公开(公告)号：US06720547B1

公开(公告)日：2004-04-13

申请号：US09936535

申请日：2001-09-14

Applicant: Milind Rajadhyaksha ,  James M. Zavislan

Inventor： Milind Rajadhyaksha ,  James M. Zavislan

IPC: H01J4014

CPC classification number: G01N21/21 ,  G01N21/47 ,  G01N21/49 ,  G01N2021/4735 ,  G01N2021/4792 ,  G02B21/0032 ,  G02B21/0048 ,  G02B21/0068 ,  G02B21/14 ,  G02B21/33 ,  G02B21/36

Abstract: A confocal scanning microscope system (10) using cross polarization effects and an enhancement agent (acetic acid) to enhance confocal microscope reflectance images of the nuclei of BCCs (basal cell carcinomas) and SCCs (squamous call carcinomas) in the confocal reflectance images of excised tumor slices. The confocal scanning microscope system having a laser (11) for generating an illumination beam (12), a polygon mirror (18) for scanning the beam to a tissue sample (22) and for receiving a return beam from the tissue sample and detector (28) for detecting the returned beam to form an image. The system further includes a half-waveplate (13) having a rotatable stage (14) and a quarter-wave plate (21) having a rotatable stage (20) disposed in the optical path of the illumination beam and at least a linear polarizer (24) having a rotatable stage (25) disposed in the optical path of the returned beam from the tissue sample.

Abstract translation: 使用交叉极化效应的共焦扫描显微镜系统（10）和增强剂（乙酸）以增强切除的共聚焦反射图像中BCC（基底细胞癌）和SCC（鳞状细胞癌）的细胞核的共聚焦显微镜反射图像 肿瘤切片。 具有用于产生照明光束的激光器（11）的共焦扫描显微镜系统，用于将光束扫描到组织样本（22）并用于从组织样本和检测器（22）接收返回光束的多面镜（18） 28），用于检测返回的光束以形成图像。 该系统还包括具有可旋转台（14）和四分之一波片（21）的半波片（13），其具有设置在照明光束的光路中的可旋转台（20）和至少一个线性偏振器 24）具有设置在来自组织样本的返回光束的光路中的可旋转台（25）。

公开(公告)号：US06413252B1

公开(公告)日：2002-07-02

申请号：US09256145

申请日：1999-02-24

Applicant: James M. Zavislan

Inventor： James M. Zavislan

IPC: A61B1818

CPC classification number: G02B21/0056 ,  A61B18/02 ,  A61B90/20 ,  A61B2018/00041 ,  G01N1/42 ,  G02B21/0028 ,  G02B21/34

Abstract: A confocal microscope is provided including a confocal imaging system having an objective lens, and a device which can be an attachment to the confocal imaging system, or a part thereof. The device has a housing with an interior cavity and two opposite ends, where one end of the housing receives the objective lens, and at the other end of the housing which is attached to, or is a part of, the housing which forms that other end. The chamber has first and second plates presenting two opposing sides of the chamber, where the first plate lies adjacent the tissue to be treated, and the second plate is attached to the housing. The interior cavity of the housing has a window defining a first volume between the window and the objective lens, and a second volume between the window and the chamber. The first volume has an immersion liquid optically matched to the objective lens, and the second volume is approximately evacuated. The confocal imaging system, via the device, focuses light into the tissue and collects returned light from the tissue to produce images representing one or more sections of the tissue. To freeze the tissue, the chamber receives a cryogenic fluid, such as liquid nitrogen, thereby cryosurgically treating the tissue below the chamber which includes the imaged tissue. The chamber has an input port to receive the cryogenic fluid and an output port to vent the cryogenic fluid. The confocal microscope can provide images of the tissue before, during and after cryosurgical treatment.

Abstract translation: 提供一种共焦显微镜，包括具有物镜的共聚焦成像系统，以及可以是与共聚焦成像系统或其一部分的附件的装置。 该装置具有壳体，其具有内部空腔和两个相对的端部，其中壳体的一端接收物镜，并且在壳体的另一端处，该壳体附接到形成该另一个的壳体的一部分或者是壳体的一部分 结束。 该腔室具有呈现室的两个相对侧的第一和第二板，其中第一板位于待处理组织附近，并且第二板附接到壳体。 壳体的内部空腔具有限定窗口和物镜之间的第一容积的窗口，以及窗口和室之间的第二容积。 第一体积具有与物镜光学匹配的浸没液体，并且第二体积近似抽真空。 共聚焦成像系统通过该装置将光聚焦到组织中并收集来自组织的返回光以产生表示组织的一个或多个部分的图像。 为了冷冻组织，腔室接收诸如液氮的低温流体，从而对包括成像组织的腔室下方的组织进行冷冻治疗。 该室具有用于接收低温流体的输入端口和用于排出低温流体的输出端口。 共焦显微镜可以在冷冻治疗之前，期间和之后提供组织的图像。

