公开(公告)号：US08604180B2

公开(公告)日：2013-12-10

申请号：US11662358

申请日：2005-09-05

申请人： Hideaki Yukawa ,  Masayuki Inui

发明人： Hideaki Yukawa ,  Masayuki Inui

IPC分类号： C07H21/04 ,  C12N1/21

CPC分类号： C07K14/34 ,  C12N15/67

摘要： The present invention relates to a method of inducing expression of a promoter function of various genes in a Coryneform bacterium related to function exertion, in order to exert the function of a Coryneform bacterium highly and effectively under an anaerobic condition, for producing an organic compound useful under an anaerobic condition, more particularly, provides a method of enhancing and/or suppressing the promoter function related to various genes, for the purpose of highly and effectively expressing various protein genes necessary for production of an objective substance, and suppressing expression of an unnecessary protein gene.The DNA fragment of the present invention is useful as a primer which is introduced into a transformed Coryneform bacterium producing a useful substance such as lactic acid and succinic acid highly and at a high efficiency.

摘要翻译： 本发明涉及在与功能运用相关的棒状杆菌型细菌中诱导各种基因的启动子功能的表达的方法，以便在厌氧条件下高效且有效地发挥棒状细菌的功能，以产生有用的有机化合物 在厌氧条件下，更特别地，提供了增强和/或抑制与各种基因相关的启动子功能的方法，目的是高度有效地表达生产目标物质所必需的各种蛋白质基因，并抑制不必要的表达 蛋白质基因。 本发明的DNA片段可用作引入到转化棒状细菌中的引物，其能高效且高效地生产乳酸和琥珀酸等有用物质。

公开(公告)号：US09290770B2

公开(公告)日：2016-03-22

申请号：US14238527

申请日：2012-08-21

申请人： Hideaki Yukawa ,  Masayuki Inui

发明人： Hideaki Yukawa ,  Masayuki Inui

IPC分类号： C12N15/77 ,  C12P13/08 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10

CPC分类号： C12N15/77 ,  C12N9/0006 ,  C12N9/0016 ,  C12N9/1022 ,  C12P13/08 ,  C12Y101/01086 ,  C12Y104/01009 ,  C12Y202/01006 ,  Y02P20/52

摘要： A transformant obtainable by introducing one or more of the following DNAs (a), (b), and (c) into a coryneform bacterium as a host.(a) A DNA which encodes acetohydroxy acid synthase derived from Corynebacterium glutamicum and which has a mutation changing the glycine at position 156 to glutamic acid (G156E) in an amino acid sequence encoded by the DNA, or an analog thereof.(b) A DNA which encodes acetohydroxy acid isomeroreductase derived from Corynebacterium glutamicum and which has mutations changing the serine at position 34 to glycine (S34G), the leucine at position 48 to glutamic acid (L48E), and the arginine at position 49 to phenylalanine (R49F) in an amino acid sequence encoded by the DNA, or an analog thereof. (c) A DNA which encodes leucine dehydrogenase derived from Lysinibacillus sphaericus, or an analog thereof.

摘要翻译： 将一种或多种以下DNA（a），（b）和（c）引入作为宿主的棒状细菌获得的转化体。 （a）编码源自谷氨酸棒杆菌的乙酰羟酸合酶的DNA，其在DNA编码的氨基酸序列或其类似物中具有将位置156处的甘氨酸变为谷氨酸（G156E）的突变。 （b）编码源自谷氨酸棒杆菌的乙酰羟酸异构体还原酶的DNA，其具有将第34位的丝氨酸改变为甘氨酸（S34G），将第48位的亮氨酸变为谷氨酸（L48E），将第49位的精氨酸变为苯丙氨酸 （R49F），其由DNA编码的氨基酸序列或其类似物。 （c）编码源自嗜裂杆菌属的亮氨酸脱氢酶的DNA，或其类似物。

