公开(公告)号：US20160272951A1

公开(公告)日：2016-09-22

申请号：US15159931

申请日：2016-05-20

Applicant: BASF Plant Science Company GmbH

Inventor： Toralf Senger ,  Laurent MARTY ,  Sten STYMNE ,  Jenny LINDBERG YILMAZ ,  Johnathan A. NAPIER ,  Olga SAYANOVA ,  Richard HASLAM ,  Ruiz Lopez NOEMI

IPC: C12N9/10 ,  A23K20/158 ,  A23D9/02 ,  C12P7/64 ,  C12N15/82

CPC classification number: C11C1/045 ,  A23D9/02 ,  A23K20/158 ,  A23L33/105 ,  A23L33/115 ,  A23L33/12 ,  A23V2002/00 ,  A23V2250/1862 ,  A23V2250/1868 ,  A23V2250/187 ,  A23V2250/1882 ,  C12N15/8247 ,  C12P7/6427 ,  C12Y203/0102

Abstract: The present invention relates to the recombinant manufacture of polyunsaturated fatty acids. Specifically, it relates to acyltransferase polypeptides, polynucleotides encoding said acyltransferases as well as vectors, host cells, non-human transgenic organisms containing said polynucleotides. Moreover, the present invention contemplates methods for the manufacture of polyunsaturated fatty acids as well as oils obtained by such methods.

Abstract translation: 本发明涉及多不饱和脂肪酸的重组制造。 具体地，本发明涉及酰基转移酶多肽，编码所述酰基转移酶的多核苷酸以及含有所述多核苷酸的载体，宿主细胞，非人转基因生物。 此外，本发明考虑了制备多不饱和脂肪酸的方法以及通过这些方法获得的油。

公开(公告)号：US09139854B2

公开(公告)日：2015-09-22

申请号：US14338673

申请日：2014-07-23

Applicant: BASF Plant Science Company GmbH

Inventor： Toralf Senger ,  Jörg Bauer ,  Johnathan A. Napier

IPC: C12P7/64 ,  C12N9/02 ,  C07H21/02 ,  C12N15/82

CPC classification number: C12P7/6409 ,  C12N9/0071 ,  C12N9/0083 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472 ,  C12P7/6481 ,  C12Y114/19001

Abstract: The present invention relates to polynucleotides from Cochliobolus heterostrophus C5, Cyanothece sp. CCY0110, Mycocentrospora acerin and Hyaloperonospora parasitica, which code for desaturases and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides according to the invention, and to the polypeptides encoded by the polynucleotides. The invention furthermore relates to antibodies against the polypeptides according to the invention. Finally, the invention also relates to production processes for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to their use as drugs, cosmetics, foodstuffs, feedstuffs, preferably fish food, or food supplements.

Abstract translation: 本发明涉及来自嗜球菌（Cocchioiobolus heterostrophus）C5，蓝藻（Cyanothece sp。）的多核苷酸 CCY0110，真菌孢子菌和寄生藻类，代表去饱和酶，可用于重组生产多不饱和脂肪酸。 本发明还涉及包含根据本发明的多核苷酸的载体，宿主细胞和转基因非人生物以及由多核苷酸编码的多肽。 本发明还涉及针对根据本发明的多肽的抗体。 最后，本发明还涉及多不饱和脂肪酸和油，脂和脂肪酸组合物的生产方法以及它们作为药物，化妆品，食品，饲料，优选鱼食或食品添加剂的用途。

公开(公告)号：US20230293615A1

公开(公告)日：2023-09-21

申请号：US18162300

申请日：2023-01-31

Applicant: BASF PLANT SCIENCE COMPANY GMBH ,  Bioriginal Food & Science Corporation

Inventor： Toralf Senger ,  Laurent Marty ,  Irene Kunze ,  Heiko Haertel ,  Steven Craig Bremmer ,  Steven D. Breazeale ,  Joerg Bauer ,  Patricia Vrinten ,  Sten Stymne ,  Jenny Lindberg Yilmaz ,  John A. McElver ,  Dietrich Rein ,  Carl Andre

IPC: A61K36/31 ,  C12Q1/6895

CPC classification number: A61K36/31 ,  C12Q1/6895 ,  C12N15/8247

Abstract: T-DNA for expression of a target gene in a plant, wherein the T-DNA comprises a left and a right border element and at least one expression cassette comprising a promoter, operatively linked thereto a target gene, and downstream thereof a terminator, wherein the length of the T-DNA, measured from left to right border element and comprising the target gene, has a length of at least 30000 bp.

公开(公告)号：US20220184161A1

公开(公告)日：2022-06-16

申请号：US17682694

申请日：2022-02-28

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Toralf Senger ,  Carl Andre

IPC: A61K36/31 ,  C12Q1/6895

Abstract: The present invention generally is concerned with the modification of plant lipids containing PUFAs. In this context, the invention is particularly concerned with plants and plant materials for such modifications, wherein the plants preferably are oilseed plants. Regarding plant parts, the invention is particularly concerned with seeds of such plants and preferably seeds of oilseed plants. The invention is also concerned with plant positions obtainable or obtained by the modification method of the invention, and with full stuff of feedstuff comprising such liquid compositions.

公开(公告)号：US11046937B2

公开(公告)日：2021-06-29

申请号：US16414150

申请日：2019-05-16

Applicant: BASF PLANT SCIENCE COMPANY GMBH ,  Bioriginal Food & Science Corp.

