公开(公告)号：US20080152534A1

公开(公告)日：2008-06-26

申请号：US11643426

申请日：2006-12-21

Applicant: Jingwu Zhang ,  Xing Su ,  Lei Sun ,  Haovu Qin

Inventor： Jingwu Zhang ,  Xing Su ,  Lei Sun ,  Haovu Qin

IPC: C01B35/04 ,  C22C21/00 ,  C22C5/02 ,  C22C5/04 ,  C22C5/06 ,  C22C9/00

CPC classification number: C22C21/00 ,  C22C5/02 ,  C22C5/04

Abstract: Raman-active nanoclusters comprised of a metal and a Raman-active organic molecule incorporated therein that are capable of self-assembly are described. The Raman-active nanoclusters are capable of acting as sensitive reporters for analyte detection. A metal that enhances the Raman signal from the organic Raman-active compound is inherent in the nanocluster. A variety of organic Raman-active compounds and mixtures of compounds can be incorporated into the nanocluster.

Abstract translation: 描述了由能够自组装的金属和拉曼活性有机分子组成的拉曼活性纳米团簇。 拉曼活性纳米团簇能够作为分析物检测的敏感报告者。 从有机拉曼活性化合物增强拉曼信号的金属在纳米簇中是固有的。 可以将多种有机拉曼活性化合物和化合物的混合物掺入纳米团簇中。

公开(公告)号：US07361410B2

公开(公告)日：2008-04-22

申请号：US10940698

申请日：2004-09-13

Applicant: Jingwu Zhang ,  Xing Su ,  Lei Sun

Inventor： Jingwu Zhang ,  Xing Su ,  Lei Sun

IPC: B32B5/16 ,  B05D3/02

CPC classification number: G01N21/658 ,  G01N2021/653 ,  G01N2021/655 ,  G01N2021/656 ,  Y10S977/773 ,  Y10S977/777 ,  Y10S977/783 ,  Y10T428/12049 ,  Y10T428/2991 ,  Y10T428/2998

Abstract: Modified and functionalized metallic nanoclusters capable of providing an enhanced Raman signal from an organic Raman-active molecule incorporated therein are provided. For example, modifications include coatings and layers, such as adsorption layers, metal coatings, silica coatings, and organic layers. The nanoclusters are generally referred to as COINs (composite organic inorganic nanoparticles) and are capable of acting as sensitive reporters for analyte detection. A metal that enhances the Raman signal from the organic Raman-active compound is inherent in the nanocluster. A variety of organic Raman-active compounds and mixtures of compounds can be incorporated into the nanocluster.

Abstract translation: 提供了能够提供来自结合在其中的有机拉曼活性分子的增强拉曼信号的改性和官能化的金属纳米团簇。 例如，修饰包括涂层和层，例如吸附层，金属涂层，二氧化硅涂层和有机层。 纳米团簇通常被称为COIN（复合有机无机纳米粒子），并且能够作为敏感的报告物进行分析物检测。 从有机拉曼活性化合物增强拉曼信号的金属在纳米簇中是固有的。 可以将多种有机拉曼活性化合物和化合物的混合物掺入纳米团簇中。

公开(公告)号：US20070279626A9

公开(公告)日：2007-12-06

申请号：US10916710

申请日：2004-08-11

Applicant: Lei Sun ,  Xing Su

Inventor： Lei Sun ,  Xing Su

IPC: G01J3/44 ,  G01N21/65

CPC classification number: G01N33/54346 ,  G01N21/6428 ,  G01N21/6458 ,  G01N21/658 ,  G01N2021/6482 ,  G01N2021/653 ,  G01N2021/655 ,  G01N2021/656

Abstract: Methods and devices for solution-based detection of molecular and cellular analytes in a sample using composite organic-inorganic nanoclusters (COINs) are provided. The nanoclusters include metallic colloids and a Raman-active organic compound. A metal that enhances the Raman signal from the organic compound is inherent in the nanoparticle. Since a wide variety of Raman-active organic compounds can be incorporated into the particle, highly parallel analyte detection can be performed.

