公开(公告)号：US20060013832A1

公开(公告)日：2006-01-19

申请号：US11029188

申请日：2005-01-04

申请人： Carl June ,  Craig Thompson ,  Gary Nabel ,  Gary Gray ,  Paul Rennert

发明人： Carl June ,  Craig Thompson ,  Gary Nabel ,  Gary Gray ,  Paul Rennert

IPC分类号： A61K39/21 ,  C12N15/86

CPC分类号： C12N5/0636 ,  A61K35/17 ,  A61K38/00 ,  A61K39/02 ,  A61K39/12 ,  A61K48/00 ,  A61K2039/505 ,  A61K2039/507 ,  A61K2039/5158 ,  C07K14/705 ,  C07K14/70521 ,  C07K14/70532 ,  C07K16/00 ,  C07K16/2806 ,  C07K16/2809 ,  C07K16/2812 ,  C07K16/2815 ,  C07K16/2818 ,  C07K16/2833 ,  C07K16/2866 ,  C07K16/289 ,  C07K16/2896 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/54 ,  C07K2317/55 ,  C07K2317/73 ,  C07K2317/74 ,  C07K2319/00 ,  C07K2319/30 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2501/599 ,  C12N2710/00088 ,  C12N2740/16011

摘要： Methods for inducing a population of T cells to proliferate by activating the population of T cells and stimulating an accessory molecule on the surface of the T cells with a ligand which binds the accessory molecule are described. T cell proliferation occurs in the absence of exogenous growth factors or accessory cells. T cell activation is accomplished by stimulating the T cell receptor (TCR)/CD3 complex or the CD2 surface protein. To induce proliferation of an activated population T cells, an accessory molecule on the surface of the T cells, such as CD28, is stimulated with a ligand which binds the accessory molecule. The T cell population expanded by the method of the invention can be genetically transduced and used for immunotherapy or can be used in methods of diagnosis.

摘要翻译： 描述了通过激活T细胞群并用结合附属分子的配体刺激T细胞表面上的辅助分子来诱导T细胞群体增殖的方法。 T细胞增殖发生在没有外源生长因子或附属细胞的情况下。 通过刺激T细胞受体（TCR）/ CD3复合物或CD2表面蛋白来完成T细胞活化。 为了诱导活化的群体T细胞的增殖，T细胞表面上的辅助分子如CD28被与配合分子结合的配体刺激。 通过本发明的方法扩增的T细胞群体可以被遗传转导并用于免疫治疗，或可用于诊断方法。

公开(公告)号：US20090191235A1

公开(公告)日：2009-07-30

申请号：US12065894

申请日：2006-09-06

申请人： Peter Kwong ,  John Mascola ,  Gary Nabel ,  Richard Wyatt ,  Barna Dey ,  Ling Xu ,  Tongqing Zhou ,  Chih-Chin Huang ,  Joseph Sodroski ,  Wen Yuan ,  Shi-Hua Xiang

发明人： Peter Kwong ,  John Mascola ,  Gary Nabel ,  Richard Wyatt ,  Barna Dey ,  Ling Xu ,  Tongqing Zhou ,  Chih-Chin Huang ,  Joseph Sodroski ,  Wen Yuan ,  Shi-Hua Xiang

IPC分类号： A61K39/21 ,  C07K14/16 ,  C07H21/04 ,  C12N15/63 ,  C12N5/00 ,  A61K31/7088

CPC分类号： C07K14/005 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/53 ,  A61K2039/54 ,  A61K2039/545 ,  C07K14/70514 ,  C12N2740/15022 ,  C12N2740/16122 ,  C12N2740/16134

摘要： Stabilized forms of gp120 polypeptide, nucleic acids encoding these stabilized forms, vectors comprising these nucleic acids, and methods of using these polypeptides, nucleic acids, vectors and host cells are disclosed. Crystal structures and computer systems including atomic coordinates for stabilized forms of gp120, and gp120 with an extended V3 loop, and methods of using these structures and computer systems are also disclosed.

