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    METHODS FOR MODULATING TAL SPECIFICITY
    22.
    发明申请
    METHODS FOR MODULATING TAL SPECIFICITY 审中-公开
    调节特异性的方法

    公开(公告)号：US20150218230A1

    公开(公告)日：2015-08-06

    申请号：US14424757

    申请日：2013-08-30

    Applicant: CELLECTIS, S.A.

    Inventor： Alexandre Juillerat ,  Philippe Duchateau

    IPC: C07K14/195 ,  C07H21/00

    CPC classification number: C07K14/195 ,  C07H21/00 ,  C07K2319/80 ,  C12N15/10 ,  C12N15/102 ,  C12N15/63 ,  C12N15/8216 ,  C12N2501/60

    Abstract: Methods for improving or modulating targeting specificity of TALE proteins by introducing alternative RVDs into their modular nucleic acid binding domains. Polynucleotides encoding TALE proteins having alternative targeting specificity towards a nucleic acid target sequence. TALE proteins having alternative targeting specificity towards a nucleic acid target sequence and methods of making and using them.

    Abstract translation: 通过将替代RVD引入其模块化核酸结合结构域来改善或调节TALE蛋白的靶向特异性的方法。 编码具有针对核酸靶序列的替代靶向特异性的TALE蛋白的多核苷酸。 具有针对核酸靶序列的替代靶向特异性的TALE蛋白质及其制备和使用它们的方法。

    Transcription Activator-Like Effector (TALE) Fusion Protein
    23.
    发明申请
    Transcription Activator-Like Effector (TALE) Fusion Protein 审中-公开
    转录激活子样效应物（TALE）融合蛋白

    公开(公告)号：US20150203871A1

    公开(公告)日：2015-07-23

    申请号：US14405283

    申请日：2013-06-05

    Applicant: Cellectis

    Inventor： Alexandre Juillerat ,  Philippe Duchateau

    IPC: C12N15/90

    CPC classification number: C12N15/907 ,  C07K2319/80 ,  C12N9/22 ,  C12N15/10

    Abstract: The present invention relates to Transcription Activator-Like Effector (TALE) derived proteins that allow to efficiently target and/or process double stranded nucleic acid sequences. The proteins of the invention are typically chimeric protein monomers composed of a core scaffold comprising Repeat Variable Dipeptide regions (RVDs) having binding specificity to a DNA target sequence, to which is fused a catalytic domain to its N-terminal. This later catalytic domain, which can be a monomer of a nuclease, is placed at this position to possibly interact with another catalytic domain fused to another TAL monomer, such that, when said monomers are binding to their respective target DNA sequences, both catalytic domains form a catalytic entity likely to process DNA in the proximity of these target sequences. This new TAL architecture makes it possible to target only one DNA strand, which is not the case, for instance, with classical TALEN architectures. The present invention also relates to vectors encoding such proteins and compositions or kits in which Transcription Activator-Like Effector (TALE) proteins of the present invention are used.

    Abstract translation: 本发明涉及允许有效靶向和/或加工双链核酸序列的转录激活子样效应子（TALE）衍生蛋白。 本发明的蛋白质通常是由包含对DNA靶序列具有结合特异性的重复可变二肽区域（RVD）的核心支架构成的嵌合蛋白质单体，将催化结构域融合到其N-末端。 该后续的催化结构域（其可以是核酸酶的单体）置于该位置以可能与另一个与另一个TAL单体融合的另一催化结构域相互作用，使得当所述单体与其各自的靶DNA序列结合时，两个催化结构域 形成可能在这些靶序列附近处理DNA的催化实体。 这种新的TAL架构使得可以仅靶向一条DNA链，而不是例如经典的TALEN架构。 本发明还涉及编码这样的蛋白质的载体，其中使用本发明的转录激活剂样效应子（TALE）蛋白质的组合物或试剂盒。

    Transcription activator-like effector (TALE) fusion protein
    28.
    发明授权
    Transcription activator-like effector (TALE) fusion protein 有权

    公开(公告)号：US10815500B2

    公开(公告)日：2020-10-27

    申请号：US14405283

    申请日：2013-06-05

    Applicant: Alexandre Juillerat ,  Philippe Duchateau ,  Cellectis

    Inventor： Alexandre Juillerat ,  Philippe Duchateau

    IPC: C12N15/90 ,  C12N9/22 ,  C12N15/10

    Abstract: The present invention relates to Transcription Activator-Like Effector (TALE) derived proteins that allow efficient targeting and/or processing of double stranded nucleic acid sequences. The proteins of the invention are typically chimeric protein monomers composed of a core scaffold comprising Repeat Variable Dipeptide regions (RVDs) having binding specificity to a DNA target sequence, to which is fused a catalytic domain to its N-terminus. This later catalytic domain, which can be a monomer of a nuclease, is placed at this position to possibly interact with another catalytic domain fused to another TAL monomer, such that, when the monomers are binding to their respective target DNA sequences, both catalytic domains form a catalytic entity likely to process DNA in the proximity of these target sequences. This new TAL architecture makes it possible to target only one DNA strand, which is not the case, for instance, with classical TALEN architectures. The present invention also relates to vectors encoding such proteins and compositions or kits in which Transcription Activator-Like Effector (TALE) proteins of the present invention are used.

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