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    Method for the Analysis of Methylated Dna
    21.
    发明申请
    Method for the Analysis of Methylated Dna 审中-公开
    甲基化Dna分析方法

    公开(公告)号：US20070254293A1

    公开(公告)日：2007-11-01

    申请号：US11660804

    申请日：2005-08-09

    申请人： David Guetig ,  Matthias Schuster ,  Juergen Distler

    发明人： David Guetig ,  Matthias Schuster ,  Juergen Distler

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6853 ,  C12Q1/6858 ,  C12Q2525/186 ,  C12Q2523/125 ,  C12Q2565/301 ,  C12Q2535/125

    摘要： The present invention relates to a method for the analysis of methylated cytosines in DNA. In the first step of the invention unmethylated cytosines in the DNA to be analysed are chemically converted into uracil while 5-methylcytosines remain unchanged. In a second step a methylation specific oligonucleotide carrying a non-extendable 3′ end is annealed to the converted DNA. Subsequently, the non-extendable 3′ terminus of the oligonucleotide is removed in case the oligonucleotide is bound to the DNA with the methylation status to be detected. Finally the unblocked oligonucleotide is extended, and the methylation status is concluded from the absence or presence of an extended oligonucleotide product. The method is preferably used for diagnosis and/or prognosis of adverse events for individuals, for distinguishing cell types and tissues, or for investigating cell differentiation.

    摘要翻译： 本发明涉及DNA分析甲基化胞嘧啶的方法。 在本发明的第一步中，待分析DNA中的未甲基化胞嘧啶化学转化成尿嘧啶，而5-甲基胞嘧啶保持不变。 在第二步中，携带不可延伸的3'末端的甲基化特异性寡核苷酸与转化的DNA退火。 随后，在寡核苷酸与具有待检测甲基化状态的DNA结合的情况下，去除寡核苷酸的不可延伸的3'末端。 最后，未阻断的寡核苷酸被延长，甲基化状态从延伸的寡核苷酸产物的不存在或存在而得出。 该方法优选用于个体的不良事件的诊断和/或预后，用于区分细胞类型和组织，或用于调查细胞分化。

    Device for picking up a sheet trailing edge from an upstream cylinder and for transferring the sheet trailing edge to a gripper system
    23.
    发明授权
    Device for picking up a sheet trailing edge from an upstream cylinder and for transferring the sheet trailing edge to a gripper system 失效
    用于从上游圆筒拾取片材后缘并将片材后缘传送到夹具系统的装置

    公开(公告)号：US06752392B2

    公开(公告)日：2004-06-22

    申请号：US10254728

    申请日：2002-09-25

    申请人： Andreas Fricke ,  Michael Puckl ,  Ludwig Reinhard ,  Matthias Schuster ,  Gunter Siebdrath

    发明人： Andreas Fricke ,  Michael Puckl ,  Ludwig Reinhard ,  Matthias Schuster ,  Gunter Siebdrath

    IPC分类号： B65H2900

    CPC分类号： B41F21/106 ,  B65H5/12 ,  B65H5/14 ,  B65H2404/656 ,  B65H2701/1313

    摘要： A device for picking up a sheet trailing edge from an upstream cylinder and transferring the sheet trailing edge to a gripper system of a downstream drum, as viewed in sheet travel direction, includes a sucker guide mechanism having a movably mounted sucker carrier whereon sucker heads subjectible to an application of vacuum or suction air are disposed. The sucker carrier is constructed as a tubular part having a longitudinal axis and being movable perpendicularly to the longitudinal axis thereof. The tubular part is formed with a vacuum or suction air duct extending in the interior thereof and is formed of carbon fiber-reinforced plastic-material laminate.