摘要翻译： 公开了稳定形式的gp120多肽，编码这些稳定形式的核酸，包含这些核酸的载体，以及使用这些多肽，核酸，载体和宿主细胞的方法。 还公开了包括gp120的稳定形式的原子坐标和具有扩展的V3循环的gp120的晶体结构和计算机系统，以及使用这些结构和计算机系统的方法。

公开(公告)号：US20060286121A1

公开(公告)日：2006-12-21

申请号：US11337866

申请日：2006-01-23

申请人： Jason Gall ,  Thomas Wickham ,  William Enright ,  Douglas Brough ,  Mohammed Zuber ,  C. King ,  Gary Nabel ,  Cheng Cheng

发明人： Jason Gall ,  Thomas Wickham ,  William Enright ,  Douglas Brough ,  Mohammed Zuber ,  C. King ,  Gary Nabel ,  Cheng Cheng

IPC分类号： A61K39/12 ,  A61K39/395

CPC分类号： C12N15/86 ,  A61K48/00 ,  A61K2039/5256 ,  C07K2319/00 ,  C12N2710/10343 ,  C12N2710/10345

摘要： The invention provides a method of inducing an immune response in a mammal. The method comprises administering to the mammal a non-subgroup C adenoviral vector comprising an adenoviral fiber protein having an amino acid sequence comprising about 80% or more identity to an amino acid sequence encoding a subgroup C adenoviral fiber protein. The adenoviral vector further comprises a nucleic acid sequence encoding an antigen which is expressed in the mammal to induce an immune response. The invention further comprises a method of producing an adenoviral vector, and a composition comprising a serotype 41 or a serotype 35 adenoviral vector and a carrier. The invention also provides an adenoviral vector comprising a nucleic acid sequence encoding an adenoviral pIX protein operably linked to a heterologous expression control sequence, as well as a method of enhancing the stability and/or packaging capacity of an adenoviral vector.

摘要翻译： 本发明提供在哺乳动物中诱导免疫应答的方法。 该方法包括向哺乳动物施用非亚组C腺病毒载体，其包含具有与编码亚组C腺病毒纤维蛋白的氨基酸序列具有约80％或更高同一性的氨基酸序列的腺病毒纤维蛋白。 腺病毒载体还包含编码在哺乳动物中表达以诱导免疫应答的抗原的核酸序列。 本发明还包括产生腺病毒载体的方法和包含血清型41或血清型35腺病毒载体和载体的组合物。 本发明还提供了一种腺病毒载体，其包含编码与异源表达控制序列可操作地连接的腺病毒pIX蛋白的核酸序列，以及增强腺病毒载体的稳定性和/或包装能力的方法。

公开(公告)号：US20060269572A1

公开(公告)日：2006-11-30

申请号：US11332976

申请日：2006-01-17

申请人： Gary Nabel ,  Nancy Sullivan ,  Thomas Geisbert ,  Peter Jahrling

发明人： Gary Nabel ,  Nancy Sullivan ,  Thomas Geisbert ,  Peter Jahrling

IPC分类号： C12Q1/68 ,  A61K39/12

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/545 ,  C07K16/1081 ,  C12N7/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2760/14134 ,  C12N2760/14171 ,  Y02A50/464

摘要： The present invention relates to genetic vaccines for stimulating cellular and humoral immune responses in humans and other hosts, and, in particular, relates to recombinant viruses that express heterologous antigens of pathogenic viruses, in single dose form.

摘要翻译： 本发明涉及用于刺激人和其他宿主细胞和体液免疫应答的遗传疫苗，特别涉及以单剂量形式表达病原性病毒的异源抗原的重组病毒。

公开(公告)号：US20050281844A1

公开(公告)日：2005-12-22

申请号：US11038933

申请日：2005-01-19

申请人： Gary Nabel ,  Anthony Sanchez

发明人： Gary Nabel ,  Anthony Sanchez

IPC分类号： C07K14/08 ,  A61K48/00 ,  A61K39/12

CPC分类号： C07K14/005 ,  A61K2039/53 ,  C12N2760/14122

摘要： Ebola virus vaccines comprising nucleic acid molecules encoding Ebola viral proteins are provided. In one embodiment, the nucleic acid molecule encodes the transmembrane form of the viral glycoprotein (GP). In another embodiment, the nucleic acid molecule encodes the secreted form of the viral glycoprotein (sGP). In yet another embodiment, the nucleic acid molecule encodes the viral nucleoprotein (NP). Methods for immunizing a subject against disease caused by infection with Ebola virus are also provided.

