公开(公告)号：US20150064718A1

公开(公告)日：2015-03-05

申请号：US14471060

申请日：2014-08-28

Applicant: Corning Incorporated

Inventor： Stephen Joseph Caracci ,  Martin Joseph Murtagh ,  Christopher Bankole Shogbon ,  Yue Zhou

IPC: G01N33/543 ,  B29C47/00 ,  B29C47/40 ,  H01F1/01

CPC classification number: G01N33/5434 ,  B29B7/46 ,  B29B7/726 ,  B29B9/06 ,  B29C48/001 ,  B29C48/0022 ,  B29C48/022 ,  B29C48/04 ,  B29C48/40 ,  B29C48/402 ,  B29C2793/009 ,  B29K2023/14 ,  B29K2025/08 ,  B29K2035/00 ,  B29K2105/0085 ,  B29K2105/16 ,  B29K2503/06 ,  B29K2505/00 ,  B29K2505/12 ,  B29K2995/0008 ,  B29K2995/007 ,  B29K2995/0077 ,  B29K2995/0094 ,  C08K3/22 ,  C08K2003/2265 ,  C08K2003/2275 ,  C08K2201/003 ,  H01F1/01 ,  Y10T428/2982 ,  C08L51/06 ,  C08L25/08

Abstract: An organic/inorganic composite is provided. The organic/inorganic composite materials are sufficiently hard and brittle to be ground to form particles suitable for biological or chemical separation applications. The organic/inorganic composite materials can be magnetic or magnetically susceptible and can have functional reactive groups to allow attachment of biomolecules. Methods of making and using the organic/inorganic composites are also provided.

Abstract translation: 提供有机/无机复合材料。 有机/无机复合材料足够硬且易碎以研磨以形成适于生物或化学分离应用的颗粒。 有机/无机复合材料可以是磁性或磁性敏感的，并且可以具有功能反应性基团以允许附着生物分子。 还提供了制备和使用有机/无机复合材料的方法。

公开(公告)号：US20150010999A1

公开(公告)日：2015-01-08

申请号：US14375614

申请日：2013-01-31

Applicant: CORNING INCORPORATED

Inventor： Stephen Joseph Caracci ,  David Henry ,  Jessica Jo Kelley ,  Mark Alan Lewis ,  Yue Zhou

IPC: C12N5/00 ,  C12N5/0735 ,  B05D3/02 ,  C12N5/0775

CPC classification number: C12N5/0068 ,  B05D3/0254 ,  C12M23/20 ,  C12N5/0037 ,  C12N5/0056 ,  C12N5/0606 ,  C12N5/0663 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2533/30 ,  C12N2533/40 ,  C12N2533/50

Abstract: An aqueous cell culture medium composition includes an aqueous cell culture solution configured to support the culture of mammalian cells. The composition further includes a synthetic polymer conjugated to a polypeptide dissolved in the aqueous cell culture solution. The synthetic polymer conjugated to a polypeptide is configured to attach to the surface of a cell culture article under cell culture conditions. Incubation of the aqueous cell culture medium composition on a cell culture surface under cell culture conditions results is attachment to the surface of the synthetic polymer conjugated to the polypeptide.

Abstract translation: 水性细胞培养基组合物包括配置成支持哺乳动物细胞培养的水性细胞培养液。 该组合物还包括与溶解在水性细胞培养溶液中的多肽缀合的合成聚合物。 与多肽缀合的合成聚合物被配置为在细胞培养条件下附着于细胞培养物品的表面。 水细胞培养基组合物在细胞培养条件下在细胞培养物表面上的孵育结果是与多肽缀合的合成聚合物表面的附着。

公开(公告)号：US12173311B2

公开(公告)日：2024-12-24

申请号：US18226559

申请日：2023-07-26

Applicant: CORNING INCORPORATED

Inventor： Ye Fang ,  Ann MeeJin Ferrie ,  Vasiliy Nikolaevich Goral ,  David Henry ,  Martial Hervy ,  Corinne Walerack ,  Yue Zhou

IPC: C12N5/00 ,  C08J3/24 ,  C09D167/00

Abstract: A cell culture article includes a substrate having a polymer coating that is conducive to colony passaging of cells cultured on the coating. Example polymer coatings are formed from polygalacturonic acid (PGA), alginate, or combinations thereof. Cells cultured on the polymer coating can be separated from the substrate as a colony or layer of cells by exposing the polymer coating to (i) a chelating agent, (ii) a proteinase-free enzyme, or (iii) a chelating agent and a proteinase-free enzyme.