    摘要翻译： 一种用于从上游圆筒拾取片材后缘并将片材后缘转移到下游鼓的夹持系统的装置，如从片材行进方向看，包括一个吸盘引导机构，其具有可移动地安装的吸盘载体，吸盘可吸入 设置真空或抽吸空气的应用。 吸盘载体构造为具有纵向轴线并且可垂直于其纵向轴线移动的管状部件。 管状部分形成有在其内部延伸的真空或吸入空气管道，并由碳纤维增强的塑料材料层压体形成。

    Process for solid phase glycopeptide synthesis
    24.
    发明授权
    Process for solid phase glycopeptide synthesis 失效
    固相糖肽合成方法

    公开(公告)号：US5369017A

    公开(公告)日：1994-11-29

    申请号：US191777

    申请日：1994-02-04

    申请人： Chi-Huey Wong ,  Matthias Schuster

    发明人： Chi-Huey Wong ,  Matthias Schuster

    IPC分类号： C07K1/04 ,  C12P21/00

    CPC分类号： C07K1/042

    摘要： A process for the synthesis of a glycopeptide using a solid phase matrix is disclosed. The matrix is compatible with aqueous and organic solvents and is comprised of a silica-based solid support to which is linked a two-part spacer group having a chain length of about 12 to about 40 methylene groups. The first part of the spacer is covalently bonded to the silica-based support and has a length of about 3 to about 10 methylene groups. The second spacer part is covalently bonded to the first part of the spacer and comprises the distal end of the two part spacer. The second part is soluble as a free molecule in each of water, dimethylformamide and dichloromethane and has a terminal amine or hydroxyl group to which the C-terminal residue of the peptide portion of the glycopeptide chain is bonded. The chain of atoms connecting the desired glycopeptide to the solid phase matrix also includes a moiety having a selectively severable bond which on cleavage of that bond separates the matrix from whatever else is bonded to that moiety.

    摘要翻译： 公开了使用固相基质合成糖肽的方法。 该基质与水性和有机溶剂相容，并且由二氧化硅基固体载体组成，其连接有链长约12至约40个亚甲基的两部分间隔基团。 间隔基的第一部分共价结合到二氧化硅基载体上，其长度为约3至约10个亚甲基。 第二间隔部分共价结合到间隔物的第一部分并且包括两部分间隔物的远端。 第二部分作为水，二甲基甲酰胺和二氯甲烷中的每一个中的游离分子是可溶的，并且具有结合糖肽链的肽部分的C末端残基的末端胺或羟基。 将所需糖肽连接到固相基质上的原子链还包括具有选择性可分离键的部分，其在该键的切割时将基质与与该部分键合的任何其它基团分开。

    LOAD LIFTING APPARATUS FOR A HELICOPTER AND HELICOPTER
    27.
    发明申请
    LOAD LIFTING APPARATUS FOR A HELICOPTER AND HELICOPTER 有权
    直升机和直升机的装载提升装置

    公开(公告)号：US20120145832A1

    公开(公告)日：2012-06-14

    申请号：US13325606

    申请日：2011-12-14

    申请人： Matthias SCHUSTER

    发明人： Matthias SCHUSTER

    IPC分类号： B64D1/22 ,  B66D3/18

    CPC分类号： B64D1/22 ,  B66D3/18

    摘要： A load lifting apparatus for a helicopter has a cable, including a supply length in a cable store. The cable is secured at one end to the helicopter and has a free end. A load-bearing element, on which a load to be raised can be secured, is arranged on the cable. The cable can be removed from the cable store in order to lower the load-bearing element downwards from the helicopter. As the load-bearing element is lowered or pulled upwards, the cable acts at a force-introduction location on the helicopter. The load-bearing element is arranged on the cable such that it can move along the cable. At least one cable-attachment location is present on the helicopter, and is spaced apart from the force-introduction location and has, or can have, the free end of the cable secured on it.

    摘要翻译： 用于直升机的负载提升装置具有电缆，包括在电缆存储器中的供应长度。 电缆一端固定在直升机上，并具有自由端。 在该电缆上设置承载元件，在该载荷元件上可以固定待升起的载荷。 电缆可以从电缆仓中取出，以便从直升机向下降负载元件。 当承载元件下降或向上拉时，电缆作用在直升机上的力导入位置。 承载元件设置在电缆上，使其能够沿着电缆移动。 在直升机上存在至少一个电缆连接位置，并且与力导入位置间隔开，并且具有或可以具有固定在其上的电缆的自由端。