Inventor： Toralf Senger ,  Patricia Vrinten ,  Ze Long Lim

IPC: C12N9/02 ,  C07K14/46 ,  C12P7/64

Abstract: The present invention relates to methods for the conversion of the substrate specificity of desaturases. Specifically, the present invention pertains to a method for the conversion of the substrate specificity of a Δ5 and/or Δ6 desaturase to the substrate specificity of a Δ4 desaturase, the method comprising: identifying regions and/or amino acid residues which control the substrate specificity of (i) the Δ5 and/or Δ6 desaturase and (ii) the Δ4 desaturase; and replacing in the amino acid sequence of the mentioned Δ5 and/or Δ6 desaturase, the regions and/or amino acid residues which control the substrate specificity of the Δ5 and/or Δ6 desaturase, by the corresponding regions and/or amino acid residues which control the substrate specificity of the Δ4 desaturase, thereby converting the substrate specificity of the Δ5 and/or Δ6 desaturase to the substrate specificity of the Δ4 desaturase. The present invention further concerns a method for the conversion of the substrate specificity of a Δ4 desaturase to the substrate specificity of a Δ5 and/or Δ6 desaturase, the method comprising: identifying regions and/or amino acid residues which control the substrate specificity of (i) the Δ4 desaturase and (ii) the Δ5 and/or Δ6 desaturase; and replacing in the amino acid sequence of the indicated Δ4 desaturase, the regions and/or amino acid residues which control the substrate specificity of the Δ4 desaturase, by the corresponding regions and/or amino acid residues which control the substrate specificity of the Δ5 and/or Δ6 desaturase, thereby converting the substrate specificity of the Δ4 desaturase to the substrate specificity of the Δ5 and/or Δ6 desaturase. In addition, the invention encompasses desaturases with converted substrate specificity.

公开(公告)号：US11033593B2

公开(公告)日：2021-06-15

申请号：US15526443

申请日：2015-11-13

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Toralf Senger ,  Laurent Marty ,  Irene Kunze ,  Dietrich Rein ,  Joerg Bauer ,  Carl Andre

IPC: A61K36/31 ,  C12N15/82 ,  C12Q1/6895 ,  C12N9/02 ,  C12N9/00 ,  A61K31/202

Abstract: The present invention provides transgenic Brassica events LBFLFK and LBFDAU and progeny thereof, and cells, seeds, plants comprising DNA diagnostic for these events. The invention also provides artificial oligonucleotide primers and probes that are diagnostic for the LBFLFK and LBFDAU events and their progeny in a sample, and methods for detecting the presence of the LBFLFK and LBFDAU events and their progeny in a sample. The invention further provides oil and commodity products derived from the LBFLFK and LBFDAU events.

公开(公告)号：US09828589B2

公开(公告)日：2017-11-28

申请号：US14936807

申请日：2015-11-10

Applicant: BASF Plant Science Company GmbH

Inventor： Toralf Senger ,  Sten Stymne ,  Jenny Lindberg Yilmaz

IPC: C12N9/10 ,  C12N15/82 ,  A61K8/36 ,  A61K31/202 ,  C12P7/64 ,  A23K20/158 ,  A23L33/115 ,  A23L33/12 ,  A61K8/60 ,  A61Q19/00 ,  A23D9/00 ,  C11B1/00 ,  C11B1/06

CPC classification number: C12N9/1025 ,  A23D9/00 ,  A23K20/158 ,  A23L33/115 ,  A23L33/12 ,  A23V2002/00 ,  A61K8/361 ,  A61K8/606 ,  A61K31/202 ,  A61K2800/10 ,  A61Q19/00 ,  C11B1/00 ,  C11B1/06 ,  C12N9/1029 ,  C12N15/8247 ,  C12P7/6427 ,  C12Y203/00

Abstract: The present invention pertains to the recombinant manufacture of polyunsaturated fatty acids. Specifically, it relates to acyltransferase polypeptides, polynucleotides encoding said acyltransferases as well as vectors, host cells, non-human transgenic organisms containing said polynucleotides. Moreover, the present invention contemplates methods for the manufacture of polyunsaturated fatty acids as well as oils obtained by such methods.

公开(公告)号：US20160168584A1

公开(公告)日：2016-06-16

申请号：US14902715

申请日：2014-07-03

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Toralf Senger ,  Joerg Bauer

IPC: C12N15/82

Abstract: The present invention relates to transgenic nucleic acids, expression cassettes, vectors, plant cells, plant organs and plants. The invention also relates to methods for increasing expression or activity of a target gene, particularly in a plant cell or plant organ, and also to uses of recombinant nucleic acids and expression cassettes to increase expression or activity of a target gene or for manufacturing of a vector, plant cell, plant organ or plant. Incidentally, the invention relates to enhancers for achieving increased expression or activity of a target gene, particularly in a plant cell or plant organ, when operably linked to a promoter functional in such plant cell, plant organ or plant. The invention is described herein with reference to the technical field of production of polyunsaturated fatty acids (PUFAs), without being limited to this technical field.For the production of desired molecules in plant cells, e.g. PUFAs, it is frequently required to express a target gene heterologous to the plant cell, or to overexpress a target gene naturally found in said plant cell.

Abstract translation: 本发明涉及转基因核酸，表达盒，载体，植物细胞，植物器官和植物。 本发明还涉及用于增加靶基因，特别是在植物细胞或植物器官中的表达或活性的方法，以及重组核酸和表达盒的用途以增加靶基因的表达或活性或用于制备 载体，植物细胞，植物器官或植物。 顺便提及，本发明涉及当可操作地连接到在这种植物细胞，植物器官或植物中起作用的启动子时，用于实现靶基因，特别是在植物细胞或植物器官中增加的表达或活性的增强子。 本发明是参照多不饱和脂肪酸（PUFA）的生产技术领域而不受本技术领域的限制。 为了在植物细胞中产生所需的分子，例如。 PUFA通常需要表达与植物细胞异源的靶基因，或过表达在所述植物细胞中天然存在的靶基因。