摘要翻译： 提供包含编码埃博拉病毒蛋白的核酸分子的埃博拉病毒疫苗。 在一个实施方案中，核酸分子编码病毒糖蛋白（GP）的跨膜形式。 在另一个实施方案中，核酸分子编码病毒糖蛋白（sGP）的分泌形式。 在另一个实施方案中，核酸分子编码病毒核蛋白（NP）。 还提供了用于免疫针对由埃博拉病毒感染引起的疾病的方法。

公开(公告)号：US4720482A

公开(公告)日：1988-01-19

申请号：US870835

申请日：1986-06-05

申请人： Harvey I. Cantor ,  Gary Nabel

发明人： Harvey I. Cantor ,  Gary Nabel

IPC分类号： A61K38/00 ,  A61K47/48 ,  C07K14/52 ,  A61K37/02

CPC分类号： A61K47/48423 ,  C07K14/52 ,  A61K38/00

摘要： The present invention is directed to a pharmaceutical composition, comprising a mammalian cell growth factor and a pharmaceutically acceptable carrier. The growth factor of the present invention is selected from the group comprising;(a) a protein having a molecular weight of about 14 kilodaltons and being functional to stimulate growth of both B and T lymphocytes;(b) a protein having an apparent molecular weight of about 50 kilodaltons and being functional to stimulate growth of B lymphocytes; and(c) a growth factor having an apparent mean molecular weight of about 45 kilodaltons and being funtional to stimulate growth of mast cells.

摘要翻译： 本发明涉及包含哺乳动物细胞生长因子和药学上可接受的载体的药物组合物。 本发明的生长因子选自： （a）具有约14千道尔顿分子量的蛋白质，其功能是刺激B淋巴细胞和T淋巴细胞的生长; （b）表观分子量约为50千道尔顿并具有刺激B淋巴细胞生长功能的蛋白质; 和（c）具有约45千道尔顿的表观平均分子量并有助于刺激肥大细胞生长的生长因子。

公开(公告)号：US4613459A

公开(公告)日：1986-09-23

申请号：US435510

申请日：1982-10-20

申请人： Harvey I. Cantor ,  Gary Nabel

发明人： Harvey I. Cantor ,  Gary Nabel

IPC分类号： A61K38/00 ,  A61K47/48 ,  C07K14/52 ,  C07K3/28 ,  C12N5/00 ,  C12N5/02 ,  C12N1/38

CPC分类号： A61K47/48423 ,  C07K14/52 ,  A61K38/00

摘要： A family of growth factors, methods of making and using such growth factors in in vivo treatment of living mammalian cells, and products incorporating such growth factors, are disclosed.The growth factors were isolated from the supernatant fluid obtained by incubation of inducer T-cells in the presence of a suitable antigen or mitogen. The growth factors disclosed include a 14 Kd polypeptide which stimulates growth of both T-cells and B-cells, a 50 Kd polypeptide which stimulates B-cells to multiply and secrete immunoglobulins, and a 45 Kd growth factor which stimulates secretion of immunoglobulins by B-cells and stimulates rapid growth of certain mast cells, stem cells and certain other types of cells.

