公开(公告)号：US20060264620A1

公开(公告)日：2006-11-23

申请号：US11345174

申请日：2006-02-01

Applicant: Myo-yong Lee ,  Joong-gun Lee ,  Young-nam Kwon ,  Young-a Kim ,  Yeon-ja Cho ,  Shin-i Yoo

Inventor： Myo-yong Lee ,  Joong-gun Lee ,  Young-nam Kwon ,  Young-a Kim ,  Yeon-ja Cho ,  Shin-i Yoo

IPC: C07H21/04

CPC classification number: C07H21/04 ,  C12N15/1006

Abstract: Provided are methods of isolating and amplifying nucleic acids from and in a nucleic acid-containing sample. The nucleic acid isolation method includes contacting a nucleic acid-containing sample to a silanized solid support to capture nucleic acids to the silanized solid support and treating the nucleic acid-captured solid support with an alkaline solution of pH 9 to 14. The nucleic acid amplification method includes contacting a nucleic acid-containing sample to a silanized solid support to capture nucleic acids to the silanized solid support; treating the nucleic acid-captured solid support with an alkaline solution of pH 9 to 14; and adding a nucleic acid amplification solution to the resultant solution after the alkaline solution treatment to perform nucleic acid amplification.

Abstract translation: 提供从含核酸样品中分离和扩增核酸的方法。 核酸分离方法包括将含核酸的样品与硅烷化的固体支持物接触以将核酸捕获到硅烷化的固体支持物，并用pH9至14的碱性溶液处理核酸捕获的固体支持物。核酸扩增 方法包括将含核酸的样品与硅烷化的固体支持物接触以将核酸捕获到硅烷化固体支持物; 用pH9至14的碱性溶液处理核酸捕获的固体支持物; 并在碱溶液处理后向所得溶液中加入核酸扩增溶液进行核酸扩增。

公开(公告)号：US08518642B2

公开(公告)日：2013-08-27

申请号：US12684345

申请日：2010-01-08

Applicant: Kyung-hee Park ,  Myo-yong Lee ,  Kyu-sang Lee ,  Jong-suk Chung

Inventor： Kyung-hee Park ,  Myo-yong Lee ,  Kyu-sang Lee ,  Jong-suk Chung

IPC: C12Q1/68 ,  C12P19/34 ,  C12M1/34 ,  C12M3/00 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12Q1/6837 ,  C12Q2545/101

Abstract: Provided are a method of analyzing a sequence of a first probe nucleic acid using a substrate on which a second probe nucleic acid is immobilized, and a microarray and a kit for the same.

Abstract translation: 提供了使用其上固定有第二探针核酸的底物和微阵列及其用于分析第一探针核酸的试剂盒的方法来分析第一探针核酸的序列。

公开(公告)号：US07785787B2

公开(公告)日：2010-08-31

申请号：US11345174

申请日：2006-02-01

Applicant: Myo-yong Lee ,  Joong-gun Lee ,  Young-nam Kwon ,  Young-a Kim ,  Yeon-ja Cho ,  Shin-i Yoo

Inventor： Myo-yong Lee ,  Joong-gun Lee ,  Young-nam Kwon ,  Young-a Kim ,  Yeon-ja Cho ,  Shin-i Yoo

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C07H21/04 ,  C12N15/1006

Abstract: Provided are methods of isolating and amplifying nucleic acids from and in a nucleic acid-containing sample. The nucleic acid isolation method includes contacting a nucleic acid-containing sample to a silanized solid support to capture nucleic acids to the silanized solid support and treating the nucleic acid-captured solid support with an alkaline solution of pH 9 to 14. The nucleic acid amplification method includes contacting a nucleic acid-containing sample to a silanized solid support to capture nucleic acids to the silanized solid support; treating the nucleic acid-captured solid support with an alkaline solution of pH 9 to 14; and adding a nucleic acid amplification solution to the resultant solution after the alkaline solution treatment to perform nucleic acid amplification.

