公开(公告)号：US07816519B2

公开(公告)日：2010-10-19

申请号：US11560579

申请日：2006-11-16

Applicant: Zhenjun Diwu ,  Jixiang Liu ,  Kyle Gee

Inventor： Zhenjun Diwu ,  Jixiang Liu ,  Kyle Gee

IPC: C07D471/04 ,  C07D491/04 ,  C07D495/04 ,  C07D495/22 ,  G01N33/58

CPC classification number: G01N33/582 ,  C07D471/04 ,  C07D491/04 ,  C07D491/044 ,  C07D491/14 ,  C07D491/147 ,  C07D491/22 ,  C07D495/04 ,  C07D495/14 ,  C07D495/22 ,  C07D498/14 ,  C12Q1/6816 ,  Y10S436/80 ,  Y10T436/141111 ,  Y10T436/143333 ,  Y10T436/145555 ,  C12Q2563/107

Abstract: The present invention describes novel dyes, including coumarins, rhodamines, and rhodols that incorporate additional fused aromatic rings. The dyes of the invention absorb at a longer wavelength than structurally similar dyes that do not possess the fused aromatic rings. Many of the dyes of the invention are useful fluorescent dyes. The invention includes chemically reactive dyes, dye-conjugates, and the use of such dyes in staining samples and detecting ligands or other analytes.

Abstract translation: 本发明描述了新颖的染料，包括掺入另外的稠合芳环的香豆素，罗丹明和罗丹醇。 本发明的染料比不具有稠合芳环的结构相似的染料长的波长吸收。 本发明的许多染料是有用的荧光染料。 本发明包括化学活性染料，染料共轭物，以及这些染料在染色样品和检测配体或其他分析物中的用途。

公开(公告)号：US20100255504A1

公开(公告)日：2010-10-07

申请号：US12726267

申请日：2010-03-17

Applicant: Zhenjun Diwu ,  Timothy Dubrovsky ,  Barnaby Abrams ,  Jinfang Liao ,  Qinglin Meng

Inventor： Zhenjun Diwu ,  Timothy Dubrovsky ,  Barnaby Abrams ,  Jinfang Liao ,  Qinglin Meng

IPC: G01N33/53 ,  C07K16/00 ,  C07D405/10 ,  C07D417/04 ,  C07D405/04 ,  C07D401/12

CPC classification number: C07D405/04 ,  C07D405/14 ,  C07D409/04 ,  C07D409/14 ,  C07D417/04 ,  C07D417/14 ,  C09B57/02 ,  Y10T436/13

Abstract: Chemically-reactive, water-soluble, heterocycle-substituted 7-hydroxycoumarin dyes, their bioconjugates and uses are described. The conjugates derived from reactive heterocycle-substituted 7-hydroxycoumarin dyes are used for analyzing biological compounds. These heterocycle-substituted 7-hydroxycoumarin dyes are particularly useful as fluorescent labels for biopolymer detection reagents, such as antibodies or nucleic acid probes. The dye-antibody conjugates of the invention are particularly useful for analyzing analytes using a flow cytometer equipped with a violet laser as an excitation source due to their strong absorption at 405 nm and high fluorescence quantum yield.

Abstract translation: 描述了化学反应性，水溶性，杂环取代的7-羟基香豆素染料，它们的生物共轭物和用途。 衍生自反应性杂环取代的7-羟基香豆素染料的共轭物用于分析生物化合物。 这些杂环取代的7-羟基香豆素染料特别可用作生物聚合物检测试剂如抗体或核酸探针的荧光标记。 本发明的染料 - 抗体缀合物由于其在405nm下的强吸收和高荧光量子产率而特别适用于使用配有紫激光作为激发源的流式细胞仪分析分析物。

公开(公告)号：US20100022768A1

公开(公告)日：2010-01-28

申请号：US12583496

申请日：2009-08-20

Applicant: Zhenjun Diwu ,  Jianheng Zhang ,  Yi Tang ,  Xiang Guobing

Inventor： Zhenjun Diwu ,  Jianheng Zhang ,  Yi Tang ,  Xiang Guobing

IPC: C07D487/14

CPC classification number: C09B23/06 ,  C07D487/14 ,  C07D487/22 ,  C07D498/22 ,  C09B23/0066 ,  C09B23/02 ,  G01N33/52 ,  G01N33/533 ,  Y10T436/143333

Abstract: Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and 3′-position, their bioconjugates and their uses are described. 1,3′-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1′-crosslinked or non-crosslinked dyes. The invention includes derivative compounds having one or more benzo nitrogens.

