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公开(公告)号:US20090098610A1
公开(公告)日:2009-04-16
申请号:US11628268
申请日:2005-05-12
申请人: Yoshimi Sato , Kazue Nishiwaki , Nana Shimada , Shigekazu Hokazono , Takashi Uemori , Hiroyuki Mukai , Ikunoshin Kato
发明人: Yoshimi Sato , Kazue Nishiwaki , Nana Shimada , Shigekazu Hokazono , Takashi Uemori , Hiroyuki Mukai , Ikunoshin Kato
CPC分类号: C12Q1/686 , C12N9/1252 , C12Q2521/101
摘要: A polypeptide having a high fidelity DNA polymerase activity and thus being useful as a genetic engineering reagent; a gene encoding this polypeptide; a method of producing the polypeptide; and a method of amplifying a nucleic acid by using the polypeptide.
摘要翻译: 具有高保真DNA聚合酶活性的多肽,因此可用作遗传工程试剂; 编码该多肽的基因; 一种产生该多肽的方法; 以及通过使用多肽扩增核酸的方法。
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公开(公告)号:US07422888B2
公开(公告)日:2008-09-09
申请号:US10380430
申请日:2001-09-13
申请人: Takashi Uemori , Yoshimi Sato , Nobuto Koyama , Ryo Hirano , Hikaru Takakura , Hiroshi Kobori , Yuji Hashimoto , Kiyozo Asada , Ikunoshin Kato
发明人: Takashi Uemori , Yoshimi Sato , Nobuto Koyama , Ryo Hirano , Hikaru Takakura , Hiroshi Kobori , Yuji Hashimoto , Kiyozo Asada , Ikunoshin Kato
CPC分类号: C12N9/22
摘要: Polypeptides having an RNase H activity highly useful in genetic engineering; genes encoding these polypeptides; and a process for genetic engineeringly producing these polypeptides.
摘要翻译: 具有在遗传工程中非常有用的RNase H活性的多肽; 编码这些多肽的基因; 以及用于遗传工程化生产这些多肽的方法。
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公开(公告)号:US20050239100A1
公开(公告)日:2005-10-27
申请号:US10973919
申请日:2004-10-27
申请人: Hiroyuki Mukai , Hiroaki Sagawa , Takashi Uemori , Junko Yamamoto , Jun Tomono , Eiji Kobayashi , Tatsuji Enoki , Osamu Takeda , Kazue Miyake , Yoshimi Sato , Mariko Moriyama , Haruhisa Sawaragi , Michio Hagiya , Kiyozo Asada , Ikunoshin Kato
发明人: Hiroyuki Mukai , Hiroaki Sagawa , Takashi Uemori , Junko Yamamoto , Jun Tomono , Eiji Kobayashi , Tatsuji Enoki , Osamu Takeda , Kazue Miyake , Yoshimi Sato , Mariko Moriyama , Haruhisa Sawaragi , Michio Hagiya , Kiyozo Asada , Ikunoshin Kato
CPC分类号: C12N9/1252 , C12P19/34 , C12Q1/6853 , C12Q2531/119 , C12Q2525/121 , C12Q2521/301
摘要: A convenient and effective method for amplifying a nucleic acid sequence characterized by effecting a DNA synthesis reaction in the presence of chimeric oligonucleotide primers; a method for supplying a large amount of DNA amplification fragments; an effective method for amplifying a nucleic acid sequence by combining the above method with another nucleic acid sequence amplification method; a method for detecting a nucleic acid sequence for detecting or quantitating a microorganism such as a virus, a bacterium, a fungus or a yeast; and a method for detecting a DNA amplification fragment obtained by the above method in situ.
摘要翻译: 一种用于扩增核酸序列的方法和方法,其特征在于在嵌合寡核苷酸引物存在下进行DNA合成反应; 提供大量DNA扩增片段的方法; 通过将上述方法与另一种核酸序列扩增方法结合来扩增核酸序列的有效方法; 用于检测用于检测或定量微生物如病毒,细菌,真菌或酵母的核酸序列的方法; 以及通过上述方法原位检测DNA扩增片段的方法。
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公开(公告)号:US20050123950A1
公开(公告)日:2005-06-09
申请号:US10929759
申请日:2004-08-31
申请人: Hiroyuki Mukai , Hiroaki Sagawa , Takashi Uemori , Junko Yamamoto , Jun Tomono , Eiji Kobayashi , Tatsuji Enoki , Osamu Takeda , Kazue Miyake , Yoshimi Sato , Mariko Moriyama , Haruhisa Sawaragi , Michio Hagiya , Kiyozo Asada , Ikunoshin Kato
发明人: Hiroyuki Mukai , Hiroaki Sagawa , Takashi Uemori , Junko Yamamoto , Jun Tomono , Eiji Kobayashi , Tatsuji Enoki , Osamu Takeda , Kazue Miyake , Yoshimi Sato , Mariko Moriyama , Haruhisa Sawaragi , Michio Hagiya , Kiyozo Asada , Ikunoshin Kato
CPC分类号: C12N9/1252 , C12P19/34 , C12Q1/6853 , C12Q2531/119 , C12Q2525/121 , C12Q2521/301
摘要: A convenient and effective method for amplifying a nucleic acid sequence characterized by effecting a DNA synthesis reaction in the presence of chimeric oligonucleotide primers; a method for supplying a large amount of DNA amplification fragments; an effective method for amplifying a nucleic acid sequence by combining the above method with another nucleic acid sequence amplification method; a method for detecting a nucleic acid sequence for detecting or quantitating a microorganism such as a virus, a bacterium, a fungus or a yeast; and a method for detecting a DNA amplification fragment obtained by the above method in situ.