Abstract translation: 提供了使用复合有机 - 无机纳米团簇（COINs）的样品中分子和细胞分析物溶液检测的方法和装置。 纳米团簇包括金属胶体和拉曼活性有机化合物。 增强来自有机化合物的拉曼信号的金属是纳米颗粒中固有的。 由于可以将多种拉曼活性有机化合物掺入到颗粒中，因此可以进行高度平行的分析物检测。

公开(公告)号：US07291466B2

公开(公告)日：2007-11-06

申请号：US11111308

申请日：2005-04-20

Applicant: Xing Su ,  Selena Chan ,  Tae-Woong Koo ,  Mineo Yamakawa ,  Andrew A. Berlin

Inventor： Xing Su ,  Selena Chan ,  Tae-Woong Koo ,  Mineo Yamakawa ,  Andrew A. Berlin

IPC: C12Q1/68

CPC classification number: C12Q1/6825 ,  B82Y5/00 ,  G01N33/54373 ,  G01N2800/52 ,  Y10S977/924 ,  C12Q2565/501

Abstract: The present methods and apparatus concern the detection and/or identification of target analytes using probe molecules. In various embodiments of the invention, the probes or analytes are attached to one or more cantilevers. Binding of a probe to an analyte results in deflection of the cantilever, detected by a detection unit. A counterbalancing force may be applied to restore the cantilever to its original position. The counterbalancing force may be magnetic, electrical or radiative. The detection unit and the mechanism generating the counterbalancing force may be operably coupled to an information processing and control unit, such as a computer. The computer may regulate a feedback loop that maintains the cantilever in a fixed position by balancing the deflecting force and the counterbalancing force. The concentration of analytes in a sample may be determined from the magnitude of the counterbalancing force required to maintain the cantilever in a fixed position.

Abstract translation: 本方法和装置涉及使用探针分子检测和/或鉴定目标分析物。 在本发明的各种实施方案中，探针或分析物附着到一个或多个悬臂。 将探针与分析物结合导致悬臂的偏转，由检测单元检测。 可以应用平衡力将悬臂恢复到其原始位置。 平衡力可以是磁性的，电的或辐射的。 生成平衡力的检测单元和机构可以可操作地耦合到诸如计算机的信息处理和控制单元。 计算机可以通过平衡偏转力和平衡力来调节将悬臂维持在固定位置的反馈回路。 样品中分析物的浓度可以从将悬臂维持在固定位置所需的平衡力的大小来确定。

公开(公告)号：US07214490B2

公开(公告)日：2007-05-08

申请号：US10654281

申请日：2003-09-02

Applicant: Xing Su ,  Shoulian Dong

Inventor： Xing Su ,  Shoulian Dong

IPC: C12Q1/68 ,  C12P19/34 ,  C07H21/04

CPC classification number: C12Q1/6855 ,  C12Q2565/501 ,  C12Q2521/107 ,  C12Q2521/319

Abstract: The presently claimed invention provides methods and kits for amplifying a target sequence from within a nucleic acid population. The presently claimed invention provides selection probes which are complementary to at least a portion of said target sequence and mechanisms for adding a probe sequence to the 3′ end of a target sequence that is hybridized to a selection probe. The added 3′ probe sequence and a probe sequence added at the 5′ end of the target by adaptor ligation allow for selective amplification of the target sequence.

Abstract translation: 本发明提供了用于从核酸群体内扩增靶序列的方法和试剂盒。 目前要求保护的发明提供了与所述靶序列的至少一部分互补的选择探针和用于将探针序列添加到与选择探针杂交的靶序列的3'末端的机制。 添加的3'探针序列和通过衔接子连接在靶的5'端添加的探针序列允许靶序列的选择性扩增。

公开(公告)号：US20070048797A1

公开(公告)日：2007-03-01

申请号：US11527895

申请日：2006-09-26

Applicant: Xing Su

Inventor： Xing Su

IPC: G01N33/53 ,  C12Q1/37

CPC classification number: G01N33/54373 ,  G01N21/658 ,  G01N33/573 ,  G01N33/587

Abstract: Metallic nanoclusters capable of providing an enhanced Raman signal from an organic Raman-active molecule incorporated therein are provided. The nanoclusters are generally referred to as COINs (composite organic inorganic nanoparticles) and are capable of acting as sensitive reporters for analyte detection. Embodiments of the invention provide methods for detecting and quantitating enzyme activity. Further, the parallel assay capabilities of COINs allow libraries of compounds and molecules to be tested for enzyme activity.

