公开(公告)号：US20160362696A1

公开(公告)日：2016-12-15

申请号：US15116600

申请日：2015-02-06

Applicant: BASF SE

Inventor： Joanna Martyna Krawczyk ,  Stefan Haefner ,  Hartwig Schröder ,  Esther Dantas Costa ,  Oskar Zelder ,  Gregory Von Abendroth ,  Christoph Wittmann ,  René Stellmacher ,  Judith Becker

IPC: C12N15/52 ,  C12N15/74 ,  C12N9/04 ,  C12N9/10 ,  C12P7/46 ,  C12N9/12

CPC classification number: C12N15/52 ,  C12N1/02 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1288 ,  C12N15/74 ,  C12P7/46 ,  C12Y101/01 ,  C12Y203/01054 ,  C12Y207/08031

Abstract: The present invention relates to a modified microorganism having, compared to its wild-type, a reduced activity of the enzyme that is encoded by the wcaJ-gene. The present invention also relates to a method for producing an organic compound and to the use of a modified microorganism.

Abstract translation: 本发明涉及与其野生型相比具有由wcaJ基因编码的酶的活性降低的修饰微生物。 本发明还涉及有机化合物的制造方法和改性微生物的用途。

公开(公告)号：US20160348082A1

公开(公告)日：2016-12-01

申请号：US15116669

申请日：2015-02-02

Applicant: BASF SE

Inventor： Joanna Martyna Krawczyk ,  Stefan Haefner ,  Hartwig Schröder ,  Esther Dantas Costa ,  Oakar Zelder ,  Gregory von Abendroth ,  Christoph Wittmann ,  René Stellmacher ,  Judith Becker ,  Anna Lange ,  Benjamin J. Lyons ,  Thomas J. Lyons ,  Eudes De Crecy ,  Ewa Hughes

IPC: C12N9/12 ,  C12P7/46

CPC classification number: C12N9/1205 ,  C12N15/52 ,  C12P7/46 ,  C12Y207/0104

Abstract: The present invention relates to a modified microorganism having, compared to its wild-type, a reduced activity of the enzyme that is encoded by the pykA-gene. The present invention also relates to a method for producing an organic compound and to the use of modified microorganisms.

Abstract translation: 本发明涉及与其野生型相比具有由pykA基因编码的酶的活性降低的修饰微生物。 本发明还涉及一种生产有机化合物的方法和使用改性微生物。

公开(公告)号：US20150197776A1

公开(公告)日：2015-07-16

申请号：US14676317

申请日：2015-04-01

Applicant: BASF SE

Inventor： Hartwig Schröder ,  Stefan Haefner ,  Gregory Von Abendroth ,  Rajan Hollmann ,  Aline Raddatz ,  Hansgeorg Ernst ,  Hans Gurski

IPC: C12P7/46

CPC classification number: C12P7/46 ,  C12N1/32 ,  C12N9/1029 ,  C12R1/01 ,  C12Y203/01054

Abstract: The present invention relates to bacterial strains, capable of utilizing glycerol as a carbon source for the fermentative production of succinic acid, wherein said strains are genetically modified so that they comprise a deregulation of their endogenous pyruvate-formate-lyase enzyme activity, as well as to methods of producing organic acids, in particular succinic acid, by making use of such microorganism. The present invention also relates to the downstream processing of the produced organic acids by cation exchange chromatography.

Abstract translation: 本发明涉及能够利用甘油作为发酵生产琥珀酸的碳源的细菌菌株，其中所述菌株被遗传修饰，使得它们包含其内源性丙酮酸 - 甲酸裂解酶活性的失调，以及 涉及通过利用这种微生物生产有机酸，特别是琥珀酸的方法。 本发明还涉及通过阳离子交换层析对所生产的有机酸的下游加工。

公开(公告)号：US09023632B2

公开(公告)日：2015-05-05

申请号：US14150206

申请日：2014-01-08

Applicant: BASF SE

Inventor： Hartwig Schröder ,  Stefan Haefner ,  Gregory Von Abendroth ,  Rajan Hollmann ,  Aline Raddatz ,  Hansgeorg Ernst ,  Hans Gurski

IPC: C12P7/46 ,  C12P7/44 ,  C12N1/32 ,  C12N9/10 ,  C12R1/01

CPC classification number: C12P7/46 ,  C12N1/32 ,  C12N9/1029 ,  C12R1/01 ,  C12Y203/01054

Abstract: The present invention relates to bacterial strains, capable of utilizing glycerol as a carbon source for the fermentative production of succinic acid, wherein said strains are genetically modified so that they comprise a deregulation of their endogenous pyruvate-formate-lyase enzyme activity, as well as to methods of producing organic acids, in particular succinic acid, by making use of such microorganism. The present invention also relates to the downstream processing of the produced organic acids by cation exchange chromatography.

Abstract translation: 本发明涉及能够利用甘油作为发酵生产琥珀酸的碳源的细菌菌株，其中所述菌株被遗传修饰，使得它们包含其内源性丙酮酸 - 甲酸裂解酶活性的失调，以及 涉及通过利用这种微生物生产有机酸，特别是琥珀酸的方法。 本发明还涉及通过阳离子交换层析对所生产的有机酸的下游加工。

公开(公告)号：US20140011243A1

公开(公告)日：2014-01-09

申请号：US13935043

申请日：2013-07-03

Applicant: Wintershall Holding GmbH ,  BASF SE

Inventor： Beata Brockmann ,  Andrea Herold ,  Oskar Zelder ,  Stefan Haefner ,  Christian Fleck ,  Hartwig Schröder ,  Mari Granström ,  Julia Kristiane Schmidt

IPC: C12P19/04 ,  C12N15/80

CPC classification number: C12P19/04 ,  C08B37/0024 ,  C08L5/00 ,  C12N9/1051 ,  C12N15/80 ,  C12P19/18 ,  C12Y204/01034 ,  Y02P20/52

Abstract: The present invention relates to genetically modified microorganisms capable of producing beta-glucans, characterized in that said genetically modified microorganism overexpresses (i) a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity, and/or (ii) a polypeptide having 1,3-β-D-glucan synthase-activity, compared to a corresponding non-modified control microorganism of the same strain. The present invention also relates to the use of a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity or the use of such a polypeptide for producing β-glucans. Furthermore, the present invention relates to methods for producing β-glucans comprising the introduction of a promoter upstream of a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity thereby increasing the expression of said polynucleotide, or a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity into a microorganism being able to synthesize β-glucans.

Abstract translation: 本发明涉及能够产生β-葡聚糖的转基因微生物，其特征在于所述遗传修饰的微生物过表达（i）编码具有1,3-β-D-葡聚糖合酶活性的多肽的多核苷酸和/或（ii ）具有1,3-β-D-葡聚糖合酶活性的多肽，与相同菌株的相应未修饰的对照微生物相比较。 本发明还涉及编码具有1,3-β-D-葡聚糖合酶活性的多肽的多核苷酸的用途或这种多肽用于产生β-葡聚糖的用途。 此外，本发明涉及生产β-葡聚糖的方法，包括在编码具有1,3-β-D-葡聚糖合酶活性的多肽的多核苷酸上游引入启动子，从而增加所述多核苷酸的表达，或多核苷酸 将具有1,3-β-D-葡聚糖合酶活性的多肽编码成能够合成β-葡聚糖的微生物。