公开(公告)号：US10301677B2

公开(公告)日：2019-05-28

申请号：US15603239

申请日：2017-05-23

Applicant: Cellular Research, Inc.

Inventor： Eleen Shum ,  Glenn Fu ,  Craig Betts

IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6806 ,  G06F19/20 ,  C12N15/10

Abstract: This disclosure provides methods and compositions for removing one or more high abundance species from a plurality of nucleic acid molecules. In some embodiments, the methods and compositions can be used for normalizing nucleic acid libraries. In some embodiments, molecular labels are used in conjunction with the methods and compositions disclosed herein to improve sequencing efficiency.

公开(公告)号：US20180327866A1

公开(公告)日：2018-11-15

申请号：US16038887

申请日：2018-07-18

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P.A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/6888 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6874

CPC classification number: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2563/159 ,  C12Q2563/185

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US20180073073A1

公开(公告)日：2018-03-15

申请号：US15557789

申请日：2016-03-16

Applicant: Cellular Research, Inc.

Inventor： Glenn Fu ,  Stephen P.A. Fodor

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6869 ,  C12Q2525/161 ,  C12Q2525/179 ,  C12Q2537/16 ,  C12Q2545/114 ,  C12Q2563/159 ,  C12Q2563/179

Abstract: Disclosed herein are methods for estimating the copy number of a target chromosome in a sample. In some embodiments, the methods include: partitioning a sample comprising one or more copies of the first target chromosome, into a plurality of partitioned samples; stochastically barcoding the one or more copies of the first target chromosome in the plurality of partitioned samples using a first plurality of stochastic barcodes, wherein each of the first plurality of stochastic barcodes comprises a first chromosome label and a first molecular label; and estimating the copy number of the first target chromosome in the sample using the first chromosome label and the second molecular label. The disclosed methods can be used for haplotype phasing, aneuploidy determination, and DNA sequencing.

公开(公告)号：US20170342484A1

公开(公告)日：2017-11-30

申请号：US15603239

申请日：2017-05-23

Applicant: Cellular Research, Inc.

Inventor： Eleen Shum ,  Glenn Fu ,  Craig Betts

IPC: C12Q1/68 ,  C12N15/10 ,  G06F19/20

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12N15/1093 ,  C12Q1/6806 ,  G06F19/20 ,  C12Q2537/159 ,  C12Q2563/179

Abstract: This disclosure provides methods and compositions for removing one or more high abundance species from a plurality of nucleic acid molecules. In some embodiments, the methods and compositions can be used for normalizing nucleic acid libraries. In some embodiments, molecular labels are used in conjunction with the methods and compositions disclosed herein to improve sequencing efficiency.

公开(公告)号：US20170154421A1

公开(公告)日：2017-06-01

申请号：US15409355

申请日：2017-01-18

Applicant: Cellular Research, Inc.

Inventor： Glenn Fu ,  Roger Rudoff ,  David Stern ,  George T. Wu ,  Stephen P.A. Fodor ,  Geoffrey Facer

IPC: G06T7/00 ,  G01N21/64

CPC classification number: G06T7/0012 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2021/6439 ,  G01N2201/129 ,  G06K9/6267 ,  G06T7/277 ,  G06T2207/10056 ,  G06T2207/10064 ,  G06T2207/30072 ,  G06T2207/30242

Abstract: Methods, systems and platforms for digital imaging of multiple regions of an array, and detection and counting of the labeled features thereon, are described.

公开(公告)号：US09567646B2

公开(公告)日：2017-02-14

申请号：US14800526

申请日：2015-07-15

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P. A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68

CPC classification number: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2563/159 ,  C12Q2563/185

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US20150299784A1

公开(公告)日：2015-10-22

申请号：US14472363

申请日：2014-08-28

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P.A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68

CPC classification number: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2563/159 ,  C12Q2563/185

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

Abstract translation: 本公开提供了用于单个细胞的多重核酸分析的方法，组合物和试剂盒。 方法，组合物和系统可用于大规模并行单细胞测序。 方法，组成和系统可用于同时分析数千个细胞。 数千个细胞可以包含混合的细胞群体（例如，不同类型或亚型的细胞，不同大小的细胞）。

公开(公告)号：US11220685B2

公开(公告)日：2022-01-11

申请号：US16836750

申请日：2020-03-31

Applicant: Cellular Research, Inc.

Inventor： Glenn Fu ,  Eleen Shum

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6844

Abstract: The present disclosure relates to compositions, methods and kits for labeling an internal sequence of a target nucleic acid molecule with molecular barcodes. In some embodiments, the methods comprise intramolecular circulation of a labeled target nucleic acid molecule. Further provided methods for generating sequencing libraries comprising overlapping fragments covering the full length of a target nucleic acid molecule, sequencing the libraries using the methods disclosed herein, and methods of analyzing sequencing results therefrom.

公开(公告)号：US20180371536A1

公开(公告)日：2018-12-27

申请号：US15574122

申请日：2016-05-26

Applicant: Cellular Research, Inc.

Inventor： Glenn Fu ,  Julie Wilhelmy

IPC: C12Q1/6851 ,  C07H21/02

Abstract: The disclosure provides for methods, compositions, systems, devices, and kits for quantification of RNA, and determination of efficiency of re verse transcription of RNA to cDNA.

公开(公告)号：US10151003B2

公开(公告)日：2018-12-11

申请号：US16038979

申请日：2018-07-18

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P. A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68 ,  C12Q1/6888 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6874

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).