    Method For Determining The Methylation Rate of a Nucleic Acid
    29.
    发明申请
    Method For Determining The Methylation Rate of a Nucleic Acid 审中-公开
    确定核酸甲基化速率的方法

    公开(公告)号：US20100003680A1

    公开(公告)日：2010-01-07

    申请号：US12309555

    申请日：2006-07-18

    申请人： Joern Lewin ,  Ralf Lesche ,  Matthias Schuster ,  Dimo Dietrich

    发明人： Joern Lewin ,  Ralf Lesche ,  Matthias Schuster ,  Dimo Dietrich

    IPC分类号： C12Q1/68 ,  C07H21/00

    CPC分类号： C12Q1/6869

    摘要： The invention relates to a method for quantitatively determining the methylation rate of a nucleic acid through sequencing. According to the invention, the method comprises at least the following steps: a) treating the nucleic acid with a chemical reagent or an enzyme containing solution, whereby the base pairing behavior of methylated cytosine bases and/or unmethylated cytosine bases of the nucleic acid are altered such that methylated cytosine bases become distinguishable from unmethylated cytosine bases, and b) introducing into the nucleic acid at least one base for generating a sequencing signal to be used as a reference signal for normalization, and c) sequencing the nucleic acid, whereby a signal from each cytosine base of the nucleic acid, or a signal from each guanine base of the nucleic acid and a reference signal from the at least on introduced base is obtained, and d) normalizing the signal obtained from each cytosine base of the nucleic acid, or the signal obtained from each guanine base of the nucleic acid to the reference signal from the at least one introduced base.

    摘要翻译： 本发明涉及通过测序定量测定核酸的甲基化速率的方法。 根据本发明，该方法至少包括以下步骤：a）用化学试剂或含酶溶液处理核酸，由此核酸的甲基化胞嘧啶碱基和/或未甲基化的胞嘧啶碱基的碱基配对行为是 改变为使得甲基化胞嘧啶碱基与非甲基化胞嘧啶碱基区分开，以及b）将至少一个碱基引入核酸，以产生用作标准化参考信号的测序信号，和c）对核酸进行测序，由此 获得来自核酸的每个胞嘧啶碱基的信号或来自核酸的每个鸟嘌呤碱基的信号和来自至少一个引入的碱基的参考信号，以及d）使从核酸的每个胞嘧啶碱基获得的信号归一化 或从核酸的每个鸟嘌呤碱基获得的信号至至少一个引入的碱基的参考信号。

    Method for analysis of cytosine methylation
    30.
    发明申请
    Method for analysis of cytosine methylation 审中-公开
    胞嘧啶甲基化分析方法

    公开(公告)号：US20090075251A1

    公开(公告)日：2009-03-19

    申请号：US10594013

    申请日：2005-03-24

    申请人： Dimo Dietrich ,  Philipp Schatz ,  Matthias Schuster ,  Antje Kluth

    发明人： Dimo Dietrich ,  Philipp Schatz ,  Matthias Schuster ,  Antje Kluth

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6858 ,  C12Q2525/143 ,  C12Q2523/125

    摘要： A method for the analysis of cytosine methylations in DNA is described. Here, the DNA to be investigated is first chemically or enzymatically converted. Then a promoter is introduced into the DNA. After this, the DNA is converted to RNA. The methylation pattern of the DNA can be concluded in different ways by means of an analysis of the RNA. The RNA is preferably fragmented chemically or enzymatically prior to the analysis, whereby the fragmenting results depend on the methylation pattern of the DNA. The method according to the invention is particularly suitable for the diagnosis and prognosis of cancer disorders and other diseases associated with a modification of the methylation pattern.

    摘要翻译： 描述了分析DNA中胞嘧啶甲基化的方法。 这里，待研究的DNA首先被化学或酶转化。 然后将启动子引入DNA。 之后，将DNA转化为RNA。 DNA的甲基化模式可以通过RNA的分析以不同的方式得出结论。 优选在分析之前将RNA片段化学或酶促，由此片段化结果取决于DNA的甲基化模式。 根据本发明的方法特别适合于与甲基化模式的改变相关的癌症病症和其它疾病的诊断和预后。