公开(公告)号：US20170356039A1

公开(公告)日：2017-12-14

申请号：US15510890

申请日：2014-11-21

Applicant: BGI SHENZHEN CO., LIMITED

Inventor： Yuan Jiang ,  Jing Guo ,  Xiaojun Ji ,  Chunyu Geng ,  Kai Tian ,  Xia Zhao ,  Huaiqian Xu ,  Wenwei Zhang ,  Hui Jiang ,  Radoje Drmanac

IPC: C12Q1/68 ,  C12N15/10

Abstract: Provided are a vesicular linker and a single-chain cyclic library constructed by using the linker. The library can be used for RNA sequencing and other sequencing platforms dependent on a single-stranded cyclic library, and has the advantages of high throughput sequencing, high accuracy and simple operations.

公开(公告)号：US20250136974A1

公开(公告)日：2025-05-01

申请号：US18725483

申请日：2021-12-31

Applicant: BGI Shenzhen

Inventor： Ou Wang ,  Xiao Shi ,  Xun Xu ,  Wenwei Zhang ,  Fei Guo ,  Siyu Bao ,  Yuliang Dong ,  Tao Zeng

IPC: C12N15/11 ,  C12N15/10 ,  C12Q1/6869

Abstract: The present invention relates to a nucleic acid molecule capable of blocking motor protein, a library containing the nucleic acid molecule, a method for constructing the nucleic acid molecule or library containing the same, and an application of the nucleic acid molecule or library containing same. The nucleic acid molecule contains at the end a modified nucleotide capable of blocking a motor protein, and the modified nucleotide is as shown in Formula I or Formula I′:

公开(公告)号：US20250066747A1

公开(公告)日：2025-02-27

申请号：US18723588

申请日：2021-12-31

Applicant: BGI Shenzhen

Inventor： Xun Xu ,  Fei Guo ,  Qiaoxia Hu ,  Denghui Li ,  Junyi Chen ,  Lele Wang ,  Ziyu Zhao ,  Zhouxiang Ji ,  Tao Zeng ,  Ou Wang ,  Yuxiang Li ,  Yuliang Dong ,  Wenwei Zhang

IPC: C12N9/14 ,  C12Q1/6869

Abstract: Provided is helicase BCH1X, which comprises an amino acid sequence represented by SEQ ID NO: 1 or 2. Further provided are a complex structure comprising helicase BCH1X and a binding moiety used for binding to a polynucleotide, and a use thereof in the control and characterization of a polynucleotide and in single molecule nanopore sequencing.

公开(公告)号：US20240392265A1

公开(公告)日：2024-11-28

申请号：US18564400

申请日：2021-09-08

Applicant: BGI SHENZHEN

Inventor： Lili Zhai ,  Qingqing Xie ,  Ruyin Cao ,  Yue Zheng ,  Yuliang Dong ,  Wenwei Zhang ,  Xun Xu ,  Zhuo Chen

IPC: C12N9/12 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: Provided are a thermostable B-family DNA polymerase mutant and an application thereof. The thermostable B-family DNA polymerase mutant is provided to mutate amino acid residues in at least two of three sites of group A, i.e., 408, 409, and 410 in an amino acid sequence of thermostable B-family DNA polymerases to obtain proteins having DNA polymerase activity. A-group sites (408/409/410), B-group sites (451/485), C-group sites (389/383/384), D-group sites (589/67/680), E-group sites (491/493/494/497), and F-group sites (474/478/486/480/484) of several B-family DNA polymerases are analyzed to design the mutant.

公开(公告)号：US10563196B2

公开(公告)日：2020-02-18

申请号：US15519530

申请日：2014-10-17

Applicant: BGI SHENZHEN

Inventor： Chunyu Geng ,  Hongyan Han ,  Guangying Guo ,  Wenwei Zhang ,  Hui Jiang ,  Yuan Jiang

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/68 ,  C12Q1/6853 ,  C12Q1/686 ,  C40B40/08 ,  C40B50/06

Abstract: The present invention provides a primer for nucleic acid random fragmentation and a nucleic acid random fragmentation method. The primer consists of a plurality of upstream random primers and downstream random primers. The sequence composition of the upstream random primers is 5′-X-Y-3′, and the sequence composition of the downstream random primers is 5′-P-Y′-X′-close-3′, wherein Y and Y′ are random sequences, X is all or part of sequences of a sequencing platform 5′ end adaptor, X′ is all or part of sequences of a sequencing platform 3′ end adaptor, P is phosphorylation modification, and close is close modification. The primer of the present invention adopts double random anchoring of both the upstream random primers and the downstream random primers, and a DNA sample can be randomly broken.

公开(公告)号：US20180030532A1

公开(公告)日：2018-02-01

申请号：US15528362

申请日：2014-11-21

Applicant: BGI Shenzhen Co., Limited

Inventor： Yuan Jiang ,  Xia Zhao ,  Qiaoling Li ,  Shengmao Liu ,  Bo Wang ,  Li Chen ,  Wenwei Zhang ,  Hui Jiang ,  Radoje Drmanac

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/68 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q2525/191 ,  C12Q2525/301 ,  C12Q2535/122

Abstract: Provided are an adaptor element in a bubble shape, a method of constructing a sequencing library with such an adapter element. The adaptor element is a hybrid formed with a long-chain nucleic acid A and a short-chain nucleic acid B. The hybrid is in the bubble shape with paired regions at two terminals and a non-paired region in the middle.