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公开(公告)号:US06333158B1
公开(公告)日:2001-12-25
申请号:US09712266
申请日:2000-11-15
申请人: Takashi Uemori , Yoshimi Sato , Tomoko Fujita , Kazue Miyake , Hiroyuki Mukai , Kiyozo Asada , Ikunoshin Kato
发明人: Takashi Uemori , Yoshimi Sato , Tomoko Fujita , Kazue Miyake , Hiroyuki Mukai , Kiyozo Asada , Ikunoshin Kato
IPC分类号: C12Q168
CPC分类号: C12N9/1252
摘要: The present invention relates to a thermostable DNA polymerase-associated factor capable of enhancing DNA synthesizing-activity of a DNA polymerase; a thermostable DNA polymerase-associated factor possessing an activity of binding to a DNA polymerase and a method for producing the same; a gene encoding the DNA polymerase-associated factor; a method of DNA synthesis by using a DNA polymerase in the presence of the DNA polymerase-associated factor; and a kit comprising the DNA polymerase-associated factor. According to the present invention, there can be provided in vitro DNA synthesis and a DNA amplification system which are more excellent than conventional techniques by utilizing the DNA polymerase-associated factor of the present invention.
摘要翻译: 本发明涉及能够增强DNA聚合酶的DNA合成活性的热稳定的DNA聚合酶相关因子; 具有与DNA聚合酶结合的活性的热稳定DNA聚合酶相关因子及其制备方法; 编码DNA聚合酶相关因子的基因; 在DNA聚合酶相关因子存在下使用DNA聚合酶进行DNA合成的方法; 以及包含DNA聚合酶相关因子的试剂盒。 根据本发明,通过利用本发明的DNA聚合酶相关因子,可以提供比常规技术更优异的体外DNA合成和DNA扩增系统。
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公开(公告)号:US4434999A
公开(公告)日:1984-03-06
申请号:US301121
申请日:1981-09-11
申请人: Yoshimi Sato
发明人: Yoshimi Sato
IPC分类号: B60R21/045 , B60R21/02
CPC分类号: B60R21/045
摘要: A leg protector of an automotive vehicle, comprises first and second side plate members securely connected to a vehicle body and located generally between a steering column and legs of a driver, the first and second side plate members being laterally aligned with and spaced apart from each other, and a central plate member located facing the steering column and fastened onto the first and second side plate members, forming connecting sections. The mechanical strength of at least one of the central plate member and each connecting section is set less than that of first and second side plate members.
摘要翻译: 一种机动车辆的腿部保护器,包括牢固地连接到车身并且大致位于驾驶员的转向柱和腿之间的第一和第二侧板构件,第一和第二侧板构件与每个侧向侧向对准并间隔开 以及中心板构件,其定位成面向转向柱并且紧固在第一和第二侧板构件上,形成连接部分。 中心板构件和每个连接部分中的至少一个的机械强度被设定为小于第一和第二侧板构件的机械强度。
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公开(公告)号:US4381799A
公开(公告)日:1983-05-03
申请号:US224015
申请日:1981-01-12
申请人: Yoshimi Sato , Nobuo Hiraiwa , Akira Hasegawa
发明人: Yoshimi Sato , Nobuo Hiraiwa , Akira Hasegawa
CPC分类号: B62D5/087 , Y10T137/86702 , Y10T137/8671
摘要: A changeover valve unit for a power-assisted steering system includes input and output shafts arranged for relative rotation on aligned axes, a torsion bar interconnecting the shafts, a spool valve element surrounding the input shaft and arranged to be axially displaced in response to relative rotation of the shafts for controlling the supply of pressurized fluid to a hydraulic cylinder associated with the steering system, and a displacement mechanism for the spool valve element. The valve unit further includes an annular retainer member engaged with one end of the spool valve element to form an oil chamber, a compression spring assembled within the oil chamber to bias the spool valve element toward the displacement mechanism, and an annular orifice formed around the input shaft by means of the retainer member to permit the flow of fluid between the oil chamber and the interior of the spool valve element.