公开(公告)号：US06411434B1

公开(公告)日：2002-06-25

申请号：US09502252

申请日：2000-02-17

Applicant: Jay M. Eastman ,  William J. Fox ,  Roger J. Greenwald ,  Kevin P. Roesser ,  James M. Zavislan

Inventor： Jay M. Eastman ,  William J. Fox ,  Roger J. Greenwald ,  Kevin P. Roesser ,  James M. Zavislan

IPC: G02B2134

CPC classification number: G02B21/368 ,  B01L3/508 ,  B01L2200/0642 ,  B01L2300/021 ,  B01L2300/043 ,  B01L2300/044 ,  B01L2300/0609 ,  B01L2300/0822 ,  B01L2300/123 ,  G01N2001/315 ,  G02B21/0012 ,  G02B21/26 ,  G02B21/33 ,  G02B21/34

Abstract: A cassette for retaining a specimen of surgically exposed tissue from a patient in an orientation that facilitates optical sectioning of the tissue by a confocal microscopic or other optical imaging microscope. The cassette includes a base member having a rigid optically transparent window upon which a tissue specimen is situated, a pliable membrane locatable over a substantial portion of the base member including the window, and an upper member, having an aperture therethrough, which can cover the base member to provide an enclosed cavity between the membrane and the window sealing the tissue specimen therein. With the tissue specimen in the enclosed cavity of the cassette, the edges of the tissue specimen may be positioned planar against the window and retained in that position by bonds formed between the membrane and window at multiple points or locations around the tissue specimen. The specimen retained in the cavity is viewable through the aperture of the upper member and imagible by a confocal microscope, or other optical imaging microscope, through the window of the base member. The base member has an injection port through which fluid may be inserted and removed from the cavity of the cassette in which the tissue specimen is retained. Such fluids can facilitate imaging of the specimen by an optical imaging microscope, or can be used to place the specimen in a preservative for archival purposes. A label with indicia identifying the tissue specimen may be applied to the cassette. The cassette may be part of a confocal imaging system to produce microscopic images of sections of the tissue specimen useful for Mohs surgery.

Abstract translation: 用于通过共聚焦显微镜或其他光学成像显微镜以方便光学切片的方向从患者保持外科手术曝光的组织的样本的盒。 盒包括具有刚性光学透明窗口的基部构件，组织样本位于该基部构件上，柔性膜可定位在包括窗口的基部构件的主要部分上方，以及具有穿过其的孔的上部构件，其可覆盖 在膜和窗户之间提供封闭的腔，密封组织样本。 利用组织样本在盒的封闭空腔中，组织样本的边缘可以相对于窗口定位成平行并且通过在组织样本周围的多个点或位置处形成在膜和窗之间的结合保持在该位置。 通过上部构件的孔可以通过共焦显微镜或其他光学成像显微镜通过基底构件的窗口观察保留在空腔中的样本。 基部构件具有注射口，流体可以通过该注入口从其中保留有组织标本的盒的腔中被插入和移除。 这种流体可以促进通过光学成像显微镜对样品进行成像，或者可以将样品放置在防腐剂中用于归档目的。 具有识别组织样本的标记的标签可以应用于盒。 盒可以是共焦成像系统的一部分，以产生用于莫氏手术的组织标本的切片的显微镜图像。

公开(公告)号：US5653706A

公开(公告)日：1997-08-05

申请号：US395223

申请日：1995-02-27

Applicant: James M. Zavislan ,  Jay M. Eastman

Inventor： James M. Zavislan ,  Jay M. Eastman

IPC: A61B17/00 ,  A61B18/20 ,  A61B19/00 ,  A61N5/06 ,  A61B17/36

CPC classification number: A61B18/203 ,  A61B18/20 ,  A61B2017/00756 ,  A61B2018/00452 ,  A61B2018/00476 ,  A61B2018/00904 ,  A61B2018/2095 ,  A61B2090/373