Abstract translation: 提供能够从其中并入的有机拉曼活性分子提供增强的拉曼信号的金属纳米团簇。 纳米团簇通常被称为COIN（复合有机无机纳米粒子），并且能够作为敏感的报告物进行分析物检测。 本发明的实施方案提供了检测和定量酶活性的方法。 此外，COINs的平行测定能力允许化合物和分子的文库测试酶活性。

公开(公告)号：US20060281119A1

公开(公告)日：2006-12-14

申请号：US11446273

申请日：2006-06-02

Applicant: Selena Chan ,  Xing Su ,  Mineo Yamakawa

Inventor： Selena Chan ,  Xing Su ,  Mineo Yamakawa

IPC: C12Q1/68 ,  C12M1/34

CPC classification number: C12Q1/6816 ,  Y10S977/704 ,  Y10S977/728 ,  Y10S977/734 ,  Y10S977/742 ,  Y10S977/773 ,  Y10S977/774 ,  C12Q2565/601 ,  C12Q2563/155 ,  C12Q2537/143

Abstract: The methods, apparatus and compositions disclosed herein concern the detection, identification and/or sequencing of biomolecules, such as nucleic acids or proteins. In certain embodiments of the invention, coded probes comprising a probe molecule attached to one or more nano-barcodes may be allowed to bind to one or more target molecules. After binding and separation from unbound coded probes, the bound coded probes may be aligned on a surface and analyzed by scanning probe microscopy. The nano-barcodes may be any molecule or complex that is distinguishable by SPM, such as carbon nanotubes, fullerenes, submicrometer metallic barcodes, nanoparticles or quantum dots. Where the probes are oligonucleotides, adjacent coded probes hybridized to a target nucleic acid may be ligated together before alignment and SPM analysis. Compositions comprising coded probes are also disclosed herein. Systems for biomolecule analysis may comprise an SPM instrument and at least one coded probe attached to a surface.

Abstract translation: 本文公开的方法，装置和组合物涉及生物分子如核酸或蛋白质的检测，鉴定和/或测序。 在本发明的某些实施方案中，包含与一个或多个纳米条形码连接的探针分子的编码探针可以被允许与一个或多个靶分子结合。 在结合和分离未结合的编码探针之后，结合编码的探针可以在表面上对准并通过扫描探针显微镜进行分析。 纳米条形码可以是通过SPM可区分的任何分子或复合物，例如碳纳米管，富勒烯，亚微米金属条形码，纳米粒子或量子点。 当探针是寡核苷酸时，与靶核酸杂交的相邻编码探针可以在对准和SPM分析之前连接在一起。 包含编码探针的组合物也在本文中公开。 用于生物分子分析的系统可以包括SPM仪器和附接到表面的至少一个编码探针。

公开(公告)号：US20060100787A1

公开(公告)日：2006-05-11

申请号：US10985547

申请日：2004-11-09

Applicant: Andrew Berlin ,  Joseph Kosmoski ,  Narayanan Sundararajan ,  Xing Su ,  Mineo Yamakawa

Inventor： Andrew Berlin ,  Joseph Kosmoski ,  Narayanan Sundararajan ,  Xing Su ,  Mineo Yamakawa

IPC: G06F19/00

CPC classification number: G01Q70/18 ,  G01Q60/16 ,  G01Q60/42

Abstract: Methods for making nanocodes that can be detected using scanning probe microscopy are provided, as are nanocodes constructed of two or more polymers, including homogeneous polymers such as nucleic acid molecules and heterogeneous polymers such as peptide nucleic acid polymers, and subunits useful for constructing such nanocodes. Also provided are modified nanocodes such as a nanocode containing one or more linked metals such as gold or iron and/or a linked probe that can specifically bind a target molecule. In addition, systems are provided that include such nanocodes, for example, a system that includes the nanocode and a surface and/or a scanning probe microscope probe. Methods of using such nanocodes, for example, to detect and/or identify a target molecule in a sample (e.g., a biological or environmental sample) using scanning probe microscopy, also are provided.