公开(公告)号：US08846367B2

公开(公告)日：2014-09-30

申请号：US13988107

申请日：2011-11-17

申请人： Hideaki Yukawa ,  Masayuki Inui

发明人： Hideaki Yukawa ,  Masayuki Inui

IPC分类号： C12N9/90 ,  C12N1/20 ,  C12P19/30 ,  C12P7/22 ,  C12N9/88 ,  C12N15/77

CPC分类号： C12N15/77 ,  C12N9/88 ,  C12P7/22 ,  C12Y401/99002

摘要： Provided is a phenol-producing transformant constructed by transferring a gene which encodes an enzyme having tyrosine phenol-lyase activity into a coryneform bacterium as a host. Also provided is a process for producing phenol, which comprises a step of allowing the transformant to react in a reaction mixture containing tyrosine, a salt thereof, or an ester thereof under reducing conditions, and a step of collecting phenol from the reaction mixture.

摘要翻译： 提供了通过将编码具有酪氨酸酚裂解酶活性的酶的基因转移到作为宿主的棒状细菌中构建的产生苯酚的转化体。 还提供了一种生产苯酚的方法，其包括在还原条件下使转化体在含有酪氨酸，其盐或其酯的反应混合物中反应的步骤和从反应混合物中收集苯酚的步骤。

公开(公告)号：US20130273624A1

公开(公告)日：2013-10-17

申请号：US13884549

申请日：2011-11-09

申请人： Hideaki Yukawa ,  Masayuki Inui

发明人： Hideaki Yukawa ,  Masayuki Inui

IPC分类号： C12N15/77 ,  C12P7/22

CPC分类号： C12N15/77 ,  C12N9/88 ,  C12P7/22 ,  Y02P20/52

摘要： Provided is a phenol-producing transformant constructed by transferring a gene which encodes an enzyme having chorismate-pyruvate lyase activity and a gene which encodes an enzyme having 4-hydroxybenzoate decarboxylase activity into a coryneform bacterium as a host. Also provided is a process for producing phenol, which comprises a step of allowing the transformant to react in a reaction mixture containing a saccharide under reducing conditions, and a step of collecting phenol from the reaction mixture.

摘要翻译： 本发明提供一种生产苯酚的转化体，其通过将编码具有4-硝基苯甲酸脱羧酶活性的酶的基因编码为具有佐罗沙普 - 丙酮酸裂解酶活性的酶的基因转移到作为宿主的棒状细菌中构建。 还提供了一种生产苯酚的方法，其包括使得转化体在还原条件下在含有糖的反应混合物中反应的步骤和从反应混合物中收集苯酚的步骤。

公开(公告)号：US20130267000A1

公开(公告)日：2013-10-10

申请号：US13988107

申请日：2011-11-17

申请人： Hideaki Yukawa ,  Masayuki Inui

发明人： Hideaki Yukawa ,  Masayuki Inui

IPC分类号： C12N15/77 ,  C12P7/22

CPC分类号： C12N15/77 ,  C12N9/88 ,  C12P7/22 ,  C12Y401/99002

摘要： Provided is a phenol-producing transformant constructed by transferring a gene which encodes an enzyme having tyrosine phenol-lyase activity into a coryneform bacterium as a host. Also provided is a process for producing phenol, which comprises a step of allowing the transformant to react in a reaction mixture containing tyrosine, a salt thereof, or an ester thereof under reducing conditions, and a step of collecting phenol from the reaction mixture.

摘要翻译： 提供了通过将编码具有酪氨酸酚裂解酶活性的酶的基因转移到作为宿主的棒状细菌中构建的产生苯酚的转化体。 还提供了一种生产苯酚的方法，其包括在还原条件下使转化体在含有酪氨酸，其盐或其酯的反应混合物中反应的步骤和从反应混合物中收集苯酚的步骤。

公开(公告)号：US08216820B2

公开(公告)日：2012-07-10

申请号：US12988882

申请日：2009-04-15

申请人： Hideaki Yukawa ,  Masayuki Inui

发明人： Hideaki Yukawa ,  Masayuki Inui

IPC分类号： C12N1/21 ,  C12P7/04

CPC分类号： C12N15/52 ,  C12P7/04 ,  C12R1/15

摘要： A transformant capable of producing isopropanol which is constructed by transferring the following genes (a) to (d) into a coryneform bacterium: (a) an exogenous gene which encodes an enzyme having acetyl-CoA acetyltransferase activity; (b) an exogenous gene which encodes an enzyme having acetoacetyl CoA:acetate CoA-transferase activity; (c) an exogenous gene which encodes an enzyme having acetoacetate decarboxylase activity; and (d) an exogenous gene which encodes an enzyme having isopropanol dehydrogenase activity.