Abstract: A hand held microsurgical instrument for applying laser energy to selected locations (sites) in an area under the skin (or other exposed translucent tissue) to provide localized photothermolysis of underlying tissue at these sites, is described. The laser energy is focused into a spot within the tissue. This spot is of sufficiently small size so that the energy density is sufficient to provide surgical or treatment effects within the tissue without damaging the surface tissue. In dermatology, for example, the technique can be used to destroy endothelial cells in blood vessels which are desired to be removed, such spider veins (nevi) in the skin, hair follicles to prevent hair growth therefrom, or other microsurgical procedures. The area is visualized while the laser beam is steered, using a deflection system, in X and Y coordinates. A telecentric optical system, in which a mirror of the deflection system is located, directs the laser light essentially perpendicular to the area to be treated as the beam is scanned over the area. The optical system also focuses illumination light reflected from the area to a sensor matrix of a CCD video camera. The reflected illumination light is imaged essentially parallel to the optical axis in the object space thereby providing a precise, high resolution image corresponding to the area. The laser beam may be tracked as it is deflected over the area to the selected locations by visualization thereof on a display or monitor associated with the video camera. The locations are then apparent to the treating physician who can then effect an increase of the beam power or turn the beam on so as to treat the tissue in the selected locations.

Abstract translation: 描述了一种用于将激光能量施加到皮肤下面的区域（或其他暴露的半透明组织）中的选定位置（位置）以在这些位置处提供局部光热解下面的组织的手持显微外科器械。 激光能量集中在组织内的一个点上。 该斑点具有足够小的尺寸，使得能量密度足以在组织内提供手术或治疗效果而不损伤表面组织。 例如，在皮肤病学中，该技术可用于破坏需要去除的血管中的内皮细胞，皮肤中的这种蜘蛛静脉（痣），毛囊以防止毛发生长或其它显微外科手术。 在X和Y坐标下，使用偏转系统对激光束进行转向时可视化该区域。 偏转系统的反射镜位于其中的远心光学系统在该区域上扫描光束时将激光基本上垂直于待处理的区域引导。 光学系统还将从该区域反射的照明光聚焦到CCD摄像机的传感器矩阵。 反射的照明光基本上平行于对象空间中的光轴成像，从而提供对应于该区域的精确的高分辨率图像。 激光束可以被跟踪，因为它通过在与摄像机相关联的显示器或监视器上的可视化而在区域上偏转到所选择的位置。 然后，这些位置对于治疗医师是显而易见的，其然后可以实现光束功率的增加或者使光束转动，以便在所选择的位置处理组织。

公开(公告)号：US5632741A

公开(公告)日：1997-05-27

申请号：US376205

申请日：1995-01-20

Applicant: James M. Zavislan ,  Steven H. Tomson, deceased

Inventor： James M. Zavislan ,  Steven H. Tomson, deceased

IPC: A61B18/20 ,  A61N5/06 ,  A61B17/00

CPC classification number: A61B18/203 ,  A61B2018/00452 ,  A61B2018/00476

Abstract: In order to permanently remove hair growing in subcutaneous (below the skin's surface) tissue and to do so permanently, the hair and its root structure is epilated (vaporized) in an ablation cavity of essentially the same size and shape as the volume occupied by the root structure which includes follicle, the follicle bulb and the dermal papilla. Optical energy is used which is of a wavelength and fluence sufficient to cause epilation in the cavity without relying upon selective photothermolysis thereby limiting damage to tissue outside of the hair root structure. To find and restrict the optical energy to the epilation cavity, an optical system, which may be constituted of a single lens, having a numerical aperture of F/5-5.8 is used. The beam is generated by a laser which may be operated in a pulse mode and contained in a hand piece having the optical system at the end thereof. In order to facilitate epilation, a medium such as a cold compress or a freezing agent, may be applied before application of the laser energy in order to cause the root structure and the hair to move to a location generally perpendicular to the surface of the skin. During application of the laser energy, the root structure and the hair, if present, is progressively ablated and vaporized until the bottom of the hair bulb is reached at which time the beam is turned off. Because of the shaping effect of the optical system, the beam is diffused so as to have insufficient fluence to damage the tissue below the bottom of the hair follicle bulb.