Abstract translation: 提供从含核酸样品中分离和扩增核酸的方法。 核酸分离方法包括将含核酸的样品与硅烷化的固体支持物接触以将核酸捕获到硅烷化的固体支持物，并用pH9至14的碱性溶液处理核酸捕获的固体支持物。核酸扩增 方法包括将含核酸的样品与硅烷化的固体支持物接触以将核酸捕获到硅烷化固体支持物; 用pH9至14的碱性溶液处理核酸捕获的固体支持物; 并在碱溶液处理后向所得溶液中加入核酸扩增溶液进行核酸扩增。

公开(公告)号：US20080306249A1

公开(公告)日：2008-12-11

申请号：US12107498

申请日：2008-04-22

Applicant: Myo-yong LEE ,  Ki-woong HAN

Inventor： Myo-yong LEE ,  Ki-woong HAN

IPC: C07K1/00

CPC classification number: C07K1/36 ,  C07K1/20 ,  C12Q1/6806 ,  C12Q2527/125

Abstract: Provided is a method of removing protein while not removing nucleic acids from a biological sample containing protein, the method including: adding a compound of formula I below and a protein nucleating agent to the biological sample containing protein: where at least two of R1, R2, and R3 substituents are substituted or unsubstituted C1-C6 alkyl groups and the other substituent is a hydrogen atom or a substituted or unsubstituted C1-C6 alkyl group, a is an integer of 1 to 6 and b is 0 or 1, wherein b is 0 when a is not 1; treating the resultant mixture with a hydrophobic surface material in order to obtain a protein-free mixture; and separating the protein-free mixture from the hydrophobic surface material to which the protein is bound. By using the method, the protein can be selectively, effectively removed from the biological sample containing the protein while a nucleic acid is maintained in the sample.

Abstract translation: 提供一种除去蛋白质而不从含有生物样品的蛋白质中除去核酸的方法，该方法包括：将下面的式I化合物和蛋白质成核剂加入到含有蛋白质的生物样品中：其中R1，R2中的至少两个 和R3取代基是取代或未取代的C1-C6烷基，另一个取代基是氢原子或取代或未取代的C1-C6烷基，a是1〜6的整数，b是0或1，其中b是 当a不为1时为0; 用疏水性表面材料处理所得混合物以获得无蛋白质的混合物; 并将蛋白质与蛋白质结合的疏水表面材料分离出来。 通过使用该方法，可以在将样品中保持核酸的同时，从含有蛋白质的生物样品中有效地除去蛋白质。

公开(公告)号：US20080070250A1

公开(公告)日：2008-03-20

申请号：US11736033

申请日：2007-04-17

Applicant: Myo-yong LEE

Inventor： Myo-yong LEE

IPC: C12Q1/68 ,  C12M1/34

CPC classification number: B01L3/50857 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/14 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2400/043 ,  C12Q1/6837

Abstract: A method of removing air bubbles from a hybridization solution in a microarray-coverslip assembly c including injecting a solution of magnetic particles between the coverslip and microarray, applying a magnetic field to the microarray-coverslip assembly, moving the magnetic particles and removing air bubbles in the hybridization solution from a hybridization area of the microarray with the magnetic particles, and a microarray kit for the same.

Abstract translation: 一种在微阵列盖玻片组件c中从杂交溶液中除去气泡的方法，包括在盖玻片和微阵列之间注入磁性颗粒溶液，向微阵列 - 盖玻片组件施加磁场，移动磁性颗粒并除去气泡 来自微阵列与磁性颗粒的杂交区域的杂交溶液，以及用于其的微阵列试剂盒。

公开(公告)号：US20060223100A1

公开(公告)日：2006-10-05

申请号：US11396761

申请日：2006-04-03

Applicant: Myo-yong Lee ,  Ki-woong Han

Inventor： Myo-yong Lee ,  Ki-woong Han

IPC: C07H21/04 ,  C12Q1/68

CPC classification number: C07K1/36 ,  C07K1/20 ,  C12Q1/6806 ,  C12Q2527/125

Abstract: Provided is a method of removing protein while not removing nucleic acids from a biological sample containing protein, the method including: adding a compound of formula I below and a protein nucleating agent to the biological sample containing protein: where at least two of R1, R2, and R3 substituents are substituted or unsubstituted C1-C6 alkyl groups and the other substituent is a hydrogen atom, and a is an integer of 1 to 6; treating the resultant mixture with a hydrophobic surface material in order to obtain a protein-free mixture; and separating the protein-free mixture from the hydrophobic surface material to which the protein is bound. By using the method, the protein can be selectively, effectively removed from the biological sample containing the protein while a nucleic acid is maintained in the sample.

