DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi
    30.
    发明授权
    DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi 有权
    用于在丝状真菌中分泌多肽的DNA序列,载体和融合多肽

    公开(公告)号:US06590078B2

    公开(公告)日:2003-07-08

    申请号:US09912733

    申请日:2001-07-23

    Abstract: The invention includes novel fusion nucleic acids encoding fusion polypeptides which when expressed in a filamentous fungus result in the expression of fusion polypeptides. The fusion nucleic acids comprise four nucleic acids which encode a fusion polypeptide comprising first, second, third and fourth amino acid sequences. The first nucleic acid encodes a signal polypeptide functional as a secretory sequence in a first filamentous fungus. The second nucleic acid encodes a secreted polypeptide or functional portion thereof which is normally secreted from the same filamentous fungus or a second filamentous fungus. The third nucleic acid encodes a cleavable linker while the fourth nucleic acid comprises at least two nucleic acids encoding desired polypeptides.

    Abstract translation: 本发明包括编码融合多肽的新型融合核酸,其在丝状真菌中表达时导致融合多肽的表达。 融合核酸包含编码包含第一,第二,第三和第四氨基酸序列的融合多肽的四个核酸。 第一核酸编码在第一丝状真菌中作为分泌序列起作用的信号多肽。 第二核酸编码通常由相同的丝状真菌或第二丝状真菌分泌的分泌的多肽或其功能部分。 第三核酸编码可切割的接头,而第四核酸包含编码所需多肽的至少两个核酸。

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