Abstract translation: 提供了可以使用扫描探针显微镜检测的纳代码的方法，以及由两种或更多种聚合物构成的纳代码，包括均质聚合物，例如核酸分子和非均相聚合物如肽核酸聚合物，以及可用于构建这样的纳代码的亚基 。 还提供了修饰的纳代码，例如包含一种或多种连接的金属如金或铁的纳代码和/或可以特异性结合靶分子的连接的探针。 此外，提供了包括这样的纳代码的系统，例如，包括纳代码和表面和/或扫描探针显微镜探针的系统。 还提供了使用这样的纳代码例如使用扫描探针显微镜检测和/或鉴定样品（例如，生物或环境样品）中的靶分子的方法。

公开(公告)号：US20060046313A1

公开(公告)日：2006-03-02

申请号：US11027470

申请日：2004-12-30

Applicant: Mark Roth ,  Tae-Woong Koo ,  Andrew Berlin ,  Selena Chan ,  Xing Su ,  Lei Sun

Inventor： Mark Roth ,  Tae-Woong Koo ,  Andrew Berlin ,  Selena Chan ,  Xing Su ,  Lei Sun

IPC: G01N33/543

CPC classification number: G01N33/54366 ,  G01N33/54373 ,  G01N33/58 ,  Y10T436/10

Abstract: Methods and apparatus are provided for assaying cell samples, which may be living cells, using probes labeled with composite organic-inorganic nanoparticles (COINs) and microspheres with COINs embedded within a polymer matrix to which the probe moiety is attached. COINs intrinsically produce SERS signals upon laser irradiation, making COIN-labeled probes particularly suitable in a variety of methods for assaying cells, including biological molecules that may be contained on or within cells, most of which are not inherently Raman-active. The invention provides variations of the sandwich immunoassay employing both specific and degenerate binding, methods for reverse phase assay of tissue samples and cell microstructures, in solution displacement and competition assays, and the like. Systems and chips useful for practicing the invention assays are also provided.

Abstract translation: 提供了用于使用用复合有机 - 无机纳米颗粒（COIN）标记的探针和嵌入探针部分所连接的聚合物基质内的COIN的微球来测定可能为活细胞的细胞样品的方法和装置。 COIN在激光照射下固有地产生SERS信号，使得COIN标记的探针特别适用于多种测定细胞的方法，包括可能包含在细胞内或细胞内的生物分子，其中大部分不是固有的拉曼活性的。 本发明提供使用特异性和简并结合的夹心免疫测定的变体，组织样品和细胞微结构的反相测定方法，溶液置换和竞争测定等。 还提供了用于实施本发明测定的系统和芯片。

公开(公告)号：US20060046311A1

公开(公告)日：2006-03-02

申请号：US10927996

申请日：2004-08-26

Applicant: Lei Sun ,  Xing Su ,  Tae-Woong Koo ,  Selena Chan

Inventor： Lei Sun ,  Xing Su ,  Tae-Woong Koo ,  Selena Chan

IPC: G01N33/543

CPC classification number: G01N33/58 ,  G01N33/54366 ,  G01N33/54373

Abstract: Methods and apparatus are provided herein for assaying biological samples using probes labeled with composite organic-inorganic nanoparticles (COINs) and microspheres with COINs embedded within a polymer matrix to which the probe moiety is attached. COINs are Raman-active nanoparticles made up of aggregated primary metal crystal particles with Raman-active organic compounds adsorbed on the surface in the junctions of aggregated primary metal crystal particles or embedded in the crystal lattice of the primary metal particles. Since COINs intrinsically produce SERS signals upon laser irradiation, COIN-labeled probes are particularly suitable in a variety of methods for assaying biological molecules, most of which are not inherently Raman-active. The invention provides variations of the sandwich immunoassay employing both specific and degenerate binding, methods for reverse phase assay of tissue samples and cell microstructures, in solution displacement and competition assays, and the like. Kits and chips useful for practicing the invention assays are also provided.

Abstract translation: 本文提供了用于使用用复合有机 - 无机纳米颗粒（COIN）标记的探针和具有嵌入探针部分所连接的聚合物基质内的COIN的微球来测定生物样品的方法和装置。 COIN是由聚集的初级金属晶体颗粒组成的拉曼活性纳米颗粒，其中拉曼活性有机化合物吸附在聚集的初级金属晶体颗粒的接合处的表面上或嵌入在一次金属颗粒的晶格中。 由于COIN在激光照射下固有地产生SERS信号，所以COIN标记的探针特别适用于分析生物分子的多种方法，其中大部分不是固有的拉曼活性的。 本发明提供使用特异性和简并结合的夹心免疫测定的变体，组织样品和细胞微结构的反相测定方法，溶液置换和竞争测定等。 还提供了可用于实施本发明测定的试剂盒和碎片。