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28 条记录 (耗时 0.036 秒)

    Method for isolating a target biological material, capture phase, detecting phase and reagent containing them
    24.
    发明授权
    Method for isolating a target biological material, capture phase, detecting phase and reagent containing them 有权
    用于分离靶生物材料，捕获相，检测相和含有它们的试剂的方法

    公开(公告)号：US06632615B2

    公开(公告)日：2003-10-14

    申请号：US09230846

    申请日：1999-04-21

    申请人： Francois Mallet ,  Thierry DeLair ,  Catherine Ladaviere ,  Armelle Novelli-Rousseau ,  Marie-Helene Charles

    发明人： Francois Mallet ,  Thierry DeLair ,  Catherine Ladaviere ,  Armelle Novelli-Rousseau ,  Marie-Helene Charles

    IPC分类号： G01N3353

    CPC分类号： G01N33/54393 ,  C12N15/62 ,  C12N15/87

    摘要： The invention concerns a method for isolating a target biological material contained in a sample, which consists in providing a capture phase comprising an organic molecule having at least a reactive function and at least a protein material capable of recognizing or binding, specifically and directly or indirectly, with the target biological material, said protein material having a specific covalent binding site with the organic molecule reactive function, consisting of at least a tag comprising at least six contiguous lysine, or lysine derivative residues, the method consists in contacting said target biological material with at least the capture phase; and detecting the target biological material fixed on the capture phase: The invention also concerns the capture and detection phases, and a reagent containing them.

    摘要翻译： 本发明涉及一种用于分离样品中包含的靶生物材料的方法，其包括提供包含至少具有反应性功能的有机分子和至少能够识别或结合，特异性和直接或间接的蛋白质材料的捕获相 与目标生物材料一起，所述蛋白质材料具有与有机分子反应性功能的特定共价结合位点，由至少包含至少六个相邻赖氨酸或赖氨酸衍生物残基的标签组成，该方法包括使所述靶生物材料 至少有捕获阶段; 并检测固定在捕获相上的靶生物材料：本发明还涉及捕获和检测阶段以及含有它们的试剂。

    Cell lines and viral isolates associated with multiple sclerosis
    25.
    发明授权
    Cell lines and viral isolates associated with multiple sclerosis 有权
    与多发性硬化相关的细胞系和病毒分离物

    公开(公告)号：US06342383B1

    公开(公告)日：2002-01-29

    申请号：US09133411

    申请日：1998-08-13

    申请人： Herve Perron ,  Francois Mallet ,  Bernard Mandrand ,  Frederic Bedin ,  Frederic Beseme

    发明人： Herve Perron ,  Francois Mallet ,  Bernard Mandrand ,  Frederic Bedin ,  Frederic Beseme

    IPC分类号： C12N112

    CPC分类号： C07K14/005 ,  A61K38/00 ,  C12N7/00 ,  C12N2740/10021 ,  C12N2740/10022 ,  Y10S435/912

    摘要： Composition comprising two pathogenic and/or infective agents associated with multiple sclerosis, namely a first agent which consists of a human virus possessing reverse transcriptase activity and related to a family of endogenous retroviral elements, or a variant of said virus, and a second agent, or a variant of said second agent, these two pathogenic and/or infective agents originating from the same viral strain chosen from the strains designated, respectively, POL-2 deposited with the ECACC on Jul. 22 1992 under Accession Number V92072202 and MS7PG deposited with the ECACC on Jan. 8 1993 under Accession Number V93010816, and from their variant strains.

    摘要翻译： 包含与多发性硬化相关的两种致病和/或感染因子的组合物，即由具有逆转录酶活性并与内源性逆转录病毒元件家族相关的人病毒或所述病毒的变体组成的第一药剂和第二药剂， 或所述第二药剂的变体，这两种源自相同病毒株的两种致病和/或感染剂分别选自1992年7月22日以保藏号V92072202和EC7PG保藏的ECACC分别保藏的POL-2的菌株 1993年1月8日的ECACC，保藏号V93010816，以及他们的变异株。

    Modified promoter for RNA polymerase, its preparation and its applications
    26.
    发明授权
    Modified promoter for RNA polymerase, its preparation and its applications 失效
    RNA聚合酶的修饰启动子，其制备及其应用

    公开(公告)号：US5891681A

    公开(公告)日：1999-04-06

    申请号：US360051

    申请日：1994-12-20

    申请人： Francois Mallet ,  Francoise Guillou-Bonnici ,  Philippe Cleuziat ,  Pierre Levasseur

    发明人： Francois Mallet ,  Francoise Guillou-Bonnici ,  Philippe Cleuziat ,  Pierre Levasseur

    IPC分类号： C12N15/11 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/70 ,  C07H21/04