摘要翻译： 能够产生异丙醇的转化体，其通过将下列基因（a）至（d）转移到棒状细菌中构建：（a）编码具有乙酰辅酶A乙酰转移酶活性的酶的外源基因; （b）编码具有乙酰乙酰CoA：乙酸CoA转移酶活性的酶的外源基因; （c）编码具有乙酰乙酸脱羧酶活性的酶的外源基因; 和（d）编码具有异丙醇脱氢酶活性的酶的外源基因。

公开(公告)号：US20120115196A1

公开(公告)日：2012-05-10

申请号：US13258670

申请日：2010-03-29

申请人： Hideaki Yukawa ,  Masayuki Inui

发明人： Hideaki Yukawa ,  Masayuki Inui

IPC分类号： C12P7/16 ,  C12N1/21

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/88 ,  Y02E50/10

摘要： A Corynebacterium glutamicum transformant having the capability of producing isobutanol and the following genes (1) to (5): (1) a gene which encodes an enzyme having acetohydroxy acid synthase activity; (2) a gene which encodes an enzyme having acetohydroxy acid isomeroreductase activity; (3) a gene which encodes an enzyme having dihydroxy acid dehydratase activity; (4) a gene which encodes an enzyme having 2-keto acid decarboxylase activity; and (5) a gene which encodes an enzyme having alcohol dehydrogenase activity, at least one of the genes being endogenous, and at least one of the genes being exogenous, efficiently produces isobutanol.

摘要翻译： 具有产生异丁醇能力的谷氨酸棒杆菌转化体和以下基因（1）至（5）：（1）编码具有乙酰羟酸合成酶活性的酶的基因; （2）编码具有乙酰羟酸异构体还原酶活性的酶的基因; （3）编码具有二羟基酸脱水酶活性的酶的基因; （4）编码具有2-酮酸脱羧酶活性的酶的基因; 和（5）编码具有醇脱氢酶活性的酶的基因，至少一个基因是内源的，并且至少一个外源基因有效地产生异丁醇。

公开(公告)号：US20070087423A1

公开(公告)日：2007-04-19

申请号：US10566129

申请日：2004-07-28

申请人： Shikiko Murakami ,  Kaori Nakata ,  Shohei Okino ,  Yuko Ikenaga ,  Masayuki Inui ,  Hideaki Yukawa

发明人： Shikiko Murakami ,  Kaori Nakata ,  Shohei Okino ,  Yuko Ikenaga ,  Masayuki Inui ,  Hideaki Yukawa

IPC分类号： C12P7/46 ,  C12N1/21

CPC分类号： C12R1/15 ,  C12P7/46

摘要： The present invention provides an aerobic coryneform bacterium transformant in which a lactate dehydrogenase gene is disrupted, and a pyruvate carboxylase gene is recombined so as to be highly expressed by a genetic engineering method. The aerobic coryneform bacterium transformant of the present invention can produce dicarboxylic acids from saccharides at a high production rate.