Abstract translation: 描述了在1位和3'-位之间分子内交联的化学反应性碳菁染料，它们的生物缀合物及其用途。 1,3'-交联的碳氰酸酯优于光谱相似的1,1'-交联或非交联染料的共轭物。 本发明包括具有一个或多个苯并氮的衍生化合物。

公开(公告)号：US20090258418A1

公开(公告)日：2009-10-15

申请号：US12287071

申请日：2008-10-06

Applicant: Zhenjun Diwu ,  Jianheng Zhang ,  Yi Tang ,  Xiang Guobing

Inventor： Zhenjun Diwu ,  Jianheng Zhang ,  Yi Tang ,  Xiang Guobing

IPC: C12N5/06 ,  C07K2/00 ,  C07H21/00 ,  C07H7/06 ,  C12N1/00

CPC classification number: C09B23/06 ,  C07D487/14 ,  C07D487/22 ,  C07D498/22 ,  C09B23/0066 ,  C09B23/02 ,  G01N33/52 ,  G01N33/533 ,  Y10T436/143333

Abstract: Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and 3′-position, their bioconjugates and their uses are described. 1,3′-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1′-crosslinked or non-crosslinked dyes. The invention includes derivative compounds having one or more benzo nitrogens.

Abstract translation: 描述了在1位和3'-位之间分子内交联的化学反应性碳菁染料，它们的生物缀合物及其用途。 1,3'-交联的碳氰酸酯优于光谱相似的1,1'-交联或非交联染料的共轭物。 本发明包括具有一个或多个苯并氮的衍生化合物。

公开(公告)号：US20090215108A1

公开(公告)日：2009-08-27

申请号：US12287090

申请日：2008-10-06

Applicant: Zhenjun Diwu ,  Jianheng Zhang ,  Yi Tang ,  Xiang Guobing

Inventor： Zhenjun Diwu ,  Jianheng Zhang ,  Yi Tang ,  Xiang Guobing

IPC: C12Q1/02 ,  G01N33/00

CPC classification number: C09B23/06 ,  C07D487/14 ,  C07D487/22 ,  C07D498/22 ,  C09B23/0066 ,  C09B23/02 ,  G01N33/52 ,  G01N33/533 ,  Y10T436/143333

Abstract: Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and 3′-position, their bioconjugates and their uses are described. 1,3′-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1′-crosslinked or non-crosslinked dyes. The invention includes derivative compounds having one or more benzo nitrogens.

Abstract translation: 描述了在1位和3'-位之间分子内交联的化学反应性碳菁染料，它们的生物缀合物及其用途。 1,3'-交联的碳氰酸酯优于光谱相似的1,1'-交联或非交联染料的共轭物。 本发明包括具有一个或多个苯并氮的衍生化合物。

公开(公告)号：US06800765B2

公开(公告)日：2004-10-05

申请号：US10108656

申请日：2002-03-27

Applicant: Zhenjun Diwu ,  Jesse J. Twu ,  Guoliang Yi ,  Luke D. Lavis ,  Yen-Wen Chen ,  Kelly J. Cassutt

Inventor： Zhenjun Diwu ,  Jesse J. Twu ,  Guoliang Yi ,  Luke D. Lavis ,  Yen-Wen Chen ,  Kelly J. Cassutt

IPC: C07D31182

CPC classification number: C07D493/10 ,  C07D311/82 ,  G01N1/30 ,  G01N33/84 ,  G01N2001/302 ,  G01N2800/04

Abstract: Systems, including compositions and methods, for measuring pH, particularly in cells, organelles, and other samples. The compositions include pH-sensitive fluorescent and fluorogenic 2′,7′-dialkylfluorescein derivatives and associated nonfluorescent precursor compounds. The compositions may permit ratiometric measurement in the excitation spectrum and the emission spectrum. The methods include adding a precursor compound to a sample cell, incubating the sample cell to release the free indicator, illuminating the sample cell, and detecting the fluorescence response of the free indicator.

Abstract translation: 用于测量pH的系统，包括组合物和方法，特别是在细胞，细胞器和其它样品中。 组合物包括pH敏感的荧光和荧光2'，7'-二烷基荧光素衍生物和相关的非荧光前体化合物。 组合物可以允许在激发光谱和发射光谱中进行比例测量。 所述方法包括向样品池中加入前体化合物，孵育样品池以释放游离指示物，照射样品池，以及检测游离指示剂的荧光响应。

公开(公告)号：US09097730B2

公开(公告)日：2015-08-04

申请号：US13374967

申请日：2012-01-25

Applicant: Zhenjun Diwu ,  Jinfang Liao

Inventor： Zhenjun Diwu ,  Jinfang Liao

IPC: G01N33/52 ,  G01N33/84 ,  C07D219/06 ,  C07D311/90 ,  C07D493/10 ,  G01N33/533

CPC classification number: G01N33/84 ,  C07C2603/24 ,  C07D219/06 ,  C07D311/90 ,  C07D493/10 ,  G01N33/533

Abstract: Fluorescent dyes useful for preparing fluorescent metal ion indicators, the fluorescent indicators themselves, and the use of the fluorescent indicators for the detection, discrimination and quantification of metal cations.