Abstract translation: 提供一种除去蛋白质而不从含有生物样品的蛋白质中除去核酸的方法，该方法包括：将下面的式I化合物和蛋白质成核剂加入到含有蛋白质的生物样品中：其中至少两个R R 1，R 2，R 3和R 3取代基是取代或未取代的C 1 -C 6烷基，而另一个取代基是氢原子，a是 1〜6的整数; 用疏水性表面材料处理所得混合物以获得无蛋白质的混合物; 并将蛋白质与蛋白质结合的疏水表面材料分离出来。 通过使用该方法，可以在将样品中保持核酸的同时，从含有蛋白质的生物样品中有效地除去蛋白质。

公开(公告)号：US20060204985A1

公开(公告)日：2006-09-14

申请号：US11345018

申请日：2006-02-01

Applicant: Joon-shik Park ,  Myo-yong Lee

Inventor： Joon-shik Park ,  Myo-yong Lee

IPC: C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12Q1/6837 ,  C12Q2527/125

Abstract: Provided are a method of hybridizing genes, including applying to a gene microarray a hybridization solution in which a compound represented by formula (1) is added: where each of R1, R2, and R3 is independently a straight or branched C1-C5 alkyl; and X−is an anion of an organic acid; and a method of using a compound represented by formula (1) as an additive to a hybridization solution.

Abstract translation: 提供了将基因杂交的方法，包括向基因芯片应用添加了式（1）所示的化合物的杂交溶液：其中R 1，R 2， 而且R 3独立地为直链或支链C 1 -C 5烷基; 且X - 是有机酸的阴离子; 以及使用由式（1）表示的化合物作为杂交溶液的添加剂的方法。

公开(公告)号：US20060084165A1

公开(公告)日：2006-04-20

申请号：US11253541

申请日：2005-10-19

Applicant: Jeong-gun Lee ,  Young-nam Kwon ,  Young-a Kim ,  Myo-yong Lee ,  Shin-i Yoo ,  Yeon-ja Cho ,  Kwang-ho Cheong ,  Chang-eun Yoo ,  Seung-yeon Yang

Inventor： Jeong-gun Lee ,  Young-nam Kwon ,  Young-a Kim ,  Myo-yong Lee ,  Shin-i Yoo ,  Yeon-ja Cho ,  Kwang-ho Cheong ,  Chang-eun Yoo ,  Seung-yeon Yang

IPC: C12N1/06

CPC classification number: C12M47/06 ,  B01F11/0266 ,  B01F13/0059 ,  B01L3/502761 ,  B01L2300/0816 ,  C12N1/066 ,  C12N13/00

Abstract: A method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser are provided. According to the method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser, cell lysis within 40 seconds is possible, the apparatus can be miniaturized using a laser diode, a DNA purification step can be directly performed after a disruption of cells or viruses, and a solution containing DNA can be transferred to a subsequent step after cell debris and beads to which inhibitors of a subsequent reaction are attached are removed with an electromagnet. In addition, by means of the cell lysis chip, an evaporation problem is solved, vibrations can be efficiently transferred to cells through magnetic beads, a microfluidics problem on a rough surface is solved by hydrophobically treating the inner surface of the chip, and the cell lysis chip can be applied to LOC.

Abstract translation: 提供了使用微磁珠和激光快速破坏细胞或病毒的方法和装置。 根据使用微磁珠和激光快速破坏细胞或病毒的方法和装置，可以在40秒内进行细胞裂解，使用激光二极管可以使装置小型化，DNA纯化步骤可以直接在 细胞或病毒的破坏以及含有DNA的溶液可以转移到随后的步骤中，随后的反应的抑制剂与细胞碎片和珠子一起用电磁铁去除。 另外，通过细胞裂解芯片，解决了蒸发问题，可以通过磁珠有效地将振动传递到细胞，粗糙表面上的微流体问题通过疏水处理芯片的内表面和电池来解决 裂解芯片可应用于LOC。