公开(公告)号：US20240344008A1

公开(公告)日：2024-10-17

申请号：US18293618

申请日：2022-07-21

Applicant: CORNING INCORPORATED

Inventor： Yujian Sun ,  Yue Zhou

IPC: C12M1/12 ,  C12M1/00

CPC classification number: C12M25/18 ,  C12M29/06

Abstract: A packed-bed bioreactor system for culturing cells is provided. The system includes a cell culture vessel having at least one interior reservoir, an inlet fluidly connected to the reservoir, and an outlet fluidly connected to the reservoir; and a cell culture matrix disposed in the reservoir and arranged as a fixed bed. The cell culture matrix includes a substrate configured for adhering cells thereto. The cell culture matrix maintains a uniform flow within the reservoir throughout the cell culture matrix.

公开(公告)号：US11746322B2

公开(公告)日：2023-09-05

申请号：US17022764

申请日：2020-09-16

Applicant: CORNING INCORPORATED

Inventor： Ye Fang ,  Ann MeeJin Ferrie ,  Vasiliy Nikolaevich Goral ,  David Henry ,  Martial Hervy ,  Corinne Walerack ,  Yue Zhou

IPC: C12N5/00 ,  C08J3/24 ,  C09D167/00

CPC classification number: C12N5/0075 ,  C08J3/24 ,  C09D167/00 ,  C12N5/0068 ,  C12N2533/32 ,  C12N2533/40 ,  C12N2533/50 ,  C12N2533/74 ,  C12N2537/10

Abstract: A cell culture article includes a substrate having a polymer coating that is conducive to colony passaging of cells cultured on the coating. Example polymer coatings are formed from polygalacturonic acid (PGA), alginate, or combinations thereof. Cells cultured on the polymer coating can be separated from the substrate as a colony or layer of cells by exposing the polymer coating to (i) a chelating agent, (ii) a proteinase-free enzyme, or (iii) a chelating agent and a proteinase-free enzyme.

公开(公告)号：US20210207098A1

公开(公告)日：2021-07-08

申请号：US17126348

申请日：2020-12-18

Applicant: CORNING INCORPORATED

Inventor： Paula Jean Dolley-Sonneville ,  David Henry ,  Jeffery Joseph Scibek ,  Yue Zhou

IPC: C12N5/0775 ,  C12N5/00

Abstract: A method of making a cell culture article is provided. The method includes forming a microcarrier from a microcarrier composition comprising a polygalacturonic acid compound or an alginic acid compound, infiltrating the microcarrier with a drying formulation to form an infiltrated microcarrier, and drying the infiltrated microcarrier to form a dried microcarrier, wherein the drying formulation comprises at least one of a saccharide and a monovalent cation.

公开(公告)号：US20180051247A1

公开(公告)日：2018-02-22

申请号：US15537111

申请日：2015-12-16

Applicant: Corning Incorporated

Inventor： Ye Fang ,  Ann MeeJin Ferrie ,  Vasiliy Nikoleavich Goral ,  David Henry ,  Martial Hervy ,  Corinne walerack ,  Yue Zhou

IPC: C12N5/00 ,  C08J3/24 ,  C09D167/00

CPC classification number: C12N5/0075 ,  C08J3/24 ,  C09D167/00 ,  C12N5/0068 ,  C12N2533/32 ,  C12N2533/40 ,  C12N2533/50 ,  C12N2533/74 ,  C12N2537/10

Abstract: A cell culture article includes a substrate having a polymer coating that is conducive to colony passaging of cells cultured on the coating. Example polymer coatings are formed from polygalacturonic acid (PGA), alginate, or combinations thereof. Cells cultured on the polymer coating can be separated from the substrate as a colony or layer of cells by exposing the polymer coating to (i) a chelating agent, (ii) a proteinase-free enzyme, or (iii) a chelating agent and a proteinase-free enzyme.