Abstract translation: 用于制备荧光金属离子指示剂的荧光染料，荧光指示剂本身，以及使用荧光指示剂检测，鉴别和定量金属阳离子。

公开(公告)号：US08999690B2

公开(公告)日：2015-04-07

申请号：US13374994

申请日：2012-01-27

Applicant: Zhenjun Diwu ,  Jianheng Zhang ,  Yi Tang ,  Xiang Guobing

Inventor： Zhenjun Diwu ,  Jianheng Zhang ,  Yi Tang ,  Xiang Guobing

IPC: C09B23/01 ,  C07D498/22 ,  C07D513/22 ,  G01N33/533 ,  C07D487/14 ,  C07D487/22 ,  C09B23/02

CPC classification number: C09B23/06 ,  C07D487/14 ,  C07D487/22 ,  C07D498/22 ,  C09B23/0066 ,  C09B23/02 ,  G01N33/52 ,  G01N33/533 ,  Y10T436/143333

Abstract: Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and 3′-position, their bioconjugates and their uses are described. 1,3′-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1′-crosslinked or non-crosslinked dyes. The invention includes derivative compounds having one or more benzo nitrogens.

Abstract translation: 描述了在1位和3'-位之间分子内交联的化学反应性碳菁染料，它们的生物缀合物及其用途。 1,3'-交联的碳氰酸酯优于光谱相似的1,1'-交联或非交联染料的共轭物。 本发明包括具有一个或多个苯并氮的衍生化合物。

公开(公告)号：US08623324B2

公开(公告)日：2014-01-07

申请号：US13181107

申请日：2011-07-12

Applicant: Timothy Dubrovsky ,  Barnaby Abrams ,  Zhenjun Diwu ,  Qinglin Meng ,  Jinfang Liao ,  Haitao Guo

Inventor： Timothy Dubrovsky ,  Barnaby Abrams ,  Zhenjun Diwu ,  Qinglin Meng ,  Jinfang Liao ,  Haitao Guo

IPC: A61K51/00 ,  A61M36/14

CPC classification number: C09B23/083 ,  C09B11/08 ,  C09B11/24 ,  C09B11/26 ,  C09B57/00 ,  C09B57/10 ,  G01N33/533

Abstract: Chemically reactive dyes that are intramolecularly crosslinked with a water-soluble bridge, their bioconjugates and their uses are described. Reactive fluorescent dyes that have a water-soluble bridge are superior to those of conjugates of spectrally non-crosslinked dyes or the dyes that are crosslinked with a hydrophobic bridge. The invention includes reactive fluorescent dyes, their biological conjugates and uses.

Abstract translation: 描述了与水溶性桥分子内交联的化学活性染料及其生物共轭物及其用途。 具有水溶性桥梁的活性荧光染料优于光谱非交联染料或与疏水桥交联的染料的共轭物。 本发明包括反应性荧光染料，它们的生物共轭物和用途。

公开(公告)号：US08426618B2

公开(公告)日：2013-04-23

申请号：US13374728

申请日：2012-01-09

Applicant: Zhenjun Diwu ,  Jianheng Zhang ,  Yi Tang

Inventor： Zhenjun Diwu ,  Jianheng Zhang ,  Yi Tang

IPC: C07D209/46 ,  C07D209/02

CPC classification number: C07D409/14 ,  C07D209/60 ,  C07D403/14 ,  C07D409/04 ,  C07D409/06 ,  C07D417/14 ,  C07D471/04 ,  C07H21/04 ,  C07J43/00 ,  G01N33/542 ,  G01N33/582

Abstract: The quenching compounds of the invention are weakly luminescent cyanines that are substituted by one or more heteroaromatic quenching moieties. The quenching compounds of the invention exhibit little or no observable luminescence and efficiently quench a broad spectrum of luminescent compounds. The chemically reactive quenching compounds possess utility for labeling a wide variety of substances, including biomolecules. These labeled substances are highly useful for a variety of energy-transfer assays and applications.