Cys) are involved in antibody binding to TACE, helping to improve the specificity of the antibody binding and/or helping to improve inhibition of TACE biological activity. The therapeutic uses of the antibodies, in particular for the treatment of cancer are disclosed.
Abstract translation: 公开了抗TACE（ADAM17）抗体，本领域首次能够结合TACE，作为一种或多种其生物学活性的拮抗剂，特别是通过以交叉结构结合模式结合TACE，其中 催化结构域和半胱氨酸富集/去整合素结构域（Dis-Cys）中的残基都参与与TACE的抗体结合，有助于改善抗体结合的特异性和/或有助于改善TACE生物活性的抑制。 公开了抗体的治疗用途，特别是用于治疗癌症。
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公开(公告)号：US07732377B2
公开(公告)日：2010-06-08
申请号：US10803622
申请日：2004-03-18
Applicant: John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
Inventor： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
CPC classification number: C40B40/02 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/55 , C07K2317/622 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , G01N33/53 , G01N33/6857
Abstract: A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a filamentous bacteriophage particle containing DNA encoding the sbp member, wherein the sbp member has a binding domain that consists of a dAb fragment. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected filamentous bacteriophage particles for expression of the selected sbp members.
Abstract translation: 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这样的sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以功能形式显示在含有编码DNA的DNA的丝状噬菌体颗粒的表面 sbp成员，其中sbp成员具有由dAb片段组成的结合结构域。 可以通过与互补sbp成员的亲和力来选择显示的sbps，并且从所选择的丝状噬菌体颗粒中回收DNA以表达所选择的sbp成员。
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公开(公告)号：US06806079B1
公开(公告)日：2004-10-19
申请号：US09726219
申请日：2000-11-28
Applicant: John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
Inventor： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
IPC: C12N1500
CPC classification number: C40B40/02 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/55 , C07K2317/622 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , G01N33/53 , G01N33/6857
Abstract: A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.
Abstract translation: 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以分泌重组遗传显示包（rgdp）的表面以功能形式显示 ），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与补充sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合伴侣多肽结合。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而获得在sbp成员中比通过常规方法容易地获得的更大的遗传多样性。
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28.发明授权公开(公告)号：US06593081B1
公开(公告)日：2003-07-15
申请号：US09532840
申请日：2000-03-21
Applicant: Andrew David Griffiths , Hendricus Renerus Jacobus Mattheus Hoogenboom , James David Marks , John McCafferty , Gregory Paul Winter , Geoffrey Walter Grigg
Inventor： Andrew David Griffiths , Hendricus Renerus Jacobus Mattheus Hoogenboom , James David Marks , John McCafferty , Gregory Paul Winter , Geoffrey Walter Grigg
IPC: C12O170
CPC classification number: C40B40/02 , B01J2219/00718 , C07K16/005 , C07K16/18 , C07K16/22 , C07K16/241 , C07K16/26 , C07K16/28 , C07K16/2812 , C07K16/2863 , C07K16/2866 , C07K16/3007 , C07K16/3092 , C07K16/34 , C07K16/40 , C07K16/4208 , C07K16/4241 , C07K16/44 , C07K16/462 , C07K16/468 , C07K2317/21 , C07K2317/24 , C07K2317/55 , C07K2317/622 , C07K2317/92 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , Y10S530/867
Abstract: Methods are disclosed for the production of human self-antibodies and antibody fragments, which bind human antigens. Methods comprise providing a library of replicable genetic display packages (rgdps), such as filamentous phage, each rgdp displaying at its surface a member of a specific binding pair which is an antibody or antibody fragment, and each rgdp containing nucleic acid sequence derived from a species of mammal. The nucleic acid sequence in each rgdp encodes a polypeptide chain which is a component part of the sbp member displayed at the surface of that rgdp. Human antibodies or antibody fragments are selected by binding with human antigens. The displayed antibody fragments may be scFv, Fd, Fab or any other fragment which has the capability of binding to and is a human antigen. Nucleic acid libraries used may be derived from V-gene sequences of unimmunised humans. Part or all of the nucleic acid may be derived from oligonucleotide synthesis.
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