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公开(公告)号：US20190226000A1
公开(公告)日：2019-07-25
申请号：US16299960
申请日：2019-03-12
Applicant: DSM IP ASSETS B.V.
Inventor： Nicolette Jasmijn BROERS , Viktor Marius BOER , Adam G. LAWRENCE
IPC: C12P19/56 , C12P15/00 , C12N9/02 , C12N9/10 , C07H15/256 , C07C62/32 , A23L27/30 , A23L2/60 , C12N15/52 , C12N9/90 , C12N9/88
Abstract: The present invention relates to a method for the production of a diterpene or a glycosylated diterpene, which method comprises: a. fermenting a recombinant microorganism of the genus Yarrowia in a suitable fermentation medium at a temperature of about 29° C. or higher, wherein the microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity and whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol; and b. recovering the diterpene or glycosylated diterpene.
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公开(公告)号：US20220090032A1
公开(公告)日：2022-03-24
申请号：US17541565
申请日：2021-12-03
Applicant: DSM IP ASSETS B.V.
Abstract: The present invention relates a variant polypeptide having geranylgeranyl pyrophosphate synthase activity, which variant polypeptide comprises an amino acid sequence which, when aligned with a geranylgeranyl pyrophosphate synthase comprising the sequence set out in SEQ ID NO: 1, comprises at least one substitution of an amino acid residue corresponding to any of amino acids at positions 92, 100 or 235 said positions being defined with reference to SEQ ID NO: 1 and wherein the variant has one or more modified properties as compared with a reference polypeptide having geranylgeranyl pyrophosphate synthase activity. A variant polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.
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公开(公告)号：US20210348136A1
公开(公告)日：2021-11-11
申请号：US17381479
申请日：2021-07-21
Applicant: DSM IP ASSETS B.V.
Abstract: The present invention relates a polypeptide having kaurenoic acid 13-hydroxylase activity, which polypeptide comprises an amino acid sequence which, when aligned with a kaurenoic acid 13-hydroxylase comprising the sequence set out in SEQ ID NO: 1 or SEQ ID NO: 3, comprises at least one substitution of an amino acid corresponding to any of amino acids at positions 136, 248, 336 or 403, said positions being defined with reference to SEQ ID NO: 1 or SEQ ID NO: 3 and wherein the polypeptide has one or more modified properties as compared with a reference polypeptide having kaurenoic acid 13-hydroxylase activity. A polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.
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Patent Agency Ranking
公开(公告)号：US20200283816A1
公开(公告)日：2020-09-10
申请号：US16845736
申请日：2020-04-10
Applicant: DSM IP ASSETS B.V.
Inventor： Viktor Marius BOER , Priscilla Zwartjens , Eric Van Den Berg
Abstract: A recombinant host capable of producing a steviol glycoside which overexpresses a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 35 or SEQ ID NO: 38 or an amino acid sequence having at least about 50% sequence identity to either thereto. A recombinant host capable of producing a steviol glycoside which has been modified, preferably in its genome, to result in a deficiency in the production of a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 35 or SEQ ID NO: 38 or an amino acid sequence having at least about 50% sequence identity to either thereto.
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公开(公告)号：US20180057850A1
公开(公告)日：2018-03-01
申请号：US15560318
申请日：2016-03-23
Applicant: DSM IP ASSETS B.V.
Inventor： Hendrik Jan BOSCH , Martinus Julius BEEKWILDER , Viktor Marius BOER
CPC classification number: C12P19/56 , A23L2/60 , A23L27/36 , A23V2002/00 , C12N9/0042 , C12N9/1048 , C12N9/1051 , C12Y106/02004 , C12Y204/01017
Abstract: The present invention relates to polypeptides having UDP-Glycosyltransferase activity derived from Solanum lycopersicum and having the amino acid sequence set out in any of SEQ ID NO: 1 to 4 or an amino acid sequence having at least about 30% sequence identity thereto. The application also relates to recombinant hosts comprising a recombinant nucleic acid sequence encoding said polypeptides and uses thereof prepare glycosylated diterpenes, like steviol glycoside. The host cells might comprise further enzymes of the steviol glycoside biosynthesis pathway.
