公开(公告)号：US20250043331A1

公开(公告)日：2025-02-06

申请号：US18918205

申请日：2024-10-17

Applicant: University of Washington

Inventor： Georg Seelig ,  Richard Muscat ,  Alexander B. Rosenberg

IPC: C12Q1/6806 ,  C12N15/10 ,  C12Q1/6855

Abstract: Methods of uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs, cDNAs, DNAs, proteins, peptides, and/or antigens.

公开(公告)号：US20250012619A1

公开(公告)日：2025-01-09

申请号：US18763337

申请日：2024-07-03

Applicant: University of Washington

Inventor： Joshua R. Smith ,  Jared Nakahara

IPC: G01G5/00 ,  G01G23/01 ,  G01G23/06 ,  G01N29/22

Abstract: Acoustic balances configured for weighing in ultrasonic non-contact manipulators, and associated systems and methods are described. In one embodiment, a method for a non-contact acoustic determination of a mass of an object includes capturing the object within an acoustic trap of an acoustic balance. The method also includes, in response to changing at least one acoustic parameter of the acoustic balance, changing an equilibrium position of the object; and in response to changing the equilibrium position of the object, causing the object to oscillate. The method also includes determining a resonant frequency of oscillation of the object; and based on the resonant frequency of oscillation of the object, determining the mass of the object.

公开(公告)号：US20250011866A1

公开(公告)日：2025-01-09

申请号：US18626712

申请日：2024-04-04

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

Inventor： Jesse Salk ,  Lawrence A. Loeb ,  Michael Schmitt

IPC: C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand. This method uniquely capitalizes on the redundant information stored in double-stranded DNA, thus overcoming technical limitations of prior methods utilizing data from only one of the two strands.

公开(公告)号：US20250011835A1

公开(公告)日：2025-01-09

申请号：US18887080

申请日：2024-09-17

Applicant: University of Washington

Inventor： Georg Seelig ,  Richard Muscat ,  Alexander B. Rosenberg

IPC: C12Q1/6806 ,  C12N15/10 ,  C12Q1/6855

Abstract: Methods of uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs, cDNAs, DNAs, proteins, peptides, and/or antigens.

公开(公告)号：US12191631B2

公开(公告)日：2025-01-07

申请号：US17054245

申请日：2019-05-10

Applicant: University of Washington

Inventor： Lih-Yuan Lin ,  Chen Zou

IPC: H01S5/12 ,  H01S5/042 ,  H01S5/183 ,  H01S5/187

Abstract: Within examples, a laser includes a first electrode and a second electrode; a first transport layer and a second transport layer that are between the first electrode and the second electrode; a gain layer positioned between the first transport layer and the second transport layer, where the gain layer comprises a material having a Perovskite crystal structure; and a substrate on which the first electrode, the second electrode, the first transport layer, the second transport layer, and the gain layer are formed, where a distributed feedback (DFB) waveguide is formed within the first transport layer, and where the laser is configured such that a current flowing through the gain layer between the first electrode and the second electrode causes the gain layer to emit coherent light. Examples also include methods for fabricating the laser, as well as additional lasers and methods for forming those lasers.

公开(公告)号：US12165387B2

公开(公告)日：2024-12-10

申请号：US17543224

申请日：2021-12-06

Applicant: The Boeing Company ,  University of Washington

Inventor： Adriana W. Blom-Schieber ,  Wei Guo ,  Ashis G. Banerjee ,  Ekta U. Samani

IPC: G06T5/40 ,  G06N3/08 ,  G06T3/4023 ,  G06T5/92 ,  G06T7/00 ,  G06T7/73 ,  G06V10/10 ,  G06V10/32 ,  G06V10/764 ,  G06V10/774 ,  G06V10/80 ,  G06V10/82

Abstract: A method of inspecting a composite structure formed of plies of tows is provided. The method involves receiving an image of an upper ply overlapping lower plies, the upper ply tow ends defining a boundary between plies, and applying extracted sub-images to a trained machine learning model to detect the upper or lower ply. Probability maps are produced in which pixels of the sub-images are associated with probabilities the pixels belong to an object class for the upper or lower ply. The method may also involve transforming the probability maps into reconstructed sub-images, stitching together a composite image, and applying the composite image to a feature detector to detect locations of tow ends of the upper ply. The method may also involve comparing the locations to as-designed locations of the tow ends, inspecting the composite structure, and indicating a result of the comparison.