Abstract translation: 为了永久地去除在皮下（皮肤表面以下）组织生长的毛发并永久地进行这种处理，毛发及其根部结构在消融腔中被脱毛（蒸发），其消融腔的尺寸和形状基本上与由 根结构包括卵泡，卵泡和真皮乳头。 使用光能，其具有足以在腔中引起脱毛的波长和注量，而不依赖于选择性光热解，从而限制对发根结构外的组织的损伤。 为了找到并限制到脱毛腔的光能，可以使用具有数值孔径为F / 5-5.8的单个透镜构成的光学系统。 光束由激光器产生，该激光器可以以脉冲模式操作并且包含在其末端具有光学系统的手持部件中。 为了促进脱毛，可以在施加激光能量之前施加诸如冷敷或冷冻剂的介质，以便使根部结构和毛发移动到大致垂直于皮肤表面的位置 。 在施加激光能量的过程中，根部结构和毛发（如果存在）被逐渐消融并蒸发，直到达到头发的底部，此时光束被关闭。 由于光学系统的成形效果，光束被扩散以具有不足的注量以损坏毛囊的底部以下的组织。

公开(公告)号：US5537617A

公开(公告)日：1996-07-16

申请号：US279455

申请日：1994-07-22

Applicant: James M. Zavislan ,  Bulent N. Kurdi

Inventor： James M. Zavislan ,  Bulent N. Kurdi

IPC: G11B7/12 ,  G11B7/14 ,  G02B6/34

CPC classification number: G11B7/14 ,  G11B7/124

Abstract: A multichannel optical head and data storage system employs an integrated-optical read channel. The read channel may be fabricated on a planar waveguide structure that separates the data signals of the multiple lasers. The read channel includes focus error sensors and track error sensors. The optical head may be used for amplitude recording systems such as phase-change erasable and write-once media, ablation media, dye-polymer media, and OD-ROM systems.

Abstract translation: 多通道光头和数据存储系统采用集成光读取通道。 读通道可以在分离多个激光器的数据信号的平面波导结构上制造。 读通道包括聚焦误差传感器和轨道误差传感器。 光头可用于振幅记录系统，例如相变可擦除和一次写入介质，消融介质，染料聚合物介质和OD-ROM系统。

公开(公告)号：US5416757A

公开(公告)日：1995-05-16

申请号：US245881

申请日：1994-05-18

Applicant: Frank Luecke ,  James M. Zavislan

Inventor： Frank Luecke ,  James M. Zavislan

IPC: G11B7/085 ,  G11B7/00 ,  G11B7/135 ,  G11B7/20

CPC classification number: G11B7/13925 ,  G11B7/1369 ,  G11B2007/0006 ,  G11B7/0948

Abstract: The objective lens of an optical disk drive has a suitable positioning and numerical aperture so that the light beam will be focused into a spot on the optical media of a first optical disk having a cover plate with a first thickness. When a second optical disk is utilized having a cover plate with a second thickness different from the first thickness, a liquid crystal compensator lens is actuated in the optical path between the light source and the objective lens for focusing the light beam into a spot on the optical media of the second optical disk. A correction signal may be applied to the liquid crystal in the compensator lens for altering a refractive index thereof. This substantially eliminates a wavefront aberration in the reflected light beam which would otherwise occur if the beam were focused onto the optical media of the second optical disk solely by use of the objective lens. The lower alignment film of the liquid crystal compensator lens has a cross-section which is approximately sinusoidal in order to complement three waves of aberration (measured from peak-to-valley) in the reflected wavefront.

Abstract translation: 光盘驱动器的物镜具有适当的定位和数值孔径，使得光束将被聚焦到具有第一厚度的盖板的第一光盘的光学介质上的光点中。 当使用具有不同于第一厚度的第二厚度的盖板的第二光盘时，在光源和物镜之间的光路中致动液晶补偿透镜，以将光束聚焦到 第二光盘的光学介质。 校正信号可以应用于补偿透镜中的液晶以改变其折射率。 这基本上消除了仅仅通过使用物镜将光束聚焦到第二光盘的光学介质上否则会发生反射光束的波前像差。 液晶补偿透镜的下取向膜具有近似正弦的横截面，以便补偿反射波前的三波像差（从峰到谷测量）。

公开(公告)号：US5247167A

公开(公告)日：1993-09-21

申请号：US927888

申请日：1992-08-06

Applicant: Richard A. Bargerhuff ,  LeRoy D. Dickson ,  John Groot ,  Melbourne E. Rabedeau ,  James M. Zavislan

Inventor： Richard A. Bargerhuff ,  LeRoy D. Dickson ,  John Groot ,  Melbourne E. Rabedeau ,  James M. Zavislan

IPC: G11B7/12 ,  G11B7/125 ,  G11B11/105 ,  H01S3/00 ,  H01S5/00

CPC classification number: G11B7/127 ,  G11B11/10532 ,  G11B11/10595 ,  G11B7/123 ,  G11B7/126 ,  H01S3/005 ,  H01S5/0014 ,  H01S5/005

Abstract: A multiple beam optical system includes a housing which contains a pair of laser diodes and a pair of optical detectors. The lasers produce overlapping beams of light. The housing includes a beam directing and reflecting member which selectively focuses light from one of the lasers to a corresponding optical detector located in the housing and selectively focuses light from the other laser to its corresponding optical detector located within the housing. The individual power of each of the overlapping laser beams is thereby monitored by the separate detectors.