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公开(公告)号：US20160177360A1
公开(公告)日：2016-06-23
申请号：US14893216
申请日：2014-06-02
Applicant: DSM IP ASSETS B.V.
Inventor： Viktor Marius BOER , Erwin SUIR
IPC: C12P19/56 , C07H15/256
Abstract: The present invention relates to a recombinant microorganism comprising one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity, whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol. The recombinant microorganism may also be capable of expressing one or more UDP-glucosyltransferases such that the microorganism is capable of producing one or more steviol glycosides.
Abstract translation: 本发明涉及包含一个或多个核苷酸序列的重组微生物，所述核苷酸序列编码：具有入 - copalyl焦磷酸合成酶活性的多肽; 具有ent-Kaurene合酶活性的多肽; 具有ent-Kaurene氧化酶活性的多肽; 和具有海藻酸13-羟化酶活性的多肽，由此所述核苷酸序列的表达赋予所述微生物至少产生甜菊醇的能力。 重组微生物还可能能够表达一种或多种UDP-葡糖基转移酶，使得微生物能够产生一种或多种甜菊糖苷。
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公开(公告)号：US20160160257A1
公开(公告)日：2016-06-09
申请号：US14906497
申请日：2014-07-23
Applicant: DSM IP ASSETS B.V.
Inventor： Nicolette Jasmijn BROERS , Viktor Marius BOER , Adam G. LAWRENCE
CPC classification number: C12P19/56 , A23L2/60 , A23L27/36 , C07C62/32 , C07C2603/86 , C07H15/256 , C12N9/0042 , C12N9/0073 , C12N9/0083 , C12N9/1051 , C12N9/88 , C12N9/90 , C12N15/52 , C12P15/00 , C12Y114/13078 , C12Y114/13079 , C12Y402/03019 , C12Y505/01013
Abstract: The present invention relates to a method for the production of a diterpene or a glycosylated diterpene, which method comprises: a. fermenting a recombinant microorganism of the genus Yarrowia in a suitable fermentation medium at a temperature of about 29° C. or higher, wherein the microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity and whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol; and b. recovering the diterpene or glycosylated diterpene.
Abstract translation: 本发明涉及二萜或糖基二萜的制备方法，该方法包括：a。 在约29℃或更高的温度下在合适的发酵培养基中发酵耶氏酵母属的重组微生物，其中所述微生物包含一个或多个核苷酸序列，所述核苷酸序列编码：具有共 - 酰基焦磷酸合酶活性的多肽; 具有ent-Kaurene合酶活性的多肽; 具有ent-Kaurene氧化酶活性的多肽; 和具有山梨酸13-羟化酶活性的多肽，由此所述核苷酸序列的表达赋予所述微生物至少产生甜菊醇的能力; 和b。 回收二萜或糖基化二萜。
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公开(公告)号：US20160153017A1
公开(公告)日：2016-06-02
申请号：US14904030
申请日：2014-07-15
Applicant: DSM IP ASSETS B.V.
IPC: C12P19/56 , C12N15/81 , C07H15/256
CPC classification number: C12P19/56 , A23G3/36 , A23G4/06 , A23G9/32 , A23L9/10 , A23L21/10 , A23L23/00 , A23L27/36 , A23L27/60 , A23V2002/00 , C07H15/256 , C12N15/52 , C12N15/81 , C12N15/815 , C12P5/007
Abstract: The present invention relates to a recombinant microorganism whereby expression of nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least rebaudioside M. Such a recombinant microorganism may be used in a method of production of rebaudioside M.
Abstract translation: 本发明涉及一种重组微生物，其中核苷酸序列的表达赋予微生物至少产生莱鲍迪苷M的能力。这种重组微生物可用于生产新蛇菊苷M.
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