    CPC分类号： C12P19/34 ,  C12N15/11 ,  C12Q1/6865 ,  C12Q1/703

    摘要： An oligonucleotide is intended to be used as a promoter non-template strand in the transcription of a sequence of a nucleotide target in the presence of a phage RNA polymerase. The phage RNA polymerase has specific natural promoters containing a consensus sequence from at least position -17 to position -1. The oligonucleotide contains a core sequence flanked at at least one of its ends by a nucleotide sequence capable of hybridization with a sequence of the target. The core sequence contains a sequence of 6 to 9 consecutive nucleotides from the region -12 to -4 of the non-template strand of the specific promoter, or a sufficiently homologous sequence to enable the functionality of the RNA polymerase to be retained. One flanking sequence is complementary to a first region of the target, and a second flanking sequence, when present, is complementary to a second region of the target, the first and second regions being separated on the target by a sequence having a number of nucleotides equal to the number of nucleotides in the core sequence. The number of nucleotides in the flanking region, or the sum of the number of nucleotides in the flanking regions, is at least sufficiently high for the nucleotide to be able to hybridize with the target at the temperature of use of the RNA polymerase. Such an oligonucleotide enables transcription to be initiated at a site of the target which is not normally a transcription start site for the RNA polymerase.

    摘要翻译： 在噬菌体RNA聚合酶存在下，将寡核苷酸用作核苷酸靶序列转录中的启动子非模板链。 噬菌体RNA聚合酶具有特异性天然启动子，其含有至少从-17位置到-1位的共有序列。 寡核苷酸包含核苷酸序列，其核苷酸序列在其至少一个末端具有能够与靶序列杂交的核苷酸序列。 核心序列包含从特异性启动子的非模板链的-12至-4区的6至9个连续核苷酸的序列，或充分同源的序列，以使RNA聚合酶的功能得以保留。 一个侧翼序列与靶的第一区互补，当存在时，第二侧翼序列与靶的第二区互补，第一和第二区通过具有多个核苷酸的序列在靶上分离 等于核心序列中核苷酸的数目。 侧翼区域中的核苷酸数目或侧翼区域中的核苷酸数量的总和至少足以使核苷酸能够在RNA聚合酶的使用温度下与靶标杂交。 这样的寡核苷酸使得能够在目标的位点起始转录，该位点通常不是RNA聚合酶的转录起始位点。

    Method for nucleic acid amplification by transcription using displacement, and reagents and kit therefor
    27.
    发明授权
    Method for nucleic acid amplification by transcription using displacement, and reagents and kit therefor 失效
    通过使用位移转录进行核酸扩增的方法及其试剂及试剂盒

    公开(公告)号：US5849547A

    公开(公告)日：1998-12-15

    申请号：US406849

    申请日：1995-04-24

    申请人： Philippe Cleuziat ,  Francoise Guillou-Bonnici ,  Francois Mallet ,  Pierre Levasseur

    发明人： Philippe Cleuziat ,  Francoise Guillou-Bonnici ,  Francois Mallet ,  Pierre Levasseur

    IPC分类号： C12N15/09 ,  C12Q1/68 ,  C12P19/34 ,  C07H21/04

    CPC分类号： C12Q1/6853 ,  C12Q1/6844 ,  C12Q1/6846

    摘要： A target nucleic acid sequence is amplified by providing a polynucleotide such as VII including the sequence to be amplified as well as an RNA polymerase promoter sequence, and contacting said polynucleotide, in the presence of a system having the RNA polymerase activity, a RNA-dependent DNA polymerase activity, and a DNA-dependent DNA polymerase activity and capable of strand displacement, with a set of primers. The primers include a primer such as A capable of hybridizing with a segment complementary to a portion of the sequence to be amplified, wherein A includes an upstream RNA polymerase promoter sequence followed by an arbitrary sequence, a primer such as D containing said RNA polymerase promoter sequence, and primer such as C containing said arbitrary sequence. The elongation product of D is displaced by the elongation product of C, and the elongation product of D is thus obtained in the form of single-stranded VIII. Hybridization of A on VIII followed by elongation forms double stranded product X whiuch can undergo transcription, with amplification, to give single-stranded RNA VII bis. An analogous system of 3 primers B, E and F similarly produces single-stranded DNA VII from VII bis. Hence, the amplification method, which can operate isothermally, is cyclic.

    摘要翻译： PCT No.PCT / FR94 / 00935 Sec。 371日期1995年04月24日 102（e）1995年4月24日PCT PCT 1994年7月26日PCT公布。 出版物WO95 / 03426 日期1995年2月2日通过提供包括待扩增的序列的多核苷酸，以及RNA聚合酶启动子序列扩增靶核酸序列，并在具有RNA聚合酶活性的系统的存在下使所述多核苷酸接触 ，RNA依赖性DNA聚合酶活性和DNA依赖性DNA聚合酶活性，并能够用一组引物进行链置换。 引物包括能够与与待扩增的序列部分互补的区段杂交的引物，其中A包括上游RNA聚合酶启动子序列，随后任意序列，引物如含有所述RNA聚合酶启动子的D 序列和引物，如含有所述任意序列的C。 D的伸长率由C的伸长率移位，因此以单链VIII的形式得到D的伸长率。 A对VIII的杂交随后延伸形成双链产物X，其可以进行转录，扩增，得到单链RNA VII bis。 3个引物B，E和F的类似系统类似地从VII bis产生单链DNA VII。 因此，可以等温操作的扩增方法是循环的。