Abstract translation: 多光束光学系统包括包含一对激光二极管和一对光学检测器的壳体。 激光产生重叠的光束。 该外壳包括一个光束引导和反射构件，该光束引导和反射构件选择性地将来自激光器之一的光聚焦到位于壳体中的对应的光学检测器，并且将来自另一激光器的光选择性地聚焦到位于壳体内的相应的光学检测器。 因此，通过单独的检测器来监视每个重叠的激光束的各自的功率。

公开(公告)号：US20130182318A1

公开(公告)日：2013-07-18

申请号：US13385980

申请日：2012-03-19

Applicant: Jay M. Eastman ,  William J. Fox ,  Roger J. Greenwald ,  Kevin P. Roesser ,  James M. Zavislan

Inventor： Jay M. Eastman ,  William J. Fox ,  Roger J. Greenwald ,  Kevin P. Roesser ,  James M. Zavislan

IPC: G02B21/34

CPC classification number: G02B21/368 ,  B01L3/508 ,  B01L2200/0642 ,  B01L2300/021 ,  B01L2300/043 ,  B01L2300/044 ,  B01L2300/0609 ,  B01L2300/0822 ,  B01L2300/123 ,  G01N2001/315 ,  G02B21/0012 ,  G02B21/26 ,  G02B21/33 ,  G02B21/34

Abstract: A cassette for retaining a specimen of surgically exposed tissue from a patient in an orientation that facilitates optical sectioning of the tissue by a confocal microscopic or other optical imaging microscope. The cassette includes a base member having a rigid optically transparent window upon which a tissue specimen is situated, a pliable membrane locatable over a substantial portion of the base member including the window, and an upper member, having an aperture therethrough, which can cover the base member to provide an enclosed cavity between the membrane and the window sealing the tissue specimen therein. The edges of the tissue specimen may be positioned planar against the window and retained in that position by bonds formed between the membrane and window at multiple points or locations around the tissue specimen. The specimen retained in the cavity is imagible a microscope through the window of the base member.

Abstract translation: 用于通过共聚焦显微镜或其他光学成像显微镜以方便光学切片的方向从患者保持外科手术曝光的组织的样本的盒。 盒包括具有刚性光学透明窗口的基部构件，组织样本位于该基部构件上，柔性膜可定位在包括窗口的基部构件的主要部分上方，以及具有穿过其的孔的上部构件，其可覆盖 在膜和窗户之间提供封闭的腔，密封组织样本。 组织样本的边缘可以相对于窗户定位，并且通过在组织样本周围的多个点或位置处形成在膜和窗之间的结合保持在该位置。 保留在腔中的样品通过基底构件的窗口成像显微镜。

公开(公告)号：US08121670B2

公开(公告)日：2012-02-21

申请号：US11081181

申请日：2005-03-16

Applicant: James M. Zavislan

Inventor： James M. Zavislan

IPC: A61B6/00

CPC classification number: A61B5/6842 ,  A61B5/0068 ,  A61B5/061 ,  A61B5/441 ,  A61B5/444 ,  A61B5/445 ,  A61B5/6834 ,  A61B5/6835 ,  A61B5/6843 ,  A61B5/704

Abstract: An improved system for confocal imaging within dermal tissue of a patient is provided which minimizes instability in confocal images by reducing the relative motion of the tissue with respect to the confocal imaging optics of the system. The system includes a mechanism for maintaining an area of skin tissue under stress by application of force at the edges of the area, and an imaging head coupled to this mechanism for imaging the stressed skin. The mechanism includes a mechanical structure, such as a platen, brace, or attachment, which both supports the imaging head of the system and applies stress to a limited surface area of the tissue to minimize skin motion during confocal imaging.

Abstract translation: 提供了一种用于患者真皮组织内的共焦成像的改进系统，其通过减少组织相对于系统的共聚焦成像光学器件的相对运动而使共焦图像中的不稳定性最小化。 该系统包括用于通过在该区域的边缘处施加力来保持应力下的皮肤组织的面积的机构，以及耦合到该机构的用于对应力皮肤成像的成像头。 该机构包括机械结构，例如压板，支架或附件，其均支撑系统的成像头部，并将应力施加到组织的有限表面区域，以使共聚焦成像期间的皮肤运动最小化。