摘要:
An isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the rpsL gene is present in enhanced form, as well as the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
摘要翻译:一种分离的多核苷酸,其包含选自以下的多核苷酸序列:a)与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸的程度相同的多核苷酸,b) 编码多肽的多核苷酸,其包含与SEQ ID No.2的氨基酸序列至少70%相同的氨基酸序列,c)与a)或b)的多核苷酸互补的多核苷酸, 和d)包含a),b)或c)的多核苷酸序列的至少15个连续核苷酸的多核苷酸,以及使用棒状细菌发酵制备L-氨基酸的方法,其中至少存在rpsL基因 增强形式,以及使用包含根据本发明的序列的多核苷酸作为杂交探针。
摘要:
The present invention is directed to nucleotide sequences coding for a bacterial enolase enzyme. These sequences may be used in improved methods for the fermentative preparation of amino acids using coryneform bacteria.
摘要:
The present invention relates to polynucleotides corresponding to the rpoB gene and which encode the &bgr;-subunit of RNA polymerase B, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having activity of the &bgr;-subunit of RNA polymerase B.
摘要翻译:本发明涉及对应于rpoB基因并编码RNA聚合酶B的β-亚单位的多核苷酸,产生L-氨基酸的方法,以及筛选编码具有RNA聚合酶的β-亚基活性的蛋白质的多核苷酸的方法 B.
摘要:
The invention related to an isolated nucleic acid consisting of a fragment of the polynucleotide of Corynebacterium glutamicum citA gene or a fragment of the full complement of the polynucleotide of the C. glutamicum citA gene wherein said fragment is at least 30 consecutive nucleotide in length, and the vector and host cell harboring these nucleic acids.
摘要:
The present invention relates to polynucleotides corresponding to the lysR1 gene and which encode a LysR1 transcriptional regulator, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having LysR1 transcriptional regulator activity.
摘要:
The present invention relates to polynucleotides corresponding to the lysR1 gene and which encode a LysR1 transcriptional regulator, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having LysR3 transcriptional regulator activity.
摘要:
Isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c),and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the lysR2 gene is present in attenuated form, and the use of the polynucleotide sequences as hybridization probes.
摘要翻译:分离的多核苷酸,其包含选自以下的多核苷酸序列:a)与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸的程度相同的多核苷酸,b)多核苷酸, 其编码多肽,其包含与SEQ ID No.2的氨基酸序列至少70%的程度相同的氨基酸序列,c)与a)或b)的多核苷酸互补的多核苷酸, 和d)包含a),b)或c)的多核苷酸序列的至少15个连续核苷酸的多核苷酸,以及使用棒状细菌发酵制备L-氨基酸的方法,其中至少lysR2基因存在于减毒 形式,以及使用多核苷酸序列作为杂交探针。
摘要:
The invention relates to polynucleotides corresponding to the ccpA2 gene and which encode a CcpA2 catabolite control protein, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having CcpA2 catabolite control activity.
摘要:
The invention relates to a process for the preparation of L-amino acids, especially L-threonine, in which the following steps are carried out: a) fermentation of microorganisms of the family Enterobacteriaceae which produce the desired L-amino acid and in which the mglB gene, or nucleotide sequences coding therefor, is (are) enhanced and, in particular, overexpressed, b) enrichment of the desired L-amino acid in the medium or in the cells of the bacteria, and c) isolation of the desired L-amino acid.
摘要:
The invention relates to isolated polynucleotides comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the citA gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
摘要翻译:本发明涉及分离的多核苷酸,其包含多核苷酸序列,所述多核苷酸序列选自a)多核苷酸,其与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸的程度相同至少70% b)编码多肽的多核苷酸,其包含与SEQ ID No.2的氨基酸序列至少70%相同的氨基酸序列,c)与a)的多核苷酸互补的多核苷酸, 或b),和d)包含a),b)或c)的多核苷酸序列的至少15个连续核苷酸的多核苷酸,以及使用棒状细菌发酵制备L-氨基酸的方法,其中至少有citA基因 以减毒形式存在,并且使用包含根据本发明的序列的多核苷酸作为杂交探针。