公开(公告)号：US20240401108A1

公开(公告)日：2024-12-05

申请号：US18649007

申请日：2024-04-29

Applicant: UNIVERSITY OF WASHINGTON

Inventor： Scott R. Kennedy ,  Jesse J. Salk ,  Michael Hipp ,  Elizabeth Schmidt ,  Rosa Ana Risques ,  Daniela Nachmanson

IPC: C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/686

Abstract: The present technology relates generally to methods and compositions for targeted nucleic acid sequence enrichment, as well as uses of such enrichment for error-corrected nucleic acid sequencing applications. In some embodiments, highly accurate, error corrected and massively parallel sequencing of nucleic acid material is possible using a combination of uniquely labeled strands in a double-stranded nucleic acid complex in such a way that each strand can be informatically related to its complementary strand, but also distinguished from it following sequencing of each strand or an amplified product derived therefrom. In various embodiments, this information can be used for the purpose of error correction of the determined sequence.

公开(公告)号：US20240399134A1

公开(公告)日：2024-12-05

申请号：US18411651

申请日：2024-01-12

Applicant: University of Washington

Inventor： Barry Fulkerson ,  Jeremy Barribeau ,  Anna Galperin

IPC: A61M39/10 ,  A61B5/15 ,  A61M1/36 ,  A61M39/14 ,  A61M39/26

Abstract: Fluid access devices include a machine-side hydraulic circuit and a patient-side hydraulic circuit, and are configurable between a connected state and at least one disconnected state. In the connected state, fluid flows between the machine-side hydraulic circuit and the patient-side hydraulic circuit. In the disconnected state, fluid does not flow between the machine-side hydraulic circuit and the patient-side hydraulic circuit. In some disconnected states, fluid recirculates through at least one of the machine-side hydraulic circuit or the patient-side hydraulic circuit in the disconnected state.

公开(公告)号：US12157018B2

公开(公告)日：2024-12-03

申请号：US16712680

申请日：2019-12-12

Applicant: University of Washington ,  KONINKLIJKE PHILIPS N.V., A CORPORPORATION ORGANIZED AND EXISTING UNDER THE LAWS OF KINGDOM OF THE NETHERLANDS

Inventor： Vera Khokhlova ,  Michael R. Bailey ,  Navid Farr ,  Tatiana D. Khokhlova ,  Wayne Kreider ,  Adam D. Maxwell ,  Ari Partanen ,  Oleg A. Sapozhnikov ,  George R. Schade ,  Yak-Nam Wang

IPC: A61N7/02 ,  A61B17/22

Abstract: An example method includes generating an acoustic ultrasound wave that is focused at a focal point. The method further includes sequentially directing the focal point upon distinct portions of an object to form respective shock waves at the distinct portions of the object. The method further includes, via the respective shock waves, causing the distinct portions of the object to boil and form respective vapor cavities. The method further includes causing substantially uniform ablation of a region of the object that comprises the distinct portions. The substantially uniform ablation is caused via interaction of the respective shock waves with the respective vapor cavities. An example ablation system and an example non-transitory computer-readable medium, both related to the example method, are also disclosed.

公开(公告)号：US20240384221A1

公开(公告)日：2024-11-21

申请号：US18658657

申请日：2024-05-08

Applicant: University of Washington

Inventor： David Baker ,  Lukas F. Milles ,  Basile I.M. Wicky

IPC: C12M1/36 ,  B01D15/34 ,  C07K1/16 ,  C12N15/70 ,  C12P21/00 ,  G01N35/00 ,  G01N35/10

Abstract: Provided are systems, modules, compositions, and methods comprising integrated and modular workflows effective for high-throughput production and characterization of target polypeptides cost-effective protein production on the milligram scale, enabling characterization of hundreds of designs per day in a standardized format.