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公开(公告)号：US07635666B1
公开(公告)日：2009-12-22
申请号：US09416902
申请日：1999-10-13
申请人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Hendricus Reneus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
发明人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Hendricus Reneus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
CPC分类号： C07K16/005 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/26 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/34 , C07K2317/55 , C07K2317/62 , C07K2317/622 , C07K2317/92 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , C40B40/02 , G01N33/53 , G01N33/6857 , Y10S530/867
摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.
摘要翻译： 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以分泌重组遗传显示包（rgdp）的表面以功能形式显示 ），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与补充sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合伴侣多肽结合。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而获得在sbp成员中比通过常规方法容易地获得的更大的遗传多样性。
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公开(公告)号：US07063943B1
公开(公告)日：2006-06-20
申请号：US09196673
申请日：1998-11-20
申请人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
发明人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
CPC分类号： C40B40/02 , C12N15/1037
摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.
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公开(公告)号：US06916605B1
公开(公告)日：2005-07-12
申请号：US09196522
申请日：1998-11-20
申请人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Hendricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
发明人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Hendricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
IPC分类号： C07K16/00 , C07K16/18 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/46 , C07K19/00 , C12N15/10 , C12N15/62 , C12N15/73 , C40B40/02 , G01N33/53 , G01N33/68 , C12Q1/70 , C07H21/02 , C12N15/00
CPC分类号： C40B40/02 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/55 , C07K2317/622 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , G01N33/53 , G01N33/6857
摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.
摘要翻译： 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以分泌重组遗传显示包（rgdp）的表面以功能形式显示 ），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与补充sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合伴侣多肽结合。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而获得在sbp成员中比通过常规方法容易地获得的更大的遗传多样性。
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公开(公告)号：US07732377B2
公开(公告)日：2010-06-08
申请号：US10803622
申请日：2004-03-18
申请人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
发明人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
CPC分类号： C40B40/02 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/55 , C07K2317/622 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , G01N33/53 , G01N33/6857
摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a filamentous bacteriophage particle containing DNA encoding the sbp member, wherein the sbp member has a binding domain that consists of a dAb fragment. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected filamentous bacteriophage particles for expression of the selected sbp members.
摘要翻译： 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这样的sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以功能形式显示在含有编码DNA的DNA的丝状噬菌体颗粒的表面 sbp成员，其中sbp成员具有由dAb片段组成的结合结构域。 可以通过与互补sbp成员的亲和力来选择显示的sbps，并且从所选择的丝状噬菌体颗粒中回收DNA以表达所选择的sbp成员。
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公开(公告)号：US06806079B1
公开(公告)日：2004-10-19
申请号：US09726219
申请日：2000-11-28
申请人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
发明人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
IPC分类号： C12N1500
CPC分类号： C40B40/02 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/55 , C07K2317/622 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , G01N33/53 , G01N33/6857
摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.
摘要翻译： 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以分泌重组遗传显示包（rgdp）的表面以功能形式显示 ），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与补充sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合伴侣多肽结合。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而获得在sbp成员中比通过常规方法容易地获得的更大的遗传多样性。
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公开(公告)号：US20100136660A1
公开(公告)日：2010-06-03
申请号：US12648269
申请日：2009-12-28
申请人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
发明人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
IPC分类号： C12N7/01
CPC分类号： C40B40/02 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/55 , C07K2317/622 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , G01N33/53 , G01N33/6857
摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.
摘要翻译： 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以分泌重组遗传显示包（rgdp）的表面以功能形式显示 ），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与补充sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合伴侣多肽结合。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而获得在sbp成员中比通过常规方法容易地获得的更大的遗传多样性。
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公开(公告)号：US07662557B2
公开(公告)日：2010-02-16
申请号：US10803653
申请日：2004-03-18
申请人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
发明人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
CPC分类号： C40B40/02 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/55 , C07K2317/622 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , G01N33/53 , G01N33/6857
摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA.
摘要翻译： 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以分泌重组遗传显示包（rgdp）的表面以功能形式显示 ），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与补充sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。
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公开(公告)号：US06172197B2
公开(公告)日：2001-01-09
申请号：US08484893
申请日：1995-06-07
申请人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
发明人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
IPC分类号： C07K1600
CPC分类号： C07K16/468 , C07K16/18 , C07K16/462 , C07K2317/24 , C07K2319/02 , Y10S530/867
摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.
摘要翻译： 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以分泌重组遗传显示包（rgdp）的表面以功能形式显示 ），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 可以通过与互补sbp成员的亲和力来选择显示的sbps，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合配偶体多肽结合。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而在sbp成员中获得比通过常规方法容易获得的更大的遗传多样性。
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公开(公告)号：US5969108A
公开(公告)日：1999-10-19
申请号：US971857
申请日：1993-01-08
申请人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
发明人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
IPC分类号： C12N1/21 , C07H21/00 , C07K16/00 , C07K16/18 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/46 , C07K19/00 , C12N7/00 , C12N7/01 , C12N15/00 , C12N15/09 , C12N15/10 , C12N15/13 , C12N15/62 , C12N15/73 , C12P21/02 , C12R1/19 , C40B40/02 , G01N33/53 , G01N33/531 , G01N33/68 , C07H21/04
CPC分类号： C07K16/005 , C07K16/00 , C07K16/18 , C07K16/26 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C12N15/1037 , C12N15/62 , C12N15/73 , C40B40/02 , G01N33/53 , G01N33/6857 , B01J2219/00718 , C07K2317/21 , C07K2317/24 , C07K2317/34 , C07K2317/55 , C07K2317/62 , C07K2317/622 , C07K2317/92 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , Y10S530/867
摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.
摘要翻译： PCT No.PCT / GB91 / 01134 Sec。 371日期：1993年1月8日 102（e）日期1993年1月8日PCT 1991年7月10日PCT公布。 出版物WO92 / 01047 日期1992年1月23日通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定特异性结合对（sbp）的成员，其中sbp成员以分泌重组体的表面的功能形式显示 遗传显示包（rgdp），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与补充sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合伴侣多肽结合。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而获得在sbp成员中比通过常规方法容易地获得的更大的遗传多样性。
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公开(公告)号：US20120129710A1
公开(公告)日：2012-05-24
申请号：US13165300
申请日：2011-06-21
申请人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Hendricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kasper Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
发明人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Hendricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kasper Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
CPC分类号： C07K16/005 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/26 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/34 , C07K2317/55 , C07K2317/62 , C07K2317/622 , C07K2317/92 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , C40B40/02 , G01N33/53 , G01N33/6857 , Y10S530/867
摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.
摘要翻译： 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以分泌重组遗传显示包（rgdp）的表面以功能形式显示 ），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与补充sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合伴侣多肽结合。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而获得在sbp成员中比通过常规方法容易地获得的更大的遗传多样性。
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