摘要翻译： 本发明提供一种有氧棒状细菌转化体，其中乳酸脱氢酶基因被破坏，并且丙酮酸羧化酶基因被重组以通过遗传工程方法高度表达。 本发明的有氧棒状细菌转化体可以高生产率从糖生产二羧酸。

公开(公告)号：US09327229B2

公开(公告)日：2016-05-03

申请号：US13701174

申请日：2011-05-31

申请人： Tatsuto Nagayasu ,  Takashi Kamijo ,  Masayuki Inui ,  Tsuyoshi Oishi ,  Hiromitsu Nagayasu ,  Hiroshi Tanaka ,  Takuya Hirata ,  Tatsuya Tsujiuchi ,  Susumu Okino ,  Naoyuki Kamiyama ,  Seiji Yoshihara

发明人： Tatsuto Nagayasu ,  Takashi Kamijo ,  Masayuki Inui ,  Tsuyoshi Oishi ,  Hiromitsu Nagayasu ,  Hiroshi Tanaka ,  Takuya Hirata ,  Tatsuya Tsujiuchi ,  Susumu Okino ,  Naoyuki Kamiyama ,  Seiji Yoshihara

IPC分类号： B01D53/75 ,  B01D53/14 ,  C01B31/20 ,  B01D53/50

摘要： SOx removal equipment 15 which reduces sulfur oxides from flue gas 12 from a boiler 11, a cooler 16 which is provided on the downstream side of the SOx removal equipment 15 so as to reduce the sulfur oxides from the flue gas and decrease a gas temperature, CO2 recovery equipment 17 which includes an absorber for bringing CO2 in the flue gas into contact with a CO2 absorption liquid so as to be reduced and a regenerator for causing the CO2 absorption liquid to emit CO2 so as to recover CO2 and regenerate the CO2 absorption liquid, and ammonia injection equipment 22 for reducing a mist generation material which is a generation source of mist that is generated in the absorber of the CO2 recovery equipment before introducing the flue gas to the CO2 recovery equipment, are included.

公开(公告)号：US20100240522A1

公开(公告)日：2010-09-23

申请号：US12382737

申请日：2009-03-23

申请人： Tomoyuki Inui ,  Masayuki Inui ,  Takuma Kimura ,  Mohammad Abdullah Al-Saleh ,  Halim Hamid Redhwi ,  Mohammad Ashraf Ali ,  Shakeel Ahmed

发明人： Tomoyuki Inui ,  Masayuki Inui ,  Takuma Kimura ,  Mohammad Abdullah Al-Saleh ,  Halim Hamid Redhwi ,  Mohammad Ashraf Ali ,  Shakeel Ahmed

IPC分类号： B01J37/30 ,  B01J21/16

CPC分类号： B01J37/0201 ,  B01J21/16 ,  B01J23/652 ,  B01J23/89 ,  B01J23/8913 ,  B01J35/1019 ,  B01J35/1023 ,  B01J35/1038 ,  B01J35/1042 ,  B01J37/0205 ,  B01J37/18 ,  C10G29/205 ,  C10G45/10 ,  C10G47/14

摘要： The catalyst exhibiting hydrogen spillover effect relates to the composition of a catalyst exhibiting hydrogen spillover effect and to a process for preparing the catalyst. The catalyst has a reduced transition base metal of Group VIB or Group VIIIB, such as cobalt, nickel, molybdenum or tungsten, supported on a high porous carrier, such as saponite, the base metal being ion-exchanged with at least one precious metal of Group VIIIB. The process includes the steps of loading the base metal onto the support, reducing the base metal, preferably with H2 at 600° C., and thereafter ion-exchanging the precious metal with the base metal. Preferred examples of the catalyst include a saponite support loaded with about 10-20 wt % cobalt and about 0.1-1 wt % precious metal. The catalyst is optimized for reactions that occur in commercial processes at about 360-400° C., such as in hydrocracking.

摘要翻译： 表现出氢气溢出效应的催化剂涉及显示氢气溢出效应的催化剂的组成和制备催化剂的方法。 该催化剂具有负载在诸如皂石的高多孔载体上的VIB族或VIIIB族的钌基过渡金属，例如钴，镍，钼或钨，贱金属与至少一种贵金属离子交换 第VIIIB组。 该方法包括以下步骤：将贱金属加载到载体上，优选在600℃下用H 2还原贱金属，然后将贵金属与贱金属进行离子交换。 催化剂的优选实例包括负载有约10-20重量％钴和约0.1-1重量％贵金属的皂石载体。 该催化剂针对在约360-400℃的商业过程中发生的反应进行优化，例如在加氢裂化中。