摘要翻译： 公开了一系列生长因子，制造和使用这些生长因子的活体哺乳动物细胞的体内治疗方法，以及结合这种生长因子的产品。 通过在合适的抗原或有丝分裂原存在下温育诱导物T细胞而获得的上清液中分离生长因子。 公开的生长因子包括刺激T细胞和B细胞生长的14Kd多肽，刺激B细胞增殖和分泌免疫球蛋白的50Kd多肽，以及刺激B免疫球蛋白分泌的45Kd生长因子 细胞并刺激某些肥大细胞，干细胞和某些其他类型细胞的快速生长。

公开(公告)号：US10273291B2

公开(公告)日：2019-04-30

申请号：US14116710

申请日：2012-03-23

申请人： John R. Mascola ,  Gary Nabel ,  Barton F. Haynes ,  Xueling Wu ,  Thomas B. Kepler ,  Peter Kwong

发明人： John R. Mascola ,  Gary Nabel ,  Barton F. Haynes ,  Xueling Wu ,  Thomas B. Kepler ,  Peter Kwong

IPC分类号： C07K16/10 ,  A61K39/42 ,  G01N33/569

摘要： Antibody VRC01 represents a human immunoglobulin that neutralizes—˜90% of diverse HIV-1 isolates. To understand how such broadly neutralizing HIV-1 antibodies develop and recognize the viral envelope, we used X-ray crystallography and 454 pyrosequencing to characterize additional antibodies from HIV-1-infected individuals. Crystal structures revealed a convergent mode of binding of different antibodies to the same CD4-binding-site epitope. Antibody recognition was achieved through the evolution of complementary contact domains that were generated in diverse ways. Phylogenetic analysis of expressed heavy and light chains determined by deep sequencing revealed a common pathway of antibody heavy chain maturation confined to IGHV1-2*02 lineage that could pair with different light chains. The maturation pathway inferred by antibodyomics reveals that diverse antibodies evolve to a highly affinity-matured state to recognize an invariant viral structure, providing insight into the development and evolution of broadly neutralizing HIV-1 immunity.

公开(公告)号：US20120034255A1

公开(公告)日：2012-02-09

申请号：US13232775

申请日：2011-09-14

申请人： Peter Kwong ,  John Mascola ,  Gary Nabel ,  Richard Wyatt ,  Barna Dey ,  Ling Xu ,  Tongqing Zhou ,  Joseph Sodroski ,  Wen Yuan ,  Shi-Hua Xiang

发明人： Peter Kwong ,  John Mascola ,  Gary Nabel ,  Richard Wyatt ,  Barna Dey ,  Ling Xu ,  Tongqing Zhou ,  Joseph Sodroski ,  Wen Yuan ,  Shi-Hua Xiang

IPC分类号： A61K39/21 ,  A61P31/18 ,  C07K14/16

CPC分类号： C07K14/005 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/53 ,  A61K2039/54 ,  A61K2039/545 ,  C07K14/70514 ,  C12N2740/15022 ,  C12N2740/16122 ,  C12N2740/16134

摘要： Stabilized forms of gp120 polypeptide, nucleic acids encoding these stabilized forms, vectors comprising these nucleic acids, and methods of using these polypeptides, nucleic acids, vectors and host cells are disclosed. Crystal structures and computer systems including atomic coordinates for stabilized forms of gp120, and gp120 with an extended V3 loop, and methods of using these structures and computer systems are also disclosed.

公开(公告)号：US07449190B2

公开(公告)日：2008-11-11

申请号：US11031921

申请日：2005-01-07

申请人： Gary Nabel ,  Yue Huang

发明人： Gary Nabel ,  Yue Huang

IPC分类号： A61K39/00

CPC分类号： G01N33/56983 ,  C12Q1/18

摘要： The present invention relates to assays for the identification of compounds that inhibit assembly of NP, VP35, and VP24, or inhibit the glycosylation of NP, required for nucleocapsid formation, for use as anti-viral agents. The invention also relates to assays for the identification of compounds that block glycosylation of proteins having a glycosylation domain that is substantially homologous to a glycosylation domain of NP required for polymerization. The invention further relates to pseudoparticles for presentation of antigens or antigenic epitopes for immunogenic or vaccination purposes.

摘要翻译： 本发明涉及用于鉴定抑制NP，VP35和VP24的组装或抑制核衣壳形成所需的NP的糖基化作为抗病毒剂的化合物的测定。 本发明还涉及用于鉴定封闭糖基化的化合物的测定法，其具有与聚合所需的NP的糖基化结构域基本上同源的糖基化结构域。 本发明还涉及用于呈递用于免疫原性或疫苗接种目的的抗原或抗原表位的伪颗粒。