公开(公告)号：US10533201B2

公开(公告)日：2020-01-14

申请号：US15509993

申请日：2015-06-30

Applicant: BASF SE

Inventor： Vaidotas Navickas ,  Kai-Uwe Baldenius ,  Michael Breuer ,  Patricia Wildberger ,  Martin Pfeiffer ,  Bernd Nidetzky

IPC: C07H11/04 ,  C12P19/02 ,  C12P19/26 ,  C12N9/10 ,  C12N9/16 ,  C07H1/02

Abstract: The present invention relates to methods of producing sugar phosphates by enzymatic transphosphorylation, wherein the phosphoryl transfer from phosphate donor substrates to sugar substrates is catalyzed by an enzyme having alpha-glucose-1-phosphatase activity. Furthermore, the present invention relates to a biocatalyst having sucrose phosphorylase as well as alpha-glucose-1-phosphatase activity and its use. The production of an enzyme having alpha-glucose-1-phosphatase activity and its use in the transphosphorylation of sugar substrates is also presented herein.

公开(公告)号：US10428361B2

公开(公告)日：2019-10-01

申请号：US15559962

申请日：2016-02-19

Applicant: BASF SE

Inventor： Vaidotas Navickas ,  Michael Breuer ,  Michael Puhl ,  Melanie Weingarten ,  Kai-Uwe Baldenius ,  Wolfgang Siegel

IPC: C12P19/02 ,  C12N9/04 ,  C12N9/08

Abstract: The present invention relates to biocatalytic methods, comprising purely enzymatic, mixed enzymatic-fermentative and purely fermentative methods, for the direct single-step conversion of L-fucitol to L-fucose, in order to easily obtain L-fucose at high amounts and levels of purity. Suitable recombinant microorganisms and fungi are further disclosed and also the use thereof in said method for the single-step conversion to L-fucose.

公开(公告)号：US10190112B2

公开(公告)日：2019-01-29

申请号：US15234529

申请日：2016-08-11

Applicant: BASF SE

Inventor： Michael Breuer ,  Bernhard Hauer ,  Dieter Jendrossek ,  Gabriele Siedenburg ,  Juergen Pleiss ,  Demet Sirim ,  Silvia Fadenrecht

IPC: C12N9/88 ,  C12N9/10 ,  C12N15/00 ,  C12P7/02 ,  C12P5/00 ,  C12N9/90 ,  C12P7/22

Abstract: The present invention relates to novel mutants with cyclase activity and use thereof in a method for biocatalytic cyclization of terpenes, such as in particular for the production of isopulegol by cyclization of citronellal; a method for the preparation of menthol and methods for the biocatalytic conversion of further compounds with structural motifs similar to terpene.

公开(公告)号：US20160340666A1

公开(公告)日：2016-11-24

申请号：US15234529

申请日：2016-08-11

Applicant: BASF SE

Inventor： Michael Breuer ,  Bernhard Hauer ,  Dieter Jendrossek ,  Gabrielle Siedenburg ,  Juergen Pleiss ,  Demet Sirim ,  Silivia Fadenrecht

IPC: C12N9/88 ,  C12P7/22 ,  C12P5/00

CPC classification number: C12N9/88 ,  C12N9/90 ,  C12P5/007 ,  C12P7/02 ,  C12P7/22 ,  C12Y406/01 ,  C12Y504/99017 ,  Y02P20/52

Abstract: The present invention relates to novel mutants with cyclase activity and use thereof in a method for biocatalytic cyclization of terpenes, such as in particular for the production of isopulegol by cyclization of citronellal; a method for the preparation of menthol and methods for the biocatalytic conversion of further compounds with structural motifs similar to terpene.

公开(公告)号：US20160168597A1

公开(公告)日：2016-06-16

申请号：US14908656

申请日：2014-07-21

Applicant: BASF SE

Inventor： Michael Breuer ,  Boris Breitscheidel ,  Hans-Günter Wagner ,  Detlef Kratz ,  Bernhard Hauer ,  Daniel Scheps ,  Bernd Nebel

IPC: C12P7/16

CPC classification number: C12P7/16 ,  C12Y114/15003 ,  C12Y118/01001 ,  Y02E50/10

Abstract: A process for preparing 1-butanol from butane by incubating a host organism having a functional P153 enzyme under elevated butane pressure in the presence of oxygen.

Abstract translation: 通过在氧气存在下在升高的丁烷压下温育具有功能性P153酶的宿主生物体从丁烷制备1-丁醇的方法。