摘要翻译: 用于动力辅助转向系统的转换阀单元包括布置成在对准轴线上相对旋转的输入和输出轴,连接轴的扭力杆,围绕输入轴的滑阀元件,并被布置为响应于相对旋转而轴向移位 用于控制向与转向系统相关联的液压缸供应加压流体的轴以及用于滑阀元件的位移机构。 阀单元还包括与滑阀元件的一端接合以形成油室的环形保持器构件,组装在油室内的压缩弹簧以将滑阀元件朝向位移机构偏置,以及环形孔 通过保持器构件输入轴,以允许油室和滑阀元件内部之间的流体流动。
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公开(公告)号:US4355442A
公开(公告)日:1982-10-26
申请号:US199849
申请日:1980-10-23
申请人: Yoshimi Sato
发明人: Yoshimi Sato
CPC分类号: A44B11/2534 , Y10T24/4567
摘要: A connecting device for an automobile seat belt including a tag, and a buckle comprising a base and a slider block slidably fitted with each other. The base comprises at least two latches slidable in directions substantially at right angles to the direction of insertion of the tang and urged away from each other by means of a spring fixed at the center of the base and integrally constructed with a spring forcing the tang out of the buckle when the former is released from the latter. The slide block is provided with a guide hole engaging the latches of the base and having guides for moving the latches toward each other in response to a movement of the slider block. The tang has a pair of guides for moving the latches toward each other in response to insertion of the tang into the buckle and a pair of anchoring portions adjoining the pair of guides for the latches. The device including the buckle and tang is lengthwise shorten and compactly constructed, which is particularly advantageous for a passive seat belt.
摘要翻译: 一种用于包括标签的汽车座椅安全带的连接装置,以及包括可滑动地彼此嵌合的基部和滑动块的带扣。 底座包括至少两个闩锁,其可以在大致与柄脚插入方向成直角的方向上滑动,并通过固定在基座中心处的弹簧相互远离,并且一体地构造有弹簧,迫使脚尖 当前者从后者释放时,扣环。 滑动块设置有与基座的闩锁接合的引导孔,并且具有用于响应于滑块的移动而使闩锁朝向彼此移动的引导件。 脚具有一对引导件,用于响应于将柄插入到带扣中而使闩锁朝向彼此移动,以及一对与闩锁的引导件相邻的锚定部分。 包括带扣和脚的装置纵向缩短并紧凑地构造,这对于被动安全带是特别有利的。
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29.
公开(公告)号:US20120164654A1
公开(公告)日:2012-06-28
申请号:US13393302
申请日:2010-08-27
申请人: Yuko Nakabayashi , Yoshimi Sato , Takashi Uemori , Hiroyuki Mukai
发明人: Yuko Nakabayashi , Yoshimi Sato , Takashi Uemori , Hiroyuki Mukai
CPC分类号: C12Q1/686 , C12N15/1096 , C12Q2527/125 , C12Q2521/107
摘要: A composition for a reverse transcription polymerase chain reaction, which comprises a thermostable DNA polymerase, a reverse transcriptase, a dye marker and a specific gravity-increasing agent; and a premix reagent for a one-step RT-PCR, which comprises the composition, is not frozen under usual storage conditions at −20 to −30° C. and has excellent handleability.
摘要翻译: 用于逆转录聚合酶链反应的组合物,其包含热稳定性DNA聚合酶,逆转录酶,染料标记物和比重增加剂; 并且包含该组合物的用于一步RT-PCR的预混合试剂在-20至-30℃的通常储存条件下不冷冻,并且具有优异的可操作性。
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公开(公告)号:US08048987B2
公开(公告)日:2011-11-01
申请号:US12708111
申请日:2010-02-18
申请人: Yoshimi Sato , Kazue Nishiwaki , Nana Shimada , Shigekazu Hokazono , Takashi Uemori , Hiroyuki Mukai , Ikunoshin Kato
发明人: Yoshimi Sato , Kazue Nishiwaki , Nana Shimada , Shigekazu Hokazono , Takashi Uemori , Hiroyuki Mukai , Ikunoshin Kato
CPC分类号: C12Q1/686 , C12N9/1252 , C12Q2521/101
摘要: A polypeptide having a high fidelity DNA polymerase activity and thus being useful as a genetic engineering reagent; a gene encoding this polypeptide; a method of producing the polypeptide; and a method of amplifying a nucleic acid by using the polypeptide.
摘要翻译: 具有高保真DNA聚合酶活性的多肽,因此可用作遗传工程试剂; 编码该多肽的基因; 一种产生该多肽的方法; 以及通过使用多肽扩增核酸的方法。
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