公开(公告)号：US20230416803A1

公开(公告)日：2023-12-28

申请号：US18025794

申请日：2022-09-10

申请人： Illumina Cambridge Limited

发明人： Andrew Slatter

IPC分类号： C12Q1/6806 ,  C12N15/10

CPC分类号： C12Q1/6806 ,  C12N15/1068

摘要： Disclosed herein is a method for enriching a sequencing library comprising double-stranded nucleic acid fragments comprising preparing a library of double-stranded fragments having one or more adaptors at ends of the double-stranded fragment; denaturing the double-stranded fragments to form single-stranded fragments; and hybridizing an extension primer that binds to a target sequence of at least one insert in the library of double-stranded fragments and that does not bind to non-target sequences. In an embodiment, the adaptor is a hairpin adaptor, and extension from the extension primer using a polymerase with 5′ to 3′ exonuclease activity removes all or part of a sequence of the hairpin adaptor that is at least partially complementary to the amplification primer sequence. Each fragment may comprise an insert comprising double-stranded nucleic acid and a hairpin adaptor at the 5′ end of one or both strands of the double-stranded fragments. Hairpin adaptors may comprise an amplification primer sequence and a sequence at least partially complementary to the amplification primer sequence.

公开(公告)号：US20190078139A1

公开(公告)日：2019-03-14

申请号：US16163005

申请日：2018-10-17

申请人： Illumina Cambridge Limited

发明人： Louise Fraser ,  Paula Kokko-Gonzales ,  Andrew Slatter

IPC分类号： C12Q1/6806 ,  C12P19/34 ,  C12N15/10 ,  C12Q1/6844

摘要： Presented are methods and compositions for preparing samples for amplification and sequencing. Particular embodiments relate to methods of obtaining nucleic acids material directly from tissues such as whole blood or FFPE samples.

公开(公告)号：US20150353989A1

公开(公告)日：2015-12-10

申请号：US14733452

申请日：2015-06-08

申请人： Illumina Cambridge Limited

发明人： Louise Fraser ,  Paula Kokko-Gonzales ,  Andrew Slatter

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6806 ,  C12N15/1003 ,  C12P19/34 ,  C12Q1/6846 ,  C12Q2521/537 ,  C12Q2527/125 ,  C12Q2527/137 ,  C12Q2535/122 ,  C12Q2565/518

摘要： Presented are methods and compositions for preparing samples for amplification and sequencing. Particular embodiments relate to methods of obtaining nucleic acids material directly from tissues such as whole blood or FFPE samples.

摘要翻译： 提出了用于制备用于扩增和测序的样品的方法和组合物。 具体实施方案涉及直接从诸如全血或FFPE样品的组织获得核酸材料的方法。

公开(公告)号：US20240287583A1

公开(公告)日：2024-08-29

申请号：US18571069

申请日：2023-04-06

申请人： ILLUMINA, INC.

发明人： Andrew Slatter ,  Carlo Randise-Hinchliff ,  Andrew Price ,  Niall Anthony Gormley ,  Andrea Manzo ,  Nithya Subramanian ,  Fiona Kaper ,  David Jones ,  Steven Norberg

IPC分类号： C12Q1/682 ,  C12Q1/6839

CPC分类号： C12Q1/682 ,  C12Q1/6839

摘要： Aptamer detection techniques with dynamic range compression are described that permit removal of a portion of more abundant aptamers in an aptamer-based assay. In an embodiment, a mixture of tagged probes and dummy probes can be used such that the dummy probes bind abundant aptamers and in turn are not captured or amplified for detection in downstream steps. Other techniques are also contemplated, including targeted removal of or cleavage of probes that bind to excess aptamers.

公开(公告)号：US20240271126A1

公开(公告)日：2024-08-15

申请号：US18562095

申请日：2022-05-26

申请人： ILLUMINA, INC.

发明人： Niall Anthony Gormley ,  Carlo Randise-Hinchliff ,  Jeffrey Brodin ,  Esther Musgrave-Brown ,  Sarah E. Shultzaberger ,  Andrew Slatter ,  Jeffrey S. Fisher

IPC分类号： C12N15/10 ,  C12N15/11 ,  C12Q1/48

CPC分类号： C12N15/1096 ,  C12N15/11 ,  C12Q1/485

摘要： Nucleic acid techniques are disclosed. Embodiments include modified nucleotides with oligonucleotide adapters that are coupled via cleavable linkers. Incorporation of the modified nucleotide at a 3′ end of a nucleic acid permits end-adapterization via ligation of a free 5′ end of the oligonucleotide adapter to a 3′ reactive group of the modified nucleotide and cleavage at the cleavable linker to liberate a free 3′ end.